Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C049  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 45 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP00697  Cytochrome b5 type B (CYB5B) 29.9
 LDTP06477  Methylsterol monooxygenase 1 (MSMO1) 19.7
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 18.8
 LDTP02359  Heme oxygenase 1 (HMOX1) 17.5
 LDTP03540  Heme oxygenase 2 (HMOX2) 16.9
 LDTP13189  Cytochrome b-c1 complex subunit 9 (UQCR10) 16.6
 LDTP08728  Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) 16.3
 LDTP00486  Cytochrome b-c1 complex subunit 8 (UQCRQ) 12.0
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 11.2
 LDTP06787  Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) 9.1
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 8.9
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 8.2
 LDTP10032  7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) 8.0
 LDTP11874  Thioredoxin-related transmembrane protein 1 (TMX1) 8.0
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 7.5
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 7.4
 LDTP11127  ADP-dependent glucokinase (ADPGK) 7.2
 LDTP03394  Ceramide synthase 1 (CERS1) 7.1
 LDTP01613  Sphingosine-1-phosphate lyase 1 (SGPL1) 7.0
 LDTP08385  NADH dehydrogenase 1 alpha subcomplex subunit 11 (NDUFA11) 6.8
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 6.8
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 6.6
 LDTP00907  Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) 6.5
 LDTP12873  CDGSH iron-sulfur domain-containing protein 1 (CISD1) 6.3
 LDTP03145  Catechol O-methyltransferase (COMT) 6.1
 LDTP06325  3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP) 6.0
 LDTP14276  NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9) 5.9
 LDTP12959  NADH dehydrogenase 1 alpha subcomplex subunit 13 (NDUFA13) 5.9
 LDTP10020  Ras-related protein Rab-24 (RAB24) 5.8
 LDTP09396  Minor histocompatibility antigen H13 (HM13) 5.7
 LDTP10550  Dehydrogenase/reductase SDR family member 1 (DHRS1) 5.7
 LDTP15328  Dolichyldiphosphatase 1 (DOLPP1) 5.6
 LDTP02005  Lysosomal acid glucosylceramidase (GBA1) 5.6
 LDTP18087  Dephospho-CoA kinase domain-containing protein (DCAKD) 5.5
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 5.4
 LDTP10329  Ceramide synthase 2 (CERS2) 5.4
 LDTP04051  Nicotinamide phosphoribosyltransferase (NAMPT) 5.4
 LDTP16032  Retinoid-inducible serine carboxypeptidase (SCPEP1) 5.2
 LDTP02896  Sphingomyelin phosphodiesterase (SMPD1) 5.1
 LDTP07620  Peroxisomal N(1)-acetyl-spermine/spermidine oxidase (PAOX) 5.1
 LDTP11199  DCN1-like protein 5 (DCUN1D5) 5.1
 LDTP09061  Retinol dehydrogenase 13 (RDH13) 5.1
 LDTP17410  Cytochrome P450 20A1 (CYP20A1) 5.0
 LDTP01389  Delta(14)-sterol reductase TM7SF2 (TM7SF2) 5.0
 LDTP10182  TLC domain-containing protein 1 (TLCD1) 5.0
Transporter and channel
Click to Show/Hide to Show 43 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 38.6
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 30.1
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 24.9
 LDTP08878  ER membrane protein complex subunit 5 (MMGT1) 22.3
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 18.5
 LDTP15948  Transmembrane protein 126A (TMEM126A) 16.6
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 15.8
 LDTP16069  Peroxisomal membrane protein 2 (PXMP2) 14.9
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 13.9
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 11.9
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 11.4
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 10.6
 LDTP06275  Lysosomal-associated transmembrane protein 4A (LAPTM4A) 10.2
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 10.1
