Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C322  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 78 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 27.9
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 24.1
 LDTP04247  Protein farnesyltransferase subunit beta (FNTB) 22.6
 LDTP00697  Cytochrome b5 type B (CYB5B) 22.3
 LDTP16040  Glyoxalase domain-containing protein 4 (GLOD4) 21.3
 LDTP00907  Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) 20.8
 LDTP11127  ADP-dependent glucokinase (ADPGK) 16.3
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 16.1
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 15.6
 LDTP12628  Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) 14.7
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 14.6
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 13.3
 LDTP03842  Squalene synthase (FDFT1) 12.3
 LDTP03540  Heme oxygenase 2 (HMOX2) 11.7
 LDTP16007  Lipid droplet-associated hydrolase (LDAH) 11.6
 LDTP13189  Cytochrome b-c1 complex subunit 9 (UQCR10) 10.8
 LDTP04051  Nicotinamide phosphoribosyltransferase (NAMPT) 10.8
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 10.4
 LDTP00486  Cytochrome b-c1 complex subunit 8 (UQCRQ) 10.4
 LDTP17410  Cytochrome P450 20A1 (CYP20A1) 10.4
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 10.4
 LDTP03787  Choline kinase alpha (CHKA) 9.6
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 9.2
 LDTP06325  3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP) 9.1
 LDTP15484  Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) 9.1
 LDTP06200  Delta(14)-sterol reductase LBR (LBR) 8.9
 LDTP12444  Xaa-Pro aminopeptidase 1 (XPNPEP1) 8.6
 LDTP04032  Glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2) 8.6
 LDTP03331  Proteasome subunit alpha type-2 (PSMA2) 8.6
 LDTP08385  NADH dehydrogenase 1 alpha subcomplex subunit 11 (NDUFA11) 8.2
 LDTP06787  Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) 8.0
 LDTP05862  NAD(P) transhydrogenase, mitochondrial (NNT) 7.9
 LDTP06901  Very-long-chain 3-oxoacyl-CoA reductase (HSD17B12) 7.9
 LDTP03257  Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) 7.7
 LDTP09396  Minor histocompatibility antigen H13 (HM13) 7.7
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 7.7
 LDTP08265  N-alpha-acetyltransferase 40 (NAA40) 7.6
 LDTP08455  Palmitoyltransferase ZDHHC13 (ZDHHC13) 7.6
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 7.6
 LDTP12868  Endoplasmic reticulum aminopeptidase 1 (ERAP1) 7.5
 LDTP04008  Endothelin-converting enzyme 1 (ECE1) 7.3
 LDTP10329  Ceramide synthase 2 (CERS2) 7.3
 LDTP00589  Peroxisomal acyl-coenzyme A oxidase 3 (ACOX3) 7.3
 LDTP08017  Endoplasmic reticulum metallopeptidase 1 (ERMP1) 7.2
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 7.0
 LDTP12363  Lysophosphatidic acid phosphatase type 6 (ACP6) 7.0
 LDTP12013  dCTP pyrophosphatase 1 (DCTPP1) 6.9
 LDTP11321  Acetyl-CoA acetyltransferase, cytosolic (ACAT2) 6.9
 LDTP04581  Holocytochrome c-type synthase (HCCS) 6.8
 LDTP09666  Reticulon-4-interacting protein 1, mitochondrial (RTN4IP1) 6.8
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 6.7
 LDTP14276  NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9) 6.6
 LDTP00988  Protein O-GlcNAcase (OGA) 6.5
 LDTP16070  Epimerase family protein SDR39U1 (SDR39U1) 6.5
 LDTP03417  Proteasome subunit beta type-4 (PSMB4) 6.4
 LDTP01329  Lathosterol oxidase (SC5D) 6.3
 LDTP11437  Peroxisomal trans-2-enoyl-CoA reductase (PECR) 6.3
 LDTP03001  E3 ubiquitin-protein ligase TRIM21 (TRIM21) 6.3
 LDTP12586  Phenylalanine--tRNA ligase beta subunit (FARSB) 6.3
 LDTP06333  Serum paraoxonase/arylesterase 2 (PON2) 6.3
 LDTP09395  Signal peptide peptidase-like 2A (SPPL2A) 6.3
 LDTP10032  7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) 6.1
 LDTP14030  V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) 6.1
 LDTP11874  Thioredoxin-related transmembrane protein 1 (TMX1) 6.1
 LDTP03671  Multidrug resistance-associated protein 1 (ABCC1) 6.0
