Details of the Target

General Information of Target

Target ID LDTP10329
Target Name Ceramide synthase 2 (CERS2)
Gene Name CERS2
Gene ID 29956
Synonyms
LASS2; TMSG1; Ceramide synthase 2; CerS2; LAG1 longevity assurance homolog 2; SP260; Sphingosine N-acyltransferase CERS2; EC 2.3.1.24; Tumor metastasis-suppressor gene 1 protein; Very-long-chain ceramide synthase CERS2; EC 2.3.1.297
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLRREARLRREYLYRKAREEAQRSAQERKERLRRALEENRLIPTELRREALALQGSLEFD
DAGGEGVTSHVDDEYRWAGVEDPKVMITTSRDPSSRLKMFAKELKLVFPGAQRMNRGRHE
VGALVRACKANGVTDLLVVHEHRGTPVGLIVSHLPFGPTAYFTLCNVVMRHDIPDLGTMS
EAKPHLITHGFSSRLGKRVSDILRYLFPVPKDDSHRVITFANQDDYISFRHHVYKKTDHR
NVELTEVGPRFELKLYMIRLGTLEQEATADVEWRWHPYTNTARKRVFLSTE
Target Bioclass
Enzyme
Subcellular location
Endoplasmic reticulum membrane
Function
Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very-long-chain fatty acyl-CoA (chain length C22-C27). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively. Plays a non-redundant role in the synthesis of ceramides with very-long-chain fatty acids in kidney, liver and brain. Regulates the abundance of myelin-specific sphingolipids galactosylceramide and sulfatide that affects myelin sheath architecture and motor neuron functions.
Uniprot ID
Q96G23
Ensemble ID
ENST00000271688.10
HGNC ID
HGNC:14076

