Details of the Target

General Information of Target

Target ID LDTP08877
Target Name 3-oxoacyl-[acyl-carrier-protein] reductase (CBR4)
Gene Name CBR4
Gene ID 84869
Synonyms
SDR45C1; 3-oxoacyl-[acyl-carrier-protein] reductase; EC 1.1.1.100; 3-ketoacyl-[acyl-carrier-protein] reductase beta subunit; KAR beta subunit; Carbonyl reductase family member 4; CBR4; Quinone reductase CBR4; EC 1.6.5.10; Short chain dehydrogenase/reductase family 45C member 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDKVCAVFGGSRGIGRAVAQLMARKGYRLAVIARNLEGAKAAAGDLGGDHLAFSCDVAKE
HDVQNTFEELEKHLGRVNFLVNAAGINRDGLLVRTKTEDMVSQLHTNLLGSMLTCKAAMR
TMIQQQGGSIVNVGSIVGLKGNSGQSVYSASKGGLVGFSRALAKEVARKKIRVNVVAPGF
VHTDMTKDLKEEHLKKNIPLGRFGETIEVAHAVVFLLESPYITGHVLVVDGGLQLIL
Target Bioclass
Enzyme
Family
Short-chain dehydrogenases/reductases (SDR) family
Subcellular location
Mitochondrion matrix
Function
Component of the heterotetramer complex KAR (3-ketoacyl-[acyl carrier protein] reductase or 3-ketoacyl-[ACP] reductase) that forms part of the mitochondrial fatty acid synthase (mtFAS). Beta-subunit of the KAR heterotetramer complex, responsible for the 3-ketoacyl-ACP reductase activity of the mtFAS, reduces 3-oxoacyl-[ACP] to (3R)-hydroxyacyl-[ACP] in a NADPH-dependent manner with no chain length preference, thereby participating in mitochondrial fatty acid biosynthesis. The homotetramer has NADPH-dependent quinone reductase activity (in vitro), hence could play a role in protection against cytotoxicity of exogenous quinones. As a heterotetramer, it can also reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro).
Uniprot ID
Q8N4T8
Ensemble ID
ENST00000306193.8
HGNC ID
HGNC:25891

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2780 SNV: p.L92Ter .
HCT15 SNV: p.G10D .
HT115 SNV: p.K72T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K164(10.00); K196(8.33)  LDD0277  [1]
IPM
 Probe Info  		N.A.  LDD0241  [2]
DBIA
 Probe Info  		C55(1.44)  LDD3312  [3]
HPAP
 Probe Info  		3.11  LDD0062  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C55(0.00); C115(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [5]
TFBX
 Probe Info  		N.A.  LDD0027  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]
NAIA_5
 Probe Info  		C55(0.00); C115(0.00); C5(0.00)  LDD2223  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C5(1.01); C115(1.05)  LDD1508  [9]
 LDCM0226  AC11 HEK-293T C5(0.98)  LDD1509  [9]
 LDCM0237  AC12 HEK-293T C5(0.94); C115(1.20)  LDD1510  [9]
 LDCM0259  AC14 HEK-293T C5(0.97)  LDD1512  [9]
 LDCM0270  AC15 HEK-293T C5(0.95)  LDD1513  [9]
 LDCM0277  AC18 HEK-293T C5(0.95); C115(0.98)  LDD1516  [9]
