Details of the Target

General Information of Target

Target ID LDTP04972
Target Name Small ribosomal subunit protein uS13 (RPS18)
Gene Name RPS18
Gene ID 6222
Synonyms
D6S218E; Small ribosomal subunit protein uS13; 40S ribosomal protein S18; Ke-3; Ke3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELT
EDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAH
RGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK
Target Bioclass
Other
Family
Universal ribosomal protein uS13 family
Subcellular location
Cytoplasm
Function Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Uniprot ID
P62269
Ensemble ID
ENST00000211372.9
HGNC ID
HGNC:10401

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 27 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P2
 Probe Info  		1.90  LDD0449  [1]
P8
 Probe Info  		2.41  LDD0451  [1]
A-EBA
 Probe Info  		3.74  LDD0215  [2]
C-Sul
 Probe Info  		6.36  LDD0066  [3]
TH211
 Probe Info  		Y77(17.57); Y40(15.24)  LDD0257  [4]
TH214
 Probe Info  		Y40(9.89); Y77(8.36)  LDD0258  [4]
TH216
 Probe Info  		Y40(20.00); Y95(8.93)  LDD0259  [4]
YN-1
 Probe Info  		100.00  LDD0444  [5]
ONAyne
 Probe Info  		K137(0.00); K47(0.00); K106(0.00); K150(0.00)  LDD0273  [6]
OPA-S-S-alkyne
 Probe Info  		K78(0.30); K54(1.41); K47(1.41); K106(1.85)  LDD3494  [7]
AF-1
 Probe Info  		2.27  LDD0421  [8]
HPAP
 Probe Info  		3.83  LDD0064  [9]
HHS-482
 Probe Info  		Y40(0.82); Y77(4.03); Y95(1.28)  LDD0285  [10]
HHS-475
 Probe Info  		Y77(2.15)  LDD0267  [11]
Acrolein
 Probe Info  		H42(0.00); H125(0.00)  LDD0221  [12]
ATP probe
 Probe Info  		K94(0.00); K78(0.00); K47(0.00)  LDD0199  [13]
ATP probe
 Probe Info  		K8(0.00); K25(0.00); K106(0.00)  LDD0035  [14]
AZ-9
 Probe Info  		N.A.  LDD0395  [15]
1d-yne
 Probe Info  		N.A.  LDD0356  [16]
NHS
 Probe Info  		N.A.  LDD0010  [17]
OSF
 Probe Info  		Y40(0.00); H42(0.00)  LDD0029  [18]
SF
 Probe Info  		K47(0.00); Y95(0.00); K25(0.00); K54(0.00)  LDD0028  [18]
STPyne
 Probe Info  		N.A.  LDD0009  [17]
Ox-W18
 Probe Info  		W82(0.00); W127(0.00)  LDD2175  [19]
1c-yne
 Probe Info  		N.A.  LDD0228  [16]
Crotonaldehyde
 Probe Info  		H42(0.00); H125(0.00)  LDD0219  [12]
W1
 Probe Info  		Y40(0.00); H42(0.00)  LDD0236  [20]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
JN0003
 Probe Info  		5.94  LDD0469  [21]
STS-2
 Probe Info  		1.52  LDD0138  [22]
Diazir
 Probe Info  		N.A.  LDD0011  [17]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0166  Afatinib A431 2.27  LDD0421  [8]
 LDCM0108  Chloroacetamide HeLa H42(0.00); H125(0.00)  LDD0222  [12]
 LDCM0119  HHS-0401 DM93 Y77(2.15)  LDD0267  [11]
 LDCM0107  IAA HeLa H42(0.00); H125(0.00)  LDD0221  [12]
 LDCM0123  JWB131 DM93 Y40(0.82); Y77(4.03); Y95(1.28)  LDD0285  [10]
 LDCM0124  JWB142 DM93 Y40(0.74); Y77(0.76); Y95(0.78)  LDD0286  [10]
 LDCM0125  JWB146 DM93 Y40(2.20); Y77(2.43); Y95(1.63)  LDD0287  [10]
 LDCM0126  JWB150 DM93 Y40(7.17); Y77(1.80); Y95(0.84)  LDD0288  [10]
 LDCM0127  JWB152 DM93 Y40(4.45); Y77(0.05); Y95(9.94)  LDD0289  [10]
 LDCM0128  JWB198 DM93 Y40(2.10); Y95(1.81)  LDD0290  [10]
 LDCM0129  JWB202 DM93 Y40(0.32); Y77(1.05); Y95(0.65)  LDD0291  [10]
 LDCM0130  JWB211 DM93 Y40(1.70); Y77(0.05); Y95(6.60)  LDD0292  [10]
 LDCM0109  NEM HeLa H42(0.00); H125(0.00)  LDD0223  [12]
 LDCM0014  Panhematin hPBMC 3.83  LDD0064  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) TKL Ser/Thr protein kinase family Q5S007

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Artenimol . DB11638

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
3 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
6 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
7 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
8 Minimalist linkers suitable for irreversible inhibitors in simultaneous proteome profiling, live-cell imaging and drug screening. Chem Commun (Camb). 2019 Jan 15;55(6):834-837. doi: 10.1039/c8cc08685k.
9 A Chemical Proteomic Map of Heme-Protein Interactions. J Am Chem Soc. 2022 Aug 24;144(33):15013-15019. doi: 10.1021/jacs.2c06104. Epub 2022 Aug 12.
Mass spectrometry data entry: PXD034651
10 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
11 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
12 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
13 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
14 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
15 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
16 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
17 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
18 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
19 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
20 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
21 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
22 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.