Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C399  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 94 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP04051  Nicotinamide phosphoribosyltransferase (NAMPT) 66.7
 LDTP12013  dCTP pyrophosphatase 1 (DCTPP1) 26.9
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 25.5
 LDTP02359  Heme oxygenase 1 (HMOX1) 24.3
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 23.8
 LDTP00697  Cytochrome b5 type B (CYB5B) 22.5
 LDTP06333  Serum paraoxonase/arylesterase 2 (PON2) 21.4
 LDTP11081  NAD-capped RNA hydrolase NUDT12 (NUDT12) 21.3
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 21.3
 LDTP01982  NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) 21.0
 LDTP00428  Tripeptidyl-peptidase 1 (TPP1) 20.5
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 20.1
 LDTP05758  S-methyl-5'-thioadenosine phosphorylase (MTAP) 18.9
 LDTP03540  Heme oxygenase 2 (HMOX2) 18.8
 LDTP04333  GMP synthase [glutamine-hydrolyzing] (GMPS) 18.4
 LDTP10182  TLC domain-containing protein 1 (TLCD1) 17.9
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 17.1
 LDTP12873  CDGSH iron-sulfur domain-containing protein 1 (CISD1) 17.0
 LDTP16007  Lipid droplet-associated hydrolase (LDAH) 17.0
 LDTP03787  Choline kinase alpha (CHKA) 16.9
 LDTP00988  Protein O-GlcNAcase (OGA) 16.9
 LDTP01803  Adenosine deaminase (ADA) 16.4
 LDTP16032  Retinoid-inducible serine carboxypeptidase (SCPEP1) 15.0
 LDTP06787  Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) 14.6
 LDTP06617  Ceramide glucosyltransferase (UGCG) 14.0
 LDTP13620  Lysosomal thioesterase PPT2 (PPT2) 13.9
 LDTP02505  Lysosomal protective protein (CTSA) 13.6
 LDTP10020  Ras-related protein Rab-24 (RAB24) 13.6
 LDTP13189  Cytochrome b-c1 complex subunit 9 (UQCR10) 13.1
 LDTP15988  Probable serine carboxypeptidase CPVL (CPVL) 13.1
 LDTP11321  Acetyl-CoA acetyltransferase, cytosolic (ACAT2) 12.8
 LDTP10188  FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) 12.8
 LDTP08476  5'-3' exonuclease PLD3 (PLD3) 12.6
 LDTP10032  7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) 12.6
 LDTP07259  N-alpha-acetyltransferase 35, NatC auxiliary subunit (NAA35) 12.5
 LDTP09088  Phospholipase A2 group XV (PLA2G15) 12.5
 LDTP00486  Cytochrome b-c1 complex subunit 8 (UQCRQ) 12.3
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 12.2
 LDTP11127  ADP-dependent glucokinase (ADPGK) 12.0
 LDTP02005  Lysosomal acid glucosylceramidase (GBA1) 12.0
 LDTP02837  Beta-galactosidase (GLB1) 11.8
 LDTP05128  Glutathione S-transferase omega-1 (GSTO1) 11.8
 LDTP07990  Heparan sulfate 2-O-sulfotransferase 1 (HS2ST1) 11.6
 LDTP05476  3-ketodihydrosphingosine reductase (KDSR) 11.5
 LDTP17305  Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) 11.4
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 11.4
 LDTP04004  Lysosomal Pro-X carboxypeptidase (PRCP) 11.3
 LDTP09256  Putative phospholipase B-like 2 (PLBD2) 10.7
 LDTP05905  Acid ceramidase (ASAH1) 10.6
 LDTP01769  Dihydrofolate reductase (DHFR) 10.6
 LDTP06477  Methylsterol monooxygenase 1 (MSMO1) 9.9
 LDTP12253  Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM) 9.8
 LDTP04008  Endothelin-converting enzyme 1 (ECE1) 9.8
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 9.7
 LDTP04634  Alpha-N-acetylglucosaminidase (NAGLU) 9.6
