Details of the Target

General Information of Target

Target ID LDTP02261
Target Name ATP-dependent 6-phosphofructokinase, muscle type (PFKM)
Gene Name PFKM
Gene ID 5213
Synonyms
PFKX; ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; EC 2.7.1.11; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVD
GGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDG
SLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDS
ALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWE
EHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQ
RGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTK
AMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVR
STVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQ
ISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSV
GADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPF
TIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLG
HMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVF
QPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV
Target Bioclass
Enzyme
Family
Phosphofructokinase type A (PFKA) family, ATP-dependent PFK group I subfamily, Eukaryotic two domain clade 'E' sub-subfamily
Subcellular location
Cytoplasm
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Uniprot ID
P08237
Ensemble ID
ENST00000312352.11
HGNC ID
HGNC:8877
ChEMBL ID
CHEMBL3291

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL27 Substitution: p.Q447K DBIA    Probe Info 
HT115 SNV: p.V710G DBIA    Probe Info 
Ishikawa (Heraklio) 02 ER Deletion: p.F596LfsTer21 DBIA    Probe Info 
KYSE150 SNV: p.Q78Ter DBIA    Probe Info 
NCIH1299 Substitution: p.Q447K .
NCIH2170 Substitution: p.Q447K DBIA    Probe Info 
RKO SNV: p.C233R DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 28 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
TH211
 Probe Info  		Y455(7.54); Y644(6.34)  LDD0257  [2]
TH216
 Probe Info  		Y385(20.00); Y103(15.82)  LDD0259  [2]
ONAyne
 Probe Info  		K144(0.64)  LDD0274  [3]
DBIA
 Probe Info  		C185(1.84); C780(2.10)  LDD3310  [4]
P11
 Probe Info  		1.08  LDD0210  [5]
THZ1-DTB
 Probe Info  		C550(1.10)  LDD0460  [6]
BTD
 Probe Info  		C351(1.02)  LDD2091  [7]
AHL-Pu-1
 Probe Info  		C709(2.58)  LDD0170  [8]
EA-probe
 Probe Info  		C334(0.67)  LDD2210  [9]
HHS-482
 Probe Info  		Y103(0.57)  LDD0285  [10]
HHS-475
 Probe Info  		Y103(0.64)  LDD0264  [11]
HHS-465
 Probe Info  		Y103(10.00)  LDD2237  [12]
5E-2FA
 Probe Info  		H661(0.00); H401(0.00); H52(0.00)  LDD2235  [13]
ATP probe
 Probe Info  		K10(0.00); K738(0.00); K754(0.00); K727(0.00)  LDD0199  [14]
N1
 Probe Info  		N.A.  LDD0245  [15]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [16]
JW-RF-010
 Probe Info  		C709(0.00); C351(0.00)  LDD0026  [17]
NAIA_4
 Probe Info  		C170(0.00); C351(0.00); C631(0.00); C709(0.00)  LDD2226  [18]
TFBX
 Probe Info  		N.A.  LDD0027  [17]
IPM
 Probe Info  		C351(0.00); C709(0.00)  LDD0005  [19]
NHS
 Probe Info  		N.A.  LDD0010  [19]
STPyne
 Probe Info  		N.A.  LDD0009  [19]
VSF
 Probe Info  		N.A.  LDD0007  [19]
Acrolein
 Probe Info  		C114(0.00); C351(0.00); H390(0.00)  LDD0217  [20]
Methacrolein
 Probe Info  		C351(0.00); C709(0.00)  LDD0218  [20]
AOyne
 Probe Info  		9.60  LDD0443  [21]
NAIA_5
 Probe Info  		C709(0.00); C351(0.00); C631(0.00); C577(0.00)  LDD2223  [18]
