General Information of Target

Target ID LDTP06426
Target Name Translocating chain-associated membrane protein 1 (TRAM1)
Gene Name TRAM1
Gene ID 23471
Synonyms
TRAM; Translocating chain-associated membrane protein 1; Protein TRAM1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAIRKKSTKSPPVLSHEFVLQNHADIVSCVAMVFLLGLMFEITAKASIIFVTLQYNVTLP
ATEEQATESVSLYYYGIKDLATVFFYMLVAIIIHAVIQEYMLDKINRRMHFSKTKHSKFN
ESGQLSAFYLFACVWGTFILISENYISDPTILWRAYPHNLMTFQMKFFYISQLAYWLHAF
PELYFQKTKKEDIPRQLVYIGLYLFHIAGAYLLNLNHLGLVLLVLHYFVEFLFHISRLFY
FSNEKYQKGFSLWAVLFVLGRLLTLILSVLTVGFGLARAENQKLDFSTGNFNVLAVRIAV
LASICVTQAFMMWKFINFQLRRWREHSAFQAPAVKKKPTVTKGRSSKKGTENGVNGTLTS
NVADSPRNKKEKSS
Target Bioclass
Transporter and channel
Family
TRAM family
Subcellular location
Endoplasmic reticulum membrane
Function
Involved in the translocation of nascent protein chains into or through the endoplasmic reticulum (ER) membrane by facilitating the proper chain positioning at the SEC61 channel. Regulates the exposure of nascent secretory protein chain to the cytosol during translocation into the ER. May affect the phospholipid bilayer in the vicinity of the lateral gate of the SEC61 channel, thereby facilitating ER protein transport. Intimately associates with transmembrane (TM) domain of nascent membrane proteins during the entire integration process into the ER membrane. Associates with the second TM domain of G-protein-coupled receptor opsin/OPSD nascent chain in the ER membrane, which may facilitate its integration into the membrane. Under conditions of ER stress, participates in the disposal of misfolded ER membrane proteins during the unfolded protein response (UPR), an integrated stress response (ISR) pathway, by selectively retrotranslocating misfolded ER-membrane proteins from the ER into the cytosol where they are ubiquitinated and degraded by the proteasome.; (Microbial infection) In case of cytomegalovirus infection, participates in US2- and US11-mediated ER-to-cytosol retrotranslocation and subsequent degradation of major histocompatibility complex (MHC) class I heavy chains, thereby decreasing the immune detection by cytotoxic T-cells.
Uniprot ID
Q15629
Ensemble ID
ENST00000262213.7
HGNC ID
HGNC:20568

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info 
4.54  LDD0318  [1]
YN-1
 Probe Info 
100.00  LDD0444  [2]
STPyne
 Probe Info 
K113(4.39); K342(8.25)  LDD0277  [3]
Acrolein
 Probe Info 
N.A.  LDD0217  [4]
PAL-AfBPP Probe
Click To Hide/Show 165 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
PARPYnD
 Probe Info 
1.66  LDD0374  [5]
C001
 Probe Info 
5.66  LDD1711  [6]
C004
 Probe Info 
8.40  LDD1714  [6]
C007
 Probe Info 
4.99  LDD1716  [6]
C008
 Probe Info 
8.28  LDD1717  [6]
C010
 Probe Info 
12.64  LDD1719  [6]
C011
 Probe Info 
11.39  LDD1720  [6]
C017
 Probe Info 
7.36  LDD1725  [6]
C022
 Probe Info 
15.03  LDD1728  [6]
C039
 Probe Info 
10.13  LDD1739  [6]
C040
 Probe Info 
38.59  LDD1740  [6]
C041
 Probe Info 
15.56  LDD1741  [6]
C049
 Probe Info 
10.13  LDD1747  [6]
C055
 Probe Info 
33.13  LDD1752  [6]
C056
 Probe Info 
32.67  LDD1753  [6]
C059
 Probe Info 
5.74  LDD1756  [6]
C060
 Probe Info 
5.86  LDD1757  [6]
C063
 Probe Info 
37.01  LDD1760  [6]
C064
 Probe Info 
9.78  LDD1761  [6]
C067
 Probe Info 
11.16  LDD1763  [6]
C070
 Probe Info 
24.59  LDD1766  [6]
C071
