Details of the Target

General Information of Target

Target ID LDTP13630
Target Name Neudesin (NENF)
Gene Name NENF
Gene ID 29937
Synonyms
CIR2; SPUF; Neudesin; Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47; Secreted protein of unknown function; SPUF protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEVLRRSSVFAAEIMDAFDRSPTDKELVAQAKALGREYVHARLLRAGLSWSAPERAAPVP
GRLAEVCAVLLRLGDELEMIRPSVYRNVARQLHISLQSEPVVTDAFLAVAGHIFSAGITW
GKVVSLYAVAAGLAVDCVRQAQPAMVHALVDCLGEFVRKTLATWLRRRGGWTDVLKCVVS
TDPGLRSHWLVAALCSFGRFLKAAFFVLLPER
Target Bioclass
Other
Family
Cytochrome b5 family, MAPR subfamily
Subcellular location
Secreted, extracellular space
Function
Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural progenitor cells at the embryonic stage and inhibits differentiation of astrocytes. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance.
Uniprot ID
Q9UMX5
Ensemble ID
ENST00000366988.5
HGNC ID
HGNC:30384

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Alkylaryl probe 3
 Probe Info  		5.96  LDD0381  [1]
FBPP2
 Probe Info  		362.11  LDD0318  [2]
C-Sul
 Probe Info  		8.50  LDD0066  [3]
TH211
 Probe Info  		Y137(18.79); Y88(12.03)  LDD0257  [4]
TH214
 Probe Info  		Y137(20.00)  LDD0258  [4]
TH216
 Probe Info  		Y137(20.00); Y88(6.83)  LDD0259  [4]
OPA-S-S-alkyne
 Probe Info  		K136(0.96)  LDD3494  [5]
Probe 1
 Probe Info  		Y55(28.65); Y88(180.36); Y142(16.69)  LDD3495  [6]
P11
 Probe Info  		15.60  LDD0201  [7]
HPAP
 Probe Info  		3.56  LDD0062  [8]
HHS-475
 Probe Info  		Y88(1.12); Y82(2.36)  LDD0264  [9]
AMP probe
 Probe Info  		N.A.  LDD0200  [10]
ATP probe
 Probe Info  		N.A.  LDD0199  [10]
SF
 Probe Info  		Y88(0.00); Y82(0.00)  LDD0028  [11]
1d-yne
 Probe Info  		N.A.  LDD0357  [12]
HHS-465
 Probe Info  		N.A.  LDD2240  [13]
HHS-482
 Probe Info  		Y88(0.65)  LDD2239  [14]
PAL-AfBPP Probe
Click To Hide/Show 96 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C001
 Probe Info  		5.21  LDD1711  [15]
C002
 Probe Info  		6.87  LDD1712  [15]
C010
 Probe Info  		7.52  LDD1719  [15]
C017
 Probe Info  		15.03  LDD1725  [15]
C022
 Probe Info  		10.20  LDD1728  [15]
C037
 Probe Info  		7.41  LDD1737  [15]
C038
 Probe Info  		14.62  LDD1738  [15]
C040
 Probe Info  		17.39  LDD1740  [15]
C041
 Probe Info  		16.00  LDD1741  [15]
C059
 Probe Info  		5.31  LDD1756  [15]
C063
 Probe Info  		10.63  LDD1760  [15]
C070
 Probe Info  		9.06  LDD1766  [15]
C082
 Probe Info  		8.88  LDD1774  [15]
C085
 Probe Info  		8.40  LDD1777  [15]
C087
 Probe Info  		12.38  LDD1779  [15]
C102
 Probe Info  		7.16  LDD1790  [15]
C107
 Probe Info  		9.25  LDD1794  [15]
C108
 Probe Info  		9.25  LDD1795  [15]
C110
 Probe Info  		5.03  LDD1797  [15]
C112
 Probe Info  		23.92  LDD1799  [15]
C131
 Probe Info  		9.92  LDD1813  [15]
C135
 Probe Info  		14.52  LDD1817  [15]