PAL-AfBPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info  		19.16  LDD1733  [22]
FFF probe13
 Probe Info  		5.56  LDD0475  [23]
FFF probe14
 Probe Info  		12.43  LDD0477  [23]
FFF probe3
 Probe Info  		7.15  LDD0465  [23]
STS-2
 Probe Info  		N.A.  LDD0138  [24]
STS-1
 Probe Info  		N.A.  LDD0069  [25]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C709(2.58)  LDD0170  [8]
 LDCM0214  AC1 HEK-293T C351(1.02); C709(1.04); C114(0.95); C631(1.04)  LDD1507  [26]
 LDCM0215  AC10 HEK-293T C351(0.85); C709(1.02); C114(1.01); C631(1.08)  LDD1508  [26]
 LDCM0226  AC11 HEK-293T C351(0.92); C709(1.05); C114(0.96); C631(1.09)  LDD1509  [26]
 LDCM0237  AC12 HEK-293T C351(1.01); C709(0.99); C114(1.05); C631(1.10)  LDD1510  [26]
 LDCM0259  AC14 HEK-293T C351(0.97); C709(1.00); C114(0.99); C631(1.02)  LDD1512  [26]
 LDCM0270  AC15 HEK-293T C351(0.99); C709(1.06); C114(1.00); C631(1.09)  LDD1513  [26]
 LDCM0276  AC17 HEK-293T C351(1.09); C709(0.95); C114(0.95); C631(1.02)  LDD1515  [26]
 LDCM0277  AC18 HEK-293T C351(0.98); C709(0.98); C114(1.08); C631(1.06)  LDD1516  [26]
 LDCM0278  AC19 HEK-293T C351(1.30); C709(1.15); C114(0.92); C631(1.10)  LDD1517  [26]
 LDCM0279  AC2 HEK-293T C351(0.95); C709(0.97); C114(1.03); C631(1.07)  LDD1518  [26]
 LDCM0280  AC20 HEK-293T C351(0.94); C709(0.98); C114(1.10); C631(1.12)  LDD1519  [26]
 LDCM0281  AC21 HEK-293T C351(0.98); C709(1.03); C114(1.06); C631(1.01)  LDD1520  [26]
 LDCM0282  AC22 HEK-293T C351(0.96); C709(1.06); C114(0.91); C631(0.92)  LDD1521  [26]
 LDCM0283  AC23 HEK-293T C351(1.00); C709(0.97); C114(1.02); C631(1.02)  LDD1522  [26]
 LDCM0284  AC24 HEK-293T C351(1.01); C709(1.07); C114(1.14); C631(0.99)  LDD1523  [26]
 LDCM0285  AC25 HEK-293T C351(1.07); C709(0.97); C114(1.00); C631(1.05)  LDD1524  [26]
 LDCM0286  AC26 HEK-293T C351(1.00); C709(1.08); C114(1.02); C631(1.11)  LDD1525  [26]
 LDCM0287  AC27 HEK-293T C351(1.00); C709(1.05); C114(0.90); C631(0.99)  LDD1526  [26]
 LDCM0288  AC28 HEK-293T C351(0.97); C709(1.02); C114(1.00); C631(1.02)  LDD1527  [26]
 LDCM0289  AC29 HEK-293T C351(1.00); C709(1.02); C114(1.03); C631(1.05)  LDD1528  [26]
 LDCM0290  AC3 HEK-293T C351(0.98); C709(1.02); C114(0.96); C631(1.03)  LDD1529  [26]
 LDCM0291  AC30 HEK-293T C351(0.99); C709(1.05); C114(0.95); C631(0.93)  LDD1530  [26]
 LDCM0292  AC31 HEK-293T C351(1.07); C709(1.04); C114(1.00); C631(1.03)  LDD1531  [26]
 LDCM0293  AC32 HEK-293T C351(1.04); C709(0.90); C114(1.11); C631(1.02)  LDD1532  [26]
 LDCM0294  AC33 HEK-293T C351(1.01); C709(1.01); C114(0.95); C631(1.01)  LDD1533  [26]
 LDCM0295  AC34 HEK-293T C351(0.94); C709(1.06); C114(1.03); C631(0.96)  LDD1534  [26]
 LDCM0296  AC35 HEK-293T C351(0.94); C709(0.99); C114(0.89); C631(1.00)  LDD1535  [26]
 LDCM0297  AC36 HEK-293T C351(0.96); C709(1.05); C114(0.94); C631(1.00)  LDD1536  [26]
 LDCM0298  AC37 HEK-293T C351(0.93); C709(0.98); C114(1.02); C631(1.01)  LDD1537  [26]
 LDCM0299  AC38 HEK-293T C351(0.94); C709(1.04); C114(0.90); C631(0.98)  LDD1538  [26]
 LDCM0300  AC39 HEK-293T C351(1.00); C709(1.04); C114(1.10); C631(1.04)  LDD1539  [26]
 LDCM0301  AC4 HEK-293T C351(0.91); C709(0.97); C114(0.98); C631(0.96)  LDD1540  [26]
 LDCM0302  AC40 HEK-293T C351(0.91); C709(1.02); C114(1.07); C631(0.97)  LDD1541  [26]
 LDCM0303  AC41 HEK-293T C351(1.02); C709(1.02); C114(0.98); C631(1.04)  LDD1542  [26]