 Probe Info 
10.27  LDD1767  [6]
C072
 Probe Info 
21.41  LDD1768  [6]
C082
 Probe Info 
7.57  LDD1774  [6]
C085
 Probe Info 
5.70  LDD1777  [6]
C087
 Probe Info 
9.19  LDD1779  [6]
C091
 Probe Info 
34.54  LDD1782  [6]
C092
 Probe Info 
44.94  LDD1783  [6]
C094
 Probe Info 
57.28  LDD1785  [6]
C106
 Probe Info 
30.27  LDD1793  [6]
C107
 Probe Info 
20.39  LDD1794  [6]
C108
 Probe Info 
24.93  LDD1795  [6]
C112
 Probe Info 
38.05  LDD1799  [6]
C129
 Probe Info 
10.20  LDD1811  [6]
C130
 Probe Info 
13.55  LDD1812  [6]
C134
 Probe Info 
35.02  LDD1816  [6]
C135
 Probe Info 
24.08  LDD1817  [6]
C139
 Probe Info 
22.32  LDD1821  [6]
C140
 Probe Info 
14.72  LDD1822  [6]
C141
 Probe Info 
25.63  LDD1823  [6]
C143
 Probe Info 
34.78  LDD1825  [6]
C147
 Probe Info 
11.63  LDD1829  [6]
C153
 Probe Info 
30.91  LDD1834  [6]
C159
 Probe Info 
20.25  LDD1839  [6]
C160
 Probe Info 
23.10  LDD1840  [6]
C161
 Probe Info 
34.30  LDD1841  [6]
C163
 Probe Info 
13.93  LDD1843  [6]
C165
 Probe Info 
13.00  LDD1845  [6]
C166
 Probe Info 
6.77  LDD1846  [6]
C169
 Probe Info 
69.07  LDD1849  [6]
C170
 Probe Info 
12.55  LDD1850  [6]
C173
 Probe Info 
12.04  LDD1853  [6]
C174
 Probe Info 
6.36  LDD1854  [6]
C178
 Probe Info 
18.38  LDD1857  [6]
C183
 Probe Info 
5.13  LDD1861  [6]
C186
 Probe Info 
11.96  LDD1864  [6]
C194
 Probe Info 
27.67  LDD1870  [6]
C196
 Probe Info 
29.24  LDD1872  [6]
C197
 Probe Info 
7.84  LDD1873  [6]
C198
 Probe Info 
27.86  LDD1874  [6]
C210
 Probe Info 
45.57  LDD1884  [6]
C211
 Probe Info 
23.10  LDD1885  [6]
C213
 Probe Info 
28.64  LDD1887  [6]
C214
 Probe Info 
6.59  LDD1888  [6]
C218
 Probe Info 
30.27  LDD1892  [6]
C219
 Probe Info 
16.34  LDD1893  [6]
C220
 Probe Info 
43.11  LDD1894  [6]
C222
 Probe Info 
6.06  LDD1896  [6]
C225
 Probe Info 
22.01  LDD1898  [6]
C226
 Probe Info 
20.97  LDD1899  [6]
C228
 Probe Info 
49.18  LDD1901  [6]
C229
 Probe Info 
15.89  LDD1902  [6]
C231
 Probe Info 
48.84  LDD1904  [6]
C232
 Probe Info 
88.03  LDD1905  [6]
C233
 Probe Info 
42.22  LDD1906  [6]
C234
 Probe Info 
25.63  LDD1907  [6]
C235
 Probe Info 
66.26  LDD1908  [6]
C238
 Probe Info 
26.17  LDD1911  [6]
C240
 Probe Info 
17.51  LDD1913  [6]
C243
 Probe Info 
31.78  LDD1916  [6]
C244
 Probe Info 
36.25  LDD1917  [6]
C246
 Probe Info 
43.11  LDD1919  [6]
C249
 Probe Info 
33.82  LDD1922  [6]
C252
 Probe Info 
14.12  LDD1925  [6]
C258
 Probe Info 
6.19  LDD1931  [6]
C260
 Probe Info 
6.28  LDD1932  [6]
C264
 Probe Info 
23.43  LDD1935  [6]
C265
 Probe Info 
17.51  LDD1936  [6]
C270
 Probe Info 
12.55  LDD1940  [6]
C273
 Probe Info 
10.06  LDD1943  [6]
C277
 Probe Info 
14.42  LDD1947  [6]
C278
 Probe Info 
54.19  LDD1948  [6]
C280
 Probe Info 
15.56  LDD1950  [6]
C282
 Probe Info 
45.57  LDD1952  [6]
C284
 Probe Info 
41.07  LDD1954  [6]
C285
 Probe Info 
35.75  LDD1955  [6]
C286
 Probe Info 
6.32  LDD1956  [6]
C287
 Probe Info 
25.99  LDD1957  [6]
C288
 Probe Info 
21.71  LDD1958  [6]
C289
 Probe Info 
34.30  LDD1959  [6]
C290
 Probe Info 
11.47  LDD1960  [6]
C293
 Probe Info 
29.65  LDD1963  [6]
C296
 Probe Info 
25.81  LDD1966  [6]
C299
 Probe Info 
20.97  LDD1968  [6]
C305
 Probe Info 
13.45  LDD1974  [6]
C307
 Probe Info 
8.63  LDD1975  [6]
C310
 Probe Info 
12.47  LDD1977  [6]
C313
 Probe Info 
19.03  LDD1980  [6]
C314
 Probe Info 
26.35  LDD1981  [6]
