Details of the Target

General Information of Target

Target ID LDTP08190
Target Name E3 ubiquitin-protein ligase RNF180 (RNF180)
Gene Name RNF180
Gene ID 285671
Synonyms
E3 ubiquitin-protein ligase RNF180; EC 2.3.2.27; RING finger protein 180; RING-type E3 ubiquitin transferase RNF180
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKRSKELITKNHSQEETSILRCWKCRKCIASSGCFMEYLENQVIKDKDDSVDAQNICHVW
HMNVEALPEWISCLIQKAQWTVGKLNCPFCGARLGGFNFVSTPKCSCGQLAAVHLSKSRT
DYQPTQAGRLMRPSVKYLSHPRVQSGCDKEALLTGGGSENRNHRLLNMARNNNDPGRLTE
ALCLEVRPTYFEMKNEKLLSKASEPKYQLFVPQLVTGRCATRAFHRKSHSLDLNISEKLT
LLPTLYEIHSKTTAYSRLNETQPIDLSGLPLQSSKNSYSFQNPSSFDPSMLLQRFSVAPH
ETQTQRGGEFQCGLEAASVYSDHTNTNNLTFLMDLPSAGRSMPEASDQEEHLSPLDFLHS
ANFSLGSINQRLNKRERSKLKNLRRKQRRRERWLQKQGKYSGVGLLDHMTLNNEMSTDED
NEYAEEKDSYICAVCLDVYFNPYMCYPCHHIFCEPCLRTLAKDNPSSTPCPLCRTIISRV
FFQTELNNATKTFFTKEYLKIKQSFQKSNSAKWPLPSCRKAFHLFGGFRRHAAPVTRRQF
PHGAHRMDYLHFEDDSRGWWFDMDMVIIYIYSVNWVIGFIVFCFLCYFFFPF
Target Bioclass
Enzyme
Subcellular location
Endoplasmic reticulum membrane
Function E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2.
Uniprot ID
Q86T96
Ensemble ID
ENST00000296615.10
HGNC ID
HGNC:27752

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCLP1 SNV: p.R474W .
HGC27 SNV: p.H140L .
HT115 SNV: p.R170L; p.E309K .
HUH28 SNV: p.E69Q .
KURAMOCHI SNV: p.D265Y .
LNCaP clone FGC SNV: p.A30T .
MEWO SNV: p.L524F .
MM1S SNV: p.V43L .
NCIH2172 SNV: p.H359L .
OCIAML2 SNV: p.D287N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P1
 Probe Info  		5.26  LDD0452  [1]
P2
 Probe Info  		4.82  LDD0453  [1]
P3
 Probe Info  		7.39  LDD0454  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060