Details of the Target

General Information of Target

Target ID LDTP04041
Target Name Melanoma-associated antigen 4 (MAGEA4)
Gene Name MAGEA4
Gene ID 4103
Synonyms
MAGE4; Melanoma-associated antigen 4; Cancer/testis antigen 1.4; CT1.4; MAGE-4 antigen; MAGE-41 antigen; MAGE-X2 antigen
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESA
GPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHF
LLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPASNTYTLV
TCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVY
GEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRI
AYPSLREAALLEEEEGV
Target Type
Clinical trial
Target Bioclass
Other
Function Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase. Also negatively regulates p53-mediated apoptosis.
TTD ID
T12097
Uniprot ID
P43358
DrugMap ID
TT9EQUY
Ensemble ID
ENST00000276344.6
HGNC ID
HGNC:6802
ChEMBL ID
CHEMBL4296022

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
P3
 Probe Info  		10.00  LDD0450  [2]
P8
 Probe Info  		10.00  LDD0451  [2]
DBIA
 Probe Info  		C147(2.77)  LDD3331  [3]
EA-probe
 Probe Info  		N.A.  LDD0440  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [5]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [6]
PF-06672131
 Probe Info  		N.A.  LDD0017  [7]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DR-1
 Probe Info  		2.99  LDD0398  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 12.50  LDD0403  [1]
 LDCM0175  Ethacrynic acid HeLa N.A.  LDD0440  [4]
 LDCM0022  KB02 22RV1 C147(2.09)  LDD2243  [3]
 LDCM0023  KB03 22RV1 C147(3.36)  LDD2660  [3]
 LDCM0024  KB05 MKN-1 C147(2.77)  LDD3331  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pseudouridine-5'-phosphatase (PUDP) CbbY/CbbZ/Gph/YieH family Q08623
E3 ubiquitin-protein ligase RAD18 (RAD18) RAD18 family Q9NS91
Thioredoxin, mitochondrial (TXN2) Thioredoxin family Q99757
E3 ubiquitin-protein ligase TRIM69 (TRIM69) TRIM/RBCC family Q86WT6
Cytochrome b-c1 complex subunit 7 (UQCRB) UQCRB/QCR7 family P14927
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytochrome c oxidase subunit 8A, mitochondrial (COX8A) Cytochrome c oxidase VIII family P10176
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger and BTB domain-containing protein 17 (ZBTB17) Krueppel C2H2-type zinc-finger protein family Q13105
Zinc finger protein 576 (ZNF576) Krueppel C2H2-type zinc-finger protein family Q9H609
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Testis-specific Y-encoded-like protein 4 (TSPYL4) Nucleosome assembly protein (NAP) family Q9UJ04
Tektin-4 (TEKT4) Tektin family Q8WW24
Protection of telomeres protein 1 (POT1) Telombin family Q9NUX5
UV-stimulated scaffold protein A (UVSSA) UVSSA family Q2YD98
26S proteasome non-ATPase regulatory subunit 10 (PSMD10) . O75832
Low density lipoprotein receptor adapter protein 1 (LDLRAP1) . Q5SW96
PRKCA-binding protein (PICK1) . Q9NRD5
UBX domain-containing protein 6 (UBXN6) . Q9BZV1

The Drug(s) Related To This Target

Phase 2
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Car-t Cells Targeting Mage-a4 CAR T Cell Therapy D0XZ2S
Adp-a2m4 . D2YT5I

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Chemoproteomic Profiling Reveals Ethacrynic Acid Targets Adenine Nucleotide Translocases to Impair Mitochondrial Function. Mol Pharm. 2018 Jun 4;15(6):2413-2422. doi: 10.1021/acs.molpharmaceut.8b00250. Epub 2018 May 15.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 Site-Specific Activity-Based Protein Profiling Using Phosphonate Handles. Mol Cell Proteomics. 2023 Jan;22(1):100455. doi: 10.1016/j.mcpro.2022.100455. Epub 2022 Nov 24.
Mass spectrometry data entry: PXD036569
8 Quantitative Proteomics Reveals Cellular Off-Targets of a DDR1 Inhibitor. ACS Med Chem Lett. 2020 Feb 5;11(4):535-540. doi: 10.1021/acsmedchemlett.9b00658. eCollection 2020 Apr 9.