Details of the Probe

General Information of Probe

Probe ID
LDPC0248
Probe Name
P2
Synonyms
1233382-94-9;N-(4-ethynylphenyl)acrylamide;N-(4-ethynylphenyl)-2-propenamide;N-(4-ethynylphenyl)prop-2-enamide;SCHEMBL1497375;TYWHWZBTVJILQU-UHFFFAOYSA-N;2-Propenamide, N-(4-ethynylphenyl)-;AKOS034076391;AS-84042;DB-114886;E77741;EN300-708694;Z1509200130
Probe Type
ABPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 171.19
Lipid-water partition coefficient (xlogp) 1.9
Rotatable Bond Count (rotbonds) 3
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 1
Chemical Identifiers
Formula
C11H9NO
IUPAC Name
N-(4-ethynylphenyl)prop-2-enamide
Canonical SMILES
C=CC(=O)NC1=CC=C(C=C1)C#C
InChI
InChI=1S/C11H9NO/c1-3-9-5-7-10(8-6-9)12-11(13)4-2/h1,4-8H,2H2,(H,12,13)
InChIKey
TYWHWZBTVJILQU-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

47 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
60 kDa heat shock protein, mitochondrial (HSPD1) LDTP02522 2.84  LDD0449 
Actin, alpha skeletal muscle (ACTA1) LDTP05073 1.67  LDD0449 
Actin, cytoplasmic 1 (ACTB) LDTP04876 10.00  LDD0453 
Actin, cytoplasmic 2 (ACTG1) LDTP05059 1.83  LDD0449 
Adenosylhomocysteinase (AHCY) LDTP03242 2.22  LDD0449 
Aldo-keto reductase family 1 member C3 (AKR1C3) LDTP03976 1.51  LDD0453 
Alpha-enolase (ENO1) LDTP02159 1.69  LDD0449 
Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS) LDTP03159 10.00  LDD0449 
Cell division control protein 42 homolog (CDC42) LDTP04886 10.00  LDD0453 
D-3-phosphoglycerate dehydrogenase (PHGDH) LDTP00701 10.00  LDD0449 
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial (DUT) LDTP03669 2.74  LDD0449 
Dermcidin (DCD) LDTP05171 2.02  LDD0449 
DNA-dependent protein kinase catalytic subunit (PRKDC) LDTP05136 10.00  LDD0449 
E3 ubiquitin-protein ligase RNF180 (RNF180) LDTP08190 4.82  LDD0453 
EH domain-containing protein 2 (EHD2) LDTP12923 4.08  LDD0449 
Elongation factor 2 (EEF2) LDTP02672 1.62  LDD0449 
Endoplasmic reticulum chaperone BiP (HSPA5) LDTP02534 10.00  LDD0449 
Epidermal growth factor receptor (EGFR) LDTP01785 3.94  LDD0449 
Eukaryotic initiation factor 4A-I (EIF4A1) LDTP04878 1.77  LDD0449 
Eukaryotic translation initiation factor 2 subunit 3 (EIF2S3) LDTP03915 3.23  LDD0449 
Fructose-bisphosphate aldolase A (ALDOA) LDTP02008 2.64  LDD0453 
Glucose-6-phosphate isomerase (GPI) LDTP02162 10.00  LDD0449 
Glutamate dehydrogenase 1, mitochondrial (GLUD1) LDTP01768 10.00  LDD0449 
Glutathione S-transferase P (GSTP1) LDTP02333 10.00  LDD0453 
Heat shock cognate 71 kDa protein (HSPA8) LDTP02541 3.71  LDD0449 
Heat shock protein HSP 90-alpha (HSP90AA1) LDTP02225 1.60  LDD0449 
L-lactate dehydrogenase A chain (LDHA) LDTP01766 1.77  LDD0453 
L-lactate dehydrogenase B chain (LDHB) LDTP02179 1.76  LDD0449 
Myosin-7 (MYH7) LDTP02637 2.18  LDD0449 
Myosin-9 (MYH9) LDTP03764 2.52  LDD0449 
NEDD8-conjugating enzyme Ubc12 (UBE2M) LDTP04898 10.00  LDD0449 
Neutral alpha-glucosidase AB (GANAB) LDTP06192 2.42  LDD0453 
