Details of the Target

General Information of Target

Target ID LDTP03987
Target Name Small ribosomal subunit protein eS27 (RPS27)
Gene Name RPS27
Gene ID 6232
Synonyms
MPS1; Small ribosomal subunit protein eS27; 40S ribosomal protein S27; Metallopan-stimulin 1; MPS-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCS
TVLCQPTGGKARLTEGCSFRRKQH
Target Bioclass
Other
Family
Eukaryotic ribosomal protein eS27 family
Subcellular location
Cytoplasm
Function
Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for proper rRNA processing and maturation of 18S rRNAs. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Uniprot ID
P42677
Ensemble ID
ENST00000651669.1
HGNC ID
HGNC:10416
ChEMBL ID
CHEMBL1932893

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 37 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		14.82  LDD0402  [1]
P1
 Probe Info  		1.81  LDD0448  [2]
P2
 Probe Info  		1.66  LDD0449  [2]
P8
 Probe Info  		1.87  LDD0451  [2]
CY-1
 Probe Info  		6.12  LDD0243  [3]
CY4
 Probe Info  		4.17  LDD0244  [3]
N1
 Probe Info  		6.28  LDD0242  [3]
TH211
 Probe Info  		Y31(13.36)  LDD0260  [4]
TH214
 Probe Info  		Y31(13.92)  LDD0258  [4]
TH216
 Probe Info  		Y31(15.16)  LDD0259  [4]
YN-4
 Probe Info  		100.00  LDD0445  [5]
STPyne
 Probe Info  		K16(6.92)  LDD0277  [6]
BTD
 Probe Info  		C77(4.75)  LDD1699  [7]
ONAyne
 Probe Info  		N.A.  LDD0273  [6]
AF-1
 Probe Info  		1.64  LDD0421  [8]
HPAP
 Probe Info  		3.87  LDD0062  [9]
HHS-482
 Probe Info  		Y31(0.07)  LDD0289  [10]
DBIA
 Probe Info  		C77(1.51)  LDD0078  [11]
5E-2FA
 Probe Info  		N.A.  LDD2235  [12]
ATP probe
 Probe Info  		K5(0.00); K16(0.00)  LDD0199  [13]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [14]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [15]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [16]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [16]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [14]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [17]
NAIA_4
 Probe Info  		N.A.  LDD2226  [18]
TFBX
 Probe Info  		N.A.  LDD0027  [17]
Compound 10
 Probe Info  		N.A.  LDD2216  [19]
IPM
 Probe Info  		N.A.  LDD2156  [20]
NHS
 Probe Info  		N.A.  LDD0010  [21]
OSF
 Probe Info  		N.A.  LDD0029  [22]
SF
 Probe Info  		N.A.  LDD0028  [22]
Acrolein
 Probe Info  		N.A.  LDD0217  [23]
AOyne
 Probe Info  		15.00  LDD0443  [24]
NAIA_5
 Probe Info  		N.A.  LDD2223  [18]
HHS-475
 Probe Info  		Y31(1.03)  LDD2238  [25]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0166  Afatinib A431 1.64  LDD0421  [8]
 LDCM0156  Aniline NCI-H1299 11.63  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C77(1.51)  LDD0078  [11]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [23]
 LDCM0632  CL-Sc Hep-G2 C77(1.74); C77(1.16); C77(0.93)  LDD2227  [18]
 LDCM0634  CY-0357 Hep-G2 C77(0.63)  LDD2228  [18]
 LDCM0625  F8 Ramos C77(1.43)  LDD2187  [26]
 LDCM0572  Fragment10 MDA-MB-231 C77(3.17)  LDD1389  [27]
 LDCM0573  Fragment11 MDA-MB-231 C77(3.27)  LDD1391  [27]
 LDCM0574  Fragment12 Ramos C77(2.19)  LDD1394  [27]
 LDCM0575  Fragment13 MDA-MB-231 C77(1.45)  LDD1395  [27]
 LDCM0576  Fragment14 MDA-MB-231 C77(1.42)  LDD1397  [27]
 LDCM0577  Fragment15 Ramos C77(1.09)  LDD1400  [27]
 LDCM0579  Fragment20 MDA-MB-231 C77(1.26)  LDD1402  [27]
 LDCM0580  Fragment21 MDA-MB-231 C77(1.66)  LDD1404  [27]
 LDCM0581  Fragment22 MDA-MB-231 C77(1.54)  LDD1406  [27]
 LDCM0582  Fragment23 MDA-MB-231 C77(1.31)  LDD1408  [27]
 LDCM0584  Fragment25 MDA-MB-231 C77(0.96)  LDD1411  [27]
 LDCM0585  Fragment26 Ramos C77(0.96)  LDD1412  [27]
 LDCM0578  Fragment27 MDA-MB-231 C77(0.86)  LDD1401  [27]
 LDCM0586  Fragment28 MDA-MB-231 C77(1.14)  LDD1415  [27]
 LDCM0587  Fragment29 MDA-MB-231 C77(1.29)  LDD1417  [27]
 LDCM0588  Fragment30 MDA-MB-231 C77(1.34)  LDD1419  [27]
 LDCM0589  Fragment31 Ramos C77(0.86)  LDD2200  [26]
 LDCM0590  Fragment32 MDA-MB-231 C77(4.53)  LDD1423  [27]
 LDCM0468  Fragment33 MDA-MB-231 C77(1.27)  LDD1425  [27]
 LDCM0592  Fragment34 MDA-MB-231 C77(1.29)  LDD1427  [27]
 LDCM0593  Fragment35 Ramos C77(0.86)  LDD1430  [27]