 LDCM0278  AC19 HEK-293T C5(1.06)  LDD1517  [9]
 LDCM0279  AC2 HEK-293T C5(0.87); C115(0.98)  LDD1518  [9]
 LDCM0280  AC20 HEK-293T C5(0.97); C115(1.01)  LDD1519  [9]
 LDCM0281  AC21 HEK-293T C5(1.02)  LDD1520  [9]
 LDCM0282  AC22 HEK-293T C5(1.04)  LDD1521  [9]
 LDCM0283  AC23 HEK-293T C5(0.99)  LDD1522  [9]
 LDCM0284  AC24 HEK-293T C5(0.91)  LDD1523  [9]
 LDCM0286  AC26 HEK-293T C5(0.97); C115(0.89)  LDD1525  [9]
 LDCM0287  AC27 HEK-293T C5(0.99)  LDD1526  [9]
 LDCM0288  AC28 HEK-293T C5(0.98); C115(0.88)  LDD1527  [9]
 LDCM0289  AC29 HEK-293T C5(0.96)  LDD1528  [9]
 LDCM0290  AC3 HEK-293T C5(0.99)  LDD1529  [9]
 LDCM0291  AC30 HEK-293T C5(1.01)  LDD1530  [9]
 LDCM0292  AC31 HEK-293T C5(0.99)  LDD1531  [9]
 LDCM0293  AC32 HEK-293T C5(1.02)  LDD1532  [9]
 LDCM0295  AC34 HEK-293T C5(0.91); C115(1.11)  LDD1534  [9]
 LDCM0296  AC35 HEK-293T C5(0.99)  LDD1535  [9]
 LDCM0297  AC36 HEK-293T C5(0.94); C115(0.97)  LDD1536  [9]
 LDCM0298  AC37 HEK-293T C5(1.02)  LDD1537  [9]
 LDCM0299  AC38 HEK-293T C5(0.94)  LDD1538  [9]
 LDCM0300  AC39 HEK-293T C5(0.96)  LDD1539  [9]
 LDCM0301  AC4 HEK-293T C5(0.95); C115(1.12)  LDD1540  [9]
 LDCM0302  AC40 HEK-293T C5(0.92)  LDD1541  [9]
 LDCM0304  AC42 HEK-293T C5(0.95); C115(1.07)  LDD1543  [9]
 LDCM0305  AC43 HEK-293T C5(0.99)  LDD1544  [9]
 LDCM0306  AC44 HEK-293T C5(0.94); C115(1.15)  LDD1545  [9]
 LDCM0307  AC45 HEK-293T C5(1.02)  LDD1546  [9]
 LDCM0308  AC46 HEK-293T C5(0.95)  LDD1547  [9]
 LDCM0309  AC47 HEK-293T C5(1.01)  LDD1548  [9]
 LDCM0310  AC48 HEK-293T C5(0.91)  LDD1549  [9]
 LDCM0312  AC5 HEK-293T C5(1.00)  LDD1551  [9]
 LDCM0313  AC50 HEK-293T C5(1.00); C115(1.02)  LDD1552  [9]
 LDCM0314  AC51 HEK-293T C5(0.97)  LDD1553  [9]
 LDCM0315  AC52 HEK-293T C5(0.95); C115(1.06)  LDD1554  [9]
 LDCM0316  AC53 HEK-293T C5(0.96)  LDD1555  [9]
 LDCM0317  AC54 HEK-293T C5(1.00)  LDD1556  [9]
 LDCM0318  AC55 HEK-293T C5(0.93)  LDD1557  [9]
 LDCM0319  AC56 HEK-293T C5(0.93)  LDD1558  [9]
 LDCM0321  AC58 HEK-293T C5(1.00); C115(0.97)  LDD1560  [9]
 LDCM0322  AC59 HEK-293T C5(0.97)  LDD1561  [9]
 LDCM0323  AC6 HEK-293T C5(0.97)  LDD1562  [9]
 LDCM0324  AC60 HEK-293T C5(1.00); C115(1.07)  LDD1563  [9]
 LDCM0325  AC61 HEK-293T C5(1.02)  LDD1564  [9]
 LDCM0326  AC62 HEK-293T C5(0.96)  LDD1565  [9]
 LDCM0327  AC63 HEK-293T C5(0.92)  LDD1566  [9]
 LDCM0328  AC64 HEK-293T C5(0.92)  LDD1567  [9]
 LDCM0334  AC7 HEK-293T C5(0.93)  LDD1568  [9]
 LDCM0345  AC8 HEK-293T C5(0.94)  LDD1569  [9]
 LDCM0248  AKOS034007472 HEK-293T C5(1.02)  LDD1511  [9]
 LDCM0275  AKOS034007705 HEK-293T C5(0.98)  LDD1514  [9]
 LDCM0632  CL-Sc Hep-G2 C5(1.36)  LDD2227  [8]
 LDCM0368  CL10 HEK-293T C5(1.00)  LDD1572  [9]
