Details of the Target

General Information of Target

Target ID LDTP04717
Target Name Small ubiquitin-related modifier 3 (SUMO3)
Gene Name SUMO3
Gene ID 6612
Synonyms
SMT3A; SMT3H1; Small ubiquitin-related modifier 3; SUMO-3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3A; Smt3A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFR
FDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVPESSLAGHSF
Target Bioclass
Other
Family
Ubiquitin family, SUMO subfamily
Subcellular location
Cytoplasm
Function
Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Plays a role in the regulation of sumoylation status of SETX.
Uniprot ID
P55854
Ensemble ID
ENST00000332859.11
HGNC ID
HGNC:11124
ChEMBL ID
CHEMBL3885639

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
JURKAT SNV: p.C47Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P2
 Probe Info  		2.10  LDD0449  [1]
P3
 Probe Info  		1.82  LDD0450  [1]
ONAyne
 Probe Info  		K32(1.02)  LDD0274  [2]
STPyne
 Probe Info  		K32(4.46); K41(5.88); K44(5.26)  LDD0277  [2]
Acrolein
 Probe Info  		N.A.  LDD0221  [3]
AMP probe
 Probe Info  		K32(0.00); K34(0.00)  LDD0200  [4]
ATP probe
 Probe Info  		K32(0.00); K34(0.00); K41(0.00); K11(0.00)  LDD0199  [4]
ATP probe
 Probe Info  		K32(0.00); K41(0.00)  LDD0035  [5]
HHS-465
 Probe Info  		K32(0.00); K44(0.00); Y46(0.00); K41(0.00)  LDD2240  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [3]
 LDCM0107  IAA HeLa N.A.  LDD0221  [3]
 LDCM0109  NEM HeLa N.A.  LDD0223  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SUMO-specific isopeptidase USPL1 (USPL1) Peptidase C19 family Q5W0Q7
Sentrin-specific protease 2 (SENP2) Peptidase C48 family Q9HC62
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Transcription factor
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger and BTB domain-containing protein 39 (ZBTB39) Krueppel C2H2-type zinc-finger protein family O15060
Zinc finger protein 496 (ZNF496) Krueppel C2H2-type zinc-finger protein family Q96IT1
Nuclear autoantigen Sp-100 (SP100) . P23497
Transcriptional regulator Kaiso (ZBTB33) . Q86T24
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
Protein FAM221A (FAM221A) FAM221 family A4D161
U4/U6.U5 tri-snRNP-associated protein 1 (SART1) SNU66/SART1 family O43290
Zinc finger CCHC domain-containing protein 12 (ZCCHC12) ZCCHC12 family Q6PEW1

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
5 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
6 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010