Probe Name	HPAP					Probe Info
Probe concentration	25 uM
Criteria	Quantification: Probe vs (Probe+Competitor)
Quantitative Method	TMT
Competitor Name	Panhematin					Competitor Info
Competitor Concentration	100 uM
In Vivo Experiment Model	Model Type			Cell lysate
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cell lysate (HEK-293T)

Target ID	Target Name					Binding Ratio
LDTP09053	Xyloside xylosyltransferase 1 (XXYLT1)					6.7
LDTP13719	Ubiquitin carboxyl-terminal hydrolase 22 (USP22)					5.9
LDTP05075	Tubulin alpha-4A chain (TUBA4A)					5.7
LDTP03679	Dual specificity protein kinase TTK (TTK)					5.7
LDTP00428	Tripeptidyl-peptidase 1 (TPP1)					5.5
LDTP11874	Thioredoxin-related transmembrane protein 1 (TMX1)					5.2
LDTP06810	Mitochondrial import inner membrane translocase subunit TIM50 (TIMM50)					4.6
LDTP13203	STE20/SPS1-related proline-alanine-rich protein kinase (STK39)					4.4
LDTP06142	Squalene monooxygenase (SQLE)					4.4
LDTP05820	S-phase kinase-associated protein 2 (SKP2)					4.4
LDTP11716	Serine/threonine-protein kinase SIK2 (SIK2)					4.4
LDTP01613	Sphingosine-1-phosphate lyase 1 (SGPL1)					4.4
LDTP12983	Sentrin-specific protease 1 (SENP1)					4.4
LDTP05066	Signal peptidase complex catalytic subunit SEC11A (SEC11A)					4.3
LDTP03139	Succinate dehydrogenase iron-sulfur subunit, mitochondrial (SDHB)					4.2
LDTP01335	Protein SCO1 homolog, mitochondrial (SCO1)					4.2
LDTP08171	Stearoyl-CoA desaturase 5 (SCD5)					4.1
LDTP00344	Stearoyl-CoA desaturase (SCD)					4.0
LDTP16027	Radical S-adenosyl methionine domain-containing protein 1, mitochondrial (RSAD1)					3.9
LDTP07266	ORC ubiquitin ligase 1 (OBI1)					3.8
LDTP15097	All-trans-retinol 13,14-reductase (RETSAT)					3.8
LDTP04892	Ras-related protein Rab-2A (RAB2A)					3.7
LDTP04992	Ras-related protein Rab-11A (RAB11A)					3.7
LDTP04619	5'-AMP-activated protein kinase subunit gamma-1 (PRKAG1)					3.7
LDTP04620	5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2)					3.7
LDTP03510	Peroxiredoxin-6 (PRDX6)					3.7
LDTP00333	Serine/threonine-protein phosphatase 6 catalytic subunit (PPP6C)					3.7
LDTP04353	Protoporphyrinogen oxidase (PPOX)					3.7
LDTP10077	Patatin-like phospholipase domain-containing protein 2 (PNPLA2)					3.7
LDTP15217	Probable proline--tRNA ligase, mitochondrial (PARS2)					3.6
LDTP05313	OTU domain-containing protein 4 (OTUD4)					3.6
LDTP11152	Cancer-related nucleoside-triphosphatase (NTPCR)					3.6
LDTP06455	Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (NSDHL)					3.6
LDTP07591	Neutral cholesterol ester hydrolase 1 (NCEH1)					3.5
LDTP18083	Probable N-acetyltransferase 14 (NAT14)					3.5
LDTP00464	Glutathione S-transferase 3, mitochondrial (MGST3)					3.5
LDTP15155	Thiol S-methyltransferase TMT1B (TMT1B)					3.5
LDTP04103	Dual specificity mitogen-activated protein kinase kinase 3 (MAP2K3)					3.5
LDTP09188	Lysophosphatidylcholine acyltransferase 1 (LPCAT1)					3.5
LDTP10888	Legumain (LGMN)					3.5
LDTP06750	Prolyl 3-hydroxylase 1 (P3H1)					3.5
LDTP06200	Delta(14)-sterol reductase LBR (LBR)					3.4
LDTP12105	L-2-hydroxyglutarate dehydrogenase, mitochondrial (L2HGDH)					3.4
LDTP04435	Interleukin-1 receptor-associated kinase 1 (IRAK1)					3.4
LDTP00885	Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial (IDH3B)					3.4
LDTP07762	Hydroxysteroid dehydrogenase-like protein 2 (HSDL2)					3.4
LDTP06787	Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1)					3.4
