Details of the Target

General Information of Target

Target ID LDTP01039
Target Name Protein-S-isoprenylcysteine O-methyltransferase (ICMT)
Gene Name ICMT
Gene ID 23463
Synonyms
PCCMT; Protein-S-isoprenylcysteine O-methyltransferase; EC 2.1.1.100; Isoprenylcysteine carboxylmethyltransferase; Prenylated protein carboxyl methyltransferase; PPMT; Prenylcysteine carboxyl methyltransferase; pcCMT
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGCAARAPPGSEARLSLATFLLGASVLALPLLTRAGLQGRTGLALYVAGLNALLLLLYR
PPRYQIAIRACFLGFVFGCGTLLSFSQSSWSHFGWYMCSLSLFHYSEYLVTAVNNPKSLS
LDSFLLNHSLEYTVAALSSWLEFTLENIFWPELKQITWLSVTGLLMVVFGECLRKAAMFT
AGSNFNHVVQNEKSDTHTLVTSGVYAWFRHPSYVGWFYWSIGTQVMLCNPICGVSYALTV
WRFFRDRTEEEEISLIHFFGEEYLEYKKRVPTGLPFIKGVKVDL
Target Bioclass
Enzyme
Family
Class VI-like SAM-binding methyltransferase superfamily, Isoprenylcysteine carboxyl methyltransferase family
Subcellular location
Endoplasmic reticulum membrane
Function Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
Uniprot ID
O60725
Ensemble ID
ENST00000343813.10
HGNC ID
HGNC:5350
ChEMBL ID
CHEMBL4699

