Details of the Probe

General Information of Probe

Probe ID
LDPC0477
Probe Name
C244
Synonyms
Z4286591554
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 370.4
Lipid-water partition coefficient (xlogp) 1.5
Rotatable Bond Count (rotbonds) 8
Hydrogen Bond Donor Count (hbonddonor) 2
Hydrogen Bond Acceptor Count (hbondacc) 7
Chemical Identifiers
Formula
C18H19FN6O2
IUPAC Name
5-N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]-2-(4-fluorophenyl)-3,4-dihydropyrazole-3,5-dicarboxamide
Canonical SMILES
C#CCCC1(N=N1)CCNC(=O)C2=NN(C(C2)C(=O)N)C3=CC=C(C=C3)F
InChI
InChI=1S/C18H19FN6O2/c1-2-3-8-18(23-24-18)9-10-21-17(27)14-11-15(16(20)26)25(22-14)13-6-4-12(19)5-7-13/h1,4-7,15H,3,8-11H2,(H2,20,26)(H,21,27)
InChIKey
XSQZNOAEQNKJEV-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

64 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
3-ketodihydrosphingosine reductase (KDSR) LDTP05476 27.10  LDD1917 
7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) LDTP10032 24.08  LDD1917 
Acetyl-CoA acetyltransferase, cytosolic (ACAT2) LDTP11321 17.88  LDD1917 
Adenosine deaminase (ADA) LDTP01803 39.67  LDD1917 
Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) LDTP04437 18.64  LDD1917 
Antigen peptide transporter 1 (TAP1) LDTP05408 16.91  LDD1917 
Apoptosis-inducing factor 1, mitochondrial (AIFM1) LDTP01687 38.32  LDD1917 
CAAX prenyl protease 1 homolog (ZMPSTE24) LDTP01328 15.24  LDD1917 
Carbohydrate sulfotransferase 14 (CHST14) LDTP09096 18.51  LDD1917 
Carboxymethylenebutenolidase homolog (CMBL) LDTP10215 25.81  LDD1917 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 23.43  LDD1917 
Cartilage-associated protein (CRTAP) LDTP01300 28.44  LDD1917 
CDGSH iron-sulfur domain-containing protein 1 (CISD1) LDTP12873 24.93  LDD1917 
Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) LDTP03189 30.27  LDD1917 
Cytochrome b-c1 complex subunit 8 (UQCRQ) LDTP00486 16.91  LDD1917 
Cytochrome b-c1 complex subunit 9 (UQCR10) LDTP13189 25.99  LDD1917 
Cytochrome b5 type B (CYB5B) LDTP00697 26.17  LDD1917 
Dehydrogenase/reductase SDR family member 1 (DHRS1) LDTP10550 25.28  LDD1917 
Dolichyldiphosphatase 1 (DOLPP1) LDTP15328 21.26  LDD1917 
Dynamin-2 (DNM2) LDTP04374 16.45  LDD1917 
E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) LDTP10699 15.67  LDD1917 
Endoplasmic reticulum aminopeptidase 1 (ERAP1) LDTP12868 20.53  LDD1917 
Endoplasmic reticulum protein SC65 (P3H4) LDTP09874 17.88  LDD1917 
Endoplasmic reticulum transmembrane helix translocase (ATP13A1) LDTP12279 15.56  LDD1917 
Ferrochelatase, mitochondrial (FECH) LDTP03198 27.67  LDD1917 
Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM) LDTP12253 22.63  LDD1917 
GTP:AMP phosphotransferase AK3, mitochondrial (AK3) LDTP13352 25.63  LDD1917 
Heme oxygenase 1 (HMOX1) LDTP02359 59.71  LDD1917 
Heme oxygenase 2 (HMOX2) LDTP03540 39.67  LDD1917 
Holocytochrome c-type synthase (HCCS) LDTP04581 14.62  LDD1917 
Isoleucine--tRNA ligase, mitochondrial (IARS2) LDTP12588 14.93  LDD1917 
L-2-hydroxyglutarate dehydrogenase, mitochondrial (L2HGDH) LDTP12105 18.51  LDD1917 
Leukotriene A-4 hydrolase (LTA4H) LDTP02385 19.16  LDD1917 
Lipid droplet-associated hydrolase (LDAH) LDTP16007 39.12  LDD1917 
Lon protease homolog, mitochondrial (LONP1) LDTP03817 13.93  LDD1917 
Lysophosphatidic acid phosphatase type 6 (ACP6) LDTP12363 30.70  LDD1917 
Mitochondrial cardiolipin hydrolase (PLD6) LDTP08790 15.67  LDD1917 
N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) LDTP11273 15.24  LDD1917 
