Details of the Probe

General Information of Probe

Probe ID
LDPC0543
Probe Name
C317
Synonyms
Z4225935356
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 340.4
Lipid-water partition coefficient (xlogp) 1.4
Rotatable Bond Count (rotbonds) 8
Hydrogen Bond Donor Count (hbonddonor) 2
Hydrogen Bond Acceptor Count (hbondacc) 7
Chemical Identifiers
Formula
C13H16N4O3S2
IUPAC Name
N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]-4-(methylsulfamoyl)thiophene-2-carboxamide
Canonical SMILES
CNS(=O)(=O)C1=CSC(=C1)C(=O)NCCC2(N=N2)CCC#C
InChI
InChI=1S/C13H16N4O3S2/c1-3-4-5-13(16-17-13)6-7-15-12(18)11-8-10(9-21-11)22(19,20)14-2/h1,8-9,14H,4-7H2,2H3,(H,15,18)
InChIKey
VZHFSPLSINUJHI-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

55 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
3-ketodihydrosphingosine reductase (KDSR) LDTP05476 6.23  LDD1983 
7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) LDTP10032 10.85  LDD1983 
Acetyl-CoA acetyltransferase, cytosolic (ACAT2) LDTP11321 14.42  LDD1983 
Adenosine deaminase (ADA) LDTP01803 8.17  LDD1983 
ADP-dependent glucokinase (ADPGK) LDTP11127 6.73  LDD1983 
ADP-ribosylation factor-like protein 10 (ARL10) LDTP19745 7.06  LDD1983 
Atypical kinase COQ8A, mitochondrial (COQ8A) LDTP09285 5.74  LDD1983 
Carbohydrate sulfotransferase 14 (CHST14) LDTP09096 7.41  LDD1983 
Ceramide glucosyltransferase (UGCG) LDTP06617 10.78  LDD1983 
Ceramide synthase 2 (CERS2) LDTP10329 7.57  LDD1983 
Cytochrome b-c1 complex subunit 9 (UQCR10) LDTP13189 9.78  LDD1983 
Cytochrome b5 type B (CYB5B) LDTP00697 6.96  LDD1983 
Cytochrome P450 20A1 (CYP20A1) LDTP17410 6.06  LDD1983 
DCN1-like protein 4 (DCUN1D4) LDTP09799 18.25  LDD1983 
dCTP pyrophosphatase 1 (DCTPP1) LDTP12013 10.85  LDD1983 
Delta(24)-sterol reductase (DHCR24) LDTP06375 9.45  LDD1983 
Deoxyhypusine hydroxylase (DOHH) LDTP11225 6.15  LDD1983 
Endoplasmic reticulum aminopeptidase 1 (ERAP1) LDTP12868 9.71  LDD1983 
Endoplasmic reticulum aminopeptidase 2 (ERAP2) LDTP07503 6.28  LDD1983 
Endoplasmic reticulum protein SC65 (P3H4) LDTP09874 6.32  LDD1983 
Endoplasmic reticulum transmembrane helix translocase (ATP13A1) LDTP12279 6.77  LDD1983 
Glutathione S-transferase omega-1 (GSTO1) LDTP05128 9.65  LDD1983 
Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM) LDTP12253 7.46  LDD1983 
Heme oxygenase 1 (HMOX1) LDTP02359 7.57  LDD1983 
Heme oxygenase 2 (HMOX2) LDTP03540 13.55  LDD1983 
Heparan sulfate 2-O-sulfotransferase 1 (HS2ST1) LDTP07990 6.02  LDD1983 
Histone-lysine N-methyltransferase 2A (KMT2A) LDTP05396 12.82  LDD1983 
Lanosterol 14-alpha demethylase (CYP51A1) LDTP06648 10.20  LDD1983 
Lipid droplet-associated hydrolase (LDAH) LDTP16007 29.86  LDD1983 
Lysophosphatidic acid phosphatase type 6 (ACP6) LDTP12363 8.11  LDD1983 
Lysosomal Pro-X carboxypeptidase (PRCP) LDTP04004 5.74  LDD1983 
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) LDTP03677 9.13  LDD1983 
N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) LDTP11273 5.94  LDD1983 
NAD(P) transhydrogenase, mitochondrial (NNT) LDTP05862 13.93  LDD1983 
NAD-dependent protein deacetylase sirtuin-2 (SIRT2) LDTP08598 6.28  LDD1983 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 8.57  LDD1983 
NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) LDTP01982 13.09  LDD1983 
