Details of the Target

General Information of Target

Target ID LDTP16078
Target Name Heme-binding protein 1 (HEBP1)
Gene Name HEBP1
Gene ID 50865
Synonyms
HBP; Heme-binding protein 1; p22HBP
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAAAAAAAAVGVRLRDCCSRGAVLLLFFSLSPRPPAAAAWLLGLRPEDTAGGRVSLEGG
TLRAAEGTSFLLRVYFQPGPPATAAPVPSPTLNSGENGTGDWAPRLVFIEEPPGGGGVAP
SAVPTRPPGPQRCREQSDWASDVEVLGPLRPGGVAGSALVQVRVRELRKGEAERGGAGGG
GKLFSLCAWDGRAWHHHGAAGGFLLRVRPRLYGPGGDLLPPAWLRALGALLLLALSALFS
GLRLSLLSLDPVELRVLRNSGSAAEQEQARRVQAVRGRGTHLLCTLLLGQAGANAALAGW
LYTSLPPGFGGTGEDYSEEGIHFPWLPALVCTGAVFLGAEICPYSVCSRHGLAIASHSVC
LTRLLMAAAFPVCYPLGRLLDWALRQEISTFYTREKLLETLRAADPYSDLVKEELNIIQG
ALELRTKVVEEVLTPLGDCFMLRSDAVLDFATVSEILRSGYTRIPVYEGDQRHNIVDILF
VKDLAFVDPDDCTPLLTVTRFYNRPLHCVFNDTRLDTVLEEFKKGKSHLAIVQRVNNEGE
GDPFYEVMGIVTLEDIIEEIIKSEILDETDLYTDNRKKQRVPQRERKRHDFSLFKLSDTE
MRVKISPQLLLATHRFMATEVEPFKSLYLSEKILLRLLKHPNVIQELKFDEKNKKAPEHY
LYQRNRPVDYFVLLLQGKVEVEVGKEGLRFENGAFTYYGVPAIMTTACSDNDVRKVGSLA
GSSVFLNRSPSRCSGLNRSESPNRERSDFGGSNTQLYSSSNNLYMPDYSVHILSDVQFVK
ITRQQYQNALTACHMDSSPQSPDMEAFTDGDSTKAPTTRGTPQTPKDDPAITLLNNRNSL
PCSRSDGLRSPSEVVYLRMEELAFTQEEMTDFEEHSTQQLTLSPAAVPTRAASDSECCNI
NLDTETSPCSSDFEENVGKKLLRTLSGQKRKRSPEGERTSEDNSNLTPLIT
Target Bioclass
Other
Family
HEBP family
Subcellular location
Cytoplasm
Function
May bind free porphyrinogens that may be present in the cell and thus facilitate removal of these potentially toxic compound. Binds with a high affinity to one molecule of heme or porphyrins. It binds metalloporphyrins, free porphyrins and N-methylprotoporphyrin with similar affinities.
Uniprot ID
Q9NRV9
Ensemble ID
ENST00000014930.9
HGNC ID
HGNC:17176

