Details of the Probe

General Information of Probe

Probe ID
LDPC0575
Probe Name
C354
Synonyms
Z3433406737
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 372.8
Lipid-water partition coefficient (xlogp) 2.9
Rotatable Bond Count (rotbonds) 8
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 6
Chemical Identifiers
Formula
C17H17ClN6O2
IUPAC Name
N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]-2-(3-chloropyridin-2-yl)-5-methoxypyrazole-3-carboxamide
Canonical SMILES
COC1=NN(C(=C1)C(=O)NCCC2(N=N2)CCC#C)C3=C(C=CC=N3)Cl
InChI
InChI=1S/C17H17ClN6O2/c1-3-4-7-17(22-23-17)8-10-20-16(25)13-11-14(26-2)21-24(13)15-12(18)6-5-9-19-15/h1,5-6,9,11H,4,7-8,10H2,2H3,(H,20,25)
InChIKey
HADFMTWAEWKDRT-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

78 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
5'-3' exonuclease PLD3 (PLD3) LDTP08476 8.57  LDD2015 
Acetyl-CoA acetyltransferase, cytosolic (ACAT2) LDTP11321 9.99  LDD2015 
Adenosine deaminase (ADA) LDTP01803 16.45  LDD2015 
Aldehyde dehydrogenase X, mitochondrial (ALDH1B1) LDTP03560 11.47  LDD2015 
Alpha-N-acetylglucosaminidase (NAGLU) LDTP04634 10.06  LDD2015 
Apoptosis-inducing factor 1, mitochondrial (AIFM1) LDTP01687 9.78  LDD2015 
ATP-dependent translocase ABCB1 (ABCB1) LDTP02254 9.38  LDD2015 
Beta-1,4-glucuronyltransferase 1 (B4GAT1) LDTP00783 12.91  LDD2015 
Beta-hexosaminidase subunit alpha (HEXA) LDTP02169 7.36  LDD2015 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 7.52  LDD2015 
Calcium/calmodulin-dependent protein kinase type 1 (CAMK1) LDTP06023 7.16  LDD2015 
Carbohydrate sulfotransferase 14 (CHST14) LDTP09096 8.17  LDD2015 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 20.39  LDD2015 
Cartilage-associated protein (CRTAP) LDTP01300 7.01  LDD2015 
Ceramide synthase 2 (CERS2) LDTP10329 13.55  LDD2015 
Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) LDTP03189 28.25  LDD2015 
Cytochrome b-c1 complex subunit 8 (UQCRQ) LDTP00486 8.40  LDD2015 
Cytochrome b-c1 complex subunit 9 (UQCR10) LDTP13189 9.38  LDD2015 
Cytochrome b5 type B (CYB5B) LDTP00697 16.22  LDD2015 
Cytochrome c oxidase subunit 2 (MT-CO2) LDTP01773 24.93  LDD2015 
DCN1-like protein 4 (DCUN1D4) LDTP09799 10.70  LDD2015 
Delta(24)-sterol reductase (DHCR24) LDTP06375 8.00  LDD2015 
Dihydrofolate reductase (DHFR) LDTP01769 13.93  LDD2015 
DNA replication licensing factor MCM6 (MCM6) LDTP06150 72.00  LDD2015 
E3 ubiquitin-protein ligase TRIM21 (TRIM21) LDTP03001 11.47  LDD2015 
Endoplasmic reticulum aminopeptidase 1 (ERAP1) LDTP12868 9.25  LDD2015 
Endoplasmic reticulum protein SC65 (P3H4) LDTP09874 7.06  LDD2015 
Endothelin-converting enzyme 1 (ECE1) LDTP04008 11.24  LDD2015 
Epoxide hydrolase 1 (EPHX1) LDTP02175 10.93  LDD2015 
FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) LDTP10188 11.71  LDD2015 
Ferrochelatase, mitochondrial (FECH) LDTP03198 8.57  LDD2015 
Heme oxygenase 1 (HMOX1) LDTP02359 9.45  LDD2015 
Heme oxygenase 2 (HMOX2) LDTP03540 56.89  LDD2015 
Histone deacetylase 1 (HDAC1) LDTP05918 7.06  LDD2015 
Immunity-related GTPase family Q protein (IRGQ) LDTP19838 8.00  LDD2015 
Isochorismatase domain-containing protein 1 (ISOC1) LDTP19851 7.16  LDD2015 
Lanosterol 14-alpha demethylase (CYP51A1) LDTP06648 16.56  LDD2015 
Leucyl-cystinyl aminopeptidase (LNPEP) LDTP13358 11.55  LDD2015 
Lipid droplet-associated hydrolase (LDAH) LDTP16007 17.51  LDD2015 
Lysophosphatidic acid phosphatase type 6 (ACP6) LDTP12363 16.34  LDD2015 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 35.75  LDD2015 
Mannose-6-phosphate isomerase (MPI) LDTP03697 7.21  LDD2015 