 LDTP03257  Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) 9.6
 LDTP00544  Sphingolipid delta(4)-desaturase DES1 (DEGS1) 9.5
 LDTP12868  Endoplasmic reticulum aminopeptidase 1 (ERAP1) 9.3
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 9.3
 LDTP03198  Ferrochelatase, mitochondrial (FECH) 9.3
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 9.0
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 9.0
 LDTP01241  Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) 8.9
 LDTP16040  Glyoxalase domain-containing protein 4 (GLOD4) 8.9
 LDTP03417  Proteasome subunit beta type-4 (PSMB4) 8.9
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 8.9
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 8.8
 LDTP03854  Vitamin K-dependent gamma-carboxylase (GGCX) 8.7
 LDTP10550  Dehydrogenase/reductase SDR family member 1 (DHRS1) 8.6
 LDTP15328  Dolichyldiphosphatase 1 (DOLPP1) 8.6
 LDTP12363  Lysophosphatidic acid phosphatase type 6 (ACP6) 8.6
 LDTP10329  Ceramide synthase 2 (CERS2) 7.9
 LDTP15484  Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) 7.9
 LDTP08728  Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) 7.9
 LDTP11703  Histone deacetylase complex subunit SAP130 (SAP130) 7.9
 LDTP12628  Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) 7.9
 LDTP01759  Histone-lysine N-methyltransferase NSD2 (NSD2) 7.8
 LDTP17410  Cytochrome P450 20A1 (CYP20A1) 7.7
 LDTP03418  Proteasome subunit beta type-6 (PSMB6) 7.7
 LDTP04689  Adenosine kinase (ADK) 7.6
 LDTP10888  Legumain (LGMN) 7.6
 LDTP02486  Lysosomal alpha-glucosidase (GAA) 7.6
 LDTP08385  NADH dehydrogenase 1 alpha subcomplex subunit 11 (NDUFA11) 7.5
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 7.3
 LDTP10930  Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (PKMYT1) 7.3
 LDTP04114  E3 ubiquitin-protein ligase NEDD4 (NEDD4) 7.3
 LDTP05862  NAD(P) transhydrogenase, mitochondrial (NNT) 7.2
 LDTP02539  Lysosomal acid phosphatase (ACP2) 7.2
 LDTP03523  Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform (PPP2R1B) 7.1
 LDTP09251  Atlastin-2 (ATL2) 6.8
 LDTP11107  Serine/threonine-protein phosphatase CPPED1 (CPPED1) 6.8
 LDTP02254  ATP-dependent translocase ABCB1 (ABCB1) 6.7
 LDTP11606  Ethanolaminephosphotransferase 1 (SELENOI) 6.7
 LDTP11273  N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) 6.7
Transporter and channel
Click to Show/Hide to Show 67 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 99.7
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 49.9
 LDTP06365  Transmembrane emp24 domain-containing protein 2 (TMED2) 33.4
 LDTP01804  ATP synthase subunit a (MT-ATP6) 29.2
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 28.6
 LDTP15948  Transmembrane protein 126A (TMEM126A) 26.2
 LDTP15481  Transmembrane protein 87A (TMEM87A) 25.6
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 25.6
 LDTP12383  Protein GPR108 (GPR108) 25.3
 LDTP15648  BRI3-binding protein (BRI3BP) 21.9
 LDTP08878  ER membrane protein complex subunit 5 (MMGT1) 21.9
 LDTP11162  Solute carrier family 25 member 33 (SLC25A33) 19.8
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 18.9
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 18.8
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 18.3
 LDTP00592  Surfeit locus protein 4 (SURF4) 17.8