Peptidyl-prolyl cis-trans isomerase B (PPIB) LDTP03216 10.00  LDD0449 
Peroxiredoxin-1 (PRDX1) LDTP05503 1.87  LDD0449 
Phosphoglycerate kinase 1 (PGK1) LDTP01787 2.43  LDD0449 
Probable ATP-dependent RNA helicase DDX17 (DDX17) LDTP09899 10.00  LDD0449 
Probable ATP-dependent RNA helicase DDX46 (DDX46) LDTP07932 1.51  LDD0449 
Protein disulfide-isomerase A3 (PDIA3) LDTP03521 3.67  LDD0449 
Putative elongation factor 1-alpha-like 3 (EEF1A1P5) LDTP15029 1.52  LDD0449 
Pyruvate kinase PKM (PKM) LDTP02733 2.15  LDD0453 
Ras GTPase-activating protein-binding protein 1 (G3BP1) LDTP05812 2.68  LDD0449 
Retinaldehyde dehydrogenase 3 (ALDH1A3) LDTP04134 10.00  LDD0449 
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A) LDTP03522 10.00  LDD0449 
Thioredoxin (TXN) LDTP02502 10.00  LDD0449 
Triosephosphate isomerase (TPI1) LDTP04851 1.51  LDD0449 
Ubiquitin-like modifier-activating enzyme 1 (UBA1) LDTP03166 1.55  LDD0453 
UDP-glucose:glycoprotein glucosyltransferase 1 (UGGT1) LDTP12851 10.00  LDD0449 
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⏷ Show the Full List of 47 Enzyme
24 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
14-3-3 protein epsilon (YWHAE) LDTP04969 10.00  LDD0449 
14-3-3 protein gamma (YWHAG) LDTP04953 10.00  LDD0449 
14-3-3 protein zeta/delta (YWHAZ) LDTP05043 1.88  LDD0449 
ADP/ATP translocase 2 (SLC25A5) LDTP02087 1.98  LDD0449 
Amino acid transporter heavy chain SLC3A2 (SLC3A2) LDTP02256 2.17  LDD0449 
Annexin A1 (ANXA1) LDTP02010 5.66  LDD0449 
Annexin A2 (ANXA2) LDTP02199 3.26  LDD0449 
Annexin A5 (ANXA5) LDTP02307 10.00  LDD0449 
Astrotactin-1 (ASTN1) LDTP00362 10.00  LDD0453 
ATP synthase subunit alpha, mitochondrial (ATP5F1A) LDTP03327 2.40  LDD0449 
Clathrin heavy chain 1 (CLTC) LDTP05252 1.85  LDD0449 
Desmoplakin (DSP) LDTP02815 10.00  LDD0453 
Fatty acid-binding protein 5 (FABP5) LDTP05292 8.71  LDD0453 
Filamin-A (FLNA) LDTP03107 2.75  LDD0449 
Filamin-B (FLNB) LDTP01197 2.22  LDD0449 
Importin subunit beta-1 (KPNB1) LDTP06242 10.00  LDD0453 
Major vault protein (MVP) LDTP06203 10.00  LDD0449 
Protein S100-A10 (S100A10) LDTP04885 2.98  LDD0449 
RNA-binding protein with serine-rich domain 1 (RNPS1) LDTP06350 2.25  LDD0449 
Serine/arginine repetitive matrix protein 1 (SRRM1) LDTP08637 2.63  LDD0449 
Talin-1 (TLN1) LDTP14031 10.00  LDD0449 
Urea transporter 1 (SLC14A1) LDTP05832 10.00  LDD0453 
Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 2.24  LDD0453 
Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 2.08  LDD0449 
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⏷ Show the Full List of 24 Transporter and channel
3 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
High mobility group protein HMG-I/HMG-Y (HMGA1) LDTP02886 10.00  LDD0449 
Splicing factor, proline- and glutamine-rich (SFPQ) LDTP03212 10.00  LDD0449 
Transcription factor SOX-5 (SOX5) LDTP03781 10.00  LDD0449 
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1 GPCR Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Olfactory receptor 8H2 (OR8H2) LDTP17677 1.98  LDD0453 
