Details of the Target

General Information of Target

Target ID LDTP02645
Target Name Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1)
Gene Name COX4I1
Gene ID 1327
Synonyms
COX4; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQK
ALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEWKTVVGGAMFFIGFTALVIMW
QKHYVYGPLPQSFDKEWVAKQTKRMLDMKVNPIQGLASKWDYEKNEWKK
Target Bioclass
Transporter and channel
Family
Cytochrome c oxidase IV family
Subcellular location
Mitochondrion inner membrane
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Uniprot ID
P13073
Ensemble ID
ENST00000253452.8
HGNC ID
HGNC:2265

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 18 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
HDSF-alk
 Probe Info  		2.18  LDD0197  [1]
FBPP2
 Probe Info  		2.62  LDD0318  [2]
TH211
 Probe Info  		Y38(9.15)  LDD0260  [3]
C-Sul
 Probe Info  		4.23  LDD0066  [4]
ONAyne
 Probe Info  		K87(0.00); K53(0.00)  LDD0273  [5]
Probe 1
 Probe Info  		Y126(6.99)  LDD3495  [6]
HPAP
 Probe Info  		3.13  LDD0063  [7]
5E-2FA
 Probe Info  		N.A.  LDD2235  [8]
ATP probe
 Probe Info  		K60(0.00); K87(0.00); K29(0.00); K149(0.00)  LDD0199  [9]
m-APA
 Probe Info  		H51(0.00); H44(0.00)  LDD2231  [8]
NHS
 Probe Info  		K60(0.00); K29(0.00); K87(0.00)  LDD0010  [10]
SF
 Probe Info  		N.A.  LDD0028  [11]
STPyne
 Probe Info  		K149(0.00); K159(0.00)  LDD0009  [10]
1c-yne
 Probe Info  		N.A.  LDD0228  [12]
Acrolein
 Probe Info  		H44(0.00); H51(0.00); H24(0.00)  LDD0217  [13]
Crotonaldehyde
 Probe Info  		H54(0.00); H44(0.00); H24(0.00)  LDD0219  [13]
Methacrolein
 Probe Info  		H44(0.00); H54(0.00); H24(0.00)  LDD0218  [13]
AOyne
 Probe Info  		15.00  LDD0443  [14]
PAL-AfBPP Probe
Click To Hide/Show 93 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C010
 Probe Info  		5.90  LDD1719  [15]
C022
 Probe Info  		15.24  LDD1728  [15]
C040
 Probe Info  		28.44  LDD1740  [15]
C041
 Probe Info  		8.00  LDD1741  [15]
C049
 Probe Info  		5.82  LDD1747  [15]
C053
 Probe Info  		6.73  LDD1751  [15]
C055
 Probe Info  		19.97  LDD1752  [15]
C056
 Probe Info  		45.57  LDD1753  [15]
C063
 Probe Info  		16.56  LDD1760  [15]
C070
 Probe Info  		9.85  LDD1766  [15]
C072
 Probe Info  		7.94  LDD1768  [15]
C082
 Probe Info  		5.17  LDD1774  [15]
C091
 Probe Info  		25.81  LDD1782  [15]
C092
 Probe Info  		42.81  LDD1783  [15]
C094
 Probe Info  		68.59  LDD1785  [15]
C095
 Probe Info  		6.87  LDD1786  [15]
C100
 Probe Info  		6.19  LDD1789  [15]
C106
 Probe Info  		39.67  LDD1793  [15]
C107
 Probe Info  		7.52  LDD1794  [15]
C108
 Probe Info  		9.13  LDD1795  [15]
C112
 Probe Info  		44.94  LDD1799  [15]
C134
 Probe Info  		41.07  LDD1816  [15]
C141
 Probe Info  		10.78  LDD1823  [15]
C143
 Probe Info  		25.28  LDD1825  [15]
C153
 Probe Info  		17.39  LDD1834  [15]
C159
 Probe Info  		8.34  LDD1839  [15]
C160
 Probe Info  		7.01  LDD1840  [15]
C161
 Probe Info  		17.15  LDD1841  [15]
C163
 Probe Info  		5.94  LDD1843  [15]
C165
 Probe Info  		13.45  LDD1845  [15]
C169
 Probe Info  		23.43  LDD1849  [15]
C178
 Probe Info  		19.16  LDD1857  [15]
C187
 Probe Info  		63.12  LDD1865  [15]
C191
 Probe Info  		19.03  LDD1868  [15]
C193
 Probe Info  		7.31  LDD1869  [15]
C196
 Probe Info  		13.00  LDD1872  [15]
C198
 Probe Info  		10.70  LDD1874  [15]
C201
 Probe Info  		70.52  LDD1877  [15]
C206
 Probe Info  		18.77  LDD1881  [15]
C210
 Probe Info  		60.13  LDD1884  [15]
C218
 Probe Info  		22.63  LDD1892  [15]
C220
 Probe Info  		25.81  LDD1894  [15]
C228
 Probe Info  		23.26  LDD1901  [15]
C231
 Probe Info  		32.45  LDD1904  [15]
C232
 Probe Info  		52.35  LDD1905  [15]
C233
 Probe Info  		8.94  LDD1906  [15]
C235
 Probe Info  		39.12  LDD1908  [15]