 LDTP02679  Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) 6.0
 LDTP10386  ERO1-like protein alpha (ERO1A) 5.9
 LDTP08598  NAD-dependent protein deacetylase sirtuin-2 (SIRT2) 5.9
 LDTP07003  Presequence protease, mitochondrial (PITRM1) 5.9
 LDTP03518  Enoyl-CoA hydratase, mitochondrial (ECHS1) 5.9
 LDTP06477  Methylsterol monooxygenase 1 (MSMO1) 5.8
 LDTP06307  Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) 5.8
 LDTP06750  Prolyl 3-hydroxylase 1 (P3H1) 5.8
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 5.7
 LDTP03316  DNA replication licensing factor MCM3 (MCM3) 5.7
 LDTP05652  Aspartyl/asparaginyl beta-hydroxylase (ASPH) 5.7
 LDTP13220  Phosphatidylserine decarboxylase proenzyme, mitochondrial (PISD) 5.7
 LDTP05588  RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2) 5.7
Transporter and channel
Click to Show/Hide to Show 63 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 60.5
 LDTP01804  ATP synthase subunit a (MT-ATP6) 57.3
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 45.6
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 23.6
 LDTP05900  Metaxin-1 (MTX1) 18.9
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 18.4
 LDTP15948  Transmembrane protein 126A (TMEM126A) 17.9
 LDTP01601  Activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1) 17.8
 LDTP20002  Transmembrane protein 160 (TMEM160) 16.3
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 16.2
 LDTP11095  Mitochondrial 2-oxodicarboxylate carrier (SLC25A21) 16.0
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 14.9
 LDTP06945  Sigma intracellular receptor 2 (TMEM97) 14.7
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 14.6
 LDTP14099  Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) 14.5
 LDTP08881  Transmembrane protein 199 (TMEM199) 13.3
 LDTP10036  BOS complex subunit NCLN (NCLN) 12.3
 LDTP06308  Mitochondrial inner membrane protein OXA1L (OXA1L) 12.0
 LDTP11245  Derlin-1 (DERL1) 11.2
 LDTP15481  Transmembrane protein 87A (TMEM87A) 11.1
 LDTP07236  Protein GPR107 (GPR107) 11.0
 LDTP16813  Tetraspanin-3 (TSPAN3) 10.9
 LDTP09968  V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) 10.5
 LDTP08878  ER membrane protein complex subunit 5 (MMGT1) 10.1
 LDTP06633  Reticulon-1 (RTN1) 10.0
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 9.7
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 9.6
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 9.3
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 9.2
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 9.2
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 8.7
 LDTP15648  BRI3-binding protein (BRI3BP) 8.3
 LDTP00235  Membrane-associated progesterone receptor component 1 (PGRMC1) 8.2
 LDTP01217  Metaxin-2 (MTX2) 8.1
 LDTP04597  Methylosome subunit pICln (CLNS1A) 7.9
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 7.9
 LDTP10513  Exocyst complex component 2 (EXOC2) 7.9
 LDTP10065  Transmembrane protein 230 (TMEM230) 7.6
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 7.5
 LDTP17641  Solute carrier family 35 member F2 (SLC35F2) 7.5
 LDTP01745  Putative lipid scramblase CLPTM1 (CLPTM1) 7.4
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 6.9
 LDTP14068  Cysteine protease ATG4B (ATG4B) 6.7
 LDTP02645  Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1) 6.7
 LDTP11213  Nucleoporin NDC1 (NDC1) 6.6
 LDTP03768  Alpha-actinin-2 (ACTN2) 6.5
 LDTP09328  Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) 6.3
 LDTP09203  Nucleoporin Nup37 (NUP37) 6.3
 LDTP07531  Sideroflexin-4 (SFXN4) 6.3
 LDTP01671  STARD3 N-terminal-like protein (STARD3NL) 6.3
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 6.2
 LDTP10059  PAT complex subunit CCDC47 (CCDC47) 6.1
 LDTP10499  Lipid scramblase CLPTM1L (CLPTM1L) 6.1
 LDTP09294  Proton-coupled zinc antiporter SLC30A5 (SLC30A5) 6.0
 LDTP13824  Voltage-dependent anion-selective channel protein 3 (VDAC3) 6.0
 LDTP03952  Reduced folate transporter (SLC19A1) 5.9
 LDTP11607  Exportin-4 (XPO4) 5.8
 LDTP00010  Intraflagellar transport protein 56 (IFT56) 5.8
