Details of the Target

General Information of Target

Target ID LDTP02351
Target Name Tropomyosin alpha-1 chain (TPM1)
Gene Name TPM1
Gene ID 7168
Synonyms
C15orf13; TMSA; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKY
SEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAA
DESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAE
ERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAE
FAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI
Target Bioclass
Other
Family
Tropomyosin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Uniprot ID
P09493
Ensemble ID
ENST00000267996.11
HGNC ID
HGNC:12010
ChEMBL ID
CHEMBL4295705

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HDQP1 SNV: p.E117Ter .
NCIH1155 SNV: p.A22V; p.C190S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K231(1.41); K248(10.00)  LDD0277  [1]
Probe 1
 Probe Info  		Y221(74.35); Y261(19.91)  LDD3495  [2]
DBIA
 Probe Info  		C190(1.86)  LDD3337  [3]
HHS-475
 Probe Info  		Y162(0.89)  LDD0264  [4]
HHS-465
 Probe Info  		Y162(10.00)  LDD2237  [5]
m-APA
 Probe Info  		N.A.  LDD2234  [6]
AZ-9
 Probe Info  		D100(0.00); E97(0.00)  LDD0395  [7]
Acrolein
 Probe Info  		N.A.  LDD0217  [8]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [8]
Methacrolein
 Probe Info  		N.A.  LDD0218  [8]
TER-AC
 Probe Info  		N.A.  LDD0426  [9]
PAL-AfBPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C003
 Probe Info  		24.59  LDD1713  [10]
C052
 Probe Info  		11.96  LDD1750  [10]
C053
 Probe Info  		5.35  LDD1751  [10]
C210
 Probe Info  		46.85  LDD1884  [10]
C212
 Probe Info  		5.58  LDD1886  [10]
C284
 Probe Info  		31.34  LDD1954  [10]
C305
 Probe Info  		9.65  LDD1974  [10]
C313
 Probe Info  		18.38  LDD1980  [10]
C314
 Probe Info  		12.21  LDD1981  [10]
C317
 Probe Info  		6.77  LDD1983  [10]
C346
 Probe Info  		12.38  LDD2007  [10]
C347
 Probe Info  		5.50  LDD2008  [10]
C354
 Probe Info  		10.20  LDD2015  [10]
C362
 Probe Info  		27.10  LDD2023  [10]
C388
 Probe Info  		39.40  LDD2047  [10]
C391
 Probe Info  		13.83  LDD2050  [10]
C397
 Probe Info  		9.51  LDD2056  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [8]
 LDCM0116  HHS-0101 DM93 Y162(0.89)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y162(0.77)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y162(0.89)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y162(0.86)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y162(0.74)  LDD0268  [4]
 LDCM0107  IAA HeLa N.A.  LDD0221  [8]
 LDCM0022  KB02 786-O C190(1.17)  LDD2247  [3]
 LDCM0023  KB03 786-O C190(1.20)  LDD2664  [3]
 LDCM0024  KB05 MSTO-211H C190(1.86)  LDD3337  [3]
 LDCM0109  NEM HeLa N.A.  LDD0223  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
26S proteasome regulatory subunit 8 (PSMC5) AAA ATPase family P62195
Other
Click To Hide/Show 13 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Biogenesis of lysosome-related organelles complex 1 subunit 6 (BLOC1S6) BLOC1S6 family Q9UL45
Protein FAM9C (FAM9C) FAM9 family Q8IZT9
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
Interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) IFIT family O14879
KxDL motif-containing protein 1 (KXD1) KXD1 family Q9BQD3
Mitotic spindle assembly checkpoint protein MAD1 (MAD1L1) MAD1 family Q9Y6D9
Synaptonemal complex central element protein 1 (SYCE1) SYCE family Q8N0S2
Troponin T, slow skeletal muscle (TNNT1) Troponin T family P13805
UPF0500 protein C1orf216 (C1orf216) UPF0500 family Q8TAB5
Cancer-associated gene 1 protein (CAGE1) . Q8TC20
Coiled-coil domain-containing protein 96 (CCDC96) . Q2M329
TCF3 fusion partner (TFPT) . P0C1Z6
YEATS domain-containing protein 4 (YEATS4) . O95619

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Artenimol . DB11638
Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Phenethyl Isothiocyanate Small molecular drug DB12695

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587