Details of the Target

General Information of Target

Target ID LDTP07756
Target Name Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 (HACD2)
Gene Name HACD2
Gene ID 201562
Synonyms
PTPLB; Very-long-chain; 3R)-3-hydroxyacyl-CoA dehydratase 2; EC 4.2.1.134; 3-hydroxyacyl-CoA dehydratase 2; HACD2; Protein-tyrosine phosphatase-like member B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAVAATAAAKGNGGGGGRAGAGDASGTRKKKGPGPLATAYLVIYNVVMTAGWLVIAVGL
VRAYLAKGSYHSLYYSIEKPLKFFQTGALLEILHCAIGIVPSSVVLTSFQVMSRVFLIWA
VTHSVKEVQSEDSVLLFVIAWTITEIIRYSFYTFSLLNHLPYLIKWARYTLFIVLYPMGV
SGELLTIYAALPFVRQAGLYSISLPNKYNFSFDYYAFLILIMISYIPIFPQLYFHMIHQR
RKILSHTEEHKKFE
Target Bioclass
Enzyme
Family
Very long-chain fatty acids dehydratase HACD family
Subcellular location
Endoplasmic reticulum membrane
Function
Catalyzes the third of the very long-chain fatty acids (VLCFA) elongation four-step cycle (condensation, reduction, dehydration, and reduction). This endoplasmic reticulum-elongation process is characterized by the addition of two carbons to the lipid chain through each cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of elongation. Therefore, it participates in the production of various VLCFAs involved in multiple biological processes as precursors of membrane lipids and lipid mediators.
Uniprot ID
Q6Y1H2
Ensemble ID
ENST00000383657.10
HGNC ID
HGNC:9640

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
STPyne
 Probe Info  		K242(1.08)  LDD0277  [2]
ATP probe
 Probe Info  		N.A.  LDD0199  [3]
NHS
 Probe Info  		N.A.  LDD0010  [4]
Acrolein
 Probe Info  		N.A.  LDD0217  [5]
Crotonaldehyde
 Probe Info  		H246(0.00); H250(0.00)  LDD0219  [5]
AOyne
 Probe Info  		15.00  LDD0443  [6]
PAL-AfBPP Probe
Click To Hide/Show 19 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C055
 Probe Info  		13.36  LDD1752  [7]
C056
 Probe Info  		22.16  LDD1753  [7]
C070
 Probe Info  		9.06  LDD1766  [7]
C092
 Probe Info  		18.25  LDD1783  [7]
C094
 Probe Info  		30.27  LDD1785  [7]
C095
 Probe Info  		5.46  LDD1786  [7]
C100
 Probe Info  		5.54  LDD1789  [7]
C112
 Probe Info  		22.01  LDD1799  [7]
C159
 Probe Info  		7.16  LDD1839  [7]
C161
 Probe Info  		13.55  LDD1841  [7]
C218
 Probe Info  		11.55  LDD1892  [7]
C220
 Probe Info  		13.55  LDD1894  [7]
C228
 Probe Info  		23.59  LDD1901  [7]
C289
 Probe Info  		27.10  LDD1959  [7]
C362
 Probe Info  		40.22  LDD2023  [7]
C366
 Probe Info  		5.70  LDD2027  [7]
C388
 Probe Info  		62.25  LDD2047  [7]
STS-1
 Probe Info  		N.A.  LDD0136  [8]
OEA-DA
 Probe Info  		9.06  LDD0046  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H250(0.00); H246(0.00)  LDD0222  [5]
 LDCM0107  IAA HeLa H246(0.00); H250(0.00)  LDD0221  [5]
 LDCM0109  NEM HeLa H246(0.00); H250(0.00)  LDD0223  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Very-long-chain enoyl-CoA reductase (TECR) Steroid 5-alpha reductase family Q9NZ01
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
B-cell receptor-associated protein 31 (BCAP31) BCAP29/BCAP31 family P51572
Small integral membrane protein 1 (SMIM1) SMIM1 family B2RUZ4
Thioredoxin-related transmembrane protein 2 (TMX2) . Q9Y320
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) BZIP family Q96BA8
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Interleukin-10 receptor subunit alpha (IL10RA) Type II cytokine receptor family Q13651
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein FAM209A (FAM209A) FAM209 family Q5JX71

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
4 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
9 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570