 LDCM0304  AC42 HEK-293T C351(0.96); C709(1.07); C114(1.05); C631(1.09)  LDD1543  [26]
 LDCM0305  AC43 HEK-293T C351(0.96); C709(1.08); C114(0.99); C631(1.00)  LDD1544  [26]
 LDCM0306  AC44 HEK-293T C351(0.98); C709(1.12); C114(1.12); C631(1.04)  LDD1545  [26]
 LDCM0307  AC45 HEK-293T C351(0.94); C709(1.02); C114(1.04); C631(1.04)  LDD1546  [26]
 LDCM0308  AC46 HEK-293T C351(0.96); C709(1.06); C114(1.00); C631(1.04)  LDD1547  [26]
 LDCM0309  AC47 HEK-293T C351(0.98); C709(1.05); C114(1.14); C631(1.07)  LDD1548  [26]
 LDCM0310  AC48 HEK-293T C351(1.03); C709(1.04); C114(1.03); C631(1.04)  LDD1549  [26]
 LDCM0311  AC49 HEK-293T C351(1.02); C709(0.95); C114(0.99); C631(1.01)  LDD1550  [26]
 LDCM0312  AC5 HEK-293T C351(0.97); C709(0.96); C114(1.12); C631(1.01)  LDD1551  [26]
 LDCM0313  AC50 HEK-293T C351(0.93); C709(1.02); C114(1.06); C631(1.09)  LDD1552  [26]
 LDCM0314  AC51 HEK-293T C351(0.93); C709(1.07); C114(0.88); C631(0.99)  LDD1553  [26]
 LDCM0315  AC52 HEK-293T C351(0.98); C709(1.05); C114(1.05); C631(1.04)  LDD1554  [26]
 LDCM0316  AC53 HEK-293T C351(0.98); C709(1.07); C114(1.11); C631(1.12)  LDD1555  [26]
 LDCM0317  AC54 HEK-293T C351(0.95); C709(1.12); C114(1.02); C631(0.95)  LDD1556  [26]
 LDCM0318  AC55 HEK-293T C351(1.01); C709(1.01); C114(1.00); C631(1.16)  LDD1557  [26]
 LDCM0319  AC56 HEK-293T C351(0.83); C709(1.04); C114(1.14); C631(0.99)  LDD1558  [26]
 LDCM0320  AC57 HEK-293T C351(1.01); C709(1.06); C114(1.05); C631(0.98)  LDD1559  [26]
 LDCM0321  AC58 HEK-293T C351(0.94); C709(1.04); C114(0.97); C631(1.05)  LDD1560  [26]
 LDCM0322  AC59 HEK-293T C351(0.94); C709(1.01); C114(0.93); C631(1.04)  LDD1561  [26]
 LDCM0323  AC6 HEK-293T C351(0.99); C709(1.10); C114(1.08); C631(0.96)  LDD1562  [26]
 LDCM0324  AC60 HEK-293T C351(0.98); C709(1.03); C114(1.01); C631(1.03)  LDD1563  [26]
 LDCM0325  AC61 HEK-293T C351(1.01); C709(0.98); C114(1.03); C631(0.98)  LDD1564  [26]
 LDCM0326  AC62 HEK-293T C351(0.95); C709(0.99); C114(0.96); C631(0.97)  LDD1565  [26]
 LDCM0327  AC63 HEK-293T C351(1.03); C709(0.96); C114(1.07); C631(1.05)  LDD1566  [26]
 LDCM0328  AC64 HEK-293T C351(0.95); C709(1.01); C114(1.00); C631(1.04)  LDD1567  [26]
 LDCM0334  AC7 HEK-293T C351(0.97); C709(0.96); C114(1.05); C631(1.03)  LDD1568  [26]
 LDCM0345  AC8 HEK-293T C351(1.00); C709(0.94); C114(1.10); C631(0.90)  LDD1569  [26]
 LDCM0248  AKOS034007472 HEK-293T C351(1.01); C709(0.98); C114(1.01); C631(1.00)  LDD1511  [26]
 LDCM0356  AKOS034007680 HEK-293T C351(1.00); C709(0.96); C114(1.07); C631(1.03)  LDD1570  [26]
 LDCM0275  AKOS034007705 HEK-293T C351(0.95); C709(1.14); C114(0.94); C631(1.02)  LDD1514  [26]
 LDCM0498  BS-3668 MDA-MB-231 C351(1.02)  LDD2091  [7]
 LDCM0089  C30 HEK-293T 1.08  LDD0210  [5]
 LDCM0108  Chloroacetamide HeLa C114(0.00); C351(0.00); C709(0.00); H298(0.00)  LDD0222  [20]
 LDCM0632  CL-Sc Hep-G2 C351(1.05)  LDD2227  [18]
 LDCM0367  CL1 HEK-293T C351(0.98); C709(1.10); C114(0.89); C631(1.44)  LDD1571  [26]
 LDCM0368  CL10 HEK-293T C351(0.98); C709(1.14); C114(1.00); C631(1.07)  LDD1572  [26]
 LDCM0369  CL100 HEK-293T C351(0.99); C709(1.00); C114(0.90); C631(0.95)  LDD1573  [26]
 LDCM0370  CL101 HEK-293T C351(0.94); C709(0.96); C114(0.84); C631(1.21)  LDD1574  [26]