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120 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
10 kDa heat shock protein, mitochondrial (HSPE1) LDTP04931 10.00  LDD0449 
14-3-3 protein beta/alpha (YWHAB) LDTP03617 3.30  LDD0449 
14-3-3 protein sigma (SFN) LDTP03618 8.09  LDD0449 
Adenylyl cyclase-associated protein 1 (CAP1) LDTP05294 10.00  LDD0449 
Adipogenesis regulatory factor (ADIRF) LDTP14866 10.00  LDD0449 
Alpha-actinin-1 (ACTN1) LDTP02631 10.00  LDD0449 
Alpha-actinin-4 (ACTN4) LDTP00843 1.50  LDD0453 
Amyloid beta precursor like protein 2 (APLP2) LDTP05491 2.37  LDD0449 
Arginine and glutamate-rich protein 1 (ARGLU1) LDTP16118 2.23  LDD0449 
Arginine/serine-rich coiled-coil protein 2 (RSRC2) LDTP07954 3.05  LDD0449 
Bcl-2-associated transcription factor 1 (BCLAF1) LDTP12826 4.20  LDD0449 
Calmodulin-like protein 5 (CALML5) LDTP12937 10.00  LDD0449 
Caprin-1 (CAPRIN1) LDTP06121 10.00  LDD0449 
Cilia- and flagella- associated protein 210 (CFAP210) LDTP19229 4.15  LDD0453 
Cofilin-1 (CFL1) LDTP03244 1.59  LDD0449 
Coiled-coil domain-containing protein 18 (CCDC18) LDTP17238 2.12  LDD0449 
Disheveled-associated activator of morphogenesis 1 (DAAM1) LDTP14045 2.29  LDD0453 
Elongation factor 1-beta (EEF1B2) LDTP03283 10.00  LDD0453 
Elongation factor 1-delta (EEF1D) LDTP03499 10.00  LDD0449 
Eukaryotic translation initiation factor 3 subunit D (EIF3D) LDTP00619 3.97  LDD0449 
Eukaryotic translation initiation factor 4H (EIF4H) LDTP06301 4.86  LDD0449 
Eukaryotic translation initiation factor 6 (EIF6) LDTP04743 10.00  LDD0449 
Ezrin (EZR) LDTP02779 1.93  LDD0449 
Fascin (FSCN1) LDTP06598 4.99  LDD0453 
Galectin-1 (LGALS1) LDTP02341 1.87  LDD0453 
Heat shock 70 kDa protein 1B (HSPA1B) LDTP02435 10.00  LDD0449 
Heat shock 70 kDa protein 6 (HSPA6) LDTP02884 1.88  LDD0449 
Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) LDTP02364 2.71  LDD0449 
Heterogeneous nuclear ribonucleoprotein F (HNRNPF) LDTP04514 2.15  LDD0449 
Heterogeneous nuclear ribonucleoprotein K (HNRNPK) LDTP04952 3.68  LDD0449 
Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP) LDTP00991 10.00  LDD0449 
Heterogeneous nuclear ribonucleoprotein R (HNRNPR) LDTP00756 10.00  LDD0453 
Heterogeneous nuclear ribonucleoprotein U (HNRNPU) LDTP05259 9.63  LDD0453 
Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC) LDTP02227 3.84  LDD0449 
Histone H1.2 (H1-2) LDTP02845 5.85  LDD0449 
Histone H1.5 (H1-5) LDTP02843 1.70  LDD0449 
Histone H2A type 1-J (H2AC14) LDTP10999 3.03  LDD0449 
Histone H3-7 (H3-7) LDTP15019 1.62  LDD0449 
Histone H4 (H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16) LDTP05004 2.87  LDD0453 
Interleukin enhancer-binding factor 3 (ILF3) LDTP05689 10.00  LDD0449 
Keratin, type I cuticular Ha3-II (KRT33B) LDTP06137 10.00  LDD0449 
Keratin, type I cytoskeletal 10 (KRT10) LDTP02674 2.23  LDD0453 
Keratin, type I cytoskeletal 13 (KRT13) LDTP02675 3.71  LDD0449 
