Details of the Probe

General Information of Probe

Probe ID
LDPC0293
Probe Name
C027
Synonyms
Z4225960480
Probe Type
PAL-AfBPP Probe
Structure
2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 294.31
Lipid-water partition coefficient (xlogp) 1
Rotatable Bond Count (rotbonds) 6
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 6
Chemical Identifiers
Formula
C15H14N6O
IUPAC Name
N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]pyrido[2,3-b]pyrazine-8-carboxamide
Canonical SMILES
C#CCCC1(N=N1)CCNC(=O)C2=C3C(=NC=C2)N=CC=N3
InChI
InChI=1S/C15H14N6O/c1-2-3-5-15(20-21-15)6-8-19-14(22)11-4-7-17-13-12(11)16-9-10-18-13/h1,4,7,9-10H,3,5-6,8H2,(H,19,22)
InChIKey
ALWVNDCCVDFJKL-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

84 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
26S proteasome non-ATPase regulatory subunit 8 (PSMD8) LDTP04196 8.82  LDD1733 
5'-3' exonuclease PLD3 (PLD3) LDTP08476 7.62  LDD1733 
Acyl-CoA 6-desaturase (FADS2) LDTP01699 5.90  LDD1733 
Adenosine kinase (ADK) LDTP04689 7.67  LDD1733 
Adipocyte plasma membrane-associated protein (APMAP) LDTP12293 11.88  LDD1733 
ADP-dependent glucokinase (ADPGK) LDTP11127 11.79  LDD1733 
ADP-ribosylation factor-like protein 15 (ARL15) LDTP18489 6.54  LDD1733 
Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) LDTP04437 6.87  LDD1733 
Aldehyde dehydrogenase, mitochondrial (ALDH2) LDTP02074 5.82  LDD1733 
Alpha-N-acetylglucosaminidase (NAGLU) LDTP04634 7.21  LDD1733 
Apoptosis-inducing factor 1, mitochondrial (AIFM1) LDTP01687 30.70  LDD1733 
ATP synthase membrane subunit K, mitochondrial (ATP5MK) LDTP10432 9.51  LDD1733 
ATP-dependent 6-phosphofructokinase, liver type (PFKL) LDTP02925 26.54  LDD1733 
ATP-dependent 6-phosphofructokinase, muscle type (PFKM) LDTP02261 19.16  LDD1733 
ATP-dependent 6-phosphofructokinase, platelet type (PFKP) LDTP05314 10.13  LDD1733 
ATP-dependent translocase ABCB1 (ABCB1) LDTP02254 7.62  LDD1733 
ATPase GET3 (GET3) LDTP00836 8.17  LDD1733 
Bifunctional epoxide hydrolase 2 (EPHX2) LDTP03692 21.86  LDD1733 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 6.54  LDD1733 
Carbohydrate sulfotransferase 14 (CHST14) LDTP09096 6.68  LDD1733 
Carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) LDTP04358 7.57  LDD1733 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 6.28  LDD1733 
Catechol O-methyltransferase (COMT) LDTP03145 7.89  LDD1733 
Ceramide synthase 2 (CERS2) LDTP10329 11.71  LDD1733 
Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1) LDTP11178 15.14  LDD1733 
Cytochrome b5 type B (CYB5B) LDTP00697 23.92  LDD1733 
Cytochrome c oxidase subunit 7A-related protein, mitochondrial (COX7A2L) LDTP14427 6.59  LDD1733 
DNA-directed RNA polymerase III subunit RPC6 (POLR3F) LDTP11762 8.75  LDD1733 
Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (ENPP1) LDTP03170 11.31  LDD1733 
Enoyl-CoA delta isomerase 1, mitochondrial (ECI1) LDTP03965 7.06  LDD1733 
Epimerase family protein SDR39U1 (SDR39U1) LDTP16070 12.04  LDD1733 
Epoxide hydrolase 1 (EPHX1) LDTP02175 10.27  LDD1733 