C138
 Probe Info  		5.58  LDD1820  [15]
C139
 Probe Info  		11.71  LDD1821  [15]
C141
 Probe Info  		20.25  LDD1823  [15]
C145
 Probe Info  		10.06  LDD1827  [15]
C173
 Probe Info  		7.16  LDD1853  [15]
C177
 Probe Info  		10.63  LDD1856  [15]
C179
 Probe Info  		5.43  LDD1858  [15]
C185
 Probe Info  		5.06  LDD1863  [15]
C194
 Probe Info  		13.00  LDD1870  [15]
C205
 Probe Info  		6.77  LDD1880  [15]
C206
 Probe Info  		19.29  LDD1881  [15]
C212
 Probe Info  		10.13  LDD1886  [15]
C214
 Probe Info  		6.15  LDD1888  [15]
C226
 Probe Info  		5.66  LDD1899  [15]
C227
 Probe Info  		7.73  LDD1900  [15]
C231
 Probe Info  		12.30  LDD1904  [15]
C233
 Probe Info  		11.00  LDD1906  [15]
C234
 Probe Info  		8.75  LDD1907  [15]
C243
 Probe Info  		15.89  LDD1916  [15]
C269
 Probe Info  		8.11  LDD1939  [15]
C272
 Probe Info  		5.78  LDD1942  [15]
C274
 Probe Info  		10.78  LDD1944  [15]
C277
 Probe Info  		9.85  LDD1947  [15]
C292
 Probe Info  		5.82  LDD1962  [15]
C299
 Probe Info  		5.70  LDD1968  [15]
C305
 Probe Info  		10.93  LDD1974  [15]
C313
 Probe Info  		16.91  LDD1980  [15]
C314
 Probe Info  		14.12  LDD1981  [15]
C320
 Probe Info  		6.15  LDD1986  [15]
C322
 Probe Info  		8.88  LDD1988  [15]
C326
 Probe Info  		11.71  LDD1990  [15]
C327
 Probe Info  		7.67  LDD1991  [15]
C338
 Probe Info  		11.39  LDD2001  [15]
C342
 Probe Info  		7.46  LDD2004  [15]
C346
 Probe Info  		16.22  LDD2007  [15]
C347
 Probe Info  		14.83  LDD2008  [15]
C348
 Probe Info  		11.71  LDD2009  [15]
C349
 Probe Info  		13.18  LDD2010  [15]
C350
 Probe Info  		25.11  LDD2011  [15]
C353
 Probe Info  		10.27  LDD2014  [15]
C354
 Probe Info  		57.28  LDD2015  [15]
C355
 Probe Info  		39.40  LDD2016  [15]
C356
 Probe Info  		15.89  LDD2017  [15]
C361
 Probe Info  		18.00  LDD2022  [15]
C373
 Probe Info  		13.45  LDD2033  [15]
C386
 Probe Info  		11.31  LDD2045  [15]
C390
 Probe Info  		76.11  LDD2049  [15]
C391
 Probe Info  		35.26  LDD2050  [15]
C392
 Probe Info  		7.62  LDD2051  [15]
C393
 Probe Info  		16.34  LDD2052  [15]
C394
 Probe Info  		6.77  LDD2053  [15]
C396
 Probe Info  		7.52  LDD2055  [15]
C420
 Probe Info  		14.52  LDD2075  [15]
C426
 Probe Info  		13.83  LDD2081  [15]
C428
 Probe Info  		6.50  LDD2083  [15]
C429
 Probe Info  		28.25  LDD2084  [15]
C430
 Probe Info  		9.00  LDD2085  [15]
C433
 Probe Info  		7.57  LDD2088  [15]
FFF probe11
 Probe Info  		20.00  LDD0471  [16]
FFF probe12
 Probe Info  		12.25  LDD0473  [16]
FFF probe13
 Probe Info  		20.00  LDD0475  [16]
FFF probe14
 Probe Info  		13.40  LDD0477  [16]
FFF probe15
 Probe Info  		14.07  LDD0478  [16]
FFF probe2
 Probe Info  		20.00  LDD0463  [16]
FFF probe3
 Probe Info  		20.00  LDD0464  [16]
FFF probe4
 Probe Info  		13.04  LDD0466  [16]
FFF probe6
 Probe Info  		14.34  LDD0467  [16]
FFF probe7