 LDTP00592  Surfeit locus protein 4 (SURF4) 5.8
 LDTP18459  StAR-related lipid transfer protein 7, mitochondrial (STARD7) 5.8
 LDTP00865  Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) 5.7
 LDTP14084  Sorting and assembly machinery component 50 homolog (SAMM50) 5.7
 LDTP14200  Mitochondrial ornithine transporter 1 (SLC25A15) 5.6
Transcription factor
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12471  DNA polymerase epsilon subunit 4 (POLE4) 6.7
Other
Click to Show/Hide to Show 63 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 44.6
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 17.6
 LDTP13402  Dynactin subunit 4 (DCTN4) 16.0
 LDTP01522  Reticulon-3 (RTN3) 15.7
 LDTP14458  Tetraspanin-6 (TSPAN6) 15.2
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 14.3
 LDTP02841  Intron Large complex component GCFC2 (GCFC2) 14.2
 LDTP00105  Protein unc-119 homolog B (UNC119B) 13.3
 LDTP11076  Apolipoprotein L2 (APOL2) 13.2
 LDTP19780  Keratinocyte-associated transmembrane protein 2 (KCT2) 12.4
 LDTP15420  Mitochondrial import receptor subunit TOM5 homolog (TOMM5) 12.2
 LDTP05160  Nucleobindin-2 (NUCB2) 12.0
 LDTP05868  Protein unc-119 homolog A (UNC119) 11.4
 LDTP00779  Trans-Golgi network integral membrane protein 2 (TGOLN2) 11.4
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 10.4
 LDTP19963  TraB domain-containing protein (TRABD) 10.3
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 10.3
 LDTP12412  Reticulon-4 (RTN4) 9.8
 LDTP13086  Syntaxin-18 (STX18) 9.5
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 9.4
 LDTP01359  Dynactin subunit 3 (DCTN3) 9.1
 LDTP13630  Neudesin (NENF) 8.9
 LDTP15623  Neuferricin (CYB5D2) 8.9
 LDTP06097  Reticulocalbin-2 (RCN2) 8.5
 LDTP14259  Testis-expressed protein 264 (TEX264) 8.5
 LDTP08594  Negative elongation factor C/D (NELFCD) 8.1
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 7.9
 LDTP18883  Protein CEBPZOS (CEBPZOS) 7.7
 LDTP12468  Diablo IAP-binding mitochondrial protein (DIABLO) 7.5
 LDTP11948  HEAT repeat-containing protein 1 (HEATR1) 7.5
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 7.2
 LDTP01075  Protein CutA (CUTA) 7.2
 LDTP00887  Calumenin (CALU) 7.2
 LDTP05688  Interleukin enhancer-binding factor 2 (ILF2) 7.2
 LDTP03775  RNA-binding protein FUS (FUS) 7.1
 LDTP09881  TATA-binding protein-associated factor 2N (TAF15) 7.1
 LDTP03614  Heterogeneous nuclear ribonucleoprotein H3 (HNRNPH3) 6.9
 LDTP00859  Synaptogyrin-2 (SYNGR2) 6.9
 LDTP10056  Protein FAM162A (FAM162A) 6.5
 LDTP07934  Synaptic vesicle glycoprotein 2A (SV2A) 6.5
 LDTP16080  Protein FAM114A2 (FAM114A2) 6.4
 LDTP12476  Translation initiation factor eIF2B subunit gamma (EIF2B3) 6.4
 LDTP06527  Alpha-internexin (INA) 6.3
 LDTP06022  Cold-inducible RNA-binding protein (CIRBP) 6.3
 LDTP02124  Keratin, type I cytoskeletal 18 (KRT18) 6.3
 LDTP10962  Heterogeneous nuclear ribonucleoprotein A/B (HNRNPAB) 6.2
 LDTP12395  Large ribosomal subunit protein mL40 (MRPL40) 6.2
 LDTP12755  OCIA domain-containing protein 1 (OCIAD1) 6.1
 LDTP13318  Translation initiation factor eIF2B subunit delta (EIF2B4) 6.1
 LDTP07368  Tetratricopeptide repeat protein 19, mitochondrial (TTC19) 6.0
 LDTP07274  MICOS complex subunit MIC13 (MICOS13) 5.9
 LDTP08248  NLR family member X1 (NLRX1) 5.9
 LDTP13104  Origin recognition complex subunit 3 (ORC3) 5.9
 LDTP03615  Heterogeneous nuclear ribonucleoprotein H (HNRNPH1) 5.8
 LDTP06068  MAGUK p55 subfamily member 2 (MPP2) 5.8
 LDTP04684  Nucleosome assembly protein 1-like 1 (NAP1L1) 5.8
 LDTP00991  Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP) 5.8
 LDTP11389  Bcl-2-like protein 13 (BCL2L13) 5.7
 LDTP00500  Heterogeneous nuclear ribonucleoprotein D-like (HNRNPDL) 5.7
 LDTP18132  Protein FAM136A (FAM136A) 5.7
 LDTP13922  Tudor and KH domain-containing protein (TDRKH) 5.7
 LDTP05035  Growth factor receptor-bound protein 2 (GRB2) 5.6
 LDTP04952  Heterogeneous nuclear ribonucleoprotein K (HNRNPK) 5.6

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587