Estradiol 17-beta-dehydrogenase 11 (HSD17B11) LDTP09063 7.84  LDD1733 
Ferrochelatase, mitochondrial (FECH) LDTP03198 11.88  LDD1733 
Glutathione hydrolase 7 (GGT7) LDTP13367 8.17  LDD1733 
GPI-anchor transamidase (PIGK) LDTP09835 5.50  LDD1733 
Heme A synthase COX15 (COX15) LDTP07931 10.27  LDD1733 
Heme oxygenase 1 (HMOX1) LDTP02359 13.18  LDD1733 
Heme oxygenase 2 (HMOX2) LDTP03540 10.93  LDD1733 
Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) LDTP06787 6.87  LDD1733 
Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1) LDTP01333 5.74  LDD1733 
Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) LDTP04211 10.78  LDD1733 
Lanosterol 14-alpha demethylase (CYP51A1) LDTP06648 7.16  LDD1733 
Long-chain fatty acid transport protein 2 (SLC27A2) LDTP00496 7.84  LDD1733 
Long-chain-fatty-acid--CoA ligase 4 (ACSL4) LDTP00982 7.26  LDD1733 
Long-chain-fatty-acid--CoA ligase 6 (ACSL6) LDTP13486 7.26  LDD1733 
Macrophage migration inhibitory factor (MIF) LDTP02713 22.01  LDD1733 
Methylmalonyl-CoA mutase, mitochondrial (MMUT) LDTP03150 11.39  LDD1733 
Mitochondrial-processing peptidase subunit alpha (PMPCA) LDTP05632 12.04  LDD1733 
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) LDTP16258 5.58  LDD1733 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 5.46  LDD1733 
NADH dehydrogenase iron-sulfur protein 2, mitochondrial (NDUFS2) LDTP01168 5.43  LDD1733 
NADH dehydrogenase iron-sulfur protein 3, mitochondrial (NDUFS3) LDTP01238 11.63  LDD1733 
NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) LDTP01982 11.00  LDD1733 
Neutral cholesterol ester hydrolase 1 (NCEH1) LDTP07591 6.23  LDD1733 
Patatin-like phospholipase domain-containing protein 6 (PNPLA6) LDTP08617 5.98  LDD1733 
Peptidyl-prolyl cis-trans isomerase FKBP10 (FKBP10) LDTP10101 5.31  LDD1733 
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) LDTP14218 5.82  LDD1733 
Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3) LDTP09155 20.11  LDD1733 
Phosphatidylserine synthase 1 (PTDSS1) LDTP04203 7.52  LDD1733 
Prenylcysteine oxidase-like (PCYOX1L) LDTP15480 7.06  LDD1733 
Presequence protease, mitochondrial (PITRM1) LDTP07003 6.41  LDD1733 
Probable 18S rRNA (guanine-N(7))-methyltransferase (BUD23) LDTP00845 6.02  LDD1733 
Probable glutathione peroxidase 8 (GPX8) LDTP09495 6.23  LDD1733 
Prolyl endopeptidase-like (PREPL) LDTP06849 8.51  LDD1733 
Prostaglandin E synthase 2 (PTGES2) LDTP12040 6.77  LDD1733 
Prostaglandin reductase 3 (PTGR3) LDTP15422 7.16  LDD1733 
Protein disulfide-isomerase (P4HB) LDTP02188 6.63  LDD1733 
Protein disulfide-isomerase TMX3 (TMX3) LDTP10471 5.70  LDD1733 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 7.73  LDD1733 
Protoporphyrinogen oxidase (PPOX) LDTP04353 5.58  LDD1733 
Puromycin-sensitive aminopeptidase (NPEPPS) LDTP04712 7.01  LDD1733 
Ras-related protein R-Ras2 (RRAS2) LDTP04955 5.31  LDD1733 
Ras-related protein Rab-10 (RAB10) LDTP04895 5.35  LDD1733 
Ras-related protein Rab-5B (RAB5B) LDTP04893 5.66  LDD1733 
S-methyl-5'-thioadenosine phosphorylase (MTAP) LDTP05758 14.22  LDD1733 