 LDTP08881  Transmembrane protein 199 (TMEM199) 17.8
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 17.4
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 16.1
 LDTP11812  Protein ARV1 (ARV1) 15.7
 LDTP03559  High affinity cationic amino acid transporter 1 (SLC7A1) 14.9
 LDTP13969  Transmembrane emp24 domain-containing protein 7 (TMED7) 14.8
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 14.6
 LDTP06221  StAR-related lipid transfer protein 3 (STARD3) 14.6
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 14.3
 LDTP11615  Protein tweety homolog 3 (TTYH3) 14.2
 LDTP07236  Protein GPR107 (GPR107) 13.9
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 13.3
 LDTP17362  Magnesium transporter NIPA3 (NIPAL1) 13.1
 LDTP05900  Metaxin-1 (MTX1) 12.9
 LDTP06945  Sigma intracellular receptor 2 (TMEM97) 12.8
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 12.6
 LDTP13142  Vacuolar protein sorting-associated protein 29 (VPS29) 12.0
 LDTP10958  Sigma non-opioid intracellular receptor 1 (SIGMAR1) 11.6
 LDTP16813  Tetraspanin-3 (TSPAN3) 11.2
 LDTP20002  Transmembrane protein 160 (TMEM160) 11.2
 LDTP12451  Integral membrane protein 2C (ITM2C) 11.1
 LDTP09328  Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) 10.7
 LDTP12207  Putative divalent cation/proton antiporter TMEM165 (TMEM165) 10.5
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 10.3
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 9.6
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 9.2
 LDTP01745  Putative lipid scramblase CLPTM1 (CLPTM1) 9.1
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 8.9
 LDTP11844  Protein spinster homolog 1 (SPNS1) 8.9
 LDTP02071  Amyloid-beta precursor protein (APP) 8.8
 LDTP10343  Vacuole membrane protein 1 (VMP1) 8.8
 LDTP10769  Intermembrane lipid transfer protein VPS13A (VPS13A) 8.8
 LDTP16069  Peroxisomal membrane protein 2 (PXMP2) 8.6
 LDTP02608  ADP/ATP translocase 1 (SLC25A4) 8.5
 LDTP00541  NPC intracellular cholesterol transporter 1 (NPC1) 8.3
 LDTP02645  Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (COX4I1) 8.1
 LDTP03380  Stomatin (STOM) 8.0
 LDTP11213  Nucleoporin NDC1 (NDC1) 7.9
 LDTP10669  Melanoma inhibitory activity protein 2 (MIA2) 7.7
 LDTP08279  Mitochondrial coenzyme A transporter SLC25A42 (SLC25A42) 7.7
 LDTP05978  THO complex subunit 5 homolog (THOC5) 7.7
 LDTP09968  V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) 7.5
 LDTP00477  Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) 7.3
 LDTP15845  Transmembrane 9 superfamily member 2 (TM9SF2) 7.2
 LDTP04592  Monocarboxylate transporter 1 (SLC16A1) 7.1
 LDTP01542  Golgi SNAP receptor complex member 1 (GOSR1) 7.1
 LDTP00857  Syntaxin-6 (STX6) 7.1
 LDTP09288  Vang-like protein 1 (VANGL1) 7.0
 LDTP17641  Solute carrier family 35 member F2 (SLC35F2) 6.9
 LDTP16163  Cytochrome c oxidase assembly protein COX16 homolog, mitochondrial (COX16) 6.8
 LDTP07439  Mitochondrial adenyl nucleotide antiporter SLC25A25 (SLC25A25) 6.8
Transcription factor
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP10501  DnaJ homolog subfamily C member 1 (DNAJC1) 7.8
Other
Click to Show/Hide to Show 62 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 65.3
 LDTP05160  Nucleobindin-2 (NUCB2) 37.3
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 31.3
