Details of the Target

General Information of Target

Target ID LDTP05375
Target Name Nucleobindin-1 (NUCB1)
Gene Name NUCB1
Gene ID 4924
Synonyms
NUC; Nucleobindin-1; CALNUC
3D Structure
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2D Sequence (FASTA)
Download
3D Structure (PDB)
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Sequence
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVID
VLETDGHFREKLQAANAEDIKSGKLSRELDFVSHHVRTKLDELKRQEVSRLRMLLKAKMD
AEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYE
MLKEHERRRYLESLGEEQRKEAERKLEEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDP
NRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHV
MKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAE
LNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQL
KFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL
Target Type
Literature-reported
Target Bioclass
Other
Family
Nucleobindin family
Subcellular location
Golgi apparatus, cis-Golgi network membrane
Function
Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis. Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins).
TTD ID
T98134
Uniprot ID
Q02818
DrugMap ID
TT6A5ZL
Ensemble ID
ENST00000405315.9
HGNC ID
HGNC:8043

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		13.01  LDD0318  [1]
C-Sul
 Probe Info  		3.01  LDD0066  [2]
ONAyne
 Probe Info  		K177(9.64)  LDD0274  [3]
STPyne
 Probe Info  		K118(10.00); K177(10.00); K364(2.09); K400(7.69)  LDD0277  [3]
OPA-S-S-alkyne
 Probe Info  		K177(1.71); K302(2.22); K364(2.75); K183(3.16)  LDD3494  [4]
Probe 1
 Probe Info  		Y179(55.31)  LDD3495  [5]
5E-2FA
 Probe Info  		H131(0.00); H415(0.00); H172(0.00); H395(0.00)  LDD2235  [6]
ATP probe
 Probe Info  		K421(0.00); K71(0.00); K218(0.00)  LDD0199  [7]
m-APA
 Probe Info  		H131(0.00); H415(0.00); H172(0.00); H395(0.00)  LDD2231  [6]
Acrolein
 Probe Info  		H460(0.00); H415(0.00); H423(0.00); H67(0.00)  LDD0217  [8]
PAL-AfBPP Probe
Click To Hide/Show 156 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		5.86  LDD1714  [9]
C007
 Probe Info  		8.28  LDD1716  [9]
C010
 Probe Info  		6.92  LDD1719  [9]
C011
 Probe Info  		5.58  LDD1720  [9]
C017
 Probe Info  		8.40  LDD1725  [9]
C018
 Probe Info  		8.51  LDD1726  [9]
C022
 Probe Info  		30.06  LDD1728  [9]
C039
 Probe Info  		8.63  LDD1739  [9]
C040
 Probe Info  		43.71  LDD1740  [9]
C041
 Probe Info  		33.59  LDD1741  [9]
C044
 Probe Info  		6.45  LDD1743  [9]
C049
 Probe Info  		9.71  LDD1747  [9]
C053
 Probe Info  		10.56  LDD1751  [9]
C055
 Probe Info  		49.87  LDD1752  [9]
C056
 Probe Info  		50.91  LDD1753  [9]
C059
 Probe Info  		15.14  LDD1756  [9]
C060
 Probe Info  		9.06  LDD1757  [9]
C063
 Probe Info  		65.80  LDD1760  [9]
C064
 Probe Info  		15.56  LDD1761  [9]
C065
 Probe Info  		10.06  LDD1762  [9]
C070
 Probe Info  		9.19  LDD1766  [9]
C072
 Probe Info  		14.03  LDD1768  [9]
C082
 Probe Info  		10.13  LDD1774  [9]
C085
 Probe Info  		9.92  LDD1777  [9]
C087
 Probe Info  		11.63  LDD1779  [9]
C089
 Probe Info  		4.92  LDD1781  [9]
C091
 Probe Info  		24.93  LDD1782  [9]
C092
 Probe Info  		40.50  LDD1783  [9]
C094
 Probe Info  		65.34  LDD1785  [9]
C095
 Probe Info  		9.19  LDD1786  [9]
C099
 Probe Info  		6.36  LDD1788  [9]