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DA-P2
 Probe Info  		5.57  LDD0348  [1]
CHEMBL5175495
 Probe Info  		9.10  LDD0196  [2]
STPyne
 Probe Info  		K278(7.57); K281(6.11)  LDD0277  [3]
Johansson_61
 Probe Info  		_(11.53)  LDD1489  [4]
EA-probe
 Probe Info  		N.A.  LDD0440  [5]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [6]
TFBX
 Probe Info  		N.A.  LDD0027  [6]
IPM
 Probe Info  		N.A.  LDD0005  [7]
AOyne
 Probe Info  		12.20  LDD0443  [8]
PAL-AfBPP Probe
Click To Hide/Show 171 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C001
 Probe Info  		5.78  LDD1711  [9]
C003
 Probe Info  		11.96  LDD1713  [9]
C004
 Probe Info  		10.56  LDD1714  [9]
C011
 Probe Info  		6.19  LDD1720  [9]
C017
 Probe Info  		9.25  LDD1725  [9]
C022
 Probe Info  		16.68  LDD1728  [9]
C027
 Probe Info  		7.73  LDD1733  [9]
C040
 Probe Info  		19.16  LDD1740  [9]
C041
 Probe Info  		19.29  LDD1741  [9]
C049
 Probe Info  		6.82  LDD1747  [9]
C053
 Probe Info  		6.54  LDD1751  [9]
C055
 Probe Info  		24.93  LDD1752  [9]
C056
 Probe Info  		35.02  LDD1753  [9]
C063
 Probe Info  		28.64  LDD1760  [9]
C064
 Probe Info  		13.00  LDD1761  [9]
C070
 Probe Info  		16.34  LDD1766  [9]
C072
 Probe Info  		11.08  LDD1768  [9]
C082
 Probe Info  		10.06  LDD1774  [9]
C085
 Probe Info  		6.92  LDD1777  [9]
C087
 Probe Info  		15.67  LDD1779  [9]
C091
 Probe Info  		48.50  LDD1782  [9]
C092
 Probe Info  		42.52  LDD1783  [9]
C094
 Probe Info  		79.89  LDD1785  [9]
C100
 Probe Info  		8.40  LDD1789  [9]
C106
 Probe Info  		50.56  LDD1793  [9]
C107
 Probe Info  		10.78  LDD1794  [9]
C108
 Probe Info  		15.24  LDD1795  [9]
C112
 Probe Info  		64.00  LDD1799  [9]
C129
 Probe Info  		8.11  LDD1811  [9]
C130
 Probe Info  		10.93  LDD1812  [9]
C134
 Probe Info  		42.22  LDD1816  [9]
C135
 Probe Info  		11.31  LDD1817  [9]
C139
 Probe Info  		17.51  LDD1821  [9]
C140
 Probe Info  		9.65  LDD1822  [9]
C141
 Probe Info  		18.38  LDD1823  [9]
C143
 Probe Info  		19.70  LDD1825  [9]
C145
 Probe Info  		15.45  LDD1827  [9]
C147
 Probe Info  		7.73  LDD1829  [9]
C153
 Probe Info  		29.24  LDD1834  [9]
C158
 Probe Info  		17.27  LDD1838  [9]
C159
 Probe Info  		19.43  LDD1839  [9]
C160
 Probe Info  		12.55  LDD1840  [9]
C161
 Probe Info  		30.70  LDD1841  [9]
C163
 Probe Info  		7.36  LDD1843  [9]
C165
 Probe Info  		14.93  LDD1845  [9]
C166
 Probe Info  		20.53  LDD1846  [9]
C169
 Probe Info  		36.76  LDD1849  [9]
C174
 Probe Info  		7.46  LDD1854  [9]
C177
 Probe Info  		7.31  LDD1856  [9]
C178
 Probe Info  		27.28  LDD1857  [9]
C183
 Probe Info  		15.14  LDD1861  [9]
C185
 Probe Info  		27.28  LDD1863  [9]
C186
 Probe Info  		23.43  LDD1864  [9]
C187
 Probe Info  		31.12  LDD1865  [9]
C191
 Probe Info  		18.77  LDD1868  [9]
C193
 Probe Info  		10.34  LDD1869  [9]
C194
 Probe Info  		18.25  LDD1870  [9]
C196
 Probe Info  		17.75  LDD1872  [9]
C197
 Probe Info  		8.17  LDD1873  [9]
C198
 Probe Info  		16.34  LDD1874  [9]
C201
 Probe Info  		99.73  LDD1877  [9]
C204
 Probe Info  		7.89  LDD1879  [9]
C205
 Probe Info  		5.35  LDD1880  [9]
C206
 Probe Info  		58.08  LDD1881  [9]
C210
 Probe Info  		62.68  LDD1884  [9]
C211
 Probe Info  		15.45  LDD1885  [9]
C213
 Probe Info  		26.91  LDD1887  [9]
C215
 Probe Info  		8.17  LDD1889  [9]
C216
 Probe Info  		7.73  LDD1890  [9]
C218
 Probe Info  		38.32  LDD1892  [9]
C219
 Probe Info  		7.01  LDD1893  [9]
C220
 Probe Info  		25.11  LDD1894  [9]
C222
 Probe Info  		9.06  LDD1896  [9]
C225
 Probe Info  		10.13  LDD1898  [9]
C226
 Probe Info  		6.45  LDD1899  [9]
C228
 Probe Info  		50.91  LDD1901  [9]
C229
 Probe Info  		24.42  LDD1902  [9]
C231
 Probe Info  		58.49  LDD1904  [9]
C232
 Probe Info  		99.73  LDD1905  [9]
C233
 Probe Info  		24.08  LDD1906  [9]
C234
 Probe Info  		14.72  LDD1907  [9]
C235
 Probe Info  		47.18  LDD1908  [9]
C238
 Probe Info  		24.59  LDD1911  [9]
C239
 Probe Info  		9.19  LDD1912  [9]
C240
 Probe Info  		8.82  LDD1913  [9]
C243
 Probe Info  		19.03  LDD1916  [9]
C244
 Probe Info  		49.18  LDD1917  [9]
C246
 Probe Info  		26.91  LDD1919  [9]
C249
 Probe Info  		16.56  LDD1922  [9]
C252
 Probe Info  		10.56  LDD1925  [9]
C258
 Probe Info  		5.35  LDD1931  [9]
C264
 Probe Info  		28.05  LDD1935  [9]
C265
 Probe Info  		35.51  LDD1936  [9]
C266