 LDCM0369  CL100 HEK-293T C5(1.05); C115(0.99)  LDD1573  [9]
 LDCM0373  CL104 HEK-293T C5(0.91); C115(1.13)  LDD1577  [9]
 LDCM0377  CL108 HEK-293T C5(0.95); C115(0.97)  LDD1581  [9]
 LDCM0379  CL11 HEK-293T C5(0.94)  LDD1583  [9]
 LDCM0382  CL112 HEK-293T C5(1.02); C115(0.98)  LDD1586  [9]
 LDCM0386  CL116 HEK-293T C5(0.94); C115(1.01)  LDD1590  [9]
 LDCM0390  CL12 HEK-293T C5(0.92)  LDD1594  [9]
 LDCM0391  CL120 HEK-293T C5(1.00); C115(1.09)  LDD1595  [9]
 LDCM0395  CL124 HEK-293T C5(0.93); C115(1.10)  LDD1599  [9]
 LDCM0399  CL128 HEK-293T C5(0.94); C115(0.94)  LDD1603  [9]
 LDCM0403  CL16 HEK-293T C5(0.89); C115(1.06)  LDD1607  [9]
 LDCM0405  CL18 HEK-293T C5(0.97); C115(1.07)  LDD1609  [9]
 LDCM0406  CL19 HEK-293T C5(1.03)  LDD1610  [9]
 LDCM0408  CL20 HEK-293T C5(0.93); C115(1.46)  LDD1612  [9]
 LDCM0409  CL21 HEK-293T C5(1.05)  LDD1613  [9]
 LDCM0410  CL22 HEK-293T C5(0.99)  LDD1614  [9]
 LDCM0411  CL23 HEK-293T C5(0.97)  LDD1615  [9]
 LDCM0412  CL24 HEK-293T C5(0.90)  LDD1616  [9]
 LDCM0416  CL28 HEK-293T C5(0.96); C115(1.06)  LDD1620  [9]
 LDCM0419  CL30 HEK-293T C5(0.89); C115(1.09)  LDD1623  [9]
 LDCM0420  CL31 HEK-293T C5(1.02)  LDD1624  [9]
 LDCM0421  CL32 HEK-293T C5(0.93); C115(1.27)  LDD1625  [9]
 LDCM0422  CL33 HEK-293T C5(1.15)  LDD1626  [9]
 LDCM0423  CL34 HEK-293T C5(1.04)  LDD1627  [9]
 LDCM0424  CL35 HEK-293T C5(1.02)  LDD1628  [9]
 LDCM0425  CL36 HEK-293T C5(1.02)  LDD1629  [9]
 LDCM0429  CL4 HEK-293T C5(0.93); C115(0.99)  LDD1633  [9]
 LDCM0430  CL40 HEK-293T C5(1.00); C115(0.99)  LDD1634  [9]
 LDCM0432  CL42 HEK-293T C5(1.00); C115(1.16)  LDD1636  [9]
 LDCM0433  CL43 HEK-293T C5(0.98)  LDD1637  [9]
 LDCM0434  CL44 HEK-293T C5(0.89); C115(1.09)  LDD1638  [9]
 LDCM0435  CL45 HEK-293T C5(1.08)  LDD1639  [9]
 LDCM0436  CL46 HEK-293T C5(1.11)  LDD1640  [9]
 LDCM0437  CL47 HEK-293T C5(1.03)  LDD1641  [9]
 LDCM0438  CL48 HEK-293T C5(1.05)  LDD1642  [9]
 LDCM0443  CL52 HEK-293T C5(0.94); C115(1.06)  LDD1646  [9]
 LDCM0445  CL54 HEK-293T C5(1.02); C115(1.10)  LDD1648  [9]
 LDCM0446  CL55 HEK-293T C5(1.03)  LDD1649  [9]
 LDCM0447  CL56 HEK-293T C5(0.96); C115(1.24)  LDD1650  [9]
 LDCM0448  CL57 HEK-293T C5(1.01)  LDD1651  [9]
 LDCM0449  CL58 HEK-293T C5(1.11)  LDD1652  [9]
 LDCM0450  CL59 HEK-293T C5(1.00)  LDD1653  [9]
 LDCM0451  CL6 HEK-293T C5(0.90); C115(1.12)  LDD1654  [9]
 LDCM0452  CL60 HEK-293T C5(0.99)  LDD1655  [9]
 LDCM0456  CL64 HEK-293T C5(0.95); C115(1.07)  LDD1659  [9]
 LDCM0458  CL66 HEK-293T C5(0.89); C115(1.00)  LDD1661  [9]
 LDCM0459  CL67 HEK-293T C5(0.98)  LDD1662  [9]
 LDCM0460  CL68 HEK-293T C5(0.99); C115(1.11)  LDD1663  [9]
