Probe ID	LDPC0249
Probe Name	P3
Synonyms	2249695-94-9; EN300-26575790; (E)-4-(Dimethylamino)-N-(4-prop-2-ynoxyphenyl)but-2-enamide; 4-(dimethylamino)-N-[4-(prop-2-yn-1-yloxy)phenyl]but-2-enamide
Probe Type	ABPP Probe
Structure
	3D MOL			2D MOL
Ro5 Violations (Lipinski): 0	Molecular Weight (mw)		258.32
	Lipid-water partition coefficient (xlogp)		1.5
	Rotatable Bond Count (rotbonds)		6
	Hydrogen Bond Donor Count (hbonddonor)		1
	Hydrogen Bond Acceptor Count (hbondacc)		3
Chemical Identifiers	Formula C15H18N2O2 IUPAC Name (E)-4-(dimethylamino)-N-(4-prop-2-ynoxyphenyl)but-2-enamide Canonical SMILES CN(C)CC=CC(=O)NC1=CC=C(C=C1)OCC#C InChI InChI=1S/C15H18N2O2/c1-4-12-19-14-9-7-13(8-10-14)16-15(18)6-5-11-17(2)3/h1,5-10H,11-12H2,2-3H3,(H,16,18)/b6-5+ InChIKey PIDAROUISLSFJF-AATRIKPKSA-N

59 Enzyme Labeled by This Probe Target Name Target ID Binding Site(Ratio) Interaction ID 60 kDa heat shock protein, mitochondrial (HSPD1) LDTP02522 3.01  LDD0450  Actin, cytoplasmic 1 (ACTB) LDTP04876 8.38  LDD0454  Actin, cytoplasmic 2 (ACTG1) LDTP05059 1.50  LDD0450  Adenosylhomocysteinase (AHCY) LDTP03242 2.45  LDD0450  ATP-dependent RNA helicase A (DHX9) LDTP05550 10.00  LDD0450  Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase (PAICS) LDTP03159 10.00  LDD0450  Calpain-2 catalytic subunit (CAPN2) LDTP02911 10.00  LDD0450  Cardiomyopathy-associated protein 5 (CMYA5) LDTP08837 10.00  LDD0450  Catalase (CAT) LDTP02001 7.19  LDD0450  Cell division control protein 42 homolog (CDC42) LDTP04886 10.00  LDD0454  Creatine kinase B-type (CKB) LDTP02616 3.21  LDD0454  Cytochrome b5 type B (CYB5B) LDTP00697 10.00  LDD0450  D-3-phosphoglycerate dehydrogenase (PHGDH) LDTP00701 10.00  LDD0450  Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial (DUT) LDTP03669 2.49  LDD0450  Dermcidin (DCD) LDTP05171 1.54  LDD0454  E3 ubiquitin-protein ligase RNF180 (RNF180) LDTP08190 7.39  LDD0454  EH domain-containing protein 3 (EHD3) LDTP12922 2.48  LDD0450  Elongation factor 1-alpha 2 (EEF1A2) LDTP05460 3.21  LDD0454  Elongation factor 2 (EEF2) LDTP02672 2.11  LDD0450  Endoplasmic reticulum chaperone BiP (HSPA5) LDTP02534 10.00  LDD0454  Epidermal growth factor receptor (EGFR) LDTP01785 8.57  LDD0450  Eukaryotic initiation factor 4A-I (EIF4A1) LDTP04878 1.84  LDD0450  Eukaryotic translation initiation factor 2 subunit 3 (EIF2S3) LDTP03915 3.42  LDD0450  Fructose-bisphosphate aldolase A (ALDOA) LDTP02008 1.58  LDD0450  GDP-L-fucose synthase (GFUS) LDTP05954 10.00  LDD0450  Glucose-6-phosphate isomerase (GPI) LDTP02162 5.18  LDD0450  Heat shock cognate 71 kDa protein (HSPA8) LDTP02541 3.58  LDD0450  Heat shock protein HSP 90-alpha (HSP90AA1) LDTP02225 1.60  LDD0450  L-lactate dehydrogenase A chain (LDHA) LDTP01766 1.99  LDD0454  L-lactate dehydrogenase B chain (LDHB) LDTP02179 1.56  LDD0450  Lysine--tRNA ligase (KARS1) LDTP06294 10.00  LDD0450  Macrophage migration inhibitory factor (MIF) LDTP02713 10.00  LDD0450  Malate dehydrogenase, mitochondrial (MDH2) LDTP03908 4.68  LDD0450  Myosin-9 (MYH9) LDTP03764 1.94  LDD0450  NEDD8-conjugating enzyme Ubc12 (UBE2M) LDTP04898 10.00  LDD0450  Neutral alpha-glucosidase AB (GANAB) LDTP06192 2.04  LDD0450  Nucleolar RNA helicase 2 (DDX21) LDTP12469 3.23  LDD0450  Nucleoside diphosphate kinase A (NME1) LDTP02796 1.67  LDD0450  Peptidyl-prolyl cis-trans isomerase B (PPIB) LDTP03216 10.00  LDD0450  Peroxiredoxin-1 (PRDX1) LDTP05503 1.72  LDD0450  Phosphoglycerate kinase 1 (PGK1) LDTP01787 2.14  LDD0450  Phosphoglycerate mutase 2 (PGAM2) LDTP02774 1.54  LDD0454  Probable ATP-dependent RNA helicase DDX46 (DDX46) LDTP07932 1.52  LDD0450  Probable phospholipid-transporting ATPase IIA (ATP9A) LDTP01124 2.28  LDD0454  Protein disulfide-isomerase (P4HB) LDTP02188 10.00  LDD0450  Protein disulfide-isomerase A3 (PDIA3) LDTP03521 1.75  LDD0450  Pyruvate kinase PKM (PKM) LDTP02733 1.66  LDD0454  Ras GTPase-activating protein-binding protein 1 (G3BP1) LDTP05812 3.49  LDD0450  Ras-related protein Rab-7a (RAB7A) LDTP04399 5.22  LDD0450  Serine/threonine-protein kinase PRP4 homolog (PRPF4B) LDTP05911 1.63  LDD0454  Thioredoxin (TXN) LDTP02502 10.00  LDD0450  Transaldolase (TALDO1) LDTP03847 1.77  LDD0450  Transitional endoplasmic reticulum ATPase (VCP) LDTP04665 10.00  LDD0450  Transketolase (TKT) LDTP03487 1.70  LDD0450  Triosephosphate isomerase (TPI1) LDTP04851 1.61  LDD0454  Tubulin--tyrosine ligase-like protein 12 (TTLL12) LDTP06067 10.00  LDD0450  Ubiquitin-like modifier-activating enzyme 1 (UBA1) LDTP03166 10.00  LDD0450  UDP-glucose:glycoprotein glucosyltransferase 1 (UGGT1) LDTP12851 10.00  LDD0450  Very-long-chain enoyl-CoA reductase (TECR) LDTP12867 3.42  LDD0450  ------------------------------------------------------------------------------------ ⏷ Show the Full List of 59 Enzyme
27 Transporter and channel Labeled by This Probe Target Name Target ID Binding Site(Ratio) Interaction ID 14-3-3 protein theta (YWHAQ) LDTP03385 10.00  LDD0450  14-3-3 protein zeta/delta (YWHAZ) LDTP05043 1.53  LDD0450  ADP/ATP translocase 2 (SLC25A5) LDTP02087 1.77  LDD0450  Amino acid transporter heavy chain SLC3A2 (SLC3A2) LDTP02256 1.56  LDD0450  Annexin A1 (ANXA1) LDTP02010 5.39  LDD0450  Annexin A2 (ANXA2) LDTP02199 4.35  LDD0450  Annexin A5 (ANXA5) LDTP02307 10.00  LDD0450  Astrotactin-1 (ASTN1) LDTP00362 10.00  LDD0454  Chloride intracellular channel protein 1 (CLIC1) LDTP00244 7.91  LDD0454  Clathrin heavy chain 1 (CLTC) LDTP05252 1.77  LDD0450  Cystatin-B (CSTB) LDTP02009 10.00  LDD0450  Desmoplakin (DSP) LDTP02815 7.72  LDD0454  Fatty acid-binding protein 5 (FABP5) LDTP05292 10.00  LDD0450  Filamin-A (FLNA) LDTP03107 1.88  LDD0450  Filamin-B (FLNB) LDTP01197 4.31  LDD0450  Importin subunit alpha-3 (KPNA4) LDTP00328 10.00  LDD0450  Importin subunit beta-1 (KPNB1) LDTP06242 10.00  LDD0454  Protein S100-A10 (S100A10) LDTP04885 3.34  LDD0450  Protein SON (SON) LDTP02959 1.71  LDD0450  RNA-binding protein with serine-rich domain 1 (RNPS1) LDTP06350 2.30  