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 17 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		6.90  LDD0066  [1]
TH211
 Probe Info  		Y65(10.72)  LDD0257  [2]
TH216
 Probe Info  		Y138(11.87)  LDD0259  [2]
STPyne
 Probe Info  		K118(8.33); K64(2.54)  LDD0277  [3]
ONAyne
 Probe Info  		K121(10.00)  LDD0275  [3]
Probe 1
 Probe Info  		Y31(18.38); Y144(12.42)  LDD3495  [4]
DBIA
 Probe Info  		C36(1.88)  LDD3314  [5]
P13
 Probe Info  		13.94  LDD0203  [6]
Jackson_1
 Probe Info  		2.14  LDD0120  [7]
HPAP
 Probe Info  		3.35  LDD0062  [8]
HHS-482
 Probe Info  		Y138(0.83); Y178(0.68)  LDD0285  [9]
HHS-475
 Probe Info  		Y138(0.45)  LDD0264  [10]
HHS-465
 Probe Info  		Y178(3.54)  LDD2237  [11]
1d-yne
 Probe Info  		N.A.  LDD0358  [12]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [13]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [14]
NAIA_5
 Probe Info  		C36(0.00); C168(0.00)  LDD2223  [15]
PAL-AfBPP Probe
Click To Hide/Show 25 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C027
 Probe Info  		6.28  LDD1733  [16]
C056
 Probe Info  		18.38  LDD1753  [16]
C094
 Probe Info  		39.95  LDD1785  [16]
C145
 Probe Info  		8.28  LDD1827  [16]
C158
 Probe Info  		12.30  LDD1838  [16]
C206
 Probe Info  		15.67  LDD1881  [16]
C234
 Probe Info  		6.02  LDD1907  [16]
C235
 Probe Info  		99.73  LDD1908  [16]
C244
 Probe Info  		27.47  LDD1917  [16]
C249
 Probe Info  		14.62  LDD1922  [16]
C251
 Probe Info  		27.86  LDD1924  [16]
C252
 Probe Info  		9.78  LDD1925  [16]
C266
 Probe Info  		7.94  LDD1937  [16]
C285
 Probe Info  		22.01  LDD1955  [16]
C287
 Probe Info  		15.35  LDD1957  [16]
C289
 Probe Info  		55.33  LDD1959  [16]
C299
 Probe Info  		81.01  LDD1968  [16]
C346
 Probe Info  		34.30  LDD2007  [16]
C347
 Probe Info  		11.55  LDD2008  [16]
C355
 Probe Info  		29.24  LDD2016  [16]
C361
 Probe Info  		39.40  LDD2022  [16]
C373
 Probe Info  		44.94  LDD2033  [16]
C399
 Probe Info  		8.11  LDD2058  [16]
C403
 Probe Info  		16.34  LDD2061  [16]
DA-2
 Probe Info  		N.A.  LDD0072  [17]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0088  C45 HEK-293T 13.94  LDD0203  [6]
 LDCM0070  Cisar_cp37 MDA-MB-231 2.14  LDD0120  [7]
 LDCM0116  HHS-0101 DM93 Y138(0.45)  LDD0264  [10]
 LDCM0117  HHS-0201 DM93 Y138(1.13)  LDD0265  [10]
 LDCM0118  HHS-0301 DM93 Y138(1.07)  LDD0266  [10]
 LDCM0119  HHS-0401 DM93 Y138(1.58)  LDD0267  [10]
 LDCM0120  HHS-0701 DM93 Y138(0.76)  LDD0268  [10]
 LDCM0123  JWB131 DM93 Y138(0.83); Y178(0.68)  LDD0285  [9]
 LDCM0124  JWB142 DM93 Y138(1.03); Y178(0.41)  LDD0286  [9]
 LDCM0125  JWB146 DM93 Y138(1.16); Y178(0.82)  LDD0287  [9]
 LDCM0126  JWB150 DM93 Y138(2.26); Y178(5.99)  LDD0288  [9]
 LDCM0127  JWB152 DM93 Y138(4.45); Y178(0.19)  LDD0289  [9]
 LDCM0128  JWB198 DM93 Y138(0.15); Y178(1.77)  LDD0290  [9]
 LDCM0129  JWB202 DM93 Y138(0.71); Y178(0.38)  LDD0291  [9]
 LDCM0130  JWB211 DM93 Y138(1.18); Y178(1.05)  LDD0292  [9]
 LDCM0022  KB02 769-P C36(1.81)  LDD2246  [5]
 LDCM0023  KB03 769-P C36(2.01)  LDD2663  [5]
 LDCM0024  KB05 IGR37 C36(1.88)  LDD3314  [5]
 LDCM0014  Panhematin HEK-293T 3.35  LDD0062  [8]
 LDCM0131  RA190 MM1.R C168(1.55)  LDD0304  [18]

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Discovery of Potent and Selective Inhibitors against Protein-Derived Electrophilic Cofactors. J Am Chem Soc. 2022 Mar 30;144(12):5377-5388. doi: 10.1021/jacs.1c12748. Epub 2022 Mar 2.
7 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
8 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
9 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
10 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
11 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
12 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
13 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
14 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
15 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
16 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
17 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
18 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.