Keratin, type I cytoskeletal 14 (KRT14) LDTP01930 1.72  LDD0453 
Keratin, type I cytoskeletal 15 (KRT15) LDTP02974 3.02  LDD0449 
Keratin, type I cytoskeletal 16 (KRT16) LDTP02308 1.60  LDD0453 
Keratin, type II cytoskeletal 1 (KRT1) LDTP02031 1.92  LDD0453 
Keratin, type II cytoskeletal 2 epidermal (KRT2) LDTP03792 1.85  LDD0453 
Keratin, type II cytoskeletal 4 (KRT4) LDTP02975 4.34  LDD0449 
Keratin, type II cytoskeletal 5 (KRT5) LDTP02676 1.84  LDD0453 
Keratin, type II cytoskeletal 6A (KRT6A) LDTP01931 1.88  LDD0453 
Keratin, type II cytoskeletal 8 (KRT8) LDTP02125 7.33  LDD0449 
KH domain-containing, RNA-binding, signal transduction-associated protein 1 (KHDRBS1) LDTP05525 10.00  LDD0449 
Laminin subunit beta-3 (LAMB3) LDTP05974 10.00  LDD0449 
Laminin subunit gamma-2 (LAMC2) LDTP05975 10.00  LDD0449 
Large ribosomal subunit protein eL14 (RPL14) LDTP04389 4.21  LDD0449 
Large ribosomal subunit protein eL18 (RPL18) LDTP05509 1.63  LDD0449 
Large ribosomal subunit protein eL30 (RPL30) LDTP05021 10.00  LDD0449 
Large ribosomal subunit protein eL31 (RPL31) LDTP05023 4.28  LDD0453 
Large ribosomal subunit protein eL8 (RPL7A) LDTP04990 1.83  LDD0449 
Large ribosomal subunit protein P2 (RPLP2) LDTP02108 10.00  LDD0449 
Large ribosomal subunit protein uL1 (RPL10A) LDTP05024 1.68  LDD0449 
Large ribosomal subunit protein uL10 (RPLP0) LDTP02109 10.00  LDD0449 
Large ribosomal subunit protein uL11 (RPL12) LDTP03517 2.14  LDD0449 
Large ribosomal subunit protein uL13 (RPL13A) LDTP03899 2.43  LDD0449 
Large ribosomal subunit protein uL16 (RPL10) LDTP03395 1.56  LDD0453 
Large ribosomal subunit protein uL18 (RPL5) LDTP04106 1.61  LDD0449 
Large ribosomal subunit protein uL2 (RPL8) LDTP05027 2.35  LDD0449 
Large ribosomal subunit protein uL22 (RPL17) LDTP02961 2.45  LDD0449 
Large ribosomal subunit protein uL23 (RPL23A) LDTP05001 6.12  LDD0449 
Large ribosomal subunit protein uL29 (RPL35) LDTP03999 1.51  LDD0453 
Large ribosomal subunit protein uL6 (RPL9; RPL9P7; RPL9P8; RPL9P9) LDTP03658 10.00  LDD0453 
LIM and SH3 domain protein 1 (LASP1) LDTP06220 2.65  LDD0449 
Matrin-3 (MATR3) LDTP04027 2.82  LDD0449 
Moesin (MSN) LDTP03346 10.00  LDD0453 
Myosin light polypeptide 6 (MYL6) LDTP04875 3.23  LDD0449 
Neuroblast differentiation-associated protein AHNAK (AHNAK) LDTP05597 3.02  LDD0449 
Nucleolin (NCL) LDTP02990 10.00  LDD0449 
Nucleophosmin (NPM1) LDTP02164 1.53  LDD0449 
Nucleosome assembly protein 1-like 1 (NAP1L1) LDTP04684 2.46  LDD0449 
PDZ and LIM domain protein 1 (PDLIM1) LDTP00193 10.00  LDD0449 
Plasminogen activator inhibitor 2 (SERPINB2) LDTP02084 2.70  LDD0449 
Plastin-2 (LCP1) LDTP02691 10.00  LDD0449 
Poly(rC)-binding protein 1 (PCBP1) LDTP06366 10.00  LDD0449 
Polyadenylate-binding protein 1 (PABPC1) LDTP02595 10.00  LDD0449 
Polypyrimidine tract-binding protein 1 (PTBP1) LDTP03364 1.72  LDD0449 
Pre-mRNA-splicing factor 38B (PRPF38B) LDTP17266 10.00  LDD0449 
Prelamin-A/C (LMNA) LDTP01932 10.00  LDD0449 