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
CHEMBL5175495
 Probe Info  		5.81  LDD0196  [1]
CY4
 Probe Info  		100.00  LDD0244  [2]
TH211
 Probe Info  		Y88(9.41)  LDD0260  [3]
STPyne
 Probe Info  		K131(10.00); K93(1.98)  LDD0277  [4]
Jackson_14
 Probe Info  		2.33  LDD0123  [5]
ATP probe
 Probe Info  		N.A.  LDD0035  [6]
Acrolein
 Probe Info  		N.A.  LDD0217  [7]
AOyne
 Probe Info  		14.50  LDD0443  [8]
PAL-AfBPP Probe
Click To Hide/Show 159 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C001
 Probe Info  		5.58  LDD1711  [9]
C003
 Probe Info  		14.93  LDD1713  [9]
C004
 Probe Info  		8.06  LDD1714  [9]
C007
 Probe Info  		6.45  LDD1716  [9]
C010
 Probe Info  		7.67  LDD1719  [9]
C011
 Probe Info  		7.89  LDD1720  [9]
C017
 Probe Info  		9.92  LDD1725  [9]
C022
 Probe Info  		14.83  LDD1728  [9]
C027
 Probe Info  		11.71  LDD1733  [9]
C040
 Probe Info  		30.91  LDD1740  [9]
C041
 Probe Info  		11.47  LDD1741  [9]
C049
 Probe Info  		5.39  LDD1747  [9]
C053
 Probe Info  		5.03  LDD1751  [9]
C055
 Probe Info  		35.75  LDD1752  [9]
C056
 Probe Info  		51.27  LDD1753  [9]
C063
 Probe Info  		17.03  LDD1760  [9]
C064
 Probe Info  		9.19  LDD1761  [9]
C070
 Probe Info  		13.45  LDD1766  [9]
C071
 Probe Info  		8.11  LDD1767  [9]
C072
 Probe Info  		8.06  LDD1768  [9]
C082
 Probe Info  		6.06  LDD1774  [9]
C085
 Probe Info  		7.01  LDD1777  [9]
C087
 Probe Info  		9.25  LDD1779  [9]
C091
 Probe Info  		33.59  LDD1782  [9]
C092
 Probe Info  		55.33  LDD1783  [9]
C094
 Probe Info  		66.72  LDD1785  [9]
C095
 Probe Info  		5.98  LDD1786  [9]
C100
 Probe Info  		5.78  LDD1789  [9]
C106
 Probe Info  		31.12  LDD1793  [9]
C107
 Probe Info  		11.31  LDD1794  [9]
C108
 Probe Info  		14.12  LDD1795  [9]
C112
 Probe Info  		41.64  LDD1799  [9]
C130
 Probe Info  		15.03  LDD1812  [9]
C131
 Probe Info  		5.28  LDD1813  [9]
C134
 Probe Info  		40.22  LDD1816  [9]
C135
 Probe Info  		15.56  LDD1817  [9]
C139
 Probe Info  		14.32  LDD1821  [9]
C140
 Probe Info  		9.92  LDD1822  [9]
C141
 Probe Info  		18.13  LDD1823  [9]
C143
 Probe Info  		24.93  LDD1825  [9]
C145
 Probe Info  		15.24  LDD1827  [9]
C147
 Probe Info  		11.88  LDD1829  [9]
C153
 Probe Info  		28.84  LDD1834  [9]
C158
 Probe Info  		15.78  LDD1838  [9]
C159
 Probe Info  		11.08  LDD1839  [9]
C160
 Probe Info  		20.11  LDD1840  [9]
C161
 Probe Info  		25.46  LDD1841  [9]
C163
 Probe Info  		9.71  LDD1843  [9]
C165
 Probe Info  		22.94  LDD1845  [9]
C166
 Probe Info  		12.55  LDD1846  [9]
C169
 Probe Info  		26.54  LDD1849  [9]
C170
 Probe Info  		9.58  LDD1850  [9]
C177
 Probe Info  		7.73  LDD1856  [9]
C178
 Probe Info  		16.11  LDD1857  [9]
C183
 Probe Info  		5.62  LDD1861  [9]
C186
 Probe Info  		12.82  LDD1864  [9]
C187
 Probe Info  		37.01  LDD1865  [9]
C191
 Probe Info  		9.85  LDD1868  [9]
C193
 Probe Info  		5.62  LDD1869  [9]
C194
 Probe Info  		21.56  LDD1870  [9]
C196
 Probe Info  		15.45  LDD1872  [9]
C198
 Probe Info  		18.64  LDD1874  [9]
C201
 Probe Info  		42.81  LDD1877  [9]
C206
 Probe Info  		29.86  LDD1881  [9]
C210
 Probe Info  		83.29  LDD1884  [9]
C212
 Probe Info  		7.26  LDD1886  [9]
C218
 Probe Info  		17.51  LDD1892  [9]
C220
 Probe Info  		25.63  LDD1894  [9]
C222
 Probe Info  		7.41  LDD1896  [9]
C225
 Probe Info  		7.41  LDD1898  [9]
C226
 Probe Info  		14.22  LDD1899  [9]
C228
 Probe Info  		36.76  LDD1901  [9]
C229
 Probe Info  		7.01  LDD1902  [9]
C231
 Probe Info  		19.70  LDD1904  [9]
C232
 Probe Info  		57.28  LDD1905  [9]
C233
 Probe Info  		14.12  LDD1906  [9]
C234
 Probe Info  		8.28  LDD1907  [9]
C235
 Probe Info  		35.02  LDD1908  [9]
C238
 Probe Info  		20.68  LDD1911  [9]
C239
 Probe Info  		15.56  LDD1912  [9]
C240
 Probe Info  		11.88  LDD1913  [9]
C243
 Probe Info  		18.77  LDD1916  [9]
C246
 Probe Info  		21.86  LDD1919  [9]
C249
 Probe Info  		12.73  LDD1922  [9]
C251
 Probe Info  		56.89  LDD1924  [9]
C252
 Probe Info  		32.67  LDD1925  [9]
C256
 Probe Info  		6.45  LDD1929  [9]
C270
 Probe Info  		7.73  LDD1940  [9]
C273
 Probe Info  		7.89  LDD1943  [9]
C275
 Probe Info  		6.82  LDD1945  [9]
C277
 Probe Info  		22.16  LDD1947  [9]
C278
 Probe Info  		54.95  LDD1948  [9]
C280
 Probe Info  		9.92  LDD1950  [9]
C282
 Probe Info  		37.01  LDD1952  [9]