C100
 Probe Info  		7.16  LDD1789  [9]
C106
 Probe Info  		21.71  LDD1793  [9]
C107
 Probe Info  		7.89  LDD1794  [9]
C108
 Probe Info  		9.06  LDD1795  [9]
C112
 Probe Info  		37.27  LDD1799  [9]
C122
 Probe Info  		5.90  LDD1808  [9]
C129
 Probe Info  		8.11  LDD1811  [9]
C130
 Probe Info  		13.55  LDD1812  [9]
C134
 Probe Info  		64.45  LDD1816  [9]
C135
 Probe Info  		29.86  LDD1817  [9]
C138
 Probe Info  		4.99  LDD1820  [9]
C139
 Probe Info  		22.94  LDD1821  [9]
C140
 Probe Info  		15.89  LDD1822  [9]
C141
 Probe Info  		21.11  LDD1823  [9]
C143
 Probe Info  		28.05  LDD1825  [9]
C147
 Probe Info  		12.13  LDD1829  [9]
C153
 Probe Info  		43.11  LDD1834  [9]
C156
 Probe Info  		5.78  LDD1836  [9]
C158
 Probe Info  		12.55  LDD1838  [9]
C159
 Probe Info  		25.63  LDD1839  [9]
C160
 Probe Info  		41.36  LDD1840  [9]
C161
 Probe Info  		54.95  LDD1841  [9]
C163
 Probe Info  		12.21  LDD1843  [9]
C165
 Probe Info  		13.45  LDD1845  [9]
C166
 Probe Info  		7.21  LDD1846  [9]
C169
 Probe Info  		54.19  LDD1849  [9]
C178
 Probe Info  		25.28  LDD1857  [9]
C183
 Probe Info  		7.21  LDD1861  [9]
C185
 Probe Info  		6.15  LDD1863  [9]
C187
 Probe Info  		19.29  LDD1865  [9]
C191
 Probe Info  		21.11  LDD1868  [9]
C193
 Probe Info  		9.51  LDD1869  [9]
C194
 Probe Info  		18.51  LDD1870  [9]
C196
 Probe Info  		19.43  LDD1872  [9]
C197
 Probe Info  		6.50  LDD1873  [9]
C201
 Probe Info  		70.03  LDD1877  [9]
C204
 Probe Info  		5.66  LDD1879  [9]
C205
 Probe Info  		5.17  LDD1880  [9]
C206
 Probe Info  		40.50  LDD1881  [9]
C211
 Probe Info  		15.89  LDD1885  [9]
C213
 Probe Info  		22.47  LDD1887  [9]
C214
 Probe Info  		7.01  LDD1888  [9]
C218
 Probe Info  		19.70  LDD1892  [9]
C219
 Probe Info  		8.88  LDD1893  [9]
C220
 Probe Info  		25.99  LDD1894  [9]
C222
 Probe Info  		5.78  LDD1896  [9]
C225
 Probe Info  		13.18  LDD1898  [9]
C226
 Probe Info  		16.56  LDD1899  [9]
C228
 Probe Info  		49.18  LDD1901  [9]
C238
 Probe Info  		13.55  LDD1911  [9]
C240
 Probe Info  		11.96  LDD1913  [9]
C243
 Probe Info  		26.54  LDD1916  [9]
C244
 Probe Info  		21.71  LDD1917  [9]
C246
 Probe Info  		17.27  LDD1919  [9]
C252
 Probe Info  		13.18  LDD1925  [9]
C264
 Probe Info  		20.11  LDD1935  [9]
C265
 Probe Info  		20.53  LDD1936  [9]
C277
 Probe Info  		11.55  LDD1947  [9]
C282
 Probe Info  		35.02  LDD1952  [9]
C284
 Probe Info  		25.99  LDD1954  [9]
C285
 Probe Info  		34.30  LDD1955  [9]
C287
 Probe Info  		10.34  LDD1957  [9]
C288
 Probe Info  		10.56  LDD1958  [9]
C289
 Probe Info  		25.46  LDD1959  [9]
C290
 Probe Info  		8.00  LDD1960  [9]
C293
 Probe Info  		19.43  LDD1963  [9]
C296
 Probe Info  		12.04  LDD1966  [9]
C299
 Probe Info  		11.71  LDD1968  [9]
C305
 Probe Info  		18.38  LDD1974  [9]
C310
 Probe Info  		8.88  LDD1977  [9]
C326
 Probe Info  		7.52  LDD1990  [9]
C333
 Probe Info  		6.06  LDD1996  [9]
C338
 Probe Info  		23.43  LDD2001  [9]
C339
 Probe Info  		7.21  LDD2002  [9]
C342
 Probe Info  		5.28  LDD2004  [9]
C343
 Probe Info  		38.05  LDD2005  [9]
C346
 Probe Info  		14.03  LDD2007  [9]
C347
 Probe Info  		6.02  LDD2008  [9]
C349
 Probe Info  		16.91  LDD2010  [9]
C350
 Probe Info  		44.02  LDD2011  [9]
C353
 Probe Info  		7.78  LDD2014  [9]