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) LDTP02860 5.58  LDD1733 
Signal peptidase complex catalytic subunit SEC11A (SEC11A) LDTP05066 13.09  LDD1733 
Sphingosine-1-phosphate lyase 1 (SGPL1) LDTP01613 21.86  LDD1733 
Squalene monooxygenase (SQLE) LDTP06142 6.92  LDD1733 
Stearoyl-CoA desaturase (SCD) LDTP00344 7.11  LDD1733 
Tissue alpha-L-fucosidase (FUCA1) LDTP02006 6.54  LDD1733 
Tripeptidyl-peptidase 1 (TPP1) LDTP00428 8.11  LDD1733 
Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADVL) LDTP04289 5.78  LDD1733 
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⏷ Show the Full List of 84 Enzyme
39 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
ATP synthase F(0) complex subunit B1, mitochondrial (ATP5PB) LDTP03284 5.50  LDD1733 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 5.31  LDD1733 
Cytoskeleton-associated protein 4 (CKAP4) LDTP05511 5.54  LDD1733 
Equilibrative nucleoside transporter 1 (SLC29A1) LDTP10985 13.74  LDD1733 
ER membrane protein complex subunit 3 (EMC3) LDTP12958 5.86  LDD1733 
Erlin-1 (ERLIN1) LDTP01234 5.86  LDD1733 
Golgin subfamily A member 5 (GOLGA5) LDTP09333 5.28  LDD1733 
Lysosome membrane protein 2 (SCARB2) LDTP06034 9.19  LDD1733 
Major facilitator superfamily domain-containing protein 10 (MFSD10) LDTP06198 22.16  LDD1733 
Membrane-associated progesterone receptor component 2 (PGRMC2) LDTP00562 6.82  LDD1733 
Mitochondrial 2-oxodicarboxylate carrier (SLC25A21) LDTP11095 5.54  LDD1733 
Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) LDTP00865 6.06  LDD1733 
Mitochondrial carrier homolog 1 (MTCH1) LDTP12909 5.39  LDD1733 
Mitochondrial carrier homolog 2 (MTCH2) LDTP14235 5.58  LDD1733 
Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) LDTP13983 7.94  LDD1733 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 22.32  LDD1733 
Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) LDTP00477 7.11  LDD1733 
Mitochondrial inner membrane protein OXA1L (OXA1L) LDTP06308 8.63  LDD1733 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 9.32  LDD1733 
Mpv17-like protein 2 (MPV17L2) LDTP14952 12.64  LDD1733 
Peroxisomal membrane protein PEX14 (PEX14) LDTP01200 6.06  LDD1733 
PRA1 family protein 2 (PRAF2) LDTP01060 7.94  LDD1733 
PRA1 family protein 3 (ARL6IP5) LDTP01352 5.90  LDD1733 
Protein GPR107 (GPR107) LDTP07236 5.62  LDD1733 
Putative lipid scramblase CLPTM1 (CLPTM1) LDTP01745 6.63  LDD1733 
Rhomboid domain-containing protein 2 (RHBDD2) LDTP07467 7.84  LDD1733 
Scavenger receptor class B member 1 (SCARB1) LDTP09547 5.39  LDD1733 
Secretory carrier-associated membrane protein 3 (SCAMP3) LDTP00451 8.51  LDD1733 
Sideroflexin-4 (SFXN4) LDTP07531 8.63  LDD1733 
Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1) LDTP02544 6.73  LDD1733 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 7.06  LDD1733 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 5.62  LDD1733 
Transmembrane protein 126A (TMEM126A) LDTP15948 18.13  LDD1733 
Transmembrane protein 201 (TMEM201) LDTP07058 6.02  LDD1733 
