Details of the Target

General Information of Target

Target ID LDTP04033
Target Name Translocon-associated protein subunit alpha (SSR1)
Gene Name SSR1
Gene ID 6745
Synonyms
TRAPA; Translocon-associated protein subunit alpha; TRAP-alpha; Signal sequence receptor subunit alpha; SSR-alpha
3D Structure
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2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MRLLPRLLLLLLLVFPATVLFRGGPRGLLAVAQDLTEDEETVEDSIIEDEDDEAEVEEDE
PTDLVEDKEEEDVSGEPEASPSADTTILFVKGEDFPANNIVKFLVGFTNKGTEDFIVESL
DASFRYPQDYQFYIQNFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDL
NGNVFQDAVFNQTVTVIEREDGLDGETIFMYMFLAGLGLLVIVGLHQLLESRKRKRPIQK
VEMGTSSQNDVDMSWIPQETLNQINKASPRRLPRKRAQKRSVGSDE
Target Bioclass
Transporter and channel
Family
TRAP-alpha family
Subcellular location
Endoplasmic reticulum membrane
Function
TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.
Uniprot ID
P43307
Ensemble ID
ENST00000244763.9
HGNC ID
HGNC:11323

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO678 SNV: p.F15S .
FUOV1 SNV: p.Q239Ter .
KMCH1 SNV: p.V282L .
YSCCC SNV: p.R2T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		9.80  LDD0318  [1]
STPyne
 Probe Info  		K235(8.28)  LDD0277  [2]
HPAP
 Probe Info  		4.42  LDD0062  [3]
m-APA
 Probe Info  		N.A.  LDD2233  [4]
1c-yne
 Probe Info  		K266(0.00); K102(0.00)  LDD0228  [5]
PAL-AfBPP Probe
Click To Hide/Show 187 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C001
 Probe Info  		6.41  LDD1711  [6]
C002
 Probe Info  		6.45  LDD1712  [6]
C004
 Probe Info  		15.24  LDD1714  [6]
C007
 Probe Info  		9.25  LDD1716  [6]
C008
 Probe Info  		7.11  LDD1717  [6]
C010
 Probe Info  		8.57  LDD1719  [6]
C011
 Probe Info  		6.36  LDD1720  [6]
C017
 Probe Info  		18.25  LDD1725  [6]
C022
 Probe Info  		99.73  LDD1728  [6]
C026
 Probe Info  		22.94  LDD1732  [6]
C027
 Probe Info  		5.62  LDD1733  [6]
C039
 Probe Info  		12.04  LDD1739  [6]
C040
 Probe Info  		99.73  LDD1740  [6]
C041
 Probe Info  		26.54  LDD1741  [6]
C049
 Probe Info  		11.88  LDD1747  [6]
C051
 Probe Info  		6.15  LDD1749  [6]
C052
 Probe Info  		5.17  LDD1750  [6]
C053
 Probe Info  		12.55  LDD1751  [6]
C055
 Probe Info  		46.53  LDD1752  [6]
C056
 Probe Info  		99.73  LDD1753  [6]
C060
 Probe Info  		6.59  LDD1757  [6]
C063
 Probe Info  		41.64  LDD1760  [6]
C064
 Probe Info  		9.13  LDD1761  [6]
C067
 Probe Info  		6.59  LDD1763  [6]
C070
 Probe Info  		27.28  LDD1766  [6]
C072
 Probe Info  		16.22  LDD1768  [6]
C082
 Probe Info  		16.22  LDD1774  [6]
C085
 Probe Info  		6.82  LDD1777  [6]
C087
 Probe Info  		15.35  LDD1779  [6]
C091
 Probe Info  		82.14  LDD1782  [6]
C092
 Probe Info  		99.73  LDD1783  [6]
C094
 Probe Info  		99.73  LDD1785  [6]
C095
 Probe Info  		18.51  LDD1786  [6]
C100
 Probe Info  		19.84  LDD1789  [6]
C106
 Probe Info  		99.73  LDD1793  [6]
C107
 Probe Info  		16.00  LDD1794  [6]
C108
 Probe Info  		29.86  LDD1795  [6]
C112
 Probe Info  		99.73  LDD1799  [6]
C129
 Probe Info  		8.51  LDD1811  [6]
C130
 Probe Info  		17.39  LDD1812  [6]
C131
 Probe Info  		6.77  LDD1813  [6]
C134
 Probe Info  		99.73  LDD1816  [6]
C135
 Probe Info  		29.86  LDD1817  [6]
C139
 Probe Info  		15.14  LDD1821  [6]
C140
 Probe Info  		9.71  LDD1822  [6]
C141