 LDCM0371  CL102 HEK-293T C351(0.94); C709(1.07); C114(0.95); C631(1.08)  LDD1575  [26]
 LDCM0372  CL103 HEK-293T C351(0.91); C709(1.03); C114(0.99); C631(1.04)  LDD1576  [26]
 LDCM0373  CL104 HEK-293T C351(1.01); C709(0.96); C114(1.00); C631(1.03)  LDD1577  [26]
 LDCM0374  CL105 HEK-293T C351(0.93); C709(0.95); C114(0.92); C631(1.21)  LDD1578  [26]
 LDCM0375  CL106 HEK-293T C351(0.98); C709(1.01); C114(0.94); C631(0.98)  LDD1579  [26]
 LDCM0376  CL107 HEK-293T C351(0.96); C709(1.00); C114(0.92); C631(1.03)  LDD1580  [26]
 LDCM0377  CL108 HEK-293T C351(1.01); C709(0.95); C114(0.99); C631(1.04)  LDD1581  [26]
 LDCM0378  CL109 HEK-293T C351(0.94); C709(0.91); C114(0.92); C631(0.91)  LDD1582  [26]
 LDCM0379  CL11 HEK-293T C351(0.97); C709(1.10); C114(1.02); C631(1.10)  LDD1583  [26]
 LDCM0380  CL110 HEK-293T C351(0.94); C709(1.13); C114(0.95); C631(0.97)  LDD1584  [26]
 LDCM0381  CL111 HEK-293T C351(0.94); C709(1.01); C114(1.03); C631(1.00)  LDD1585  [26]
 LDCM0382  CL112 HEK-293T C351(1.00); C709(1.00); C114(1.02); C631(0.99)  LDD1586  [26]
 LDCM0383  CL113 HEK-293T C351(0.91); C709(0.95); C114(0.92); C631(1.42)  LDD1587  [26]
 LDCM0384  CL114 HEK-293T C351(0.97); C709(1.06); C114(0.87); C631(1.13)  LDD1588  [26]
 LDCM0385  CL115 HEK-293T C351(0.94); C709(1.01); C114(1.00); C631(1.09)  LDD1589  [26]
 LDCM0386  CL116 HEK-293T C351(0.99); C709(1.01); C114(0.94); C631(0.97)  LDD1590  [26]
 LDCM0387  CL117 HEK-293T C351(0.93); C709(0.91); C114(0.97); C631(1.35)  LDD1591  [26]
 LDCM0388  CL118 HEK-293T C351(0.92); C709(1.05); C114(0.88); C631(1.14)  LDD1592  [26]
 LDCM0389  CL119 HEK-293T C351(0.93); C709(1.04); C114(0.99); C631(1.08)  LDD1593  [26]
 LDCM0390  CL12 HEK-293T C351(0.95); C709(1.33); C114(1.09); C631(1.12)  LDD1594  [26]
 LDCM0391  CL120 HEK-293T C351(1.05); C709(0.95); C114(1.06); C631(1.03)  LDD1595  [26]
 LDCM0392  CL121 HEK-293T C351(0.96); C709(0.91); C114(0.94); C631(1.30)  LDD1596  [26]
 LDCM0393  CL122 HEK-293T C351(0.91); C709(1.08); C114(0.97); C631(1.14)  LDD1597  [26]
 LDCM0394  CL123 HEK-293T C351(0.93); C709(1.05); C114(1.12); C631(1.00)  LDD1598  [26]
 LDCM0395  CL124 HEK-293T C351(0.97); C709(1.10); C114(0.98); C631(1.04)  LDD1599  [26]
 LDCM0396  CL125 HEK-293T C351(0.96); C709(0.95); C114(1.01); C631(1.18)  LDD1600  [26]
 LDCM0397  CL126 HEK-293T C351(0.98); C709(1.12); C114(0.96); C631(0.94)  LDD1601  [26]
 LDCM0398  CL127 HEK-293T C351(1.00); C709(1.04); C114(0.96); C631(0.99)  LDD1602  [26]
 LDCM0399  CL128 HEK-293T C351(1.02); C709(1.06); C114(1.02); C631(0.98)  LDD1603  [26]
 LDCM0400  CL13 HEK-293T C351(1.01); C709(0.95); C114(0.96); C631(1.52)  LDD1604  [26]
 LDCM0401  CL14 HEK-293T C351(0.97); C709(1.01); C114(1.00); C631(1.14)  LDD1605  [26]
 LDCM0402  CL15 HEK-293T C351(0.96); C709(1.10); C114(0.96); C631(1.06)  LDD1606  [26]
 LDCM0403  CL16 HEK-293T C351(1.06); C709(0.90); C114(1.10); C631(0.98)  LDD1607  [26]
 LDCM0404  CL17 HEK-293T C351(0.96); C709(1.18); C114(1.16); C631(1.06)  LDD1608  [26]
 LDCM0405  CL18 HEK-293T C351(0.91); C709(1.19); C114(1.09); C631(1.22)  LDD1609  [26]
 LDCM0406  CL19 HEK-293T C351(0.98); C709(1.17); C114(1.06); C631(1.09)  LDD1610  [26]
 LDCM0407  CL2 HEK-293T C351(0.99); C709(1.04); C114(0.97); C631(0.95)  LDD1611  [26]
 LDCM0408  CL20 HEK-293T C351(0.97); C709(1.24); C114(1.18); C631(1.06)  LDD1612  [26]