Transmembrane protein 205 (TMEM205) LDTP07667 6.59  LDD1733 
Transmembrane protein 70, mitochondrial (TMEM70) LDTP11229 5.78  LDD1733 
Vezatin (VEZT) LDTP12191 6.77  LDD1733 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 6.02  LDD1733 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 5.74  LDD1733 
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⏷ Show the Full List of 39 Transporter and channel
41 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) LDTP08364 5.78  LDD1733 
Arfaptin-1 (ARFIP1) LDTP04558 5.31  LDD1733 
Bromodomain-containing protein 7 (BRD7) LDTP12369 10.06  LDD1733 
Centromere protein K (CENPK) LDTP11143 6.02  LDD1733 
Clustered mitochondria protein homolog (CLUH) LDTP14486 6.11  LDD1733 
COP9 signalosome complex subunit 8 (COPS8) LDTP10927 5.35  LDD1733 
Core histone macro-H2A.2 (MACROH2A2) LDTP12971 5.98  LDD1733 
Emerin (EMD) LDTP04356 7.94  LDD1733 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 8.69  LDD1733 
Golgi reassembly-stacking protein 2 (GORASP2) LDTP12073 5.28  LDD1733 
Heme-binding protein 1 (HEBP1) LDTP16078 6.28  LDD1733 
Kinetochore protein Spc24 (SPC24) LDTP09069 5.28  LDD1733 
Large ribosomal subunit protein mL46 (MRPL46) LDTP15976 6.54  LDD1733 
Membralin (TMEM259) LDTP14939 6.06  LDD1733 
MICOS complex subunit MIC27 (APOOL) LDTP07718 5.54  LDD1733 
Mitochondrial fission factor (MFF) LDTP11670 8.46  LDD1733 
Mitochondrial fission process protein 1 (MTFP1) LDTP16192 8.17  LDD1733 
Neuferricin (CYB5D2) LDTP15623 8.51  LDD1733 
Nucleobindin-2 (NUCB2) LDTP05160 18.00  LDD1733 
OCIA domain-containing protein 1 (OCIAD1) LDTP12755 5.50  LDD1733 
Peroxiredoxin-like 2A (PRXL2A) LDTP11140 6.45  LDD1733 
Protein CutA (CUTA) LDTP01075 32.45  LDD1733 
Protein FAM162A (FAM162A) LDTP10056 5.54  LDD1733 
Protein jagunal homolog 1 (JAGN1) LDTP08904 5.28  LDD1733 
Protein LYRIC (MTDH) LDTP08224 11.16  LDD1733 
Protein unc-119 homolog B (UNC119B) LDTP00105 6.02  LDD1733 
Protein YIF1A (YIF1A) LDTP01494 6.73  LDD1733 
Receptor expression-enhancing protein 4 (REEP4) LDTP11981 5.62  LDD1733 
Reticulon-2 (RTN2) LDTP01167 9.65  LDD1733 
Reticulon-3 (RTN3) LDTP01522 8.11  LDD1733 
Reticulon-4 (RTN4) LDTP12412 7.84  LDD1733 
Reticulophagy regulator 3 (RETREG3) LDTP08300 6.36  LDD1733 
RNA-binding protein EWS (EWSR1) LDTP05318 5.74  LDD1733 
Survival of motor neuron-related-splicing factor 30 (SMNDC1) LDTP01362 7.11  LDD1733 
Tetraspanin-6 (TSPAN6) LDTP14458 7.06  LDD1733 
Tetratricopeptide repeat protein 19, mitochondrial (TTC19) LDTP07368 5.24  LDD1733 
Thioredoxin-related transmembrane protein 4 (TMX4) LDTP18373 5.86  LDD1733 
TraB domain-containing protein (TRABD) LDTP19963 7.16  LDD1733 
Tudor and KH domain-containing protein (TDRKH) LDTP13922 5.39  LDD1733 
Ubiquilin-1 (UBQLN1) LDTP13626 5.39  LDD1733 
Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) LDTP15067 10.48  LDD1733 
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⏷ Show the Full List of 41 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD1733 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587