Details of the Target

General Information of Target

Target ID LDTP00477
Target Name Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23)
Gene Name TIMM23
Gene ID 100287932
Synonyms
TIM23; Mitochondrial import inner membrane translocase subunit Tim23
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEGGGGSGNKTTGGLAGFFGAGGAGYSHADLAGVPLTGMNPLSPYLNVDPRYLVQDTDEF
ILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLKETQNMAWSKPRNVQILNMV
TRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNTVAAGTMTGMLYKCTGGLRGIARG
GLTGLTLTSLYALYNNWEHMKGSLLQQSL
Target Bioclass
Transporter and channel
Family
Tim17/Tim22/Tim23 family
Subcellular location
Mitochondrion inner membrane
Function Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
Uniprot ID
O14925
Ensemble ID
ENST00000580018.4
HGNC ID
HGNC:17312

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		14.04  LDD0402  [1]
YN-1
 Probe Info  		100.00  LDD0444  [2]
STPyne
 Probe Info  		K110(0.57)  LDD0277  [3]
Jackson_1
 Probe Info  		20.00  LDD0120  [4]
EA-probe
 Probe Info  		N.A.  LDD0440  [5]
IA-alkyne
 Probe Info  		C170(0.98)  LDD2206  [6]
DBIA
 Probe Info  		C170(0.96)  LDD1507  [7]
TFBX
 Probe Info  		N.A.  LDD0027  [8]
IPM
 Probe Info  		N.A.  LDD0005  [9]
AOyne
 Probe Info  		15.00  LDD0443  [10]
PAL-AfBPP Probe
Click To Hide/Show 103 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		6.41  LDD1714  [11]
C022
 Probe Info  		16.34  LDD1728  [11]
C027
 Probe Info  		7.11  LDD1733  [11]
C040
 Probe Info  		11.79  LDD1740  [11]
C041
 Probe Info  		5.70  LDD1741  [11]
C049
 Probe Info  		5.31  LDD1747  [11]
C055
 Probe Info  		15.78  LDD1752  [11]
C056
 Probe Info  		27.86  LDD1753  [11]
C063
 Probe Info  		15.35  LDD1760  [11]
C070
 Probe Info  		11.16  LDD1766  [11]
C072
 Probe Info  		7.52  LDD1768  [11]
C091
 Probe Info  		18.25  LDD1782  [11]
C092
 Probe Info  		39.40  LDD1783  [11]
C094
 Probe Info  		55.72  LDD1785  [11]
C095
 Probe Info  		4.92  LDD1786  [11]
C100
 Probe Info  		5.54  LDD1789  [11]
C106
 Probe Info  		50.56  LDD1793  [11]
C107
 Probe Info  		7.84  LDD1794  [11]
C108
 Probe Info  		20.39  LDD1795  [11]
C112
 Probe Info  		38.85  LDD1799  [11]
C134
 Probe Info  		38.32  LDD1816  [11]
C135
 Probe Info  		10.13  LDD1817  [11]
C139
 Probe Info  		10.85  LDD1821  [11]
C141
 Probe Info  		11.71  LDD1823  [11]
C143
 Probe Info  		19.97  LDD1825  [11]
C153
 Probe Info  		42.22  LDD1834  [11]
C159
 Probe Info  		9.58  LDD1839  [11]
C160
 Probe Info  		11.31  LDD1840  [11]
C161
 Probe Info  		18.13  LDD1841  [11]
C163
 Probe Info  		8.22  LDD1843  [11]
C169
 Probe Info  		30.70  LDD1849  [11]
C178
 Probe Info  		36.76  LDD1857  [11]
C186
 Probe Info  		8.34  LDD1864  [11]
C194
 Probe Info  		12.91  LDD1870  [11]
C198
 Probe Info  		10.13  LDD1874  [11]
C201
 Probe Info  		29.04  LDD1877  [11]
C210
 Probe Info  		74.03  LDD1884  [11]
C218