 LDCM0409  CL21 HEK-293T C351(0.95); C709(1.33); C114(1.17); C631(0.95)  LDD1613  [26]
 LDCM0410  CL22 HEK-293T C351(0.97); C709(1.22); C114(1.05); C631(1.10)  LDD1614  [26]
 LDCM0411  CL23 HEK-293T C351(0.99); C709(1.03); C114(1.18); C631(1.17)  LDD1615  [26]
 LDCM0412  CL24 HEK-293T C351(0.95); C709(1.45); C114(1.27); C631(1.09)  LDD1616  [26]
 LDCM0413  CL25 HEK-293T C351(0.99); C709(0.96); C114(0.89); C631(1.46)  LDD1617  [26]
 LDCM0414  CL26 HEK-293T C351(0.97); C709(1.10); C114(0.84); C631(0.97)  LDD1618  [26]
 LDCM0415  CL27 HEK-293T C351(0.97); C709(1.00); C114(0.89); C631(1.06)  LDD1619  [26]
 LDCM0416  CL28 HEK-293T C351(1.02); C709(1.00); C114(1.01); C631(1.07)  LDD1620  [26]
 LDCM0417  CL29 HEK-293T C351(1.03); C709(1.01); C114(0.95); C631(1.10)  LDD1621  [26]
 LDCM0418  CL3 HEK-293T C351(0.94); C709(1.05); C114(0.99); C631(1.03)  LDD1622  [26]
 LDCM0419  CL30 HEK-293T C351(0.90); C709(1.05); C114(1.09); C631(1.17)  LDD1623  [26]
 LDCM0420  CL31 HEK-293T C351(0.94); C709(0.99); C114(0.91); C631(1.15)  LDD1624  [26]
 LDCM0421  CL32 HEK-293T C351(0.91); C709(1.17); C114(1.06); C631(1.15)  LDD1625  [26]
 LDCM0422  CL33 HEK-293T C351(0.92); C709(1.27); C114(1.02); C631(1.10)  LDD1626  [26]
 LDCM0423  CL34 HEK-293T C351(0.96); C709(1.10); C114(1.00); C631(1.05)  LDD1627  [26]
 LDCM0424  CL35 HEK-293T C351(0.99); C709(0.97); C114(1.09); C631(1.11)  LDD1628  [26]
 LDCM0425  CL36 HEK-293T C351(0.90); C709(1.28); C114(1.25); C631(1.19)  LDD1629  [26]
 LDCM0426  CL37 HEK-293T C351(0.92); C709(0.98); C114(0.93); C631(1.00)  LDD1630  [26]
 LDCM0428  CL39 HEK-293T C351(0.91); C709(1.04); C114(0.95); C631(1.19)  LDD1632  [26]
 LDCM0429  CL4 HEK-293T C351(1.08); C709(1.03); C114(1.08); C631(0.94)  LDD1633  [26]
 LDCM0430  CL40 HEK-293T C351(1.02); C709(1.03); C114(1.05); C631(0.93)  LDD1634  [26]
 LDCM0431  CL41 HEK-293T C351(0.97); C709(1.11); C114(1.03); C631(0.99)  LDD1635  [26]
 LDCM0432  CL42 HEK-293T C351(0.86); C709(1.05); C114(1.07); C631(1.10)  LDD1636  [26]
 LDCM0433  CL43 HEK-293T C351(0.87); C709(1.00); C114(1.00); C631(1.03)  LDD1637  [26]
 LDCM0434  CL44 HEK-293T C351(1.02); C709(1.19); C114(1.06); C631(1.07)  LDD1638  [26]
 LDCM0435  CL45 HEK-293T C351(0.89); C709(1.26); C114(1.31); C631(1.08)  LDD1639  [26]
 LDCM0436  CL46 HEK-293T C351(0.93); C709(1.13); C114(1.05); C631(1.06)  LDD1640  [26]
 LDCM0437  CL47 HEK-293T C351(1.07); C709(1.10); C114(0.99); C631(1.17)  LDD1641  [26]
 LDCM0438  CL48 HEK-293T C351(0.99); C709(1.37); C114(1.12); C631(1.13)  LDD1642  [26]
 LDCM0439  CL49 HEK-293T C351(0.92); C709(0.96); C114(0.90); C631(1.22)  LDD1643  [26]
 LDCM0440  CL5 HEK-293T C351(1.03); C709(1.14); C114(1.05); C631(1.04)  LDD1644  [26]
 LDCM0441  CL50 HEK-293T C351(0.93); C709(1.09); C114(0.90); C631(1.02)  LDD1645  [26]
 LDCM0443  CL52 HEK-293T C351(1.06); C709(1.08); C114(0.89); C631(0.97)  LDD1646  [26]
 LDCM0444  CL53 HEK-293T C351(1.06); C709(1.13); C114(0.96); C631(1.07)  LDD1647  [26]
 LDCM0445  CL54 HEK-293T C351(0.83); C709(1.22); C114(1.10); C631(1.13)  LDD1648  [26]
 LDCM0446  CL55 HEK-293T C351(0.85); C709(1.07); C114(0.99); C631(1.05)  LDD1649  [26]
 LDCM0447  CL56 HEK-293T C351(0.92); C709(1.28); C114(0.98); C631(1.17)  LDD1650  [26]
 LDCM0448  CL57 HEK-293T C351(0.93); C709(1.23); C114(1.15); C631(1.25)  LDD1651  [26]
