Probe Name	AF-1					Probe Info
Probe concentration	0.1 uM
Criteria	Quantification: Probe vs (Probe+Competitor)
Quantitative Method	LFQ
Competitor Name	Afatinib					Competitor Info
Competitor Concentration	0.5 uM
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Skin
	Disease Model		Skin squamous cell carcinoma [ICD-11:2C31]
	Model Name		Human skin squamous cell carcinoma cells (A431)

Target ID	Target Name					Binding Ratio
LDTP02541	Heat shock cognate 71 kDa protein (HSPA8)					10.0
LDTP11733	Ras-related protein Rab-1B (RAB1B)					10.0
LDTP12867	Very-long-chain enoyl-CoA reductase (TECR)					10.0
LDTP01785	Epidermal growth factor receptor (EGFR)					3.6
LDTP03644	Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1)					3.4
LDTP01783	Aspartate aminotransferase, mitochondrial (GOT2)					2.7
LDTP11311	Very long chain fatty acid elongase 1 (ELOVL1)					2.4
LDTP07504	Lysophospholipid acyltransferase 5 (LPCAT3)					2.3
LDTP05885	DNA topoisomerase 3-alpha (TOP3A)					2.3
LDTP06067	Tubulin--tyrosine ligase-like protein 12 (TTLL12)					2.2
LDTP02672	Elongation factor 2 (EEF2)					2.2
LDTP09539	Dual specificity protein phosphatase 19 (DUSP19)					2.0
LDTP14229	Signal peptidase complex subunit 1 (SPCS1)					1.9
LDTP02622	Creatine kinase U-type, mitochondrial (CKMT1A; CKMT1B)					1.6
LDTP03242	Adenosylhomocysteinase (AHCY)					1.5

Target ID	Target Name					Binding Ratio
LDTP12030	Splicing factor, arginine/serine-rich 19 (SCAF1)					10.0
LDTP05273	Spectrin beta chain, non-erythrocytic 1 (SPTBN1)					1.7

Target ID	Target Name					Binding Ratio
LDTP18926	Zinc finger protein 736 (ZNF736)					6.0
LDTP18926	Zinc finger protein 736 (ZNF736)					2.2

Target ID	Target Name					Binding Ratio
LDTP04993	Eukaryotic peptide chain release factor subunit 1 (ETF1)					10.0
LDTP06086	Lymphocyte antigen 6D (LY6D)					10.0
LDTP04110	Small ribosomal subunit protein uS7 (RPS5)					10.0
LDTP02779	Ezrin (EZR)					4.5
LDTP02845	Histone H1.2 (H1-2)					3.6
LDTP01534	Luc7-like protein 3 (LUC7L3)					3.3
LDTP06220	LIM and SH3 domain protein 1 (LASP1)					3.3
LDTP07136	Keratinocyte proline-rich protein (KPRP)					3.0
LDTP02674	Keratin, type I cytoskeletal 10 (KRT10)					3.0
LDTP03792	Keratin, type II cytoskeletal 2 epidermal (KRT2)					2.8
LDTP04913	Small ribosomal subunit protein eS1 (RPS3A)					2.5
LDTP04226	Paxillin (PXN)					2.5
LDTP04972	Small ribosomal subunit protein uS13 (RPS18)					2.3
LDTP04880	Small ribosomal subunit protein uS10 (RPS20)					2.1
LDTP02676	Keratin, type II cytoskeletal 5 (KRT5)					2.0
LDTP04510	Rho GDP-dissociation inhibitor 1 (ARHGDIA)					1.9
LDTP05021	Large ribosomal subunit protein eL30 (RPL30)					1.9
LDTP03076	Endothelin-2 (EDN2)					1.9
LDTP02031	Keratin, type II cytoskeletal 1 (KRT1)					1.9
LDTP04233	Large ribosomal subunit protein eL34 (RPL34)					1.7
LDTP02302	Small ribosomal subunit protein eS17 (RPS17)					1.7
LDTP00827	Pleiotropic regulator 1 (PLRG1)					1.7
LDTP03987	Small ribosomal subunit protein eS27 (RPS27)					1.6
LDTP05509	Large ribosomal subunit protein eL18 (RPL18)					1.6
LDTP05024	Large ribosomal subunit protein uL1 (RPL10A)					1.6
LDTP04990	Large ribosomal subunit protein eL8 (RPL7A)					1.6
LDTP02944	Large ribosomal subunit protein uL30 (RPL7)					1.6
LDTP03354	Large ribosomal subunit protein eL13 (RPL13)					1.5
LDTP05033	Ubiquitin-ribosomal protein eS31 fusion protein (RPS27A)					1.5

1	Minimalist linkers suitable for irreversible inhibitors in simultaneous proteome profiling, live-cell imaging and drug screening. Chem Commun (Camb). 2019 Jan 15;55(6):834-837. doi: 10.1039/c8cc08685k.