C317
 Probe Info 
6.77  LDD1983  [6]
C322
 Probe Info 
9.32  LDD1988  [6]
C326
 Probe Info 
13.93  LDD1990  [6]
C338
 Probe Info 
12.47  LDD2001  [6]
C339
 Probe Info 
18.25  LDD2002  [6]
C342
 Probe Info 
5.17  LDD2004  [6]
C343
 Probe Info 
36.00  LDD2005  [6]
C348
 Probe Info 
13.00  LDD2009  [6]
C349
 Probe Info 
19.56  LDD2010  [6]
C350
 Probe Info 
36.25  LDD2011  [6]
C353
 Probe Info 
7.21  LDD2014  [6]
C355
 Probe Info 
32.45  LDD2016  [6]
C356
 Probe Info 
16.80  LDD2017  [6]
C361
 Probe Info 
19.97  LDD2022  [6]
C362
 Probe Info 
88.03  LDD2023  [6]
C363
 Probe Info 
51.63  LDD2024  [6]
C364
 Probe Info 
46.85  LDD2025  [6]
C366
 Probe Info 
34.30  LDD2027  [6]
C367
 Probe Info 
17.15  LDD2028  [6]
C373
 Probe Info 
8.88  LDD2033  [6]
C376
 Probe Info 
11.16  LDD2036  [6]
C382
 Probe Info 
8.88  LDD2041  [6]
C383
 Probe Info 
20.97  LDD2042  [6]
C386
 Probe Info 
10.27  LDD2045  [6]
C387
 Probe Info 
5.17  LDD2046  [6]
C388
 Probe Info 
36.50  LDD2047  [6]
C390
 Probe Info 
24.25  LDD2049  [6]
C397
 Probe Info 
11.16  LDD2056  [6]
C399
 Probe Info 
16.11  LDD2058  [6]
C403
 Probe Info 
29.45  LDD2061  [6]
C405
 Probe Info 
8.22  LDD2063  [6]
C407
 Probe Info 
25.46  LDD2064  [6]
C408
 Probe Info 
6.15  LDD2065  [6]
C409
 Probe Info 
8.88  LDD2066  [6]
C419
 Probe Info 
18.13  LDD2074  [6]
C420
 Probe Info 
25.28  LDD2075  [6]
C421
 Probe Info 
9.19  LDD2076  [6]
C422
 Probe Info 
11.55  LDD2077  [6]
C423
 Probe Info 
10.27  LDD2078  [6]
C424
 Probe Info 
9.71  LDD2079  [6]
C426
 Probe Info 
14.72  LDD2081  [6]
C429
 Probe Info 
33.36  LDD2084  [6]
C431
 Probe Info 
44.94  LDD2086  [6]
C433
 Probe Info 
6.82  LDD2088  [6]
FFF probe11
 Probe Info 
20.00  LDD0471  [7]
FFF probe13
 Probe Info 
20.00  LDD0475  [7]
FFF probe14
 Probe Info 
20.00  LDD0477  [7]
FFF probe15
 Probe Info 
15.85  LDD0478  [7]
FFF probe2
 Probe Info 
20.00  LDD0463  [7]
FFF probe3
 Probe Info 
20.00  LDD0464  [7]
FFF probe4
 Probe Info 
18.74  LDD0466  [7]
FFF probe6
 Probe Info 
18.77  LDD0467  [7]
FFF probe9
 Probe Info 
20.00  LDD0470  [7]
JN0003
 Probe Info 
20.00  LDD0469  [7]
A-DA
 Probe Info 
5.25  LDD0145  [8]
OEA-DA
 Probe Info 
20.00  LDD0046  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [4]
 LDCM0185  Compound 17 HEK-293T 4.09  LDD0512  [7]
 LDCM0191  Compound 21 HEK-293T 8.20  LDD0508  [7]
 LDCM0190  Compound 34 HEK-293T 13.70  LDD0510  [7]
 LDCM0192  Compound 35 HEK-293T 9.71  LDD0509  [7]
 LDCM0193  Compound 36 HEK-293T 10.26  LDD0511  [7]
 LDCM0107  IAA HeLa N.A.  LDD0221  [4]
 LDCM0109  NEM HeLa N.A.  LDD0223  [4]
 LDCM0084  Ro 48-8071 A-549 5.25  LDD0145  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
B-cell receptor-associated protein 31 (BCAP31) BCAP29/BCAP31 family P51572
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Guanylin (GUCA2A) Guanylin family Q02747

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Structure-Guided Design and In-Cell Target Profiling of a Cell-Active Target Engagement Probe for PARP Inhibitors. ACS Chem Biol. 2020 Feb 21;15(2):325-333. doi: 10.1021/acschembio.9b00963. Epub 2020 Feb 10.
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
7 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
8 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.
9 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570