 LDCM0449  CL58 HEK-293T C351(0.92); C709(1.04); C114(1.04); C631(1.24)  LDD1652  [26]
 LDCM0450  CL59 HEK-293T C351(0.96); C709(1.01); C114(1.17); C631(1.09)  LDD1653  [26]
 LDCM0451  CL6 HEK-293T C351(0.93); C709(1.17); C114(1.12); C631(1.11)  LDD1654  [26]
 LDCM0452  CL60 HEK-293T C351(0.93); C709(1.36); C114(1.10); C631(0.91)  LDD1655  [26]
 LDCM0453  CL61 HEK-293T C351(0.91); C709(0.93); C114(1.14); C631(1.03)  LDD1656  [26]
 LDCM0454  CL62 HEK-293T C351(0.97); C709(1.11); C114(0.91); C631(1.02)  LDD1657  [26]
 LDCM0455  CL63 HEK-293T C351(0.93); C709(1.04); C114(1.03); C631(1.09)  LDD1658  [26]
 LDCM0456  CL64 HEK-293T C351(1.00); C709(1.21); C114(1.00); C631(1.02)  LDD1659  [26]
 LDCM0457  CL65 HEK-293T C351(0.98); C709(1.00); C114(0.97); C631(1.06)  LDD1660  [26]
 LDCM0458  CL66 HEK-293T C351(0.91); C709(1.14); C114(1.09); C631(1.08)  LDD1661  [26]
 LDCM0459  CL67 HEK-293T C351(0.93); C709(1.08); C114(1.00); C631(0.98)  LDD1662  [26]
 LDCM0460  CL68 HEK-293T C351(0.92); C709(1.26); C114(1.13); C631(1.11)  LDD1663  [26]
 LDCM0461  CL69 HEK-293T C351(0.98); C709(1.22); C114(1.11); C631(1.11)  LDD1664  [26]
 LDCM0462  CL7 HEK-293T C351(0.91); C709(1.15); C114(1.02); C631(0.93)  LDD1665  [26]
 LDCM0463  CL70 HEK-293T C351(0.92); C709(1.06); C114(1.02); C631(1.15)  LDD1666  [26]
 LDCM0464  CL71 HEK-293T C351(0.95); C709(1.11); C114(1.01); C631(1.11)  LDD1667  [26]
 LDCM0465  CL72 HEK-293T C351(0.96); C709(1.25); C114(1.08); C631(1.13)  LDD1668  [26]
 LDCM0466  CL73 HEK-293T C351(0.87); C709(1.04); C114(1.11); C631(0.98)  LDD1669  [26]
 LDCM0467  CL74 HEK-293T C351(0.94); C709(1.06); C114(0.92); C631(1.16)  LDD1670  [26]
 LDCM0469  CL76 HEK-293T C351(1.02); C709(0.98); C114(0.95); C631(1.01)  LDD1672  [26]
 LDCM0470  CL77 HEK-293T C351(0.93); C709(1.09); C114(0.98); C631(1.03)  LDD1673  [26]
 LDCM0471  CL78 HEK-293T C351(0.90); C709(1.12); C114(1.10); C631(1.04)  LDD1674  [26]
 LDCM0472  CL79 HEK-293T C351(0.92); C709(1.08); C114(1.01); C631(1.06)  LDD1675  [26]
 LDCM0473  CL8 HEK-293T C351(0.80); C709(1.29); C114(1.20); C631(1.00)  LDD1676  [26]
 LDCM0474  CL80 HEK-293T C351(0.94); C709(1.19); C114(1.11); C631(1.06)  LDD1677  [26]
 LDCM0475  CL81 HEK-293T C351(0.98); C709(1.29); C114(1.21); C631(1.07)  LDD1678  [26]
 LDCM0476  CL82 HEK-293T C351(0.95); C709(1.19); C114(0.96); C631(0.99)  LDD1679  [26]
 LDCM0477  CL83 HEK-293T C351(0.96); C709(1.05); C114(1.14); C631(1.07)  LDD1680  [26]
 LDCM0478  CL84 HEK-293T C351(0.98); C709(1.23); C114(1.13); C631(1.08)  LDD1681  [26]
 LDCM0479  CL85 HEK-293T C351(0.93); C709(1.04); C114(0.97); C631(1.02)  LDD1682  [26]
 LDCM0480  CL86 HEK-293T C351(0.99); C709(1.07); C114(1.00); C631(1.02)  LDD1683  [26]
 LDCM0481  CL87 HEK-293T C351(0.92); C709(1.06); C114(1.01); C631(1.13)  LDD1684  [26]
 LDCM0482  CL88 HEK-293T C351(1.12); C709(1.06); C114(1.10); C631(0.97)  LDD1685  [26]
 LDCM0483  CL89 HEK-293T C351(0.99); C709(1.11); C114(1.13); C631(1.03)  LDD1686  [26]
 LDCM0484  CL9 HEK-293T C351(0.95); C709(1.31); C114(1.16); C631(1.13)  LDD1687  [26]
 LDCM0485  CL90 HEK-293T C351(0.93); C709(1.05); C114(0.91); C631(1.05)  LDD1688  [26]
 LDCM0486  CL91 HEK-293T C351(0.85); C709(1.08); C114(1.01); C631(1.06)  LDD1689  [26]
