Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C027  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 84 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01687  Apoptosis-inducing factor 1, mitochondrial (AIFM1) 30.7
 LDTP02925  ATP-dependent 6-phosphofructokinase, liver type (PFKL) 26.5
 LDTP00697  Cytochrome b5 type B (CYB5B) 23.9
 LDTP02713  Macrophage migration inhibitory factor (MIF) 22.0
 LDTP03692  Bifunctional epoxide hydrolase 2 (EPHX2) 21.9
 LDTP01613  Sphingosine-1-phosphate lyase 1 (SGPL1) 21.9
 LDTP09155  Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3) 20.1
 LDTP02261  ATP-dependent 6-phosphofructokinase, muscle type (PFKM) 19.2
 LDTP11178  Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1) 15.1
 LDTP05758  S-methyl-5'-thioadenosine phosphorylase (MTAP) 14.2
 LDTP02359  Heme oxygenase 1 (HMOX1) 13.2
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 13.1
 LDTP16070  Epimerase family protein SDR39U1 (SDR39U1) 12.0
 LDTP05632  Mitochondrial-processing peptidase subunit alpha (PMPCA) 12.0
 LDTP12293  Adipocyte plasma membrane-associated protein (APMAP) 11.9
 LDTP03198  Ferrochelatase, mitochondrial (FECH) 11.9
 LDTP11127  ADP-dependent glucokinase (ADPGK) 11.8
 LDTP10329  Ceramide synthase 2 (CERS2) 11.7
 LDTP01238  NADH dehydrogenase iron-sulfur protein 3, mitochondrial (NDUFS3) 11.6
 LDTP03150  Methylmalonyl-CoA mutase, mitochondrial (MMUT) 11.4
 LDTP03170  Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (ENPP1) 11.3
 LDTP01982  NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) 11.0
 LDTP03540  Heme oxygenase 2 (HMOX2) 10.9
 LDTP04211  Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) 10.8
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 10.3
 LDTP07931  Heme A synthase COX15 (COX15) 10.3
 LDTP05314  ATP-dependent 6-phosphofructokinase, platelet type (PFKP) 10.1
 LDTP10432  ATP synthase membrane subunit K, mitochondrial (ATP5MK) 9.5
 LDTP04196  26S proteasome non-ATPase regulatory subunit 8 (PSMD8) 8.8
 LDTP11762  DNA-directed RNA polymerase III subunit RPC6 (POLR3F) 8.8
 LDTP06849  Prolyl endopeptidase-like (PREPL) 8.5
 LDTP00836  ATPase GET3 (GET3) 8.2
 LDTP13367  Glutathione hydrolase 7 (GGT7) 8.2
 LDTP00428  Tripeptidyl-peptidase 1 (TPP1) 8.1
 LDTP03145  Catechol O-methyltransferase (COMT) 7.9
 LDTP09063  Estradiol 17-beta-dehydrogenase 11 (HSD17B11) 7.8
 LDTP00496  Long-chain fatty acid transport protein 2 (SLC27A2) 7.8
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 7.7
 LDTP04689  Adenosine kinase (ADK) 7.7
 LDTP08476  5'-3' exonuclease PLD3 (PLD3) 7.6
 LDTP02254  ATP-dependent translocase ABCB1 (ABCB1) 7.6
 LDTP04358  Carnitine O-palmitoyltransferase 1, liver isoform (CPT1A) 7.6
 LDTP04203  Phosphatidylserine synthase 1 (PTDSS1) 7.5
 LDTP00982  Long-chain-fatty-acid--CoA ligase 4 (ACSL4) 7.3
 LDTP13486  Long-chain-fatty-acid--CoA ligase 6 (ACSL6) 7.3
 LDTP04634  Alpha-N-acetylglucosaminidase (NAGLU) 7.2
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 7.2
 LDTP15422  Prostaglandin reductase 3 (PTGR3) 7.2
 LDTP00344  Stearoyl-CoA desaturase (SCD) 7.1
 LDTP03965  Enoyl-CoA delta isomerase 1, mitochondrial (ECI1) 7.1
 LDTP15480  Prenylcysteine oxidase-like (PCYOX1L) 7.1
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 7.0