LDD0450  Serine/arginine repetitive matrix protein 1 (SRRM1) LDTP08637 1.78  LDD0450  Solute carrier family 25 member 44 (SLC25A44) LDTP15733 2.09  LDD0450  T-complex protein 1 subunit delta (CCT4) LDTP04391 10.00  LDD0450  Talin-1 (TLN1) LDTP14031 10.00  LDD0450  Urea transporter 1 (SLC14A1) LDTP05832 10.00  LDD0454  Voltage-dependent anion-selective channel protein 1 (VDAC1) LDTP03132 2.62  LDD0454  Voltage-dependent anion-selective channel protein 2 (VDAC2) LDTP04071 10.00  LDD0450  ------------------------------------------------------------------------------------ ⏷ Show the Full List of 27 Transporter and channel
2 Transcription factor Labeled by This Probe Target Name Target ID Binding Site(Ratio) Interaction ID Non-POU domain-containing octamer-binding protein (NONO) LDTP06341 5.80  LDD0450  Zinc finger protein 99 (ZNF99) LDTP16710 10.00  LDD0454  ------------------------------------------------------------------------------------
1 GPCR Labeled by This Probe Target Name Target ID Binding Site(Ratio) Interaction ID Olfactory receptor 8H2 (OR8H2) LDTP17677 2.17  LDD0454  ------------------------------------------------------------------------------------
112 Other Labeled by This Probe Target Name Target ID Binding Site(Ratio) Interaction ID 14-3-3 protein beta/alpha (YWHAB) LDTP03617 3.13  LDD0450  14-3-3 protein sigma (SFN) LDTP03618 10.00  LDD0450  Alpha-actinin-1 (ACTN1) LDTP02631 10.00  LDD0450  Alpha-actinin-4 (ACTN4) LDTP00843 1.64  LDD0450  Arginine/serine-rich coiled-coil protein 2 (RSRC2) LDTP07954 5.18  LDD0450  Bcl-2-associated transcription factor 1 (BCLAF1) LDTP12826 4.01  LDD0450  Caprin-1 (CAPRIN1) LDTP06121 10.00  LDD0450  Centriolin (CNTRL) LDTP08139 9.49  LDD0454  Cilia- and flagella- associated protein 210 (CFAP210) LDTP19229 2.26  LDD0454  Cofilin-1 (CFL1) LDTP03244 1.85  LDD0450  Disheveled-associated activator of morphogenesis 1 (DAAM1) LDTP14045 1.68  LDD0454  Elongation factor 1-delta (EEF1D) LDTP03499 10.00  LDD0450  Elongation factor 1-gamma (EEF1G) LDTP03367 1.55  LDD0450  Eukaryotic translation initiation factor 3 subunit D (EIF3D) LDTP00619 4.71  LDD0450  Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) LDTP05421 10.00  LDD0450  Eukaryotic translation initiation factor 4H (EIF4H) LDTP06301 2.82  LDD0450  Eukaryotic translation initiation factor 6 (EIF6) LDTP04743 10.00  LDD0450  Ezrin (EZR) LDTP02779 1.66  LDD0450  Fascin (FSCN1) LDTP06598 1.55  LDD0450  Galectin-1 (LGALS1) LDTP02341 2.43  LDD0454  Heat shock 70 kDa protein 1B (HSPA1B) LDTP02435 10.00  LDD0450  Heat shock 70 kDa protein 6 (HSPA6) LDTP02884 1.71  LDD0450  Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1) LDTP02364 1.94  LDD0450  Heterogeneous nuclear ribonucleoprotein A3 (HNRNPA3) LDTP04495 10.00  LDD0450  Heterogeneous nuclear ribonucleoprotein F (HNRNPF) LDTP04514 4.29  LDD0450  Heterogeneous nuclear ribonucleoprotein H (HNRNPH1) LDTP03615 10.00  LDD0450  Heterogeneous nuclear ribonucleoprotein K (HNRNPK) LDTP04952 1.86  LDD0450  Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP) LDTP00991 10.00  LDD0450  Heterogeneous nuclear ribonucleoprotein R (HNRNPR) LDTP00756 10.00  LDD0454  Heterogeneous nuclear ribonucleoprotein U (HNRNPU) LDTP05259 10.00  LDD0454  Heterogeneous nuclear ribonucleoproteins A2/B1 (HNRNPA2B1) LDTP03182 1.90  LDD0450  Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC) LDTP02227 3.62  LDD0450  Histone H1.2 (H1-2) LDTP02845 2.08  LDD0450  Histone H4 (H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16) LDTP05004 1.67  LDD0454  Junction plakoglobin (JUP) LDTP02756 2.29  LDD0450  Keratin, type I cytoskeletal 13 (KRT13) LDTP02675 10.00  LDD0450  Keratin, type I cytoskeletal 14 (KRT14) LDTP01930 3.15  LDD0450  Keratin, type I cytoskeletal 15 (KRT15) LDTP02974 10.00  LDD0450  Keratin, type I cytoskeletal 17 (KRT17) LDTP05424 5.81  LDD0450  Keratin, type II cytoskeletal 4 (KRT4) LDTP02975 10.00  LDD0450  Keratin, type II cytoskeletal 8 (KRT8) LDTP02125 10.00  LDD0454  KH domain-containing, RNA-binding, signal transduction-associated protein 1 (KHDRBS1) LDTP05525 10.00  LDD0450  Laminin subunit beta-3 (LAMB3) LDTP05974 10.00  LDD0450  Large ribosomal subunit protein eL14 (RPL14) LDTP04389 2.93  LDD0450  Large ribosomal subunit protein eL15 (RPL15) LDTP04919 2.62  LDD0450  Large ribosomal subunit protein eL18 (RPL18) LDTP05509 2.96  LDD0450  Large ribosomal subunit protein eL19 (RPL19) LDTP05196 1.67  LDD0450  Large ribosomal subunit protein eL29 (RPL29) LDTP04140 1.64  LDD0450  Large ribosomal subunit protein eL30 (RPL30) LDTP05021 10.00  LDD0450  Large ribosomal subunit protein eL31 (RPL31) LDTP05023 1.79  LDD0454  Large ribosomal subunit protein eL34 (RPL34) LDTP04233 1.80  LDD0450  Large ribosomal subunit protein eL6 (RPL6) LDTP05378 1.69  LDD0450  Large ribosomal subunit protein eL8 (RPL7A) LDTP04990 1.68  LDD0450  Large ribosomal subunit protein uL1 (RPL10A) LDTP05024 2.46  LDD0450  Large ribosomal subunit protein uL10 (RPLP0) LDTP02109 10.00  LDD0450  Large ribosomal subunit protein uL11 (RPL12) LDTP03517 2.33  LDD0450  Large ribosomal subunit protein uL13 (RPL13A) LDTP03899 6.56  LDD0450  Large ribosomal subunit protein uL16 (RPL10) LDTP03395 1.84  LDD0450  Large ribosomal subunit protein uL18 (RPL5) LDTP04106 1.83  LDD0454  Large ribosomal subunit protein uL2 (RPL8) LDTP05027 1.74  LDD0450  Large ribosomal subunit protein uL22 (RPL17) LDTP02961 2.33  LDD0450  Large ribosomal subunit protein uL23 (RPL23A) LDTP05001 4.04  LDD0450  Large ribosomal subunit protein uL4 (RPL4) LDTP03815 1.51  LDD0450  LIM and SH3 domain protein 1 (LASP1) LDTP06220 1.87  LDD0450  Luc7-like protein 3 (LUC7L3) LDTP01534 5.68  LDD0454  Matrin-3 (MATR3) LDTP04027 3.67  LDD0450  Melanoma-associated antigen 4 (MAGEA4) LDTP04041 10.00  LDD0450  Moesin (MSN) LDTP03346 10.00  LDD0454  Myosin light polypeptide 6 (MYL6) LDTP04875 2.82  LDD0450  Neuroblast differentiation-associated protein AHNAK (AHNAK) LDTP05597 