Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C100  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 61 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP00697  Cytochrome b5 type B (CYB5B) 27.3
 LDTP12959  NADH dehydrogenase 1 alpha subcomplex subunit 13 (NDUFA13) 18.5
 LDTP03540  Heme oxygenase 2 (HMOX2) 14.9
 LDTP02359  Heme oxygenase 1 (HMOX1) 14.0
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 13.5
 LDTP13189  Cytochrome b-c1 complex subunit 9 (UQCR10) 13.1
 LDTP08728  Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) 12.3
 LDTP15422  Prostaglandin reductase 3 (PTGR3) 12.0
 LDTP10182  TLC domain-containing protein 1 (TLCD1) 11.0
 LDTP05862  NAD(P) transhydrogenase, mitochondrial (NNT) 10.9
 LDTP09396  Minor histocompatibility antigen H13 (HM13) 10.9
 LDTP00486  Cytochrome b-c1 complex subunit 8 (UQCRQ) 10.7
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 10.2
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 10.1
 LDTP16007  Lipid droplet-associated hydrolase (LDAH) 9.9
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 9.1
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 8.8
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 8.4
 LDTP12253  Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM) 7.8
 LDTP06787  Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) 7.5
 LDTP18087  Dephospho-CoA kinase domain-containing protein (DCAKD) 7.4
 LDTP09061  Retinol dehydrogenase 13 (RDH13) 7.4
 LDTP05758  S-methyl-5'-thioadenosine phosphorylase (MTAP) 7.3
 LDTP01613  Sphingosine-1-phosphate lyase 1 (SGPL1) 7.1
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 7.0
 LDTP10020  Ras-related protein Rab-24 (RAB24) 6.9
 LDTP06142  Squalene monooxygenase (SQLE) 6.9
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 6.7
 LDTP03842  Squalene synthase (FDFT1) 6.7
 LDTP08385  NADH dehydrogenase 1 alpha subcomplex subunit 11 (NDUFA11) 6.6
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 6.5
 LDTP09251  Atlastin-2 (ATL2) 6.5
 LDTP13314  Enolase-phosphatase E1 (ENOPH1) 6.3
 LDTP06477  Methylsterol monooxygenase 1 (MSMO1) 6.2
 LDTP05409  Antigen peptide transporter 2 (TAP2) 6.1
 LDTP06325  3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP) 6.1
 LDTP01773  Cytochrome c oxidase subunit 2 (MT-CO2) 6.1
 LDTP07990  Heparan sulfate 2-O-sulfotransferase 1 (HS2ST1) 6.1
 LDTP07931  Heme A synthase COX15 (COX15) 5.9
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 5.8
 LDTP10329  Ceramide synthase 2 (CERS2) 5.8
 LDTP00428  Tripeptidyl-peptidase 1 (TPP1) 5.7
 LDTP02860  Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2) 5.6
 LDTP14030  V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) 5.6
 LDTP12954  Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3 (HACD3) 5.6
 LDTP01687  Apoptosis-inducing factor 1, mitochondrial (AIFM1) 5.5
 LDTP07756  Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 (HACD2) 5.5
 LDTP11127  ADP-dependent glucokinase (ADPGK) 5.5
 LDTP06901  Very-long-chain 3-oxoacyl-CoA reductase (HSD17B12) 5.5
 LDTP06617  Ceramide glucosyltransferase (UGCG) 5.4
 LDTP02000  3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) 5.4
 LDTP09542  1-acylglycerol-3-phosphate O-acyltransferase ABHD5 (ABHD5) 5.4
 LDTP09188  Lysophosphatidylcholine acyltransferase 1 (LPCAT1) 5.4
 LDTP11178  Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1) 5.3
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 5.3
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 5.2
 LDTP04120  Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A (STT3A) 5.2
 LDTP06371  GTP-binding protein Rheb (RHEB) 5.2
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 5.2
 LDTP11852  Presenilin-associated rhomboid-like protein, mitochondrial (PARL) 5.1
 LDTP05476  3-ketodihydrosphingosine reductase (KDSR) 5.1
Transporter and channel
Click to Show/Hide to Show 52 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 34.8
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 25.8
 LDTP15648  BRI3-binding protein (BRI3BP) 22.8
 LDTP08878  ER membrane protein complex subunit 5 (MMGT1) 21.6
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 19.8
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 19.0
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 17.6
 LDTP20002  Transmembrane protein 160 (TMEM160) 16.1
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 14.9
 LDTP14099  Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) 13.2
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 13.1
 LDTP10036  BOS complex subunit NCLN (NCLN) 12.0
 LDTP00405  Etoposide-induced protein 2.4 homolog (EI24) 11.3