 Probe Info  		9.99  LDD1937  [9]
C270
 Probe Info  		10.27  LDD1940  [9]
C271
 Probe Info  		7.36  LDD1941  [9]
C273
 Probe Info  		7.73  LDD1943  [9]
C277
 Probe Info  		16.11  LDD1947  [9]
C278
 Probe Info  		86.22  LDD1948  [9]
C280
 Probe Info  		19.29  LDD1950  [9]
C282
 Probe Info  		40.50  LDD1952  [9]
C284
 Probe Info  		35.51  LDD1954  [9]
C285
 Probe Info  		36.76  LDD1955  [9]
C287
 Probe Info  		12.64  LDD1957  [9]
C288
 Probe Info  		11.96  LDD1958  [9]
C289
 Probe Info  		76.11  LDD1959  [9]
C290
 Probe Info  		10.13  LDD1960  [9]
C293
 Probe Info  		79.89  LDD1963  [9]
C296
 Probe Info  		28.44  LDD1966  [9]
C297
 Probe Info  		21.41  LDD1967  [9]
C299
 Probe Info  		13.83  LDD1968  [9]
C303
 Probe Info  		5.06  LDD1972  [9]
C305
 Probe Info  		10.34  LDD1974  [9]
C307
 Probe Info  		5.39  LDD1975  [9]
C310
 Probe Info  		10.48  LDD1977  [9]
C311
 Probe Info  		5.17  LDD1978  [9]
C314
 Probe Info  		17.03  LDD1981  [9]
C317
 Probe Info  		11.47  LDD1983  [9]
C322
 Probe Info  		7.57  LDD1988  [9]
C326
 Probe Info  		19.84  LDD1990  [9]
C327
 Probe Info  		7.57  LDD1991  [9]
C333
 Probe Info  		15.67  LDD1996  [9]
C335
 Probe Info  		7.73  LDD1998  [9]
C338
 Probe Info  		22.01  LDD2001  [9]
C339
 Probe Info  		15.35  LDD2002  [9]
C342
 Probe Info  		5.98  LDD2004  [9]
C343
 Probe Info  		23.10  LDD2005  [9]
C346
 Probe Info  		16.68  LDD2007  [9]
C347
 Probe Info  		6.23  LDD2008  [9]
C348
 Probe Info  		22.78  LDD2009  [9]
C349
 Probe Info  		54.19  LDD2010  [9]
C350
 Probe Info  		74.03  LDD2011  [9]
C351
 Probe Info  		12.82  LDD2012  [9]
C353
 Probe Info  		16.91  LDD2014  [9]
C355
 Probe Info  		74.03  LDD2016  [9]
C356
 Probe Info  		17.51  LDD2017  [9]
C357
 Probe Info  		6.15  LDD2018  [9]
C360
 Probe Info  		13.18  LDD2021  [9]
C361
 Probe Info  		18.51  LDD2022  [9]
C362
 Probe Info  		95.67  LDD2023  [9]
C363
 Probe Info  		39.12  LDD2024  [9]
C364
 Probe Info  		41.64  LDD2025  [9]
C366
 Probe Info  		19.16  LDD2027  [9]
C367
 Probe Info  		9.38  LDD2028  [9]
C373
 Probe Info  		8.17  LDD2033  [9]
C376
 Probe Info  		17.39  LDD2036  [9]
C378
 Probe Info  		8.06  LDD2037  [9]
C380
 Probe Info  		20.82  LDD2039  [9]
C382
 Probe Info  		26.54  LDD2041  [9]
C383
 Probe Info  		22.47  LDD2042  [9]
C386
 Probe Info  		9.92  LDD2045  [9]
C388
 Probe Info  		65.34  LDD2047  [9]
C390
 Probe Info  		53.08  LDD2049  [9]
C391
 Probe Info  		15.56  LDD2050  [9]
C399
 Probe Info  		9.32  LDD2058  [9]
C403
 Probe Info  		52.71  LDD2061  [9]
C405
 Probe Info  		5.10  LDD2063  [9]
C407
 Probe Info  		29.24  LDD2064  [9]
C409
 Probe Info  		7.11  LDD2066  [9]
C413
 Probe Info  		15.45  LDD2069  [9]
C419
 Probe Info  		19.29  LDD2074  [9]
C420
 Probe Info  		11.08  LDD2075  [9]
C424
 Probe Info  		41.64  LDD2079  [9]
C426
 Probe Info  		52.35  LDD2081  [9]
C429
 Probe Info  		22.94  LDD2084  [9]
C431
 Probe Info  		28.84  LDD2086  [9]
FFF probe11
 Probe Info  		20.00  LDD0471  [10]
FFF probe13
 Probe Info  		20.00  LDD0475  [10]
FFF probe14
 Probe Info  		15.09  LDD0477  [10]
FFF probe2
 Probe Info  		20.00  LDD0463  [10]
OEA-DA
 Probe Info  		19.95  LDD0046  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [5]
 LDCM0616  Fragment61 Jurkat _(11.53)  LDD1489  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase RNF19B (RNF19B) RBR family Q6ZMZ0
ADP-ribosylation factor-like protein 13B (ARL13B) Arf family Q3SXY8
E3 ubiquitin-protein ligase TRIM32 (TRIM32) TRIM/RBCC family Q13049
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
G protein-activated inward rectifier potassium channel 2 (KCNJ6) Inward rectifier-type potassium channel family P48051
Aquaporin-6 (AQP6) MIP/aquaporin (TC 1.A.8) family Q13520
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein jagunal homolog 1 (JAGN1) Jagunal family Q8N5M9
PDZK1-interacting protein 1 (PDZK1IP1) . Q13113

References

1 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
2 Charting the Chemical Space of Acrylamide-Based Inhibitors of zDHHC20. ACS Med Chem Lett. 2022 Sep 26;13(10):1648-1654. doi: 10.1021/acsmedchemlett.2c00336. eCollection 2022 Oct 13.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
5 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
11 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570