Peptidyl-prolyl cis-trans isomerase FKBP14 (FKBP14) LDTP12720 8.82  LDD1983 
Peptidyl-prolyl cis-trans isomerase FKBP9 (FKBP9) LDTP01562 6.23  LDD1983 
Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) LDTP06307 6.23  LDD1983 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 8.17  LDD1983 
Prostaglandin reductase 3 (PTGR3) LDTP15422 10.48  LDD1983 
Proteasome subunit beta type-4 (PSMB4) LDTP03417 10.20  LDD1983 
Protein arginine N-methyltransferase 9 (PRMT9) LDTP07525 5.74  LDD1983 
Protein O-GlcNAcase (OGA) LDTP00988 13.27  LDD1983 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 11.47  LDD1983 
Puromycin-sensitive aminopeptidase (NPEPPS) LDTP04712 9.58  LDD1983 
RAC-beta serine/threonine-protein kinase (AKT2) LDTP03608 14.42  LDD1983 
Ribitol 5-phosphate transferase FKRP (FKRP) LDTP12109 6.06  LDD1983 
S-methyl-5'-thioadenosine phosphorylase (MTAP) LDTP05758 7.31  LDD1983 
Thimet oligopeptidase (THOP1) LDTP04542 6.82  LDD1983 
Ubiquitin carboxyl-terminal hydrolase 47 (USP47) LDTP10495 5.78  LDD1983 
UDP-glucuronic acid decarboxylase 1 (UXS1) LDTP09074 8.22  LDD1983 
V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) LDTP14030 7.31  LDD1983 
Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) LDTP08728 7.21  LDD1983 
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⏷ Show the Full List of 55 Enzyme
26 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
ATP synthase subunit a (MT-ATP6) LDTP01804 10.27  LDD1983 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 9.92  LDD1983 
BRI3-binding protein (BRI3BP) LDTP15648 8.88  LDD1983 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 8.17  LDD1983 
Cytoskeleton-associated protein 4 (CKAP4) LDTP05511 10.70  LDD1983 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 10.34  LDD1983 
Metaxin-1 (MTX1) LDTP05900 14.03  LDD1983 
Metaxin-3 (MTX3) LDTP14970 7.06  LDD1983 
Mitochondrial dicarboxylate carrier (SLC25A10) LDTP13174 5.86  LDD1983 
Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) LDTP10913 12.21  LDD1983 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 20.25  LDD1983 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 28.64  LDD1983 
Mpv17-like protein 2 (MPV17L2) LDTP14952 6.36  LDD1983 
Peroxisomal membrane protein 2 (PXMP2) LDTP16069 9.71  LDD1983 
Protein GPR107 (GPR107) LDTP07236 9.19  LDD1983 
Protein wntless homolog (WLS) LDTP07156 6.59  LDD1983 
Sideroflexin-4 (SFXN4) LDTP07531 5.82  LDD1983 
Sigma non-opioid intracellular receptor 1 (SIGMAR1) LDTP10958 6.50  LDD1983 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 8.06  LDD1983 
Surfeit locus protein 4 (SURF4) LDTP00592 5.74  LDD1983 
Translocating chain-associated membrane protein 1 (TRAM1) LDTP06426 6.77  LDD1983 
Translocator protein (TSPO) LDTP03546 9.92  LDD1983 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 10.41  LDD1983 
Transmembrane protein 160 (TMEM160) LDTP20002 6.06  LDD1983 
Transmembrane protein 199 (TMEM199) LDTP08881 6.92  LDD1983 
V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) LDTP09968 9.65  LDD1983 
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⏷ Show the Full List of 26 Transporter and channel
50 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) LDTP08364 8.00  LDD1983 
Apolipoprotein L2 (APOL2) LDTP11076 11.24  LDD1983 
