Details of the Probe

General Information of Probe

Probe ID
LDPC0548
Probe Name
C322
Synonyms
Z4286591579
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 403.9
Lipid-water partition coefficient (xlogp) 2
Rotatable Bond Count (rotbonds) 8
Hydrogen Bond Donor Count (hbonddonor) 2
Hydrogen Bond Acceptor Count (hbondacc) 5
Chemical Identifiers
Formula
C19H22ClN5O3
IUPAC Name
3-N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]-1-N-(5-chloro-2-methoxyphenyl)azetidine-1,3-dicarboxamide
Canonical SMILES
COC1=C(C=C(C=C1)Cl)NC(=O)N2CC(C2)C(=O)NCCC3(N=N3)CCC#C
InChI
InChI=1S/C19H22ClN5O3/c1-3-4-7-19(23-24-19)8-9-21-17(26)13-11-25(12-13)18(27)22-15-10-14(20)5-6-16(15)28-2/h1,5-6,10,13H,4,7-9,11-12H2,2H3,(H,21,26)(H,22,27)
InChIKey
LBQSMDYDTSDDHB-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

78 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP) LDTP06325 9.13  LDD1988 
7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) LDTP10032 6.15  LDD1988 
Acetyl-CoA acetyltransferase, cytosolic (ACAT2) LDTP11321 6.87  LDD1988 
ADP-dependent glucokinase (ADPGK) LDTP11127 16.34  LDD1988 
Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) LDTP04437 5.74  LDD1988 
Aspartyl/asparaginyl beta-hydroxylase (ASPH) LDTP05652 5.70  LDD1988 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 10.41  LDD1988 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 7.73  LDD1988 
Ceramide synthase 2 (CERS2) LDTP10329 7.26  LDD1988 
Choline kinase alpha (CHKA) LDTP03787 9.65  LDD1988 
Cytochrome b-c1 complex subunit 8 (UQCRQ) LDTP00486 10.41  LDD1988 
Cytochrome b-c1 complex subunit 9 (UQCR10) LDTP13189 10.78  LDD1988 
Cytochrome b5 type B (CYB5B) LDTP00697 22.32  LDD1988 
Cytochrome P450 20A1 (CYP20A1) LDTP17410 10.41  LDD1988 
dCTP pyrophosphatase 1 (DCTPP1) LDTP12013 6.92  LDD1988 
Delta(14)-sterol reductase LBR (LBR) LDTP06200 8.88  LDD1988 
Delta(24)-sterol reductase (DHCR24) LDTP06375 7.67  LDD1988 
DNA replication licensing factor MCM3 (MCM3) LDTP03316 5.74  LDD1988 
E3 ubiquitin-protein ligase TRIM21 (TRIM21) LDTP03001 6.28  LDD1988 
Endoplasmic reticulum aminopeptidase 1 (ERAP1) LDTP12868 7.46  LDD1988 
Endoplasmic reticulum metallopeptidase 1 (ERMP1) LDTP08017 7.21  LDD1988 
Endoplasmic reticulum transmembrane helix translocase (ATP13A1) LDTP12279 14.62  LDD1988 
Endothelin-converting enzyme 1 (ECE1) LDTP04008 7.31  LDD1988 
Enoyl-CoA hydratase, mitochondrial (ECHS1) LDTP03518 5.86  LDD1988 
Epimerase family protein SDR39U1 (SDR39U1) LDTP16070 6.50  LDD1988 
Epoxide hydrolase 1 (EPHX1) LDTP02175 10.41  LDD1988 
ERO1-like protein alpha (ERO1A) LDTP10386 5.90  LDD1988 
Glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2) LDTP04032 8.57  LDD1988 
Glyoxalase domain-containing protein 4 (GLOD4) LDTP16040 21.26  LDD1988 
Heme oxygenase 2 (HMOX2) LDTP03540 11.71  LDD1988 
Holocytochrome c-type synthase (HCCS) LDTP04581 6.77  LDD1988 
Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) LDTP06787 8.00  LDD1988 
Lanosterol 14-alpha demethylase (CYP51A1) LDTP06648 7.01  LDD1988 
Lathosterol oxidase (SC5D) LDTP01329 6.32  LDD1988 
Lipid droplet-associated hydrolase (LDAH) LDTP16007 11.63  LDD1988 
Lysophosphatidic acid phosphatase type 6 (ACP6) LDTP12363 7.01  LDD1988 
Methylsterol monooxygenase 1 (MSMO1) LDTP06477 5.78  LDD1988 
Minor histocompatibility antigen H13 (HM13) LDTP09396 7.73  LDD1988 
Multidrug resistance-associated protein 1 (ABCC1) LDTP03671 6.02  LDD1988 
N-alpha-acetyltransferase 40 (NAA40) LDTP08265 7.62  LDD1988 
NAD(P) transhydrogenase, mitochondrial (NNT) LDTP05862 7.94  LDD1988 
NAD-dependent protein deacetylase sirtuin-2 (SIRT2) LDTP08598 5.90  LDD1988 
NADH dehydrogenase 1 alpha subcomplex subunit 11 (NDUFA11) LDTP08385 8.22  LDD1988 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 13.27  LDD1988 
NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9) LDTP14276 6.59  LDD1988 
Nicotinamide phosphoribosyltransferase (NAMPT) LDTP04051 10.78  LDD1988 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 6.73  LDD1988 
Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) LDTP12628 14.72  LDD1988 
Palmitoyltransferase ZDHHC13 (ZDHHC13) LDTP08455 7.57  LDD1988 
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) LDTP14218 27.86  LDD1988 
Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) LDTP06307 5.78  LDD1988 
Peroxisomal acyl-coenzyme A oxidase 3 (ACOX3) LDTP00589 7.26  LDD1988 
Peroxisomal trans-2-enoyl-CoA reductase (PECR) LDTP11437 6.32  LDD1988 
Phenylalanine--tRNA ligase beta subunit (FARSB) LDTP12586 6.28  LDD1988 
Phosphatidylserine decarboxylase proenzyme, mitochondrial (PISD) LDTP13220 5.70  LDD1988 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 16.11  LDD1988 
Presequence protease, mitochondrial (PITRM1) LDTP07003 5.90  LDD1988 
Prolyl 3-hydroxylase 1 (P3H1) LDTP06750 5.78  LDD1988 
Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) LDTP02679 5.98  LDD1988 
Proteasome subunit alpha type-2 (PSMA2) LDTP03331 8.57  LDD1988 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 9.19  LDD1988 
Proteasome subunit beta type-4 (PSMB4) LDTP03417 6.36  LDD1988 
Protein farnesyltransferase subunit beta (FNTB) LDTP04247 22.63  LDD1988 
Protein O-GlcNAcase (OGA) LDTP00988 6.54  LDD1988 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 7.57  LDD1988 
Puromycin-sensitive aminopeptidase (NPEPPS) LDTP04712 15.56  LDD1988 
Reticulon-4-interacting protein 1, mitochondrial (RTN4IP1) LDTP09666 6.77  LDD1988 
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) LDTP00907 20.82  LDD1988 
RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2) LDTP05588 5.66  LDD1988 
Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) LDTP15484 9.13  LDD1988 
Serum paraoxonase/arylesterase 2 (PON2) LDTP06333 6.28  LDD1988 
Signal peptidase complex catalytic subunit SEC11A (SEC11A) LDTP05066 24.08  LDD1988 
Signal peptide peptidase-like 2A (SPPL2A) LDTP09395 6.28  LDD1988 
Squalene synthase (FDFT1) LDTP03842 12.30  LDD1988 
Thioredoxin-related transmembrane protein 1 (TMX1) LDTP11874 6.06  LDD1988 
V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) LDTP14030 6.15  LDD1988 
Very-long-chain 3-oxoacyl-CoA reductase (HSD17B12) LDTP06901 7.89  LDD1988 
Xaa-Pro aminopeptidase 1 (XPNPEP1) LDTP12444 8.63  LDD1988 
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⏷ Show the Full List of 78 Enzyme
63 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1) LDTP01601 17.75  LDD1988 
Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) LDTP09328 6.32  LDD1988 
Alpha-actinin-2 (ACTN2) LDTP03768 6.54  LDD1988 
ATP synthase subunit a (MT-ATP6) LDTP01804 57.28  LDD1988 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 6.92  LDD1988 
BOS complex subunit NCLN (NCLN) LDTP10036 12.30  LDD1988 
BRI3-binding protein (BRI3BP) LDTP15648 8.34  LDD1988 
Cysteine protease ATG4B (ATG4B) LDTP14068 6.68  LDD1988 
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1) LDTP02645 6.68  LDD1988 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 16.22  LDD1988 
Derlin-1 (DERL1) LDTP11245 11.24  LDD1988 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 23.59  LDD1988 
ER membrane protein complex subunit 5 (MMGT1) LDTP08878 10.06  LDD1988 
Exocyst complex component 2 (EXOC2) LDTP10513 7.89  LDD1988 
Exportin-4 (XPO4) LDTP11607 5.82  LDD1988 
Intraflagellar transport protein 56 (IFT56) LDTP00010 5.82  LDD1988 
Lipid scramblase CLPTM1L (CLPTM1L) LDTP10499 6.11  LDD1988 
Lysosome membrane protein 2 (SCARB2) LDTP06034 7.46  LDD1988 
Membrane-associated progesterone receptor component 1 (PGRMC1) LDTP00235 8.17  LDD1988 