 LDTP14235  Mitochondrial carrier homolog 2 (MTCH2) 9.8
 LDTP03546  Translocator protein (TSPO) 8.7
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 8.5
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 8.1
 LDTP01745  Putative lipid scramblase CLPTM1 (CLPTM1) 7.8
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 7.7
 LDTP06308  Mitochondrial inner membrane protein OXA1L (OXA1L) 7.6
 LDTP00405  Etoposide-induced protein 2.4 homolog (EI24) 7.5
 LDTP09180  Zinc transporter 7 (SLC30A7) 7.5
 LDTP15648  BRI3-binding protein (BRI3BP) 7.1
 LDTP09328  Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) 7.1
 LDTP08881  Transmembrane protein 199 (TMEM199) 7.1
 LDTP01542  Golgi SNAP receptor complex member 1 (GOSR1) 7.0
 LDTP11844  Protein spinster homolog 1 (SPNS1) 6.9
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 6.8
 LDTP04787  Transmembrane protein 33 (TMEM33) 6.7
 LDTP11162  Solute carrier family 25 member 33 (SLC25A33) 6.6
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 6.6
 LDTP13824  Voltage-dependent anion-selective channel protein 3 (VDAC3) 6.2
 LDTP02645  Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1) 5.8
 LDTP00592  Surfeit locus protein 4 (SURF4) 5.8
 LDTP10065  Transmembrane protein 230 (TMEM230) 5.8
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 5.7
 LDTP11213  Nucleoporin NDC1 (NDC1) 5.7
 LDTP10499  Lipid scramblase CLPTM1L (CLPTM1L) 5.4
 LDTP06945  Sigma intracellular receptor 2 (TMEM97) 5.4
 LDTP00477  Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) 5.3
 LDTP17362  Magnesium transporter NIPA3 (NIPAL1) 5.1
 LDTP06633  Reticulon-1 (RTN1) 4.9
Other
Click to Show/Hide to Show 37 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP03678  ER lumen protein-retaining receptor 2 (KDELR2) 49.2
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 28.8
 LDTP00850  ER lumen protein-retaining receptor 3 (KDELR3) 20.4
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 17.6
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 15.1
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 14.4
 LDTP15420  Mitochondrial import receptor subunit TOM5 homolog (TOMM5) 14.2
 LDTP11076  Apolipoprotein L2 (APOL2) 12.6
 LDTP01522  Reticulon-3 (RTN3) 12.1
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 11.9
 LDTP05160  Nucleobindin-2 (NUCB2) 11.3
 LDTP13797  HIG1 domain family member 1A, mitochondrial (HIGD1A) 10.9
 LDTP11277  Tubulin beta-2B chain (TUBB2B) 10.0
 LDTP12412  Reticulon-4 (RTN4) 9.9
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 9.8
 LDTP05375  Nucleobindin-1 (NUCB1) 9.7
 LDTP18883  Protein CEBPZOS (CEBPZOS) 8.7
 LDTP02205  Tubulin beta chain (TUBB) 8.5
 LDTP10056  Protein FAM162A (FAM162A) 8.2
 LDTP02037  Tubulin beta-4A chain (TUBB4A) 8.1
 LDTP16323  Signal recognition particle receptor subunit beta (SRPRB) 8.0
 LDTP05998  Tubulin beta-2A chain (TUBB2A) 7.7
 LDTP18373  Thioredoxin-related transmembrane protein 4 (TMX4) 7.5
 LDTP14458  Tetraspanin-6 (TSPAN6) 7.2
 LDTP16006  Optic atrophy 3 protein (OPA3) 7.0
 LDTP13086  Syntaxin-18 (STX18) 7.0
 LDTP13922  Tudor and KH domain-containing protein (TDRKH) 6.7
 LDTP08224  Protein LYRIC (MTDH) 6.6
 LDTP05076  Tubulin beta-4B chain (TUBB4B) 6.6
 LDTP08904  Protein jagunal homolog 1 (JAGN1) 6.0
 LDTP16080  Protein FAM114A2 (FAM114A2) 5.7
 LDTP03926  Centrin-2 (CETN2) 5.4
 LDTP11233  Tubulin beta-6 chain (TUBB6) 5.3
 LDTP04708  NHP2-like protein 1 (SNU13) 5.1
 LDTP13311  Poly(U)-binding-splicing factor PUF60 (PUF60) 5.1
 LDTP15634  Ubiquinol-cytochrome c reductase complex assembly factor 5 (UQCC5) 5.1
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 4.9

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587