 LDTP06142  Squalene monooxygenase (SQLE) 6.9
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 6.9
 LDTP06787  Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) 6.9
 LDTP12040  Prostaglandin E synthase 2 (PTGES2) 6.8
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 6.7
 LDTP02188  Protein disulfide-isomerase (P4HB) 6.6
 LDTP14427  Cytochrome c oxidase subunit 7A-related protein, mitochondrial (COX7A2L) 6.6
 LDTP18489  ADP-ribosylation factor-like protein 15 (ARL15) 6.5
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 6.5
 LDTP02006  Tissue alpha-L-fucosidase (FUCA1) 6.5
 LDTP07003  Presequence protease, mitochondrial (PITRM1) 6.4
 LDTP03257  Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) 6.3
 LDTP07591  Neutral cholesterol ester hydrolase 1 (NCEH1) 6.2
 LDTP09495  Probable glutathione peroxidase 8 (GPX8) 6.2
 LDTP00845  Probable 18S rRNA (guanine-N(7))-methyltransferase (BUD23) 6.0
 LDTP08617  Patatin-like phospholipase domain-containing protein 6 (PNPLA6) 6.0
 LDTP01699  Acyl-CoA 6-desaturase (FADS2) 5.9
 LDTP02074  Aldehyde dehydrogenase, mitochondrial (ALDH2) 5.8
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 5.8
 LDTP04289  Very long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADVL) 5.8
 LDTP01333  Isocitrate dehydrogenase [NADP] cytoplasmic (IDH1) 5.7
 LDTP10471  Protein disulfide-isomerase TMX3 (TMX3) 5.7
 LDTP04893  Ras-related protein Rab-5B (RAB5B) 5.7
 LDTP16258  N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (PIGL) 5.6
 LDTP04353  Protoporphyrinogen oxidase (PPOX) 5.6
 LDTP02860  Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) 5.6
 LDTP09835  GPI-anchor transamidase (PIGK) 5.5
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 5.5
 LDTP01168  NADH dehydrogenase iron-sulfur protein 2, mitochondrial (NDUFS2) 5.4
 LDTP04895  Ras-related protein Rab-10 (RAB10) 5.4
 LDTP10101  Peptidyl-prolyl cis-trans isomerase FKBP10 (FKBP10) 5.3
 LDTP04955  Ras-related protein R-Ras2 (RRAS2) 5.3
Transporter and channel
Click to Show/Hide to Show 39 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 22.3
 LDTP06198  Major facilitator superfamily domain-containing protein 10 (MFSD10) 22.2
 LDTP15948  Transmembrane protein 126A (TMEM126A) 18.1
 LDTP10985  Equilibrative nucleoside transporter 1 (SLC29A1) 13.7
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 12.6
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 9.3
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 9.2
 LDTP06308  Mitochondrial inner membrane protein OXA1L (OXA1L) 8.6
 LDTP07531  Sideroflexin-4 (SFXN4) 8.6
 LDTP00451  Secretory carrier-associated membrane protein 3 (SCAMP3) 8.5
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 7.9
 LDTP01060  PRA1 family protein 2 (PRAF2) 7.9
 LDTP07467  Rhomboid domain-containing protein 2 (RHBDD2) 7.8
 LDTP00477  Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) 7.1
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 7.1
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 6.8
 LDTP12191  Vezatin (VEZT) 6.8
 LDTP02544  Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1) 6.7
 LDTP01745  Putative lipid scramblase CLPTM1 (CLPTM1) 6.6
 LDTP07667  Transmembrane protein 205 (TMEM205) 6.6