Membrane-associated progesterone receptor component 2 (PGRMC2) LDTP00562 14.93  LDD1988 
Metaxin-1 (MTX1) LDTP05900 18.90  LDD1988 
Metaxin-2 (MTX2) LDTP01217 8.06  LDD1988 
Methylosome subunit pICln (CLNS1A) LDTP04597 7.94  LDD1988 
Mitochondrial 2-oxodicarboxylate carrier (SLC25A21) LDTP11095 16.00  LDD1988 
Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) LDTP00865 5.74  LDD1988 
Mitochondrial dicarboxylate carrier (SLC25A10) LDTP13174 8.69  LDD1988 
Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) LDTP10913 45.57  LDD1988 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 60.55  LDD1988 
Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) LDTP14099 14.52  LDD1988 
Mitochondrial inner membrane protein OXA1L (OXA1L) LDTP06308 12.04  LDD1988 
Mitochondrial ornithine transporter 1 (SLC25A15) LDTP14200 5.62  LDD1988 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 18.38  LDD1988 
Mpv17-like protein 2 (MPV17L2) LDTP14952 9.58  LDD1988 
Nucleoporin NDC1 (NDC1) LDTP11213 6.59  LDD1988 
Nucleoporin Nup37 (NUP37) LDTP09203 6.28  LDD1988 
PAT complex subunit CCDC47 (CCDC47) LDTP10059 6.15  LDD1988 
Protein GPR107 (GPR107) LDTP07236 11.00  LDD1988 
Proton-coupled zinc antiporter SLC30A5 (SLC30A5) LDTP09294 6.02  LDD1988 
Putative lipid scramblase CLPTM1 (CLPTM1) LDTP01745 7.36  LDD1988 
Reduced folate transporter (SLC19A1) LDTP03952 5.94  LDD1988 
Reticulon-1 (RTN1) LDTP06633 9.99  LDD1988 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 7.94  LDD1988 
Sideroflexin-4 (SFXN4) LDTP07531 6.28  LDD1988 
Sigma intracellular receptor 2 (TMEM97) LDTP06945 14.72  LDD1988 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 9.19  LDD1988 
Solute carrier family 35 member F2 (SLC35F2) LDTP17641 7.46  LDD1988 
Sorting and assembly machinery component 50 homolog (SAMM50) LDTP14084 5.70  LDD1988 
StAR-related lipid transfer protein 7, mitochondrial (STARD7) LDTP18459 5.78  LDD1988 
STARD3 N-terminal-like protein (STARD3NL) LDTP01671 6.28  LDD1988 
Surfeit locus protein 4 (SURF4) LDTP00592 5.82  LDD1988 
Tetraspanin-3 (TSPAN3) LDTP16813 10.85  LDD1988 
Translocating chain-associated membrane protein 1 (TRAM1) LDTP06426 9.32  LDD1988 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 14.62  LDD1988 
Transmembrane 9 superfamily member 3 (TM9SF3) LDTP18448 9.19  LDD1988 
Transmembrane protein 126A (TMEM126A) LDTP15948 17.88  LDD1988 
Transmembrane protein 160 (TMEM160) LDTP20002 16.34  LDD1988 
Transmembrane protein 199 (TMEM199) LDTP08881 13.27  LDD1988 
Transmembrane protein 230 (TMEM230) LDTP10065 7.62  LDD1988 
Transmembrane protein 87A (TMEM87A) LDTP15481 11.08  LDD1988 
V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) LDTP09968 10.48  LDD1988 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 6.23  LDD1988 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 9.71  LDD1988 
Voltage-dependent anion-selective channel protein 3 (VDAC3) LDTP13824 6.02  LDD1988 
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⏷ Show the Full List of 63 Transporter and channel
1 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
DNA polymerase epsilon subunit 4 (POLE4) LDTP12471 6.68  LDD1988 
------------------------------------------------------------------------------------
63 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Alpha-internexin (INA) LDTP06527 6.28  LDD1988 
Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) LDTP08364 7.94  LDD1988 
Apolipoprotein L2 (APOL2) LDTP11076 13.18  LDD1988 
Bcl-2-like protein 13 (BCL2L13) LDTP11389 5.74  LDD1988 
Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) LDTP13755 9.45  LDD1988 
Calumenin (CALU) LDTP00887 7.16  LDD1988 
Clustered mitochondria protein homolog (CLUH) LDTP14486 10.27  LDD1988 
Cold-inducible RNA-binding protein (CIRBP) LDTP06022 6.28  LDD1988 
Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) LDTP13902 14.32  LDD1988 
Diablo IAP-binding mitochondrial protein (DIABLO) LDTP12468 7.46  LDD1988 
Dynactin subunit 3 (DCTN3) LDTP01359 9.13  LDD1988 
Dynactin subunit 4 (DCTN4) LDTP13402 16.00  LDD1988 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 7.21  LDD1988 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 10.41  LDD1988 
Growth factor receptor-bound protein 2 (GRB2) LDTP05035 5.62  LDD1988 
HEAT repeat-containing protein 1 (HEATR1) LDTP11948 7.46  LDD1988 
Heterogeneous nuclear ribonucleoprotein A/B (HNRNPAB) LDTP10962 6.23  LDD1988 
Heterogeneous nuclear ribonucleoprotein D-like (HNRNPDL) LDTP00500 5.74  LDD1988 
Heterogeneous nuclear ribonucleoprotein H (HNRNPH1) LDTP03615 5.82  LDD1988 
Heterogeneous nuclear ribonucleoprotein H3 (HNRNPH3) LDTP03614 6.87  LDD1988 
Heterogeneous nuclear ribonucleoprotein K (HNRNPK) LDTP04952 5.62  LDD1988 
Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP) LDTP00991 5.78  LDD1988 
Interleukin enhancer-binding factor 2 (ILF2) LDTP05688 7.16  LDD1988 
Intron Large complex component GCFC2 (GCFC2) LDTP02841 14.22  LDD1988 
Keratin, type I cytoskeletal 18 (KRT18) LDTP02124 6.28  LDD1988 
Keratinocyte-associated transmembrane protein 2 (KCT2) LDTP19780 12.38  LDD1988 
Large ribosomal subunit protein mL40 (MRPL40) LDTP12395 6.19  LDD1988 
MAGUK p55 subfamily member 2 (MPP2) LDTP06068 5.82  LDD1988 
MICOS complex subunit MIC13 (MICOS13) LDTP07274 5.94  LDD1988 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 44.63  LDD1988 
Mitochondrial import receptor subunit TOM5 homolog (TOMM5) LDTP15420 12.21  LDD1988 
Negative elongation factor C/D (NELFCD) LDTP08594 8.06  LDD1988 
Neudesin (NENF) LDTP13630 8.88  LDD1988 
Neuferricin (CYB5D2) LDTP15623 8.88  LDD1988 
NLR family member X1 (NLRX1) LDTP08248 5.94  LDD1988 
Nucleobindin-2 (NUCB2) LDTP05160 12.04  LDD1988 
Nucleosome assembly protein 1-like 1 (NAP1L1) LDTP04684 5.82  LDD1988 
OCIA domain-containing protein 1 (OCIAD1) LDTP12755 6.15  LDD1988 
Origin recognition complex subunit 3 (ORC3) LDTP13104 5.94  LDD1988 
Protein CEBPZOS (CEBPZOS) LDTP18883 7.73  LDD1988 
Protein CutA (CUTA) LDTP01075 7.21  LDD1988 
Protein FAM114A2 (FAM114A2) LDTP16080 6.41  LDD1988 
Protein FAM136A (FAM136A) LDTP18132 5.66  LDD1988 
Protein FAM162A (FAM162A) LDTP10056 6.54  LDD1988 
Protein unc-119 homolog A (UNC119) LDTP05868 11.39  LDD1988 
Protein unc-119 homolog B (UNC119B) LDTP00105 13.27  LDD1988 
Reticulocalbin-2 (RCN2) LDTP06097 8.51  LDD1988 
Reticulon-3 (RTN3) LDTP01522 15.67  LDD1988 
Reticulon-4 (RTN4) LDTP12412 9.85  LDD1988 
RNA-binding protein FUS (FUS) LDTP03775 7.11  LDD1988 
Synaptic vesicle glycoprotein 2A (SV2A) LDTP07934 6.45  LDD1988 
Synaptogyrin-2 (SYNGR2) LDTP00859 6.87  LDD1988 
Syntaxin-18 (STX18) LDTP13086 9.51  LDD1988 
TATA-binding protein-associated factor 2N (TAF15) LDTP09881 7.11  LDD1988 
Testis-expressed protein 264 (TEX264) LDTP14259 8.51  LDD1988 
Tetraspanin-6 (TSPAN6) LDTP14458 15.24  LDD1988 
Tetratricopeptide repeat protein 19, mitochondrial (TTC19) LDTP07368 6.02  LDD1988 
TraB domain-containing protein (TRABD) LDTP19963 10.34  LDD1988 
Trans-Golgi network integral membrane protein 2 (TGOLN2) LDTP00779 11.39  LDD1988 
Translation initiation factor eIF2B subunit delta (EIF2B4) LDTP13318 6.15  LDD1988 
Translation initiation factor eIF2B subunit gamma (EIF2B3) LDTP12476 6.41  LDD1988 
Tudor and KH domain-containing protein (TDRKH) LDTP13922 5.66  LDD1988 
Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) LDTP15067 17.63  LDD1988 
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⏷ Show the Full List of 63 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD1988 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587