 Probe Info  		29.86  LDD1823  [6]
C143
 Probe Info  		66.72  LDD1825  [6]
C147
 Probe Info  		8.17  LDD1829  [6]
C153
 Probe Info  		59.30  LDD1834  [6]
C158
 Probe Info  		14.72  LDD1838  [6]
C159
 Probe Info  		34.30  LDD1839  [6]
C160
 Probe Info  		26.35  LDD1840  [6]
C161
 Probe Info  		51.98  LDD1841  [6]
C163
 Probe Info  		15.03  LDD1843  [6]
C165
 Probe Info  		21.56  LDD1845  [6]
C166
 Probe Info  		12.13  LDD1846  [6]
C169
 Probe Info  		99.73  LDD1849  [6]
C170
 Probe Info  		10.63  LDD1850  [6]
C172
 Probe Info  		5.94  LDD1852  [6]
C173
 Probe Info  		8.57  LDD1853  [6]
C174
 Probe Info  		6.87  LDD1854  [6]
C177
 Probe Info  		10.20  LDD1856  [6]
C178
 Probe Info  		56.10  LDD1857  [6]
C183
 Probe Info  		6.63  LDD1861  [6]
C186
 Probe Info  		21.26  LDD1864  [6]
C187
 Probe Info  		56.49  LDD1865  [6]
C191
 Probe Info  		21.71  LDD1868  [6]
C193
 Probe Info  		8.88  LDD1869  [6]
C194
 Probe Info  		25.11  LDD1870  [6]
C196
 Probe Info  		35.02  LDD1872  [6]
C197
 Probe Info  		5.31  LDD1873  [6]
C198
 Probe Info  		27.86  LDD1874  [6]
C201
 Probe Info  		99.73  LDD1877  [6]
C204
 Probe Info  		6.23  LDD1879  [6]
C206
 Probe Info  		88.03  LDD1881  [6]
C207
 Probe Info  		33.36  LDD1882  [6]
C210
 Probe Info  		99.73  LDD1884  [6]
C211
 Probe Info  		9.58  LDD1885  [6]
C213
 Probe Info  		33.36  LDD1887  [6]
C214
 Probe Info  		6.45  LDD1888  [6]
C218
 Probe Info  		49.87  LDD1892  [6]
C219
 Probe Info  		9.38  LDD1893  [6]
C220
 Probe Info  		41.36  LDD1894  [6]
C225
 Probe Info  		15.67  LDD1898  [6]
C226
 Probe Info  		13.64  LDD1899  [6]
C228
 Probe Info  		99.73  LDD1901  [6]
C229
 Probe Info  		17.15  LDD1902  [6]
C231
 Probe Info  		99.73  LDD1904  [6]
C232
 Probe Info  		99.73  LDD1905  [6]
C233
 Probe Info  		25.63  LDD1906  [6]
C234
 Probe Info  		20.82  LDD1907  [6]
C235
 Probe Info  		99.73  LDD1908  [6]
C238
 Probe Info  		54.57  LDD1911  [6]
C240
 Probe Info  		18.51  LDD1913  [6]
C243
 Probe Info  		53.82  LDD1916  [6]
C244
 Probe Info  		58.08  LDD1917  [6]
C245
 Probe Info  		11.00  LDD1918  [6]
C246
 Probe Info  		88.65  LDD1919  [6]
C249
 Probe Info  		36.25  LDD1922  [6]
C251
 Probe Info  		30.48  LDD1924  [6]
C252
 Probe Info  		32.90  LDD1925  [6]
C258
 Probe Info  		8.94  LDD1931  [6]
C260
 Probe Info  		4.92  LDD1932  [6]
C264
 Probe Info  		82.71  LDD1935  [6]
C265
 Probe Info  		52.71  LDD1936  [6]
C266
 Probe Info  		9.00  LDD1937  [6]
C269
 Probe Info  		8.57  LDD1939  [6]
C270
 Probe Info  		17.75  LDD1940  [6]
C271
 Probe Info  		7.36  LDD1941  [6]
C273
 Probe Info  		11.08  LDD1943  [6]
C277
 Probe Info  		24.42  LDD1947  [6]
C278
 Probe Info  		99.73  LDD1948  [6]
C280
 Probe Info  		10.63  LDD1950  [6]
C282
 Probe Info  		42.22  LDD1952  [6]
C284
 Probe Info  		44.32  LDD1954  [6]
C285
 Probe Info  		99.73  LDD1955  [6]
C286
 Probe Info  		5.90  LDD1956  [6]
C287
 Probe Info  		41.64  LDD1957  [6]
C288
 Probe Info  		22.32  LDD1958  [6]
C289
 Probe Info  		99.73  LDD1959  [6]
C290
 Probe Info  		8.11  LDD1960  [6]
C292
 Probe Info  		5.24  LDD1962  [6]
C293
 Probe Info  		99.73  LDD1963  [6]
C296
 Probe Info  		52.71  LDD1966  [6]
C299
 Probe Info  		29.65  LDD1968  [6]
C301
 Probe Info  		5.10  LDD1970  [6]
C304