 LDTP00865  Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) 6.1
 LDTP01200  Peroxisomal membrane protein PEX14 (PEX14) 6.1
 LDTP07058  Transmembrane protein 201 (TMEM201) 6.0
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 6.0
 LDTP01352  PRA1 family protein 3 (ARL6IP5) 5.9
 LDTP12958  ER membrane protein complex subunit 3 (EMC3) 5.9
 LDTP01234  Erlin-1 (ERLIN1) 5.9
 LDTP11229  Transmembrane protein 70, mitochondrial (TMEM70) 5.8
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 5.7
 LDTP07236  Protein GPR107 (GPR107) 5.6
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 5.6
 LDTP14235  Mitochondrial carrier homolog 2 (MTCH2) 5.6
 LDTP05511  Cytoskeleton-associated protein 4 (CKAP4) 5.5
 LDTP11095  Mitochondrial 2-oxodicarboxylate carrier (SLC25A21) 5.5
 LDTP03284  ATP synthase F(0) complex subunit B1, mitochondrial (ATP5PB) 5.5
 LDTP12909  Mitochondrial carrier homolog 1 (MTCH1) 5.4
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 5.4
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 5.3
 LDTP09333  Golgin subfamily A member 5 (GOLGA5) 5.3
Other
Click to Show/Hide to Show 41 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01075  Protein CutA (CUTA) 32.4
 LDTP05160  Nucleobindin-2 (NUCB2) 18.0
 LDTP08224  Protein LYRIC (MTDH) 11.2
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 10.5
 LDTP12369  Bromodomain-containing protein 7 (BRD7) 10.1
 LDTP01167  Reticulon-2 (RTN2) 9.6
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 8.7
 LDTP15623  Neuferricin (CYB5D2) 8.5
 LDTP11670  Mitochondrial fission factor (MFF) 8.5
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 8.2
 LDTP01522  Reticulon-3 (RTN3) 8.1
 LDTP04356  Emerin (EMD) 7.9
 LDTP12412  Reticulon-4 (RTN4) 7.8
 LDTP19963  TraB domain-containing protein (TRABD) 7.2
 LDTP01362  Survival of motor neuron-related-splicing factor 30 (SMNDC1) 7.1
 LDTP14458  Tetraspanin-6 (TSPAN6) 7.1
 LDTP01494  Protein YIF1A (YIF1A) 6.7
 LDTP15976  Large ribosomal subunit protein mL46 (MRPL46) 6.5
 LDTP11140  Peroxiredoxin-like 2A (PRXL2A) 6.5
 LDTP08300  Reticulophagy regulator 3 (RETREG3) 6.4
 LDTP16078  Heme-binding protein 1 (HEBP1) 6.3
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 6.1
 LDTP14939  Membralin (TMEM259) 6.1
 LDTP11143  Centromere protein K (CENPK) 6.0
 LDTP00105  Protein unc-119 homolog B (UNC119B) 6.0
 LDTP12971  Core histone macro-H2A.2 (MACROH2A2) 6.0
 LDTP18373  Thioredoxin-related transmembrane protein 4 (TMX4) 5.9
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 5.8
 LDTP05318  RNA-binding protein EWS (EWSR1) 5.7
 LDTP11981  Receptor expression-enhancing protein 4 (REEP4) 5.6
 LDTP07718  MICOS complex subunit MIC27 (APOOL) 5.5
 LDTP10056  Protein FAM162A (FAM162A) 5.5
 LDTP12755  OCIA domain-containing protein 1 (OCIAD1) 5.5
 LDTP13922  Tudor and KH domain-containing protein (TDRKH) 5.4
 LDTP13626  Ubiquilin-1 (UBQLN1) 5.4
 LDTP10927  COP9 signalosome complex subunit 8 (COPS8) 5.4
 LDTP04558  Arfaptin-1 (ARFIP1) 5.3
 LDTP12073  Golgi reassembly-stacking protein 2 (GORASP2) 5.3
 LDTP09069  Kinetochore protein Spc24 (SPC24) 5.3
 LDTP08904  Protein jagunal homolog 1 (JAGN1) 5.3
 LDTP07368  Tetratricopeptide repeat protein 19, mitochondrial (TTC19) 5.2

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587