Details of the Target

General Information of Target

Target ID LDTP09562
Target Name Splicing factor U2AF 26 kDa subunit (U2AF1L4)
Gene Name U2AF1L4
Gene ID 199746
Synonyms
U2AF1-RS3; U2AF1L3; Splicing factor U2AF 26 kDa subunit; U2 auxiliary factor 26; U2 small nuclear RNA auxiliary factor 1-like protein 4; U2AF1-like 4; U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3; U2 small nuclear RNA auxiliary factor 1-like protein 3; U2AF1-like protein 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEYLASIFGTEKDKVNCSFYFKIGVCRHGDRCSRLHNKPTFSQTIVLLNLYRNPQNTAQ
TADGSHCHVSDVEVQEHYDSFFEEVFTELQEKYGEIEEMNVCDNLGDHLVGNVYVKFRRE
EDGERAVAELSNRWFNGQAVHGELSPVTDFRESCCRQYEMGECTRGGFCNFMHLRPISQN
LQRQLYGRGPRRRSPPRFHTGHHPRERNHRCSPDHWHGRF
Target Bioclass
Other
Family
Splicing factor SR family
Subcellular location
Nucleus
Function
RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T-cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site. Shows a preference for AGC or AGA.
Uniprot ID
Q8WU68
Ensemble ID
ENST00000292879.9
HGNC ID
HGNC:23020

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P1
 Probe Info  		2.79  LDD0452  [1]
P3
 Probe Info  		1.76  LDD0454  [1]
P8
 Probe Info  		6.26  LDD0455  [1]
YN-4
 Probe Info  		100.00  LDD0445  [2]
DBIA
 Probe Info  		C90(1.21)  LDD3310  [3]
IA-alkyne
 Probe Info  		C163(10.21)  LDD0372  [4]
SF
 Probe Info  		N.A.  LDD0028  [5]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA HEK-293T C163(10.21)  LDD0372  [4]
 LDCM0022  KB02 22RV1 C90(1.19); C18(1.48)  LDD2243  [3]
 LDCM0023  KB03 22RV1 C90(1.40); C18(2.00)  LDD2660  [3]
 LDCM0024  KB05 COLO792 C90(1.21)  LDD3310  [3]

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
6 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.