LDTP06901	Very-long-chain 3-oxoacyl-CoA reductase (HSD17B12)					3.4
LDTP03540	Heme oxygenase 2 (HMOX2)					3.4
LDTP02359	Heme oxygenase 1 (HMOX1)					3.4
LDTP03993	Probable helicase with zinc finger domain (HELZ)					3.4
LDTP04581	Holocytochrome c-type synthase (HCCS)					3.4
LDTP02696	Glycogen [starch] synthase, muscle (GYS1)					3.3
LDTP01423	Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (GFPT2)					3.3
LDTP09939	Glutaryl-CoA dehydrogenase, mitochondrial (GCDH)					3.3
LDTP01268	2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (GCAT)					3.3
LDTP05623	Polypeptide N-acetylgalactosaminyltransferase 2 (GALNT2)					3.3
LDTP10188	FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1)					3.3
LDTP05468	Folylpolyglutamate synthase, mitochondrial (FPGS)					3.3
LDTP08943	Acyl-coenzyme A diphosphatase FITM2 (FITM2)					3.2
LDTP03198	Ferrochelatase, mitochondrial (FECH)					3.2
LDTP08017	Endoplasmic reticulum metallopeptidase 1 (ERMP1)					3.2
LDTP11174	Deubiquitinase DESI2 (DESI2)					3.2
LDTP00697	Cytochrome b5 type B (CYB5B)					3.2
LDTP06648	Lanosterol 14-alpha demethylase (CYP51A1)					3.2
LDTP01763	Cytochrome b5 (CYB5A)					3.2
LDTP02223	Cathepsin B (CTSB)					3.2
LDTP03257	Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2)					3.1
LDTP07931	Heme A synthase COX15 (COX15)					3.1
LDTP15505	Choline dehydrogenase, mitochondrial (CHDH)					3.1
LDTP10829	Mitochondrial tRNA methylthiotransferase CDK5RAP1 (CDK5RAP1)					3.1
LDTP05247	Cyclin-dependent kinase 17 (CDK17)					3.1
LDTP00249	Cell division cycle 7-related protein kinase (CDC7)					3.1
LDTP08877	3-oxoacyl-[acyl-carrier-protein] reductase (CBR4)					3.1
LDTP19750	RNA ligase 1 (RLIG1)					3.1
LDTP02493	Serine/threonine-protein kinase A-Raf (ARAF)					3.1
LDTP12293	Adipocyte plasma membrane-associated protein (APMAP)					3.1
LDTP11178	Chitobiosyldiphosphodolichol beta-mannosyltransferase (ALG1)					3.1
LDTP03560	Aldehyde dehydrogenase X, mitochondrial (ALDH1B1)					3.1
LDTP04636	Adenylate kinase 2, mitochondrial (AK2)					3.0
LDTP11012	1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1)					3.0
LDTP06905	Acylglycerol kinase, mitochondrial (AGK)					3.0
LDTP10201	Atypical kinase COQ8B, mitochondrial (COQ8B)					3.0
LDTP16274	Acyl-coenzyme A thioesterase 9, mitochondrial (ACOT9)					3.0
LDTP02565	Medium-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADM)					3.0
LDTP08799	Lysophosphatidylserine lipase ABHD12 (ABHD12)					3.0
LDTP05920	Calcium/calmodulin-dependent protein kinase type II subunit gamma (CAMK2G)					3.0

Target ID	Target Name					Binding Ratio
LDTP13824	Voltage-dependent anion-selective channel protein 3 (VDAC3)					6.5
LDTP04071	Voltage-dependent anion-selective channel protein 2 (VDAC2)					6.4
LDTP03132	Voltage-dependent anion-selective channel protein 1 (VDAC1)					6.1
LDTP03546	Translocator protein (TSPO)					5.6
LDTP01748	Mitochondrial import receptor subunit TOM40 homolog (TOMM40)					5.4
LDTP11301	Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1)					5.2
LDTP06945	Sigma intracellular receptor 2 (TMEM97)					5.0
LDTP07477	Transmembrane protein 214 (TMEM214)					5.0
LDTP07058	Transmembrane protein 201 (TMEM201)					4.9
LDTP15948	Transmembrane protein 126A (TMEM126A)					4.8
LDTP11281	Voltage-gated monoatomic cation channel TMEM109 (TMEM109)					4.7
LDTP05875	Transmembrane emp24 domain-containing protein 1 (TMED1)					4.7
