General Information of Target

Target ID LDTP06350
Target Name RNA-binding protein with serine-rich domain 1 (RNPS1)
Gene Name RNPS1
Gene ID 10921
Synonyms
LDC2; RNA-binding protein with serine-rich domain 1; SR-related protein LDC2
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MDLSGVKKKSLLGVKENNKKSSTRAPSPTKRKDRSDEKSKDRSKDKGATKESSEKDRGRD
KTRKRRSASSGSSSTRSRSSSTSSSGSSTSTGSSSGSSSSSASSRSGSSSTSRSSSSSSS
SGSPSPSRRRHDNRRRSRSKSKPPKRDEKERKRRSPSPKPTKVHIGRLTRNVTKDHIMEI
FSTYGKIKMIDMPVERMHPHLSKGYAYVEFENPDEAEKALKHMDGGQIDGQEITATAVLA
PWPRPPPRRFSPPRRMLPPPPMWRRSPPRMRRRSRSPRRRSPVRRRSRSPGRRRHRSRSS
SNSSR
Target Bioclass
Transporter and channel
Family
Splicing factor SR family
Subcellular location
Nucleus
Function
Part of pre- and post-splicing multiprotein mRNP complexes. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Enhances the formation of the ATP-dependent A complex of the spliceosome. Involved in both constitutive splicing and, in association with SRP54 and TRA2B/SFRS10, in distinctive modulation of alternative splicing in a substrate-dependent manner. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Participates in mRNA 3'-end cleavage. Involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Also mediates increase of mRNA abundance and translational efficiency. Binds spliced mRNA 20-25 nt upstream of exon-exon junctions.
Uniprot ID
Q15287
Ensemble ID
ENST00000301730.12
HGNC ID
HGNC:10080

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL72 SNV: p.A206G .
PC9 SNV: p.M189I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 15 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P1
 Probe Info 
2.74  LDD0448  [1]
P2
 Probe Info 
2.25  LDD0449  [1]
P3
 Probe Info 
2.30  LDD0450  [1]
P8
 Probe Info 
3.33  LDD0451  [1]
C-Sul
 Probe Info 
6.85  LDD0066  [2]
ONAyne
 Probe Info 
N.A.  LDD0273  [3]
HHS-475
 Probe Info 
Y207(1.74)  LDD0264  [4]
Acrolein
 Probe Info 
N.A.  LDD0221  [5]
ATP probe
 Probe Info 
K218(0.00); K221(0.00); K203(0.00); K174(0.00)  LDD0199  [6]
1d-yne
 Probe Info 
N.A.  LDD0358  [7]
NHS
 Probe Info 
N.A.  LDD0010  [8]
SF
 Probe Info 
Y207(0.00); Y205(0.00)  LDD0028  [9]
STPyne
 Probe Info 
N.A.  LDD0009  [8]
1c-yne
 Probe Info 
K188(0.00); K186(0.00); K174(0.00)  LDD0228  [7]
HHS-465
 Probe Info 
N.A.  LDD2240  [10]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C364
 Probe Info 
15.14  LDD2025  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [5]
 LDCM0116  HHS-0101 DM93 Y207(1.74)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y207(0.85)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y207(0.93)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y207(1.11)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y207(0.37)  LDD0268  [4]
 LDCM0107  IAA HeLa N.A.  LDD0221  [5]
 LDCM0109  NEM HeLa N.A.  LDD0223  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin thioesterase ZRANB1 (ZRANB1) Peptidase C64 family Q9UGI0
SRSF protein kinase 1 (SRPK1) CMGC Ser/Thr protein kinase family Q96SB4
SRSF protein kinase 2 (SRPK2) CMGC Ser/Thr protein kinase family P78362
Dual specificity protein kinase CLK2 (CLK2) CMGC Ser/Thr protein kinase family P49760
Dual specificity protein kinase CLK3 (CLK3) CMGC Ser/Thr protein kinase family P49761
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pinin (PNN) Pinin family Q9H307
Transcription factor
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger and SCAN domain-containing protein 30 (ZSCAN30) Krueppel C2H2-type zinc-finger protein family Q86W11
Zinc finger protein 165 (ZNF165) Krueppel C2H2-type zinc-finger protein family P49910
Zinc finger protein 286A (ZNF286A) Krueppel C2H2-type zinc-finger protein family Q9HBT8
Zinc finger protein 345 (ZNF345) Krueppel C2H2-type zinc-finger protein family Q14585
Zinc finger protein 394 (ZNF394) Krueppel C2H2-type zinc-finger protein family Q53GI3
Zinc finger protein 660 (ZNF660) Krueppel C2H2-type zinc-finger protein family Q6AZW8
Other
Click To Hide/Show 19 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclin-L1 (CCNL1) Cyclin family Q9UK58
Protein FAM81B (FAM81B) FAM81 family Q96LP2
Golgin subfamily A member 6-like protein 9 (GOLGA6L9) GOLGA6 family A6NEM1
Protein HEXIM2 (HEXIM2) HEXIM family Q96MH2
Serine/arginine repetitive matrix protein 4 (SRRM4) NSR100 family A7MD48
Serine/arginine-rich splicing factor 10 (SRSF10) Splicing factor SR family O75494
Serine/arginine-rich splicing factor 3 (SRSF3) Splicing factor SR family P84103
Serine/arginine-rich splicing factor 7 (SRSF7) Splicing factor SR family Q16629
Serine/arginine-rich splicing factor 9 (SRSF9) Splicing factor SR family Q13242
Transformer-2 protein homolog alpha (TRA2A) Splicing factor SR family Q13595
Syntaxin-11 (STX11) Syntaxin family O75558
Elongin-A2 (ELOA2) . Q8IYF1
PRKCA-binding protein (PICK1) . Q9NRD5
Proline-serine-threonine phosphatase-interacting protein 1 (PSTPIP1) . O43586
RNA-binding motif protein, Y chromosome, family 1 member F/J (RBMY1F; RBMY1J) . Q15415
Squamous cell carcinoma antigen recognized by T-cells 3 (SART3) . Q15020
Syntenin-2 (SDCBP2) . Q9H190
Uncharacterized protein NKAPD1 (NKAPD1) . Q6ZUT1
YTH domain-containing protein 1 (YTHDC1) . Q96MU7

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
3 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
7 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
10 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
11 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587