C238
 Probe Info  		17.88  LDD1911  [15]
C243
 Probe Info  		12.91  LDD1916  [15]
C244
 Probe Info  		13.93  LDD1917  [15]
C246
 Probe Info  		24.59  LDD1919  [15]
C264
 Probe Info  		18.25  LDD1935  [15]
C277
 Probe Info  		7.52  LDD1947  [15]
C278
 Probe Info  		54.95  LDD1948  [15]
C285
 Probe Info  		28.25  LDD1955  [15]
C287
 Probe Info  		15.89  LDD1957  [15]
C288
 Probe Info  		7.73  LDD1958  [15]
C289
 Probe Info  		91.77  LDD1959  [15]
C293
 Probe Info  		23.92  LDD1963  [15]
C310
 Probe Info  		7.89  LDD1977  [15]
C313
 Probe Info  		12.55  LDD1980  [15]
C314
 Probe Info  		14.52  LDD1981  [15]
C322
 Probe Info  		6.68  LDD1988  [15]
C326
 Probe Info  		8.22  LDD1990  [15]
C339
 Probe Info  		7.57  LDD2002  [15]
C343
 Probe Info  		14.62  LDD2005  [15]
C348
 Probe Info  		13.55  LDD2009  [15]
C349
 Probe Info  		17.39  LDD2010  [15]
C350
 Probe Info  		62.68  LDD2011  [15]
C355
 Probe Info  		31.34  LDD2016  [15]
C356
 Probe Info  		10.13  LDD2017  [15]
C362
 Probe Info  		80.45  LDD2023  [15]
C363
 Probe Info  		16.91  LDD2024  [15]
C364
 Probe Info  		19.84  LDD2025  [15]
C366
 Probe Info  		5.86  LDD2027  [15]
C382
 Probe Info  		9.45  LDD2041  [15]
C388
 Probe Info  		99.73  LDD2047  [15]
C390
 Probe Info  		99.73  LDD2049  [15]
C391
 Probe Info  		18.90  LDD2050  [15]
C392
 Probe Info  		5.98  LDD2051  [15]
C393
 Probe Info  		5.35  LDD2052  [15]
C396
 Probe Info  		5.24  LDD2055  [15]
C399
 Probe Info  		8.06  LDD2058  [15]
C403
 Probe Info  		26.72  LDD2061  [15]
C407
 Probe Info  		19.16  LDD2064  [15]
C426
 Probe Info  		13.45  LDD2081  [15]
FFF probe11
 Probe Info  		18.84  LDD0471  [16]
FFF probe13
 Probe Info  		20.00  LDD0475  [16]
FFF probe14
 Probe Info  		20.00  LDD0477  [16]
FFF probe2
 Probe Info  		20.00  LDD0463  [16]
FFF probe3
 Probe Info  		18.30  LDD0464  [16]
FFF probe4
 Probe Info  		11.68  LDD0466  [16]
OEA-DA
 Probe Info  		12.17  LDD0046  [17]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H51(0.00); H44(0.00); H24(0.00); H54(0.00)  LDD0222  [13]
 LDCM0107  IAA HeLa H44(0.00); H51(0.00); H24(0.00); H54(0.00)  LDD0221  [13]
 LDCM0109  NEM HeLa H44(0.00); H51(0.00); H24(0.00); H54(0.00)  LDD0223  [13]
 LDCM0014  Panhematin K562 3.13  LDD0063  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytochrome c oxidase subunit 1 (MT-CO1) Heme-copper respiratory oxidase family P00395
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Syntenin-1 (SDCBP) . O00560
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Krueppel-like factor 11 (KLF11) Sp1 C2H2-type zinc-finger protein family O14901

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Cholic Acid Small molecular drug DB02659
Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
N-formylmethionine Small molecular drug DB04464

References

1 Fatty Acyl Sulfonyl Fluoride as an Activity-Based Probe for Profiling Fatty Acid-Associated Proteins in Living Cells. Chembiochem. 2022 Feb 16;23(4):e202100628. doi: 10.1002/cbic.202100628. Epub 2021 Dec 30.
2 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
7 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
8 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
9 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
10 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
11 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
12 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
13 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
14 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
15 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
16 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
17 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570