 LDCM0487  CL92 HEK-293T C351(0.96); C709(1.25); C114(1.05); C631(0.99)  LDD1690  [26]
 LDCM0488  CL93 HEK-293T C351(0.91); C709(1.36); C114(1.09); C631(1.06)  LDD1691  [26]
 LDCM0489  CL94 HEK-293T C351(0.93); C709(1.18); C114(1.00); C631(0.92)  LDD1692  [26]
 LDCM0490  CL95 HEK-293T C351(1.15); C709(1.07); C114(0.89); C631(1.15)  LDD1693  [26]
 LDCM0491  CL96 HEK-293T C351(0.92); C709(1.28); C114(1.02); C631(1.08)  LDD1694  [26]
 LDCM0492  CL97 HEK-293T C351(0.94); C709(0.92); C114(0.84); C631(1.13)  LDD1695  [26]
 LDCM0493  CL98 HEK-293T C351(0.93); C709(1.01); C114(0.92); C631(1.03)  LDD1696  [26]
 LDCM0494  CL99 HEK-293T C351(0.90); C709(0.99); C114(0.92); C631(1.08)  LDD1697  [26]
 LDCM0495  E2913 HEK-293T C351(0.87); C709(1.01); C114(0.94); C631(1.07)  LDD1698  [26]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C351(1.27)  LDD1702  [7]
 LDCM0175  Ethacrynic acid HeLa C334(0.67)  LDD2210  [9]
 LDCM0625  F8 Ramos C351(1.08); C709(1.23); C89(7.04); C114(0.91)  LDD2187  [27]
 LDCM0572  Fragment10 Ramos C351(0.45); C709(1.50); C114(0.60)  LDD2189  [27]
 LDCM0573  Fragment11 Ramos C351(0.84); C709(0.39); C89(0.98); C114(0.38)  LDD2190  [27]
 LDCM0574  Fragment12 Ramos C351(1.45); C709(2.15); C114(0.83)  LDD2191  [27]
 LDCM0575  Fragment13 Ramos C351(0.91); C709(1.07); C114(0.63)  LDD2192  [27]
 LDCM0576  Fragment14 Ramos C351(1.36); C709(1.30); C89(1.49); C114(1.11)  LDD2193  [27]
 LDCM0579  Fragment20 Ramos C351(1.44); C709(1.53); C114(0.96)  LDD2194  [27]
 LDCM0580  Fragment21 Ramos C351(1.00); C709(1.09); C114(0.85)  LDD2195  [27]
 LDCM0582  Fragment23 Ramos C351(0.68); C709(0.84); C114(0.65)  LDD2196  [27]
 LDCM0578  Fragment27 Ramos C351(1.10); C709(0.79); C114(1.02)  LDD2197  [27]
 LDCM0586  Fragment28 Ramos C351(1.11); C709(1.22); C89(1.46); C114(0.78)  LDD2198  [27]
 LDCM0588  Fragment30 Ramos C351(0.93); C709(1.31); C114(0.74)  LDD2199  [27]
 LDCM0589  Fragment31 Ramos C351(1.27); C709(1.12); C114(0.15)  LDD2200  [27]
 LDCM0590  Fragment32 Ramos C351(1.05); C709(1.80); C114(0.56)  LDD2201  [27]
 LDCM0468  Fragment33 HEK-293T C351(0.88); C709(1.03); C114(0.96); C631(1.11)  LDD1671  [26]
 LDCM0596  Fragment38 Ramos C351(0.94); C709(1.08); C114(0.58)  LDD2203  [27]
 LDCM0566  Fragment4 Ramos C351(1.77); C709(2.96); C89(1.89); C114(0.89)  LDD2184  [27]
 LDCM0427  Fragment51 HEK-293T C351(0.98); C709(1.11); C114(0.93); C631(0.95)  LDD1631  [26]
 LDCM0610  Fragment52 Ramos C351(1.09); C709(1.36); C114(0.05)  LDD2204  [27]
 LDCM0614  Fragment56 Ramos C351(0.99); C709(1.06); C114(0.69)  LDD2205  [27]
 LDCM0569  Fragment7 Ramos C351(1.35); C709(2.36); C89(1.22); C114(0.69)  LDD2186  [27]
 LDCM0571  Fragment9 Ramos C351(0.72); C709(1.76); C114(0.75)  LDD2188  [27]
 LDCM0116  HHS-0101 DM93 Y103(0.64)  LDD0264  [11]
 LDCM0117  HHS-0201 DM93 Y103(0.67)  LDD0265  [11]
 LDCM0118  HHS-0301 DM93 Y103(0.70)  LDD0266  [11]
 LDCM0119  HHS-0401 DM93 Y103(0.67)  LDD0267  [11]
 LDCM0120  HHS-0701 DM93 Y103(0.62)  LDD0268  [11]
 LDCM0015  HNE MDA-MB-231 C709(0.91)  LDD0346  [27]
 LDCM0107  IAA HeLa H199(0.00); H52(0.00); C114(0.00); H298(0.00)  LDD0221  [20]
 LDCM0123  JWB131 DM93 Y103(0.57)  LDD0285  [10]
 LDCM0124  JWB142 DM93 Y103(0.93)  LDD0286  [10]
 LDCM0125  JWB146 DM93 Y103(0.74)  LDD0287  [10]
 LDCM0126  JWB150 DM93 Y103(1.41)  LDD0288  [10]