2.59  LDD0450  Nucleolin (NCL) LDTP02990 7.22  LDD0450  Nucleosome assembly protein 1-like 1 (NAP1L1) LDTP04684 1.54  LDD0450  PDZ and LIM domain protein 1 (PDLIM1) LDTP00193 10.00  LDD0450  Perilipin-5 (PLIN5) LDTP05269 4.60  LDD0450  Plasminogen activator inhibitor 2 (SERPINB2) LDTP02084 4.04  LDD0450  Plastin-2 (LCP1) LDTP02691 10.00  LDD0450  Poly(rC)-binding protein 1 (PCBP1) LDTP06366 10.00  LDD0450  Polyadenylate-binding protein 1 (PABPC1) LDTP02595 10.00  LDD0450  Polypyrimidine tract-binding protein 1 (PTBP1) LDTP03364 2.12  LDD0450  Pre-mRNA-splicing factor 38B (PRPF38B) LDTP17266 10.00  LDD0450  Prelamin-A/C (LMNA) LDTP01932 5.79  LDD0450  Proteasome activator complex subunit 1 (PSME1) LDTP05484 10.00  LDD0450  Protein S100-A11 (S100A11) LDTP03620 4.06  LDD0450  Protein S100-A14 (S100A14) LDTP12277 10.00  LDD0450  Protein S100-A2 (S100A2) LDTP03463 10.00  LDD0450  Protein S100-P (S100P) LDTP03336 10.00  LDD0450  Putative HLA class I histocompatibility antigen, alpha chain H (HLA-H) LDTP01914 10.00  LDD0450  Rab GDP dissociation inhibitor beta (GDI2) LDTP04355 8.89  LDD0454  Reticulon-3 (RTN3) LDTP01522 10.00  LDD0450  Reticulon-4 (RTN4) LDTP12412 10.00  LDD0450  Serine/arginine repetitive matrix protein 2 (SRRM2) LDTP13746 1.60  LDD0450  Serine/arginine-rich splicing factor 6 (SRSF6) LDTP05802 1.94  LDD0450  Serpin B11 (SERPINB11) LDTP10650 10.00  LDD0454  Small nuclear ribonucleoprotein Sm D2 (SNRPD2) LDTP04982 2.00  LDD0450  Small ribosomal subunit protein eS19 (RPS19) LDTP03864 10.00  LDD0450  Small ribosomal subunit protein eS26 (RPS26) LDTP05014 10.00  LDD0450  Small ribosomal subunit protein eS4, X isoform (RPS4X) LDTP04997 1.85  LDD0450  Small ribosomal subunit protein eS8 (RPS8) LDTP04964 3.64  LDD0450  Small ribosomal subunit protein uS11 (RPS14) LDTP04970 10.00  LDD0450  Small ribosomal subunit protein uS15 (RPS13) LDTP04974 1.51  LDD0454  Small ubiquitin-related modifier 3 (SUMO3) LDTP04717 1.82  LDD0450  Splicing factor U2AF 26 kDa subunit (U2AF1L4) LDTP09562 1.76  LDD0454  Src substrate cortactin (CTTN) LDTP06094 4.38  LDD0450  Thyroid hormone receptor-associated protein 3 (THRAP3) LDTP13919 1.77  LDD0450  Transgelin-2 (TAGLN2) LDTP03846 2.82  LDD0450  Translationally-controlled tumor protein (TPT1) LDTP02683 10.00  LDD0450  Tropomyosin alpha-3 chain (TPM3) LDTP02165 10.00  LDD0450  Tubulin beta-4B chain (TUBB4B) LDTP05076 10.00  LDD0450  Tubulin-specific chaperone A (TBCA) LDTP01184 10.00  LDD0450  Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2) LDTP17377 10.00  LDD0450  Ubiquitin-ribosomal protein eS31 fusion protein (RPS27A) LDTP05033 1.95  LDD0450  Vimentin (VIM) LDTP02297 6.28  LDD0454  ------------------------------------------------------------------------------------ ⏷ Show the Full List of 112 Other

Competitor ID	Name	Concentration		Cell-system	Ref
LDCM0166	Afatinib	.		Human skin squamous cell carcinoma cells (A431)	[1]

1	Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.