BLOC-2 complex member HPS3 (HPS3) LDTP09994 9.51  LDD1983 
Calumenin (CALU) LDTP00887 5.98  LDD1983 
Centrosomal protein 43 (CEP43) LDTP01652 6.92  LDD1983 
Clustered mitochondria protein homolog (CLUH) LDTP14486 15.78  LDD1983 
Coiled-coil domain-containing protein 6 (CCDC6) LDTP06515 6.59  LDD1983 
CYFIP-related Rac1 interactor B (CYRIB) LDTP12642 10.20  LDD1983 
Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) LDTP13902 6.50  LDD1983 
Diablo IAP-binding mitochondrial protein (DIABLO) LDTP12468 7.11  LDD1983 
Dymeclin (DYM) LDTP08000 29.45  LDD1983 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 13.18  LDD1983 
ER lumen protein-retaining receptor 2 (KDELR2) LDTP03678 19.70  LDD1983 
ER lumen protein-retaining receptor 3 (KDELR3) LDTP00850 7.16  LDD1983 
Eukaryotic translation initiation factor 5 (EIF5) LDTP04652 7.67  LDD1983 
Exosome complex component RRP4 (EXOSC2) LDTP05993 6.87  LDD1983 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 10.20  LDD1983 
GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1) LDTP06255 6.11  LDD1983 
HAUS augmin-like complex subunit 4 (HAUS4) LDTP11978 8.63  LDD1983 
Hepatoma-derived growth factor (HDGF) LDTP04483 5.90  LDD1983 
Immunoglobulin-binding protein 1 (IGBP1) LDTP05089 6.06  LDD1983 
Inhibitor of nuclear factor kappa-B kinase-interacting protein (IKBIP) LDTP07891 10.06  LDD1983 
Keratin, type I cytoskeletal 18 (KRT18) LDTP02124 7.57  LDD1983 
Kinetochore protein Spc24 (SPC24) LDTP09069 9.65  LDD1983 
Large proline-rich protein BAG6 (BAG6) LDTP04094 13.83  LDD1983 
Large ribosomal subunit protein mL55 (MRPL55) LDTP15269 39.40  LDD1983 
Microtubule nucleation factor SSNA1 (SSNA1) LDTP00871 6.87  LDD1983 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 13.09  LDD1983 
Mitochondrial import receptor subunit TOM5 homolog (TOMM5) LDTP15420 10.13  LDD1983 
Neuferricin (CYB5D2) LDTP15623 11.39  LDD1983 
Neurofilament light polypeptide (NEFL) LDTP02180 6.36  LDD1983 
Nucleobindin-2 (NUCB2) LDTP05160 11.39  LDD1983 
Nucleosome assembly protein 1-like 4 (NAP1L4) LDTP10965 6.50  LDD1983 
Periphilin-1 (PPHLN1) LDTP09183 5.82  LDD1983 
Poly(U)-binding-splicing factor PUF60 (PUF60) LDTP13311 8.17  LDD1983 
Polyadenylate-binding protein-interacting protein 2 (PAIP2) LDTP11044 6.63  LDD1983 
Proline-, glutamic acid- and leucine-rich protein 1 (PELP1) LDTP08707 6.68  LDD1983 
Protein CutA (CUTA) LDTP01075 8.11  LDD1983 
Protein ecdysoneless homolog (ECD) LDTP01711 10.56  LDD1983 
Protein FAM114A2 (FAM114A2) LDTP16080 9.65  LDD1983 
Protein SET (SET) LDTP05277 9.32  LDD1983 
Reticulocalbin-2 (RCN2) LDTP06097 8.94  LDD1983 
Small ribosomal subunit protein eS10 (RPS10) LDTP04111 6.06  LDD1983 
Syntaxin-18 (STX18) LDTP13086 6.63  LDD1983 
T-complex protein 1 subunit gamma (CCT3) LDTP04249 5.86  LDD1983 
Tetratricopeptide repeat protein 21B (TTC21B) LDTP08073 6.50  LDD1983 
Tropomyosin alpha-1 chain (TPM1) LDTP02351 6.77  LDD1983 
Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) LDTP15067 10.48  LDD1983 
Vacuolar protein sorting-associated protein 51 homolog (VPS51) LDTP13338 6.28  LDD1983 
Vimentin (VIM) LDTP02297 7.11  LDD1983 
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⏷ Show the Full List of 50 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD1983 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587