NAD-dependent protein deacetylase sirtuin-2 (SIRT2) LDTP08598 25.46  LDD1917 
NADH dehydrogenase 1 alpha subcomplex subunit 10, mitochondrial (NDUFA10) LDTP01561 66.26  LDD1917 
NADH dehydrogenase 1 alpha subcomplex subunit 13 (NDUFA13) LDTP12959 50.56  LDD1917 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 41.64  LDD1917 
NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) LDTP01982 17.51  LDD1917 
Obg-like ATPase 1 (OLA1) LDTP12610 16.45  LDD1917 
Peptidyl-prolyl cis-trans isomerase FKBP14 (FKBP14) LDTP12720 18.25  LDD1917 
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) LDTP14218 21.86  LDD1917 
Peroxisomal trans-2-enoyl-CoA reductase (PECR) LDTP11437 20.53  LDD1917 
Phospholipase A2 group XV (PLA2G15) LDTP09088 29.24  LDD1917 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 36.25  LDD1917 
Prenylcysteine oxidase-like (PCYOX1L) LDTP15480 18.64  LDD1917 
Presequence protease, mitochondrial (PITRM1) LDTP07003 13.83  LDD1917 
Prolyl 3-hydroxylase 1 (P3H1) LDTP06750 16.91  LDD1917 
Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) LDTP02679 20.53  LDD1917 
Prostaglandin reductase 3 (PTGR3) LDTP15422 32.22  LDD1917 
Proteasome subunit alpha type-2 (PSMA2) LDTP03331 30.06  LDD1917 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 17.15  LDD1917 
Proteasome subunit beta type-4 (PSMB4) LDTP03417 26.54  LDD1917 
Protein O-mannosyl-transferase TMTC3 (TMTC3) LDTP07868 16.91  LDD1917 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 49.18  LDD1917 
Puromycin-sensitive aminopeptidase (NPEPPS) LDTP04712 20.82  LDD1917 
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) LDTP00907 14.72  LDD1917 
Transmembrane protein with metallophosphoesterase domain (TMPPE) LDTP07831 23.59  LDD1917 
Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCHL3) LDTP02783 24.25  LDD1917 
Ubiquitin-protein ligase E3A (UBE3A) LDTP05446 16.68  LDD1917 
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⏷ Show the Full List of 64 Enzyme
51 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
45 kDa calcium-binding protein (SDF4) LDTP11116 20.53  LDD1917 
ATP synthase subunit a (MT-ATP6) LDTP01804 33.59  LDD1917 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 38.85  LDD1917 
BRI3-binding protein (BRI3BP) LDTP15648 15.45  LDD1917 
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1) LDTP02645 13.93  LDD1917 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 45.25  LDD1917 
Cytoskeleton-associated protein 4 (CKAP4) LDTP05511 22.16  LDD1917 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 32.00  LDD1917 
Golgi SNAP receptor complex member 1 (GOSR1) LDTP01542 17.51  LDD1917 
Golgin subfamily A member 5 (GOLGA5) LDTP09333 15.45  LDD1917 
High affinity cationic amino acid transporter 1 (SLC7A1) LDTP03559 16.22  LDD1917 
Importin-9 (IPO9) LDTP10663 30.48  LDD1917 
Integral membrane protein 2B (ITM2B) LDTP13833 14.22  LDD1917 
Intraflagellar transport protein 56 (IFT56) LDTP00010 15.89  LDD1917 
Lysosome membrane protein 2 (SCARB2) LDTP06034 14.03  LDD1917 
Melanoma inhibitory activity protein 2 (MIA2) LDTP10669 14.32  LDD1917 
Metaxin-1 (MTX1) LDTP05900 22.16  LDD1917 
Mitochondrial 2-oxodicarboxylate carrier (SLC25A21) LDTP11095 42.52  LDD1917 
Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) LDTP00865 17.63  LDD1917 
Mitochondrial dicarboxylate carrier (SLC25A10) LDTP13174 24.59  LDD1917 
Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) LDTP13983 19.56  LDD1917 
Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) LDTP10913 57.28  LDD1917 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 26.72  LDD1917 
Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) LDTP00477 14.32  LDD1917 
Mitochondrial inner membrane protein OXA1L (OXA1L) LDTP06308 19.29  LDD1917 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 31.12  LDD1917 
Mpv17-like protein 2 (MPV17L2) LDTP14952 16.11  LDD1917 
Nucleoporin Nup37 (NUP37) LDTP09203 16.34  LDD1917 
Peroxisomal biogenesis factor 19 (PEX19) LDTP03905 14.42  LDD1917 
Peroxisomal membrane protein 2 (PXMP2) LDTP16069 18.38  LDD1917 
Protein ERGIC-53 (LMAN1) LDTP04237 13.93  LDD1917 
Protein FAM8A1 (FAM8A1) LDTP16190 14.32  LDD1917 
Protein GPR107 (GPR107) LDTP07236 31.78  LDD1917 
Protein sel-1 homolog 1 (SEL1L) LDTP13164 14.52  LDD1917 
Protein wntless homolog (WLS) LDTP07156 39.67  LDD1917 
Sigma intracellular receptor 2 (TMEM97) LDTP06945 15.45  LDD1917 
Sigma non-opioid intracellular receptor 1 (SIGMAR1) LDTP10958 29.65  LDD1917 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 23.59  LDD1917 
Solute carrier family 25 member 33 (SLC25A33) LDTP11162 18.64  LDD1917 
Syntaxin-4 (STX4) LDTP05667 15.56  LDD1917 
Translocating chain-associated membrane protein 1 (TRAM1) LDTP06426 36.25  LDD1917 
Translocator protein (TSPO) LDTP03546 30.48  LDD1917 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 58.08  LDD1917 
Transmembrane 9 superfamily member 3 (TM9SF3) LDTP18448 16.00  LDD1917 
Transmembrane protein 126A (TMEM126A) LDTP15948 37.27  LDD1917 
Transmembrane protein 160 (TMEM160) LDTP20002 22.47  LDD1917 
Transmembrane protein 199 (TMEM199) LDTP08881 27.86  LDD1917 
V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) LDTP09968 24.59  LDD1917 
V-type proton ATPase subunit E 1 (ATP6V1E1) LDTP03811 17.75  LDD1917 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 36.25  LDD1917 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 28.84  LDD1917 
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⏷ Show the Full List of 51 Transporter and channel
75 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
26S proteasome non-ATPase regulatory subunit 11 (PSMD11) LDTP00223 16.11  LDD1917 
Anaphase-promoting complex subunit 16 (ANAPC16) LDTP10213 16.45  LDD1917 
Apolipoprotein L2 (APOL2) LDTP11076 99.73  LDD1917 
Calumenin (CALU) LDTP00887 16.00  LDD1917 
Caprin-1 (CAPRIN1) LDTP06121 14.62  LDD1917 
Centrosomal protein 43 (CEP43) LDTP01652 14.62  LDD1917 
Charged multivesicular body protein 7 (CHMP7) LDTP09589 14.72  LDD1917 
Clustered mitochondria protein homolog (CLUH) LDTP14486 22.78  LDD1917 
COP9 signalosome complex subunit 8 (COPS8) LDTP10927 14.93  LDD1917 
CYFIP-related Rac1 interactor B (CYRIB) LDTP12642 14.32  LDD1917 
Diablo IAP-binding mitochondrial protein (DIABLO) LDTP12468 18.00  LDD1917 
Dynactin subunit 3 (DCTN3) LDTP01359 23.75  LDD1917 
Endoplasmic reticulum-Golgi intermediate compartment protein 2 (ERGIC2) LDTP10775 17.15  LDD1917 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 21.86  LDD1917 
ER lumen protein-retaining receptor 2 (KDELR2) LDTP03678 31.56  LDD1917 
ER lumen protein-retaining receptor 3 (KDELR3) LDTP00850 21.71  LDD1917 
Eukaryotic translation initiation factor 5 (EIF5) LDTP04652 24.76  LDD1917 
Golgi integral membrane protein 4 (GOLIM4) LDTP00281 27.28  LDD1917 
Golgi phosphoprotein 3 (GOLPH3) LDTP11911 18.00  LDD1917 
HAUS augmin-like complex subunit 4 (HAUS4) LDTP11978 15.78  LDD1917 
HAUS augmin-like complex subunit 5 (HAUS5) LDTP01466 14.83  LDD1917 
HCLS1-associated protein X-1 (HAX1) LDTP00199 20.39  LDD1917 
Heme-binding protein 1 (HEBP1) LDTP16078 27.47  LDD1917 