Metalloendopeptidase OMA1, mitochondrial (OMA1) LDTP10249 8.28  LDD2015 
Methylsterol monooxygenase 1 (MSMO1) LDTP06477 7.36  LDD2015 
N-acetylglucosamine-6-sulfatase (GNS) LDTP02799 7.01  LDD2015 
NADH dehydrogenase 1 alpha subcomplex subunit 13 (NDUFA13) LDTP12959 7.89  LDD2015 
NADH dehydrogenase 1 beta subcomplex subunit 10 (NDUFB10) LDTP01743 21.11  LDD2015 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 15.14  LDD2015 
NADH dehydrogenase iron-sulfur protein 2, mitochondrial (NDUFS2) LDTP01168 8.22  LDD2015 
NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) LDTP01982 9.78  LDD2015 
Palmitoyl-protein thioesterase 1 (PPT1) LDTP04388 8.28  LDD2015 
Peptidyl-prolyl cis-trans isomerase FKBP14 (FKBP14) LDTP12720 12.64  LDD2015 
Peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A) LDTP05029 9.25  LDD2015 
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) LDTP14218 7.73  LDD2015 
Peroxisomal trans-2-enoyl-CoA reductase (PECR) LDTP11437 6.96  LDD2015 
Phospholipase A2 group XV (PLA2G15) LDTP09088 22.63  LDD2015 
Platelet-activating factor acetylhydrolase IB subunit alpha1 (PAFAH1B3) LDTP06315 8.22  LDD2015 
Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4) LDTP08859 13.93  LDD2015 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 25.11  LDD2015 
Prolyl endopeptidase-like (PREPL) LDTP06849 8.06  LDD2015 
Prostaglandin reductase 2 (PTGR2) LDTP08979 10.20  LDD2015 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 8.63  LDD2015 
Proteasome subunit beta type-4 (PSMB4) LDTP03417 15.35  LDD2015 
Proteasome subunit beta type-6 (PSMB6) LDTP03418 11.55  LDD2015 
Proteasome subunit beta type-7 (PSMB7) LDTP10862 8.28  LDD2015 
Protein O-GlcNAcase (OGA) LDTP00988 8.88  LDD2015 
Pterin-4-alpha-carbinolamine dehydratase (PCBD1) LDTP04925 11.63  LDD2015 
Puromycin-sensitive aminopeptidase (NPEPPS) LDTP04712 12.38  LDD2015 
Putative phospholipase B-like 2 (PLBD2) LDTP09256 13.93  LDD2015 
RAC-beta serine/threonine-protein kinase (AKT2) LDTP03608 8.34  LDD2015 
Retinal dehydrogenase 2 (ALDH1A2) LDTP01419 12.04  LDD2015 
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) LDTP00907 8.28  LDD2015 
Retinoid-inducible serine carboxypeptidase (SCPEP1) LDTP16032 18.25  LDD2015 
S-methyl-5'-thioadenosine phosphorylase (MTAP) LDTP05758 25.11  LDD2015 
Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) LDTP15484 23.92  LDD2015 
Serum paraoxonase/arylesterase 2 (PON2) LDTP06333 8.46  LDD2015 
Small ribosomal subunit protein uS3 (RPS3) LDTP03223 14.83  LDD2015 
Squalene synthase (FDFT1) LDTP03842 37.27  LDD2015 
------------------------------------------------------------------------------------
⏷ Show the Full List of 78 Enzyme
40 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
ATP synthase subunit a (MT-ATP6) LDTP01804 58.08  LDD2015 
Cytoskeleton-associated protein 4 (CKAP4) LDTP05511 7.57  LDD2015 
Derlin-1 (DERL1) LDTP11245 11.79  LDD2015 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 44.63  LDD2015 
Flotillin-1 (FLOT1) LDTP01366 7.57  LDD2015 
High affinity cationic amino acid transporter 1 (SLC7A1) LDTP03559 8.75  LDD2015 
Lysosome membrane protein 2 (SCARB2) LDTP06034 14.83  LDD2015 
Metaxin-1 (MTX1) LDTP05900 11.08  LDD2015 
Metaxin-2 (MTX2) LDTP01217 12.04  LDD2015 
MFS-type transporter SLC18B1 (SLC18B1) LDTP07462 11.96  LDD2015 
Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) LDTP00865 18.51  LDD2015 
Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) LDTP13983 7.06  LDD2015 
Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) LDTP10913 18.64  LDD2015 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 10.41  LDD2015 
Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) LDTP14099 10.06  LDD2015 
Mitochondrial ornithine transporter 1 (SLC25A15) LDTP14200 7.21  LDD2015 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 17.51  LDD2015 
Monocarboxylate transporter 1 (SLC16A1) LDTP04592 10.27  LDD2015 
Protein GPR107 (GPR107) LDTP07236 18.25  LDD2015 
Protein GPR108 (GPR108) LDTP12383 8.46  LDD2015 
Protein tweety homolog 3 (TTYH3) LDTP11615 9.78  LDD2015 
Putative divalent cation/proton antiporter TMEM165 (TMEM165) LDTP12207 14.03  LDD2015 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 21.71  LDD2015 
Secretory carrier-associated membrane protein 4 (SCAMP4) LDTP09987 7.01  LDD2015 
Sigma non-opioid intracellular receptor 1 (SIGMAR1) LDTP10958 13.27  LDD2015 
Sodium-coupled neutral amino acid symporter 2 (SLC38A2) LDTP10731 9.25  LDD2015 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 10.13  LDD2015 
Solute carrier family 35 member F2 (SLC35F2) LDTP17641 13.83  LDD2015 
StAR-related lipid transfer protein 7, mitochondrial (STARD7) LDTP18459 7.36  LDD2015 
Surfeit locus protein 4 (SURF4) LDTP00592 7.94  LDD2015 
Tetraspanin-3 (TSPAN3) LDTP16813 8.63  LDD2015 
Translocator protein (TSPO) LDTP03546 11.79  LDD2015 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 15.67  LDD2015 
Transmembrane 9 superfamily member 3 (TM9SF3) LDTP18448 6.96  LDD2015 
Transmembrane protein 126A (TMEM126A) LDTP15948 13.09  LDD2015 
Transmembrane protein 199 (TMEM199) LDTP08881 15.78  LDD2015 
Transmembrane protein 87A (TMEM87A) LDTP15481 10.06  LDD2015 
V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) LDTP09968 8.34  LDD2015 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 52.71  LDD2015 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 22.78  LDD2015 
------------------------------------------------------------------------------------
⏷ Show the Full List of 40 Transporter and channel
1 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Y-box-binding protein 1 (YBX1) LDTP05065 7.73  LDD2015 
------------------------------------------------------------------------------------
3 Immunoglobulin Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Basigin (BSG) LDTP03772 8.40  LDD2015 
HLA class I histocompatibility antigen, A alpha chain (HLA-A) LDTP02040 7.94  LDD2015 
IgG receptor FcRn large subunit p51 (FCGRT) LDTP04720 12.64  LDD2015 
------------------------------------------------------------------------------------
70 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Activated RNA polymerase II transcriptional coactivator p15 (SUB1) LDTP04595 7.57  LDD2015 
Alpha-internexin (INA) LDTP06527 7.46  LDD2015 
Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) LDTP08364 10.20  LDD2015 
Apolipoprotein L2 (APOL2) LDTP11076 15.56  LDD2015 
Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) LDTP13755 18.51  LDD2015 
Calumenin (CALU) LDTP00887 8.51  LDD2015 
Centrosomal protein 43 (CEP43) LDTP01652 10.27  LDD2015 
Clustered mitochondria protein homolog (CLUH) LDTP14486 9.38  LDD2015 
Cold-inducible RNA-binding protein (CIRBP) LDTP06022 7.73  LDD2015 
Collectin-12 (COLEC12) LDTP07031 9.19  LDD2015 
Craniofacial development protein 1 (CFDP1) LDTP16193 12.21  LDD2015 
CYFIP-related Rac1 interactor B (CYRIB) LDTP12642 7.31  LDD2015 
Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) LDTP13902 8.88  LDD2015 
Diablo IAP-binding mitochondrial protein (DIABLO) LDTP12468 8.06  LDD2015 
Dynactin subunit 3 (DCTN3) LDTP01359 8.94  LDD2015 