C354
 Probe Info  		8.40  LDD2015  [9]
C355
 Probe Info  		37.27  LDD2016  [9]
C356
 Probe Info  		24.08  LDD2017  [9]
C357
 Probe Info  		10.93  LDD2018  [9]
C361
 Probe Info  		20.82  LDD2022  [9]
C362
 Probe Info  		27.28  LDD2023  [9]
C363
 Probe Info  		18.51  LDD2024  [9]
C366
 Probe Info  		18.77  LDD2027  [9]
C367
 Probe Info  		6.96  LDD2028  [9]
C376
 Probe Info  		10.85  LDD2036  [9]
C383
 Probe Info  		20.53  LDD2042  [9]
C386
 Probe Info  		13.45  LDD2045  [9]
C387
 Probe Info  		6.77  LDD2046  [9]
C388
 Probe Info  		65.80  LDD2047  [9]
C390
 Probe Info  		99.73  LDD2049  [9]
C391
 Probe Info  		33.59  LDD2050  [9]
C392
 Probe Info  		5.28  LDD2051  [9]
C393
 Probe Info  		6.73  LDD2052  [9]
C396
 Probe Info  		7.67  LDD2055  [9]
C403
 Probe Info  		14.22  LDD2061  [9]
C405
 Probe Info  		11.96  LDD2063  [9]
C407
 Probe Info  		32.67  LDD2064  [9]
C408
 Probe Info  		9.92  LDD2065  [9]
C409
 Probe Info  		5.70  LDD2066  [9]
C419
 Probe Info  		12.47  LDD2074  [9]
C420
 Probe Info  		10.20  LDD2075  [9]
C429
 Probe Info  		99.73  LDD2084  [9]
C430
 Probe Info  		5.17  LDD2085  [9]
C431
 Probe Info  		71.51  LDD2086  [9]
C433
 Probe Info  		5.62  LDD2088  [9]
FFF probe11
 Probe Info  		20.00  LDD0471  [10]
FFF probe12
 Probe Info  		16.52  LDD0473  [10]
FFF probe13
 Probe Info  		20.00  LDD0475  [10]
FFF probe14
 Probe Info  		20.00  LDD0477  [10]
FFF probe15
 Probe Info  		20.00  LDD0478  [10]
FFF probe2
 Probe Info  		20.00  LDD0463  [10]
FFF probe3
 Probe Info  		20.00  LDD0464  [10]
FFF probe4
 Probe Info  		20.00  LDD0466  [10]
FFF probe6
 Probe Info  		20.00  LDD0467  [10]
FFF probe7
 Probe Info  		20.00  LDD0483  [10]
FFF probe9
 Probe Info  		20.00  LDD0470  [10]
JN0003
 Probe Info  		20.00  LDD0469  [10]
TM-F
 Probe Info  		T64(0.00); D65(0.00)  LDD0020  [11]
OEA-DA
 Probe Info  		20.00  LDD0046  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H415(0.00); H460(0.00); H423(0.00)  LDD0222  [8]
 LDCM0185  Compound 17 HEK-293T 3.98  LDD0512  [10]
 LDCM0191  Compound 21 HEK-293T 9.95  LDD0508  [10]
 LDCM0190  Compound 34 HEK-293T 9.76  LDD0510  [10]
 LDCM0192  Compound 35 HEK-293T 9.43  LDD0509  [10]
 LDCM0193  Compound 36 HEK-293T 10.05  LDD0511  [10]
 LDCM0107  IAA HeLa H460(0.00); H423(0.00)  LDD0221  [8]
 LDCM0109  NEM HeLa H172(0.00); H67(0.00); H460(0.00); H415(0.00)  LDD0223  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067

The Drug(s) Related To This Target

Approved
Click To Hide/Show 3 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Calcium Phosphate Small molecular drug DB11348
Calcium Phosphate Dihydrate Small molecular drug DB14481
Calcium Citrate . DB11093
Investigative
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Kml110 Small molecular drug D0S9AT
Mjn228 Small molecular drug D01XLZ

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
5 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
10 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
11 Evaluation of fully-functionalized diazirine tags for chemical proteomic applications. Chem Sci. 2021 May 7;12(22):7839-7847. doi: 10.1039/d1sc01360b.
Mass spectrometry data entry: PXD025652
12 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570