 LDTP14458  Tetraspanin-6 (TSPAN6) 23.8
 LDTP18883  Protein CEBPZOS (CEBPZOS) 22.0
 LDTP08716  Abnormal spindle-like microcephaly-associated protein (ASPM) 20.1
 LDTP13086  Syntaxin-18 (STX18) 19.7
 LDTP01522  Reticulon-3 (RTN3) 19.2
 LDTP00986  Syntaxin-10 (STX10) 17.6
 LDTP13606  Mammalian ependymin-related protein 1 (EPDR1) 17.4
 LDTP16080  Protein FAM114A2 (FAM114A2) 16.1
 LDTP00105  Protein unc-119 homolog B (UNC119B) 15.7
 LDTP15420  Mitochondrial import receptor subunit TOM5 homolog (TOMM5) 15.0
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 14.4
 LDTP14080  U6 snRNA-associated Sm-like protein LSm5 (LSM5) 13.9
 LDTP14939  Membralin (TMEM259) 13.5
 LDTP10056  Protein FAM162A (FAM162A) 13.5
 LDTP15599  UPF0729 protein C18orf32 (C18orf32) 13.0
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 12.6
 LDTP18373  Thioredoxin-related transmembrane protein 4 (TMX4) 12.4
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 11.9
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 11.6
 LDTP05868  Protein unc-119 homolog A (UNC119) 11.1
 LDTP12412  Reticulon-4 (RTN4) 10.9
 LDTP01652  Centrosomal protein 43 (CEP43) 10.7
 LDTP00859  Synaptogyrin-2 (SYNGR2) 10.1
 LDTP10191  Gamma-tubulin complex component 3 (TUBGCP3) 10.0
 LDTP06305  WD repeat-containing protein 43 (WDR43) 9.4
 LDTP09773  Protein FAM3C (FAM3C) 9.4
 LDTP04304  Translation initiation factor eIF2B subunit beta (EIF2B2) 9.3
 LDTP19963  TraB domain-containing protein (TRABD) 9.2
 LDTP01075  Protein CutA (CUTA) 9.1
 LDTP13311  Poly(U)-binding-splicing factor PUF60 (PUF60) 9.0
 LDTP04094  Large proline-rich protein BAG6 (BAG6) 8.9
 LDTP16006  Optic atrophy 3 protein (OPA3) 8.8
 LDTP10849  Regulator of microtubule dynamics protein 3 (RMDN3) 8.6
 LDTP13125  Protein UXT (UXT) 8.5
 LDTP19372  Tetratricopeptide repeat protein 38 (TTC38) 8.5
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 8.3
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 8.2
 LDTP16078  Heme-binding protein 1 (HEBP1) 8.1
 LDTP04652  Eukaryotic translation initiation factor 5 (EIF5) 8.1
 LDTP08689  Dyslexia-associated protein KIAA0319-like protein (KIAA0319L) 8.0
 LDTP03926  Centrin-2 (CETN2) 7.9
 LDTP16323  Signal recognition particle receptor subunit beta (SRPRB) 7.8
 LDTP14263  TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L (TAF6L) 7.8
 LDTP11766  Activating signal cointegrator 1 complex subunit 2 (ASCC2) 7.7
 LDTP12468  Diablo IAP-binding mitochondrial protein (DIABLO) 7.6
 LDTP15253  HEAT repeat-containing protein 3 (HEATR3) 7.5
 LDTP06097  Reticulocalbin-2 (RCN2) 7.4
 LDTP15398  Protein FAM177A1 (FAM177A1) 7.2
 LDTP08904  Protein jagunal homolog 1 (JAGN1) 7.0
 LDTP01494  Protein YIF1A (YIF1A) 7.0
 LDTP12788  BRISC and BRCA1-A complex member 2 (BABAM2) 6.9
 LDTP06373  Mitochondrial import receptor subunit TOM20 homolog (TOMM20) 6.9
 LDTP12891  Glycolipid transfer protein (GLTP) 6.8
 LDTP11911  Golgi phosphoprotein 3 (GOLPH3) 6.8
 LDTP06250  Proteasome activator complex subunit 4 (PSME4) 6.8
 LDTP13782  RNA transcription, translation and transport factor protein (RTRAF) 6.8
 LDTP09143  Divergent protein kinase domain 2A (DIPK2A) 6.7
 LDTP11328  Splicing factor 3B subunit 5 (SF3B5) 6.7
 LDTP18132  Protein FAM136A (FAM136A) 6.7

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587