 Probe Info  		30.06  LDD1892  [11]
C219
 Probe Info  		5.39  LDD1893  [11]
C220
 Probe Info  		25.46  LDD1894  [11]
C225
 Probe Info  		6.19  LDD1898  [11]
C226
 Probe Info  		6.15  LDD1899  [11]
C228
 Probe Info  		38.05  LDD1901  [11]
C229
 Probe Info  		7.21  LDD1902  [11]
C231
 Probe Info  		33.13  LDD1904  [11]
C232
 Probe Info  		57.68  LDD1905  [11]
C233
 Probe Info  		12.55  LDD1906  [11]
C234
 Probe Info  		6.50  LDD1907  [11]
C235
 Probe Info  		30.70  LDD1908  [11]
C238
 Probe Info  		13.36  LDD1911  [11]
C240
 Probe Info  		14.32  LDD1913  [11]
C243
 Probe Info  		19.84  LDD1916  [11]
C244
 Probe Info  		14.32  LDD1917  [11]
C246
 Probe Info  		19.29  LDD1919  [11]
C252
 Probe Info  		9.51  LDD1925  [11]
C264
 Probe Info  		21.56  LDD1935  [11]
C265
 Probe Info  		16.11  LDD1936  [11]
C277
 Probe Info  		19.97  LDD1947  [11]
C278
 Probe Info  		50.91  LDD1948  [11]
C282
 Probe Info  		20.68  LDD1952  [11]
C285
 Probe Info  		57.68  LDD1955  [11]
C286
 Probe Info  		5.82  LDD1956  [11]
C287
 Probe Info  		16.68  LDD1957  [11]
C288
 Probe Info  		12.13  LDD1958  [11]
C289
 Probe Info  		84.45  LDD1959  [11]
C290
 Probe Info  		5.74  LDD1960  [11]
C293
 Probe Info  		42.52  LDD1963  [11]
C296
 Probe Info  		15.56  LDD1966  [11]
C299
 Probe Info  		6.87  LDD1968  [11]
C305
 Probe Info  		11.63  LDD1974  [11]
C310
 Probe Info  		14.42  LDD1977  [11]
C314
 Probe Info  		17.03  LDD1981  [11]
C339
 Probe Info  		6.02  LDD2002  [11]
C343
 Probe Info  		23.75  LDD2005  [11]
C348
 Probe Info  		11.79  LDD2009  [11]
C349
 Probe Info  		21.11  LDD2010  [11]
C350
 Probe Info  		61.39  LDD2011  [11]
C355
 Probe Info  		37.01  LDD2016  [11]
C356
 Probe Info  		9.58  LDD2017  [11]
C357
 Probe Info  		6.96  LDD2018  [11]
C362
 Probe Info  		77.71  LDD2023  [11]
C363
 Probe Info  		16.91  LDD2024  [11]
C364
 Probe Info  		18.77  LDD2025  [11]
C366
 Probe Info  		13.74  LDD2027  [11]
C367
 Probe Info  		6.50  LDD2028  [11]
C376
 Probe Info  		8.57  LDD2036  [11]
C382
 Probe Info  		9.58  LDD2041  [11]
C388
 Probe Info  		69.55  LDD2047  [11]
C390
 Probe Info  		84.45  LDD2049  [11]
C391
 Probe Info  		15.45  LDD2050  [11]
C399
 Probe Info  		7.31  LDD2058  [11]
C403
 Probe Info  		21.11  LDD2061  [11]
C407
 Probe Info  		14.52  LDD2064  [11]
C413
 Probe Info  		15.89  LDD2069  [11]
C429
 Probe Info  		16.34  LDD2084  [11]
C431
 Probe Info  		57.28  LDD2086  [11]
FFF probe11
 Probe Info  		20.00  LDD0471  [12]
FFF probe13
 Probe Info  		20.00  LDD0475  [12]
FFF probe14
 Probe Info  		20.00  LDD0477  [12]
FFF probe15
 Probe Info  		9.14  LDD0478  [12]
FFF probe2
 Probe Info  		20.00  LDD0463  [12]
FFF probe3
 Probe Info  		19.42  LDD0465  [12]
OEA-DA
 Probe Info  		20.00  LDD0046  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C170(0.96)  LDD1507  [7]
 LDCM0276  AC17 HEK-293T C170(1.05)  LDD1515  [7]