 LDCM0461  CL69 HEK-293T C5(1.04)  LDD1664  [9]
 LDCM0462  CL7 HEK-293T C5(1.04)  LDD1665  [9]
 LDCM0463  CL70 HEK-293T C5(1.00)  LDD1666  [9]
 LDCM0464  CL71 HEK-293T C5(1.11)  LDD1667  [9]
 LDCM0465  CL72 HEK-293T C5(0.96)  LDD1668  [9]
 LDCM0469  CL76 HEK-293T C5(0.89); C115(1.02)  LDD1672  [9]
 LDCM0471  CL78 HEK-293T C5(0.94); C115(1.02)  LDD1674  [9]
 LDCM0472  CL79 HEK-293T C5(1.02)  LDD1675  [9]
 LDCM0473  CL8 HEK-293T C5(0.94); C115(0.85)  LDD1676  [9]
 LDCM0474  CL80 HEK-293T C5(1.00); C115(1.14)  LDD1677  [9]
 LDCM0475  CL81 HEK-293T C5(1.01)  LDD1678  [9]
 LDCM0476  CL82 HEK-293T C5(1.00)  LDD1679  [9]
 LDCM0477  CL83 HEK-293T C5(1.05)  LDD1680  [9]
 LDCM0478  CL84 HEK-293T C5(1.01)  LDD1681  [9]
 LDCM0482  CL88 HEK-293T C5(0.97); C115(1.06)  LDD1685  [9]
 LDCM0484  CL9 HEK-293T C5(1.00)  LDD1687  [9]
 LDCM0485  CL90 HEK-293T C5(1.03); C115(1.16)  LDD1688  [9]
 LDCM0486  CL91 HEK-293T C5(0.96)  LDD1689  [9]
 LDCM0487  CL92 HEK-293T C5(0.94); C115(1.04)  LDD1690  [9]
 LDCM0488  CL93 HEK-293T C5(1.04)  LDD1691  [9]
 LDCM0489  CL94 HEK-293T C5(0.98)  LDD1692  [9]
 LDCM0490  CL95 HEK-293T C5(1.01)  LDD1693  [9]
 LDCM0491  CL96 HEK-293T C5(0.99)  LDD1694  [9]
 LDCM0625  F8 Ramos C55(0.42); C115(1.14)  LDD2187  [10]
 LDCM0572  Fragment10 Ramos C55(0.44)  LDD2189  [10]
 LDCM0573  Fragment11 Ramos C55(3.29); C115(1.06)  LDD2190  [10]
 LDCM0574  Fragment12 Ramos C55(0.47)  LDD2191  [10]
 LDCM0575  Fragment13 Ramos C55(0.65)  LDD2192  [10]
 LDCM0576  Fragment14 Ramos C55(1.21); C115(0.89)  LDD2193  [10]
 LDCM0579  Fragment20 Ramos C55(0.37)  LDD2194  [10]
 LDCM0580  Fragment21 Ramos C55(1.09)  LDD2195  [10]
 LDCM0578  Fragment27 Ramos C55(0.86)  LDD2197  [10]
 LDCM0586  Fragment28 Ramos C115(1.01)  LDD2198  [10]
 LDCM0588  Fragment30 Ramos C55(1.16)  LDD2199  [10]
 LDCM0590  Fragment32 Ramos C55(0.53)  LDD2201  [10]
 LDCM0468  Fragment33 Ramos C55(0.99)  LDD2202  [10]
 LDCM0596  Fragment38 Ramos C55(1.06)  LDD2203  [10]
 LDCM0566  Fragment4 Ramos C55(1.84); C115(0.57)  LDD2184  [10]
 LDCM0610  Fragment52 Ramos C55(1.07)  LDD2204  [10]
 LDCM0614  Fragment56 Ramos C55(0.73)  LDD2205  [10]
 LDCM0569  Fragment7 Ramos C55(1.52); C115(0.41)  LDD2186  [10]
 LDCM0571  Fragment9 Ramos C55(0.46)  LDD2188  [10]
 LDCM0022  KB02 Ramos C55(0.42); C115(0.23)  LDD2182  [10]
 LDCM0023  KB03 Ramos C55(1.05); C115(0.40)  LDD2183  [10]
 LDCM0024  KB05 HMCB C55(1.44)  LDD3312  [3]
 LDCM0014  Panhematin HEK-293T 3.11  LDD0062  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein ZXDC (ZXDC) ZXD family Q2QGD7

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
10 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578