Endoplasmic reticulum-Golgi intermediate compartment protein 2 (ERGIC2) LDTP10775 7.06  LDD2015 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 13.55  LDD2015 
ER lumen protein-retaining receptor 3 (KDELR3) LDTP00850 24.08  LDD2015 
Eukaryotic translation initiation factor 5 (EIF5) LDTP04652 8.82  LDD2015 
Golgi apparatus protein 1 (GLG1) LDTP09920 7.89  LDD2015 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 11.96  LDD2015 
HAUS augmin-like complex subunit 4 (HAUS4) LDTP11978 8.75  LDD2015 
Keratin, type I cytoskeletal 18 (KRT18) LDTP02124 10.27  LDD2015 
Kinetochore protein Spc24 (SPC24) LDTP09069 8.34  LDD2015 
Large proline-rich protein BAG6 (BAG6) LDTP04094 10.20  LDD2015 
Large ribosomal subunit protein mL40 (MRPL40) LDTP12395 9.71  LDD2015 
Large ribosomal subunit protein mL55 (MRPL55) LDTP15269 99.73  LDD2015 
Mdm2-binding protein (MTBP) LDTP15714 9.71  LDD2015 
Microtubule nucleation factor SSNA1 (SSNA1) LDTP00871 19.70  LDD2015 
Mitochondrial fission process protein 1 (MTFP1) LDTP16192 25.63  LDD2015 
Mitochondrial import inner membrane translocase subunit Tim8 A (TIMM8A) LDTP00913 7.26  LDD2015 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 29.86  LDD2015 
Mitochondrial import receptor subunit TOM5 homolog (TOMM5) LDTP15420 8.88  LDD2015 
Neudesin (NENF) LDTP13630 57.28  LDD2015 
Neuferricin (CYB5D2) LDTP15623 28.44  LDD2015 
Nuclear autoantigenic sperm protein (NASP) LDTP04241 7.16  LDD2015 
Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1) LDTP11764 8.57  LDD2015 
Nucleobindin-1 (NUCB1) LDTP05375 8.40  LDD2015 
Nucleobindin-2 (NUCB2) LDTP05160 23.10  LDD2015 
Nucleosome assembly protein 1-like 1 (NAP1L1) LDTP04684 9.00  LDD2015 
Nucleosome assembly protein 1-like 4 (NAP1L4) LDTP10965 9.32  LDD2015 
Poly(U)-binding-splicing factor PUF60 (PUF60) LDTP13311 7.57  LDD2015 
Polyadenylate-binding protein-interacting protein 2 (PAIP2) LDTP11044 7.67  LDD2015 
Prefoldin subunit 2 (PFDN2) LDTP13309 7.01  LDD2015 
Prefoldin subunit 6 (PFDN6) LDTP00573 9.06  LDD2015 
Proline-rich protein PRCC (PRCC) LDTP09850 7.41  LDD2015 
Proteasome activator complex subunit 2 (PSME2) LDTP13519 7.52  LDD2015 
Protein bicaudal D homolog 2 (BICD2) LDTP09410 9.25  LDD2015 
Protein CutA (CUTA) LDTP01075 7.21  LDD2015 
Protein FAM114A2 (FAM114A2) LDTP16080 9.13  LDD2015 
Protein FAM162A (FAM162A) LDTP10056 8.28  LDD2015 
Protein SET (SET) LDTP05277 10.20  LDD2015 
Protein transport protein Sec23A (SEC23A) LDTP06390 16.34  LDD2015 
Protein UXT (UXT) LDTP13125 10.70  LDD2015 
Reticulocalbin-2 (RCN2) LDTP06097 15.24  LDD2015 
Reticulon-2 (RTN2) LDTP01167 7.52  LDD2015 
Retinol-binding protein 1 (RBP1) LDTP02345 99.73  LDD2015 
RNA-binding protein 3 (RBM3) LDTP05226 9.38  LDD2015 
RNA-binding protein FUS (FUS) LDTP03775 8.11  LDD2015 
Small ribosomal subunit protein eS10 (RPS10) LDTP04111 8.57  LDD2015 
Stathmin (STMN1) LDTP02869 7.62  LDD2015 
Syntaxin-10 (STX10) LDTP00986 10.48  LDD2015 
Syntaxin-7 (STX7) LDTP00632 9.06  LDD2015 
Tetraspanin-6 (TSPAN6) LDTP14458 21.26  LDD2015 
Tetratricopeptide repeat protein 19, mitochondrial (TTC19) LDTP07368 7.94  LDD2015 
Tetratricopeptide repeat protein 38 (TTC38) LDTP19372 26.17  LDD2015 
Tropomyosin alpha-1 chain (TPM1) LDTP02351 10.20  LDD2015 
Tropomyosin alpha-3 chain (TPM3) LDTP02165 6.96  LDD2015 
Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) LDTP15067 14.32  LDD2015 
Vimentin (VIM) LDTP02297 8.06  LDD2015 
------------------------------------------------------------------------------------
⏷ Show the Full List of 70 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD2015 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587