LDTP08315	THO complex subunit 6 homolog (THOC6)					4.6
LDTP04033	Translocon-associated protein subunit alpha (SSR1)					4.4
LDTP12090	Sideroflexin-1 (SFXN1)					4.4
LDTP15838	Translocation protein SEC62 (SEC62)					4.3
LDTP04932	Protein transport protein Sec61 subunit alpha isoform 1 (SEC61A1)					4.3
LDTP00358	Succinate dehydrogenase cytochrome b small subunit, mitochondrial (SDHD)					4.3
LDTP11325	Sodium channel modifier 1 (SCNM1)					4.1
LDTP06034	Lysosome membrane protein 2 (SCARB2)					4.0
LDTP00451	Secretory carrier-associated membrane protein 3 (SCAMP3)					4.0
LDTP00547	Secretory carrier-associated membrane protein 2 (SCAMP2)					4.0
LDTP02057	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (RPN1)					3.8
LDTP01060	PRA1 family protein 2 (PRAF2)					3.7
LDTP00562	Membrane-associated progesterone receptor component 2 (PGRMC2)					3.6
LDTP00235	Membrane-associated progesterone receptor component 1 (PGRMC1)					3.6
LDTP10897	Nuclear pore complex protein Nup88 (NUP88)					3.6
LDTP09502	Nuclear pore membrane glycoprotein 210 (NUP210)					3.6
LDTP14235	Mitochondrial carrier homolog 2 (MTCH2)					3.5
LDTP11869	Growth hormone-inducible transmembrane protein (GHITM)					3.3
LDTP05511	Cytoskeleton-associated protein 4 (CKAP4)					3.1
LDTP01352	PRA1 family protein 3 (ARL6IP5)					3.1
LDTP11250	MICOS complex subunit MIC26 (APOO)					3.1
LDTP18526	ATP-binding cassette sub-family F member 2 (ABCF2)					3.0

Target ID	Target Name					Binding Ratio
LDTP01191	Zinc finger protein 217 (ZNF217)					7.0
LDTP06500	Vacuolar protein sorting-associated protein 72 homolog (VPS72)					6.5
LDTP14024	Sal-like protein 2 (SALL2)					3.9
LDTP10945	Pituitary homeobox 2 (PITX2)					3.6
LDTP05097	Pituitary homeobox 1 (PITX1)					3.6
LDTP01645	Nuclear factor of activated T-cells, cytoplasmic 1 (NFATC1)					3.6
LDTP04346	Motor neuron and pancreas homeobox protein 1 (MNX1)					3.5
LDTP03586	Homeobox protein Hox-C9 (HOXC9)					3.4
LDTP02901	Homeobox protein Hox-B6 (HOXB6)					3.4
LDTP04700	Forkhead box protein G1 (FOXG1)					3.2
LDTP13398	Forkhead box protein D3 (FOXD3)					3.2
LDTP05702	Forkhead box protein C1 (FOXC1)					3.2
LDTP04370	ETS domain-containing transcription factor ERF (ERF)					3.2
LDTP10501	DnaJ homolog subfamily C member 1 (DNAJC1)					3.2

Target ID	Target Name					Binding Ratio
LDTP16202	Zinc finger HIT domain-containing protein 2 (ZNHIT2)					10.7
LDTP12033	Zinc finger protein 703 (ZNF703)					10.0
LDTP00507	Zinc finger protein 609 (ZNF609)					9.4
LDTP10295	Zinc finger protein 503 (ZNF503)					8.7
LDTP07223	Zinc finger protein 318 (ZNF318)					8.1
LDTP04143	mRNA decay activator protein ZFP36L2 (ZFP36L2)					6.9
LDTP11233	Tubulin beta-6 chain (TUBB6)					5.9
LDTP02037	Tubulin beta-4A chain (TUBB4A)					5.8
LDTP02205	Tubulin beta chain (TUBB)					5.8
LDTP13323	Multifunctional methyltransferase subunit TRM112-like protein (TRMT112)					5.5
LDTP19963	TraB domain-containing protein (TRABD)					5.5
LDTP06284	TNFAIP3-interacting protein 1 (TNIP1)					5.3
LDTP18373	Thioredoxin-related transmembrane protein 4 (TMX4)					5.2
LDTP03968	Lamina-associated polypeptide 2, isoforms beta/gamma (TMPO)					5.1
LDTP03967	Lamina-associated polypeptide 2, isoform alpha (TMPO)					5.0
LDTP12762	Transmembrane protein 161A (TMEM161A)					4.9
LDTP02887	Transmembrane protein 11, mitochondrial (TMEM11)					4.8
LDTP19831	Protein THEM6 (THEM6)					4.5
LDTP08577	SURP and G-patch domain-containing protein 1 (SUGP1)					4.5