 LDCM0285  AC25 HEK-293T C170(1.14)  LDD1524  [7]
 LDCM0294  AC33 HEK-293T C170(0.99)  LDD1533  [7]
 LDCM0303  AC41 HEK-293T C170(1.01)  LDD1542  [7]
 LDCM0311  AC49 HEK-293T C170(1.02)  LDD1550  [7]
 LDCM0320  AC57 HEK-293T C170(1.02)  LDD1559  [7]
 LDCM0356  AKOS034007680 HEK-293T C170(1.05)  LDD1570  [7]
 LDCM0156  Aniline NCI-H1299 14.20  LDD0403  [1]
 LDCM0071  Cisar_cp14 MDA-MB-231 20.00  LDD0121  [4]
 LDCM0070  Cisar_cp37 MDA-MB-231 20.00  LDD0120  [4]
 LDCM0404  CL17 HEK-293T C170(1.14)  LDD1608  [7]
 LDCM0417  CL29 HEK-293T C170(1.05)  LDD1621  [7]
 LDCM0431  CL41 HEK-293T C170(1.02)  LDD1635  [7]
 LDCM0440  CL5 HEK-293T C170(1.10)  LDD1644  [7]
 LDCM0444  CL53 HEK-293T C170(1.13)  LDD1647  [7]
 LDCM0457  CL65 HEK-293T C170(1.10)  LDD1660  [7]
 LDCM0470  CL77 HEK-293T C170(1.04)  LDD1673  [7]
 LDCM0483  CL89 HEK-293T C170(1.01)  LDD1686  [7]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [5]
 LDCM0627  NUDT7-COV-1 HEK-293T C170(0.98)  LDD2206  [6]
 LDCM0628  OTUB2-COV-1 HEK-293T C170(0.82)  LDD2207  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP) EBP family Q15125
Vesicle transport protein GOT1A (GOLT1A) GOT1 family Q6ZVE7
Ribonuclease kappa (RNASEK) RNase K family Q6P5S7
17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13) Short-chain dehydrogenases/reductases (SDR) family Q7Z5P4
TLC domain-containing protein 4 (TLCD4) TLCD4 family Q96MV1
Lysoplasmalogenase TMEM86B (TMEM86B) TMEM86 family Q8N661
Transporter and channel
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Endophilin-B1 (SH3GLB1) Endophilin family Q9Y371
Monocarboxylate transporter 2 (SLC16A7) Monocarboxylate porter (TC 2.A.1.13) family O60669
Aquaporin-6 (AQP6) MIP/aquaporin (TC 1.A.8) family Q13520
Sodium channel regulatory subunit beta-3 (SCN3B) Sodium channel auxiliary subunit SCN3B family Q9NY72
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) Tim17/Tim22/Tim23 family O60830
Transmembrane protein 14B (TMEM14B) TMEM14 family Q9NUH8
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) BZIP family Q96BA8
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM210B, mitochondrial (FAM210B) FAM210 family Q96KR6
Protein jagunal homolog 1 (JAGN1) Jagunal family Q8N5M9
Tetraspanin-8 (TSPAN8) Tetraspanin (TM4SF) family P19075
Mitochondrial import inner membrane translocase subunit Tim21 (TIMM21) TIM21 family Q9BVV7
Transmembrane protein 88 (TMEM88) TMEM88 family Q6PEY1

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
5 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
6 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
9 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
10 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
11 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
12 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
13 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570