 LDCM0594  Fragment36 MDA-MB-231 C77(1.78)  LDD1431  [27]
 LDCM0595  Fragment37 Ramos C77(0.85)  LDD1432  [27]
 LDCM0596  Fragment38 MDA-MB-231 C77(1.00)  LDD1433  [27]
 LDCM0566  Fragment4 MDA-MB-231 C77(4.42)  LDD1378  [27]
 LDCM0598  Fragment40 MDA-MB-231 C77(0.83)  LDD1436  [27]
 LDCM0599  Fragment41 MDA-MB-231 C77(1.24)  LDD1438  [27]
 LDCM0600  Fragment42 Ramos C77(1.14)  LDD1440  [27]
 LDCM0601  Fragment43 MDA-MB-231 C77(2.49)  LDD1441  [27]
 LDCM0602  Fragment44 MDA-MB-231 C77(0.77)  LDD1443  [27]
 LDCM0603  Fragment45 MDA-MB-231 C77(6.20)  LDD1444  [27]
 LDCM0604  Fragment46 MDA-MB-231 C77(0.89)  LDD1445  [27]
 LDCM0605  Fragment47 MDA-MB-231 C77(1.02)  LDD1446  [27]
 LDCM0606  Fragment48 MDA-MB-231 C77(1.16)  LDD1447  [27]
 LDCM0608  Fragment50 MDA-MB-231 C77(5.06)  LDD1449  [27]
 LDCM0427  Fragment51 MDA-MB-231 C77(0.98)  LDD1450  [27]
 LDCM0610  Fragment52 MDA-MB-231 C77(1.43)  LDD1452  [27]
 LDCM0611  Fragment53 MDA-MB-231 C77(1.07)  LDD1454  [27]
 LDCM0612  Fragment54 MDA-MB-231 C77(0.96)  LDD1456  [27]
 LDCM0613  Fragment55 MDA-MB-231 C77(1.12)  LDD1457  [27]
 LDCM0614  Fragment56 MDA-MB-231 C77(1.13)  LDD1458  [27]
 LDCM0569  Fragment7 Ramos C77(1.34)  LDD2186  [26]
 LDCM0570  Fragment8 MDA-MB-231 C77(3.07)  LDD1385  [27]
 LDCM0571  Fragment9 MDA-MB-231 C77(1.22)  LDD1387  [27]
 LDCM0015  HNE MDA-MB-231 C77(0.90)  LDD0346  [26]
 LDCM0107  IAA HeLa N.A.  LDD0221  [23]
 LDCM0127  JWB152 DM93 Y31(0.07)  LDD0289  [10]
 LDCM0128  JWB198 DM93 Y31(0.17)  LDD0290  [10]
 LDCM0022  KB02 MDA-MB-231 C77(8.33)  LDD1374  [27]
 LDCM0023  KB03 MDA-MB-231 C77(8.51)  LDD1376  [27]
 LDCM0024  KB05 Ramos C77(2.70)  LDD1381  [27]
 LDCM0109  NEM HeLa H9(0.00); K5(0.00)  LDD0223  [23]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C77(1.26)  LDD2090  [7]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C77(0.98)  LDD2111  [7]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C77(0.85)  LDD2125  [7]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C77(1.16)  LDD2136  [7]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C77(0.91)  LDD2137  [7]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C77(6.46)  LDD2145  [7]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C77(0.65)  LDD2148  [7]
 LDCM0014  Panhematin HEK-293T 3.87  LDD0062  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
RecQ-like DNA helicase BLM (BLM) Helicase family P54132
E3 ubiquitin-protein ligase Mdm2 (MDM2) MDM2/MDM4 family Q00987
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) TKL Ser/Thr protein kinase family Q5S007
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proteasome activator complex subunit 3 (PSME3) PA28 family P61289

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
6 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
7 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
8 Minimalist linkers suitable for irreversible inhibitors in simultaneous proteome profiling, live-cell imaging and drug screening. Chem Commun (Camb). 2019 Jan 15;55(6):834-837. doi: 10.1039/c8cc08685k.
9 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
10 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
11 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
12 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
13 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
14 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
15 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
16 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
17 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
18 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
19 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
20 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
21 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
22 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
23 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
24 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
25 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
26 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
27 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.