 Probe Info  		5.94  LDD1973  [6]
C305
 Probe Info  		30.48  LDD1974  [6]
C307
 Probe Info  		7.06  LDD1975  [6]
C310
 Probe Info  		28.64  LDD1977  [6]
C313
 Probe Info  		26.54  LDD1980  [6]
C314
 Probe Info  		40.50  LDD1981  [6]
C317
 Probe Info  		10.41  LDD1983  [6]
C322
 Probe Info  		14.62  LDD1988  [6]
C326
 Probe Info  		24.08  LDD1990  [6]
C338
 Probe Info  		24.42  LDD2001  [6]
C339
 Probe Info  		21.71  LDD2002  [6]
C343
 Probe Info  		49.87  LDD2005  [6]
C346
 Probe Info  		11.16  LDD2007  [6]
C347
 Probe Info  		5.28  LDD2008  [6]
C348
 Probe Info  		47.84  LDD2009  [6]
C349
 Probe Info  		99.73  LDD2010  [6]
C350
 Probe Info  		99.73  LDD2011  [6]
C353
 Probe Info  		12.13  LDD2014  [6]
C354
 Probe Info  		15.67  LDD2015  [6]
C355
 Probe Info  		87.43  LDD2016  [6]
C356
 Probe Info  		25.63  LDD2017  [6]
C357
 Probe Info  		12.30  LDD2018  [6]
C361
 Probe Info  		20.11  LDD2022  [6]
C362
 Probe Info  		99.73  LDD2023  [6]
C363
 Probe Info  		33.13  LDD2024  [6]
C364
 Probe Info  		53.08  LDD2025  [6]
C366
 Probe Info  		18.13  LDD2027  [6]
C367
 Probe Info  		10.06  LDD2028  [6]
C373
 Probe Info  		21.41  LDD2033  [6]
C376
 Probe Info  		21.11  LDD2036  [6]
C378
 Probe Info  		12.55  LDD2037  [6]
C380
 Probe Info  		7.52  LDD2039  [6]
C382
 Probe Info  		37.27  LDD2041  [6]
C383
 Probe Info  		34.06  LDD2042  [6]
C386
 Probe Info  		14.62  LDD2045  [6]
C388
 Probe Info  		99.73  LDD2047  [6]
C390
 Probe Info  		99.73  LDD2049  [6]
C391
 Probe Info  		54.19  LDD2050  [6]
C399
 Probe Info  		18.77  LDD2058  [6]
C403
 Probe Info  		54.57  LDD2061  [6]
C407
 Probe Info  		44.32  LDD2064  [6]
C409
 Probe Info  		5.28  LDD2066  [6]
C413
 Probe Info  		29.65  LDD2069  [6]
C419
 Probe Info  		22.78  LDD2074  [6]
C420
 Probe Info  		22.78  LDD2075  [6]
C424
 Probe Info  		20.82  LDD2079  [6]
C426
 Probe Info  		46.53  LDD2081  [6]
C429
 Probe Info  		32.90  LDD2084  [6]
C431
 Probe Info  		73.52  LDD2086  [6]
FFF probe11
 Probe Info  		20.00  LDD0471  [7]
FFF probe13
 Probe Info  		20.00  LDD0475  [7]
FFF probe14
 Probe Info  		20.00  LDD0477  [7]
FFF probe2
 Probe Info  		20.00  LDD0463  [7]
FFF probe3
 Probe Info  		20.00  LDD0464  [7]
FFF probe4
 Probe Info  		20.00  LDD0466  [7]
JN0003
 Probe Info  		20.00  LDD0469  [7]
STS-1
 Probe Info  		N.A.  LDD0136  [8]
STS-2
 Probe Info  		N.A.  LDD0138  [8]
VE-P
 Probe Info  		N.A.  LDD0396  [9]
Alk-rapa
 Probe Info  		6.03  LDD0213  [10]
OEA-DA
 Probe Info  		20.00  LDD0046  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0014  Panhematin HEK-293T 4.42  LDD0062  [3]
 LDCM0090  Rapamycin JHH-7 6.03  LDD0213  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Calnexin (CANX) Calreticulin family P27824
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-1-antitrypsin (SERPINA1) Serpin family P01009

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
7 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
8 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
9 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
10 Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth. Cell Chem Biol. 2022 Mar 17;29(3):373-385.e6. doi: 10.1016/j.chembiol.2021.10.006. Epub 2021 Oct 26.
11 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570