Inhibitor of nuclear factor kappa-B kinase-interacting protein (IKBIP) LDTP07891 21.11  LDD1917 
Keratin, type I cytoskeletal 18 (KRT18) LDTP02124 22.94  LDD1917 
Kinetochore protein Spc24 (SPC24) LDTP09069 15.24  LDD1917 
Laminin subunit gamma-1 (LAMC1) LDTP02535 14.83  LDD1917 
Large proline-rich protein BAG6 (BAG6) LDTP04094 17.27  LDD1917 
LysM and putative peptidoglycan-binding domain-containing protein 3 (LYSMD3) LDTP15240 15.03  LDD1917 
Matrix-remodeling-associated protein 7 (MXRA7) LDTP17003 14.72  LDD1917 
Membralin (TMEM259) LDTP14939 36.76  LDD1917 
Mitochondrial fission process protein 1 (MTFP1) LDTP16192 36.76  LDD1917 
Mitochondrial import inner membrane translocase subunit Tim9 (TIMM9) LDTP14126 17.75  LDD1917 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 31.34  LDD1917 
Mitochondrial import receptor subunit TOM5 homolog (TOMM5) LDTP15420 17.03  LDD1917 
Negative elongation factor C/D (NELFCD) LDTP08594 45.25  LDD1917 
Nestin (NES) LDTP04206 14.32  LDD1917 
Neuferricin (CYB5D2) LDTP15623 16.11  LDD1917 
Neurofilament light polypeptide (NEFL) LDTP02180 19.29  LDD1917 
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (NFU1) LDTP13622 14.32  LDD1917 
Nucleobindin-1 (NUCB1) LDTP05375 21.71  LDD1917 
Nucleobindin-2 (NUCB2) LDTP05160 43.41  LDD1917 
Optic atrophy 3 protein (OPA3) LDTP16006 25.11  LDD1917 
p53 and DNA damage-regulated protein 1 (PDRG1) LDTP16099 16.45  LDD1917 
Poly(U)-binding-splicing factor PUF60 (PUF60) LDTP13311 23.43  LDD1917 
Protein bicaudal D homolog 2 (BICD2) LDTP09410 13.83  LDD1917 
Protein CEBPZOS (CEBPZOS) LDTP18883 17.51  LDD1917 
Protein CutA (CUTA) LDTP01075 16.11  LDD1917 
Protein FAM114A2 (FAM114A2) LDTP16080 18.13  LDD1917 
Protein FAM162A (FAM162A) LDTP10056 24.42  LDD1917 
Protein jagunal homolog 1 (JAGN1) LDTP08904 15.45  LDD1917 
Protein NipSnap homolog 1 (NIPSNAP1) LDTP19912 16.91  LDD1917 
Protein SET (SET) LDTP05277 17.75  LDD1917 
Protein SYS1 homolog (SYS1) LDTP08796 19.97  LDD1917 
Rab3 GTPase-activating protein catalytic subunit (RAB3GAP1) LDTP06292 18.25  LDD1917 
Ran guanine nucleotide release factor (RANGRF) LDTP12287 20.53  LDD1917 
Receptor expression-enhancing protein 2 (REEP2) LDTP11113 15.78  LDD1917 
Reticulocalbin-2 (RCN2) LDTP06097 24.76  LDD1917 
Reticulon-4 (RTN4) LDTP12412 14.32  LDD1917 
RNA-binding protein FUS (FUS) LDTP03775 15.14  LDD1917 
Signal transducing adapter molecule 1 (STAM) LDTP09870 22.32  LDD1917 
Small ribosomal subunit protein eS10 (RPS10) LDTP04111 14.22  LDD1917 
Small ribosomal subunit protein uS9m (MRPS9) LDTP14792 30.48  LDD1917 
Synaptogyrin-2 (SYNGR2) LDTP00859 16.91  LDD1917 
Syntaxin-10 (STX10) LDTP00986 29.24  LDD1917 
Syntaxin-18 (STX18) LDTP13086 16.68  LDD1917 
Tetratricopeptide repeat protein 19, mitochondrial (TTC19) LDTP07368 14.62  LDD1917 
Tetratricopeptide repeat protein 38 (TTC38) LDTP19372 16.45  LDD1917 
Thioredoxin-related transmembrane protein 4 (TMX4) LDTP18373 18.90  LDD1917 
TraB domain-containing protein (TRABD) LDTP19963 18.00  LDD1917 
Transmembrane protein 35B (TMEM35B) LDTP19785 23.92  LDD1917 
U4/U6 small nuclear ribonucleoprotein Prp31 (PRPF31) LDTP09671 14.22  LDD1917 
UBX domain-containing protein 1 (UBXN1) LDTP05418 13.83  LDD1917 
UPF0488 protein C8orf33 (C8orf33) LDTP12025 23.26  LDD1917 
Vimentin (VIM) LDTP02297 20.11  LDD1917 
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⏷ Show the Full List of 75 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD1917 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587