Protein S100-A11 (S100A11) LDTP03620 1.87  LDD0449 
Protein S100-A2 (S100A2) LDTP03463 2.51  LDD0449 
Protein S100-A7 (S100A7) LDTP03575 1.80  LDD0453 
Putative RNA-binding protein Luc7-like 1 (LUC7L) LDTP12388 3.14  LDD0453 
Rab GDP dissociation inhibitor beta (GDI2) LDTP04355 10.00  LDD0453 
Rab11 family-interacting protein 5 (RAB11FIP5) LDTP11374 2.86  LDD0449 
Ras-GEF domain-containing family member 1B (RASGEF1B) LDTP05609 1.64  LDD0453 
Regulator of G-protein signaling 22 (RGS22) LDTP09145 10.00  LDD0449 
Ryanodine receptor 3 (RYR3) LDTP06381 1.93  LDD0453 
Serine/arginine repetitive matrix protein 2 (SRRM2) LDTP13746 1.67  LDD0449 
Serine/arginine-rich splicing factor 2 (SRSF2) LDTP05280 1.51  LDD0449 
Small nuclear ribonucleoprotein Sm D2 (SNRPD2) LDTP04982 2.41  LDD0449 
Small ribosomal subunit protein eS19 (RPS19) LDTP03864 10.00  LDD0449 
Small ribosomal subunit protein eS21 (RPS21) LDTP05055 10.00  LDD0449 
Small ribosomal subunit protein eS26 (RPS26) LDTP05014 2.34  LDD0453 
Small ribosomal subunit protein eS27 (RPS27) LDTP03987 1.66  LDD0449 
Small ribosomal subunit protein eS6 (RPS6) LDTP05002 10.00  LDD0449 
Small ribosomal subunit protein eS8 (RPS8) LDTP04964 2.67  LDD0449 
Small ribosomal subunit protein uS11 (RPS14) LDTP04970 10.00  LDD0449 
Small ribosomal subunit protein uS13 (RPS18) LDTP04972 1.90  LDD0449 
Small ribosomal subunit protein uS15 (RPS13) LDTP04974 1.54  LDD0449 
Small ubiquitin-related modifier 3 (SUMO3) LDTP04717 2.10  LDD0449 
Splicing factor 3A subunit 2 (SF3A2) LDTP06387 2.03  LDD0449 
Src substrate cortactin (CTTN) LDTP06094 1.59  LDD0449 
T-complex protein 1 subunit alpha (TCP1) LDTP02934 10.00  LDD0449 
Testis-expressed protein 9 (TEX9) LDTP17734 2.89  LDD0453 
Transgelin-2 (TAGLN2) LDTP03846 1.62  LDD0449 
Tubulin beta-4B chain (TUBB4B) LDTP05076 10.00  LDD0449 
Tubulin-specific chaperone A (TBCA) LDTP01184 10.00  LDD0449 
Ubiquitin-ribosomal protein eS31 fusion protein (RPS27A) LDTP05033 2.21  LDD0449 
Uncharacterized protein C11orf98 (C11orf98) LDTP20164 10.00  LDD0453 
Vimentin (VIM) LDTP02297 3.03  LDD0453 
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⏷ Show the Full List of 120 Other

Full Information of The Labelling Profiles of This Probe

Quantification: Probe vs DMSO
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
1 uM
Quantitative Method
LFQ
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Skin
Disease Model
Skin squamous cell carcinoma [ICD-11:2C31]
Model Name
Human skin squamous cell carcinoma cells (A431)
Interaction Atlas ID
 LDD0449 
Download The Altas
  
Experiment 2 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
1 uM
Quantitative Method
LFQ
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Cervix uteri
Disease Model
Endocervical adenocarcinoma [ICD-11:2C77]
Model Name
Human endocervical adenocarcinoma cells (HeLa)
Interaction Atlas ID
 LDD0453 
Download The Altas

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.