 LDCM0127  JWB152 DM93 Y103(2.59)  LDD0289  [10]
 LDCM0128  JWB198 DM93 Y103(3.28)  LDD0290  [10]
 LDCM0129  JWB202 DM93 Y103(1.12)  LDD0291  [10]
 LDCM0130  JWB211 DM93 Y103(1.25)  LDD0292  [10]
 LDCM0022  KB02 HEK-293T C709(1.00); C114(0.75); C351(0.91); C631(0.91)  LDD1492  [26]
 LDCM0023  KB03 HEK-293T C709(0.98); C114(0.84); C351(0.99); C631(0.83)  LDD1497  [26]
 LDCM0024  KB05 COLO792 C185(1.84); C780(2.10)  LDD3310  [4]
 LDCM0109  NEM HeLa H199(0.00); H611(0.00); H734(0.00); H661(0.00)  LDD0223  [20]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C351(0.99)  LDD2099  [7]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C351(1.42)  LDD2101  [7]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C709(1.10)  LDD2107  [7]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C351(0.62)  LDD2122  [7]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C351(0.91)  LDD2123  [7]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C351(0.72)  LDD2126  [7]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C351(0.63)  LDD2127  [7]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C351(1.41)  LDD2135  [7]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C351(1.10)  LDD2137  [7]
 LDCM0627  NUDT7-COV-1 HEK-293T C351(0.41)  LDD2206  [28]
 LDCM0628  OTUB2-COV-1 HEK-293T C351(0.40)  LDD2207  [28]
 LDCM0021  THZ1 HeLa S3 C550(1.10)  LDD0460  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATP-dependent 6-phosphofructokinase, liver type (PFKL) Phosphofructokinase type A (PFKA) family P17858
ATP-dependent 6-phosphofructokinase, muscle type (PFKM) Phosphofructokinase type A (PFKA) family P08237
E3 ubiquitin-protein ligase SIAH1 (SIAH1) SINA (Seven in absentia) family Q8IUQ4

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Discovery of Potent and Selective Inhibitors against Protein-Derived Electrophilic Cofactors. J Am Chem Soc. 2022 Mar 30;144(12):5377-5388. doi: 10.1021/jacs.1c12748. Epub 2022 Mar 2.
6 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
7 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
8 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
9 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
10 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
11 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
12 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
13 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
14 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
15 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
16 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
17 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
18 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
19 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
20 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
21 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
22 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
23 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
24 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
25 Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe. Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.
26 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
27 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
28 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.