Details of the Target

General Information of Target

Target ID LDTP14045
Target Name Disheveled-associated activator of morphogenesis 1 (DAAM1)
Gene Name DAAM1
Gene ID 23002
Synonyms
KIAA0666; Disheveled-associated activator of morphogenesis 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSRLIVKNLPNGMKEERFRQLFAAFGTLTDCSLKFTKDGKFRKFGFIGFKSEEEAQKAQK
HFNKSFIDTSRITVEFCKSFGDPAKPRAWSKHAQKPSQPKQPPKDSTTPEIKKDEKKKKV
AGQLEKLKEDTEFQEFLSVHQRRAQAATWANDGLDAEPSKGKSKPASDYLNFDSDSGQES
EEEGAGEDLEEEASLEPKAAVQKELSDMDYLKSKMVKAGSSSSSEEEESEDEAVHCDEGS
EAEEEDSSATPVLQERDSKGAGQEQGMPAGKKRPPEARAETEKPANQKEPTTCHTVKLRG
APFNVTEKNVMEFLAPLKPVAIRIVRNAHGNKTGYIFVDFSNEEEVKQALKCNREYMGGR
YIEVFREKNVPTTKGAPKNTTKSWQGRILGENEEEEDLAESGRLFVRNLPYTSTEEDLEK
LFSKYGPLSELHYPIDSLTKKPKGFAFITFMFPEHAVKAYSEVDGQVFQGRMLHVLPSTI
KKEASEDASALGSSSYKKKKEAQDKANSASSHNWNTLFMGPNAVADAIAQKYNATKSQVF
DHETKGSVAVRVALGETQLVQEVRRFLIDNGVSLDSFSQAAAERSKTVILVKNLPAGTLA
AQLQETFGHFGSLGRVLLPEGGITAIVEFLEPLEARKAFRHLAYSKFHHVPLYLEWAPVG
VFSSTAPQKKKLQDTPSEPMEKDPAEPETVPDGETPEDENPTEEGADNSSAKMEEEEEEE
EEEEESLPGCTLFIKNLNFDTTEEKLKEVFSKVGTVKSCSISKKKNKAGVLLSMGFGFVE
YRKPEQAQKALKQLQGHVVDGHKLEVRISERATKPAVTLARKKQVPRKQTTSKILVRNIP
FQAHSREIRELFSTFGELKTVRLPKKMTGTGTHRGFGFVDFLTKQDAKRAFNALCHSTHL
YGRRLVLEWADSEVTLQALRRKTAAHFHEPPKKKRSVVLDEILEQLEGSDSDSEEQTLQL
Target Bioclass
Other
Family
Formin homology family
Subcellular location
Cytoplasm
Function
Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Together with DAAM2, required for myocardial maturation and sarcomere assembly.
Uniprot ID
Q9Y4D1
Ensemble ID
ENST00000360909.8
HGNC ID
HGNC:18142

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P1
 Probe Info  		3.89  LDD0452  [1]
P2
 Probe Info  		2.29  LDD0453  [1]
P3
 Probe Info  		1.68  LDD0454  [1]
P8
 Probe Info  		9.46  LDD0455  [1]
STPyne
 Probe Info  		K138(10.00); K780(1.14)  LDD0277  [2]
BTD
 Probe Info  		C696(0.66)  LDD2122  [3]
DBIA
 Probe Info  		C87(0.87)  LDD1514  [4]
N1
 Probe Info  		E365(0.00); K369(0.00); M363(0.00); T367(0.00)  LDD0245  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [6]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0284  AC24 HEK-293T C87(0.97)  LDD1523  [4]
 LDCM0293  AC32 HEK-293T C87(0.95)  LDD1532  [4]
 LDCM0302  AC40 HEK-293T C87(1.11)  LDD1541  [4]
 LDCM0310  AC48 HEK-293T C87(0.95)  LDD1549  [4]
 LDCM0319  AC56 HEK-293T C87(0.98)  LDD1558  [4]
 LDCM0328  AC64 HEK-293T C87(0.89)  LDD1567  [4]
 LDCM0345  AC8 HEK-293T C87(0.93)  LDD1569  [4]
 LDCM0275  AKOS034007705 HEK-293T C87(0.87)  LDD1514  [4]
 LDCM0369  CL100 HEK-293T C87(0.99)  LDD1573  [4]
 LDCM0373  CL104 HEK-293T C87(1.17)  LDD1577  [4]
 LDCM0377  CL108 HEK-293T C87(0.98)  LDD1581  [4]
 LDCM0382  CL112 HEK-293T C87(0.87)  LDD1586  [4]
 LDCM0386  CL116 HEK-293T C87(1.11)  LDD1590  [4]
 LDCM0390  CL12 HEK-293T C87(1.03)  LDD1594  [4]
 LDCM0391  CL120 HEK-293T C87(1.12)  LDD1595  [4]
 LDCM0395  CL124 HEK-293T C87(1.49)  LDD1599  [4]
 LDCM0399  CL128 HEK-293T C87(0.98)  LDD1603  [4]
 LDCM0403  CL16 HEK-293T C87(1.24)  LDD1607  [4]
 LDCM0412  CL24 HEK-293T C87(1.18)  LDD1616  [4]
 LDCM0416  CL28 HEK-293T C87(1.12)  LDD1620  [4]
 LDCM0425  CL36 HEK-293T C87(1.32)  LDD1629  [4]
 LDCM0429  CL4 HEK-293T C87(1.27)  LDD1633  [4]
 LDCM0430  CL40 HEK-293T C87(1.04)  LDD1634  [4]
 LDCM0438  CL48 HEK-293T C87(1.04)  LDD1642  [4]
 LDCM0443  CL52 HEK-293T C87(1.06)  LDD1646  [4]
 LDCM0452  CL60 HEK-293T C87(1.16)  LDD1655  [4]
 LDCM0456  CL64 HEK-293T C87(1.10)  LDD1659  [4]
 LDCM0465  CL72 HEK-293T C87(1.05)  LDD1668  [4]
 LDCM0469  CL76 HEK-293T C87(1.24)  LDD1672  [4]
 LDCM0478  CL84 HEK-293T C87(1.01)  LDD1681  [4]
 LDCM0482  CL88 HEK-293T C87(0.96)  LDD1685  [4]
 LDCM0491  CL96 HEK-293T C87(0.98)  LDD1694  [4]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C696(0.66)  LDD2122  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transforming protein RhoA (RHOA) Rho family P61586
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Formin-binding protein 1-like (FNBP1L) FNBP1 family Q5T0N5
Disheveled-associated activator of morphogenesis 1 (DAAM1) Formin homology family Q9Y4D1
Protein inturned (INTU) Inturned family Q9ULD6

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853