 LDTP14235  Mitochondrial carrier homolog 2 (MTCH2) 11.1
 LDTP05511  Cytoskeleton-associated protein 4 (CKAP4) 10.1
 LDTP16813  Tetraspanin-3 (TSPAN3) 9.3
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 9.3
 LDTP08469  Complex I assembly factor TMEM126B, mitochondrial (TMEM126B) 9.3
 LDTP06365  Transmembrane emp24 domain-containing protein 2 (TMED2) 9.2
 LDTP00235  Membrane-associated progesterone receptor component 1 (PGRMC1) 8.9
 LDTP05900  Metaxin-1 (MTX1) 8.9
 LDTP07053  Cytochrome c oxidase assembly protein COX20, mitochondrial (COX20) 8.6
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 8.5
 LDTP10958  Sigma non-opioid intracellular receptor 1 (SIGMAR1) 8.5
 LDTP02215  Prosaposin (PSAP) 8.1
 LDTP06308  Mitochondrial inner membrane protein OXA1L (OXA1L) 8.0
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 7.9
 LDTP15948  Transmembrane protein 126A (TMEM126A) 7.8
 LDTP10499  Lipid scramblase CLPTM1L (CLPTM1L) 7.3
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 7.2
 LDTP18459  StAR-related lipid transfer protein 7, mitochondrial (STARD7) 7.2
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 7.1
 LDTP07583  Transmembrane protein 65 (TMEM65) 6.8
 LDTP11615  Protein tweety homolog 3 (TTYH3) 6.5
 LDTP01745  Putative lipid scramblase CLPTM1 (CLPTM1) 6.5
 LDTP02645  Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1) 6.2
 LDTP04459  H(+)/Cl(-) exchange transporter 3 (CLCN3) 6.1
 LDTP13969  Transmembrane emp24 domain-containing protein 7 (TMED7) 6.1
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 5.9
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 5.7
 LDTP10065  Transmembrane protein 230 (TMEM230) 5.7
 LDTP12510  Aladin (AAAS) 5.5
 LDTP00477  Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) 5.5
 LDTP12331  Endoplasmic reticulum membrane protein complex subunit 7 (EMC7) 5.5
 LDTP01525  Mitochondrial proton/calcium exchanger protein (LETM1) 5.5
 LDTP01200  Peroxisomal membrane protein PEX14 (PEX14) 5.4
 LDTP09515  Importin-4 (IPO4) 5.3
 LDTP06855  Anoctamin-6 (ANO6) 5.2
 LDTP09203  Nucleoporin Nup37 (NUP37) 5.2
 LDTP07058  Transmembrane protein 201 (TMEM201) 5.1
 LDTP13824  Voltage-dependent anion-selective channel protein 3 (VDAC3) 5.0
 LDTP11245  Derlin-1 (DERL1) 5.0
Transcription factor
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP10501  DnaJ homolog subfamily C member 1 (DNAJC1) 5.3
Other
Click to Show/Hide to Show 39 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP15420  Mitochondrial import receptor subunit TOM5 homolog (TOMM5) 28.4
 LDTP05160  Nucleobindin-2 (NUCB2) 16.4
 LDTP01522  Reticulon-3 (RTN3) 15.6
 LDTP13311  Poly(U)-binding-splicing factor PUF60 (PUF60) 14.8
 LDTP10056  Protein FAM162A (FAM162A) 13.9
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 13.8
 LDTP03678  ER lumen protein-retaining receptor 2 (KDELR2) 13.1
 LDTP11076  Apolipoprotein L2 (APOL2) 12.6
 LDTP12412  Reticulon-4 (RTN4) 12.2
 LDTP13086  Syntaxin-18 (STX18) 11.2
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 10.1
 LDTP15269  Large ribosomal subunit protein mL55 (MRPL55) 10.0
 LDTP15398  Protein FAM177A1 (FAM177A1) 9.3
 LDTP01494  Protein YIF1A (YIF1A) 9.2
 LDTP00859  Synaptogyrin-2 (SYNGR2) 9.1
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 9.1
 LDTP01167  Reticulon-2 (RTN2) 8.1
 LDTP12755  OCIA domain-containing protein 1 (OCIAD1) 7.9
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 7.6
 LDTP10908  Protein S100-A13 (S100A13) 7.5
 LDTP19963  TraB domain-containing protein (TRABD) 7.4
 LDTP05375  Nucleobindin-1 (NUCB1) 7.2
 LDTP10775  Endoplasmic reticulum-Golgi intermediate compartment protein 2 (ERGIC2) 6.8
 LDTP16080  Protein FAM114A2 (FAM114A2) 6.5
 LDTP16323  Signal recognition particle receptor subunit beta (SRPRB) 6.2
 LDTP11389  Bcl-2-like protein 13 (BCL2L13) 6.1
 LDTP11143  Centromere protein K (CENPK) 5.7
 LDTP19625  Integral membrane protein GPR180 (GPR180) 5.7
 LDTP14458  Tetraspanin-6 (TSPAN6) 5.7
 LDTP06097  Reticulocalbin-2 (RCN2) 5.7
 LDTP06291  ADP-ribosylation factor-like protein 6-interacting protein 1 (ARL6IP1) 5.6
 LDTP09324  Ganglioside-induced differentiation-associated protein 1 (GDAP1) 5.5
 LDTP04207  Heat shock 70 kDa protein 13 (HSPA13) 5.5
 LDTP08904  Protein jagunal homolog 1 (JAGN1) 5.4
 LDTP06128  RNA-binding protein 39 (RBM39) 5.3
 LDTP15240  LysM and putative peptidoglycan-binding domain-containing protein 3 (LYSMD3) 5.1
 LDTP10357  RUS family member 1 (RUSF1) 5.1
 LDTP07508  Polyamine-modulated factor 1 (PMF1) 5.0
 LDTP08154  Large ribosomal subunit protein uL10m (MRPL10) 5.0

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587