 Probe Info  		18.96  LDD0483  [16]
FFF probe9
 Probe Info  		15.70  LDD0470  [16]
JN0003
 Probe Info  		13.75  LDD0469  [16]
Kambe_26
 Probe Info  		2.05  LDD0134  [17]
Kambe_31
 Probe Info  		2.29  LDD0135  [17]
Kambe_55
 Probe Info  		3.23  LDD0129  [17]
OEA-DA
 Probe Info  		20.00  LDD0046  [18]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0088  C45 HEK-293T 15.60  LDD0201  [7]
 LDCM0189  Compound 16 HEK-293T 40.00  LDD0492  [16]
 LDCM0185  Compound 17 HEK-293T 11.81  LDD0504  [16]
 LDCM0182  Compound 18 HEK-293T 6.99  LDD0501  [16]
 LDCM0184  Compound 20 HEK-293T 6.83  LDD0503  [16]
 LDCM0191  Compound 21 HEK-293T 9.13  LDD0493  [16]
 LDCM0187  Compound 32 HEK-293T 27.69  LDD0507  [16]
 LDCM0190  Compound 34 HEK-293T 16.67  LDD0497  [16]
 LDCM0192  Compound 35 HEK-293T 11.05  LDD0491  [16]
 LDCM0193  Compound 36 HEK-293T 7.75  LDD0494  [16]
 LDCM0194  Compound 37 HEK-293T 12.20  LDD0498  [16]
 LDCM0195  Compound 38 HEK-293T 11.43  LDD0499  [16]
 LDCM0196  Compound 39 HEK-293T 11.11  LDD0496  [16]
 LDCM0197  Compound 40 HEK-293T 13.33  LDD0495  [16]
 LDCM0181  Compound 41 HEK-293T 5.24  LDD0502  [16]
 LDCM0183  Compound 42 HEK-293T 3.86  LDD0500  [16]
 LDCM0116  HHS-0101 DM93 Y88(1.12); Y82(2.36)  LDD0264  [9]
 LDCM0117  HHS-0201 DM93 Y88(1.43); Y82(3.42)  LDD0265  [9]
 LDCM0118  HHS-0301 DM93 Y88(0.61); Y82(0.97)  LDD0266  [9]
 LDCM0119  HHS-0401 DM93 Y88(0.73); Y82(1.66)  LDD0267  [9]
 LDCM0120  HHS-0701 DM93 Y88(1.02); Y82(8.30)  LDD0268  [9]
 LDCM0073  Kambe_cp3 PC-3 3.23  LDD0129  [17]
 LDCM0079  kambe_cp64 PC-3 2.29  LDD0135  [17]
 LDCM0078  kambe_cp70 PC-3 2.05  LDD0134  [17]
 LDCM0014  Panhematin HEK-293T 3.56  LDD0062  [8]

References

1 Hydrazines as versatile chemical biology probes and drug-discovery tools for cofactor-dependent enzymes. bioRxiv, 2020-06.
2 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
3 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
6 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
7 Discovery of Potent and Selective Inhibitors against Protein-Derived Electrophilic Cofactors. J Am Chem Soc. 2022 Mar 30;144(12):5377-5388. doi: 10.1021/jacs.1c12748. Epub 2022 Mar 2.
8 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
9 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
12 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
13 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
14 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
15 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
16 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
17 Mapping the protein interaction landscape for fully functionalized small-molecule probes in human cells. J Am Chem Soc. 2014 Jul 30;136(30):10777-82. doi: 10.1021/ja505517t. Epub 2014 Jul 21.
18 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570