C284
 Probe Info  		24.08  LDD1954  [9]
C285
 Probe Info  		25.28  LDD1955  [9]
C287
 Probe Info  		13.36  LDD1957  [9]
C288
 Probe Info  		9.58  LDD1958  [9]
C289
 Probe Info  		43.11  LDD1959  [9]
C290
 Probe Info  		9.06  LDD1960  [9]
C293
 Probe Info  		28.05  LDD1963  [9]
C296
 Probe Info  		19.16  LDD1966  [9]
C299
 Probe Info  		12.38  LDD1968  [9]
C303
 Probe Info  		7.41  LDD1972  [9]
C305
 Probe Info  		11.71  LDD1974  [9]
C310
 Probe Info  		22.01  LDD1977  [9]
C313
 Probe Info  		13.74  LDD1980  [9]
C314
 Probe Info  		13.93  LDD1981  [9]
C317
 Probe Info  		7.57  LDD1983  [9]
C320
 Probe Info  		6.50  LDD1986  [9]
C322
 Probe Info  		7.26  LDD1988  [9]
C326
 Probe Info  		10.85  LDD1990  [9]
C327
 Probe Info  		6.96  LDD1991  [9]
C333
 Probe Info  		4.92  LDD1996  [9]
C338
 Probe Info  		29.45  LDD2001  [9]
C339
 Probe Info  		21.41  LDD2002  [9]
C343
 Probe Info  		13.83  LDD2005  [9]
C346
 Probe Info  		15.78  LDD2007  [9]
C348
 Probe Info  		26.91  LDD2009  [9]
C349
 Probe Info  		40.79  LDD2010  [9]
C350
 Probe Info  		61.82  LDD2011  [9]
C353
 Probe Info  		10.27  LDD2014  [9]
C354
 Probe Info  		13.55  LDD2015  [9]
C355
 Probe Info  		25.46  LDD2016  [9]
C361
 Probe Info  		19.84  LDD2022  [9]
C362
 Probe Info  		99.73  LDD2023  [9]
C363
 Probe Info  		39.40  LDD2024  [9]
C364
 Probe Info  		35.51  LDD2025  [9]
C366
 Probe Info  		17.15  LDD2027  [9]
C367
 Probe Info  		7.94  LDD2028  [9]
C373
 Probe Info  		5.98  LDD2033  [9]
C376
 Probe Info  		18.90  LDD2036  [9]
C378
 Probe Info  		10.27  LDD2037  [9]
C380
 Probe Info  		7.36  LDD2039  [9]
C382
 Probe Info  		26.91  LDD2041  [9]
C383
 Probe Info  		33.36  LDD2042  [9]
C386
 Probe Info  		9.19  LDD2045  [9]
C388
 Probe Info  		99.73  LDD2047  [9]
C399
 Probe Info  		7.94  LDD2058  [9]
C403
 Probe Info  		20.53  LDD2061  [9]
C407
 Probe Info  		23.43  LDD2064  [9]
C413
 Probe Info  		11.88  LDD2069  [9]
C420
 Probe Info  		15.03  LDD2075  [9]
C424
 Probe Info  		7.78  LDD2079  [9]
C426
 Probe Info  		28.05  LDD2081  [9]
C428
 Probe Info  		7.78  LDD2083  [9]
C429
 Probe Info  		23.92  LDD2084  [9]
C431
 Probe Info  		30.48  LDD2086  [9]
C433
 Probe Info  		5.86  LDD2088  [9]
FFF probe11
 Probe Info  		20.00  LDD0471  [10]
FFF probe13
 Probe Info  		20.00  LDD0475  [10]
FFF probe14
 Probe Info  		13.46  LDD0477  [10]
FFF probe2
 Probe Info  		20.00  LDD0463  [10]
FFF probe3
 Probe Info  		20.00  LDD0464  [10]
JN0003
 Probe Info  		20.00  LDD0469  [10]
VE-P
 Probe Info  		N.A.  LDD0396  [11]
A-DA
 Probe Info  		2.52  LDD0145  [12]
AEA-DA
 Probe Info  		4.99  LDD0333  [13]
OEA-DA
 Probe Info  		19.73  LDD0046  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [7]
 LDCM0107  IAA HeLa N.A.  LDD0221  [7]
 LDCM0109  NEM HeLa N.A.  LDD0223  [7]
 LDCM0016  Ranjitkar_cp1 MDA-MB-231 2.33  LDD0123  [5]
 LDCM0084  Ro 48-8071 A-549 2.52  LDD0145  [12]
 LDCM0136  SR-4559 HEK-293T 4.99  LDD0333  [13]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Heat shock protein HSP 90-alpha (HSP90AA1) Heat shock protein 90 family P07900
V-type proton ATPase 16 kDa proteolipid subunit c (ATP6V0C) V-ATPase proteolipid subunit family P27449
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Solute carrier family 22 member 1 (SLC22A1) Organic cation transporter (TC 2.A.1.19) family O15245
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nuclear factor erythroid 2-related factor 2 (NFE2L2) BZIP family Q16236
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein YIF1A (YIF1A) YIF1 family O95070
Immediate early response 3-interacting protein 1 (IER3IP1) YOS1 family Q9Y5U9
Asialoglycoprotein receptor 1 (ASGR1) . P07306
Asialoglycoprotein receptor 2 (ASGR2) . P07307

References

1 Charting the Chemical Space of Acrylamide-Based Inhibitors of zDHHC20. ACS Med Chem Lett. 2022 Sep 26;13(10):1648-1654. doi: 10.1021/acsmedchemlett.2c00336. eCollection 2022 Oct 13.
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
5 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
6 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
11 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
12 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.
13 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570