LDTP09142	SUMO-interacting motif-containing protein 1 (SIMC1)					4.4
LDTP06394	SH2 domain-containing adapter protein B (SHB)					4.4
LDTP10830	Selenocysteine insertion sequence-binding protein 2 (SECISBP2)					4.3
LDTP00512	Protein transport protein Sec16A (SEC16A)					4.3
LDTP11915	Protein salvador homolog 1 (SAV1)					4.0
LDTP01522	Reticulon-3 (RTN3)					3.9
LDTP03987	Small ribosomal subunit protein eS27 (RPS27)					3.9
LDTP04975	Small ribosomal subunit protein uS17 (RPS11)					3.9
LDTP05257	Receptor expression-enhancing protein 5 (REEP5)					3.8
LDTP17046	RNA-binding motif, single-stranded-interacting protein 2 (RBMS2)					3.8
LDTP03495	RNA-binding motif, single-stranded-interacting protein 1 (RBMS1)					3.8
LDTP11046	RNA-binding protein 4B (RBM4B)					3.7
LDTP11324	RNA-binding protein 4 (RBM4)					3.7
LDTP18440	Ribonucleoprotein PTB-binding 2 (RAVER2)					3.7
LDTP01130	Pentatricopeptide repeat-containing protein 1, mitochondrial (PTCD1)					3.7
LDTP01021	Perilipin-3 (PLIN3)					3.7
LDTP17355	Pleckstrin homology-like domain family B member 3 (PHLDB3)					3.6
LDTP13630	Neudesin (NENF)					3.6
LDTP10216	RNA-binding protein Musashi homolog 2 (MSI2)					3.5
LDTP14453	RNA-binding protein Musashi homolog 1 (MSI1)					3.5
LDTP15918	Small ribosomal subunit protein mS26 (MRPS26)					3.5
LDTP15922	Large ribosomal subunit protein mL37 (MRPL37)					3.5
LDTP06066	Malectin (MLEC)					3.5
LDTP14165	Melanoma-associated antigen D1 (MAGED1)					3.5
LDTP11115	Leucine zipper putative tumor suppressor 2 (LZTS2)					3.5
LDTP10417	DISP complex protein LRCH3 (LRCH3)					3.5
LDTP13604	Hippocalcin-like protein 4 (HPCAL4)					3.4
LDTP10255	Protein Hook homolog 2 (HOOK2)					3.4
LDTP04500	Heterogeneous nuclear ribonucleoprotein M (HNRNPM)					3.4
LDTP16078	Heme-binding protein 1 (HEBP1)					3.4
LDTP06184	Genetic suppressor element 1 (GSE1)					3.3
LDTP16223	G patch domain-containing protein 8 (GPATCH8)					3.3
LDTP10849	Regulator of microtubule dynamics protein 3 (RMDN3)					3.2
LDTP17721	Refilin-B (RFLNB)					3.2
LDTP11894	DnaJ homolog subfamily C member 5 (DNAJC5)					3.2
LDTP01073	DnaJ homolog subfamily A member 2 (DNAJA2)					3.2
LDTP13402	Dynactin subunit 4 (DCTN4)					3.2
LDTP16129	DDB1- and CUL4-associated factor 16 (DCAF16)					3.2
LDTP04412	Small ribosomal subunit protein mS29 (DAP3)					3.2
LDTP06884	CREB-regulated transcription coactivator 2 (CRTC2)					3.1
LDTP12925	CCR4-NOT transcription complex subunit 2 (CNOT2)					3.1
LDTP02398	CDGSH iron-sulfur domain-containing protein 3, mitochondrial (CISD3)					3.1
LDTP09603	Chromosome transmission fidelity protein 18 homolog (CHTF18)					3.1
LDTP07522	Centrosomal protein of 85 kDa (CEP85)					3.1
LDTP11373	Chromatin remodeling regulator CECR2 (CECR2)					3.1
LDTP19859	Small integral membrane protein 12 (SMIM12)					3.1
LDTP07274	MICOS complex subunit MIC13 (MICOS13)					3.1
LDTP10357	RUS family member 1 (RUSF1)					3.1
LDTP17481	Uncharacterized protein C15orf39 (C15orf39)					3.1
LDTP00555	BET1 homolog (BET1)					3.1
LDTP07738	BCL-6 corepressor (BCOR)					3.1
LDTP13591	A-kinase anchor protein 8-like (AKAP8L)					3.1
LDTP00880	A-kinase anchor protein 8 (AKAP8)					3.1
LDTP07632	Type-1 angiotensin II receptor-associated protein (AGTRAP)					3.0
LDTP04903	Actin-related protein 2 (ACTR2)					3.0

1	A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12. Mass spectrometry data entry: PXD034651