Details of the Target

General Information of Target

Target ID LDTP04919
Target Name Large ribosomal subunit protein eL15 (RPL15)
Gene Name RPL15
Gene ID 6138
Synonyms
EC45; Large ribosomal subunit protein eL15; 60S ribosomal protein L15
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYV
IYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYW
VGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKF
HHTIGGSRRAAWRRRNTLQLHRYR
Target Type
Literature-reported
Target Bioclass
Other
Family
Eukaryotic ribosomal protein eL15 family
Subcellular location
Cytoplasm
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
TTD ID
T73230
Uniprot ID
P61313
DrugMap ID
TTQRVC9
Ensemble ID
ENST00000307839.10
HGNC ID
HGNC:10306

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCC15 SNV: p.R24S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 31 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		6.38  LDD0402  [1]
P1
 Probe Info  		1.82  LDD0448  [2]
P3
 Probe Info  		2.62  LDD0450  [2]
A-EBA
 Probe Info  		2.76  LDD0215  [3]
C-Sul
 Probe Info  		5.13  LDD0066  [4]
TH211
 Probe Info  		Y81(20.00)  LDD0257  [5]
TH214
 Probe Info  		Y81(5.42)  LDD0258  [5]
TH216
 Probe Info  		Y81(9.09)  LDD0259  [5]
YN-1
 Probe Info  		100.00  LDD0444  [6]
YN-4
 Probe Info  		100.00  LDD0445  [6]
STPyne
 Probe Info  		K153(6.48); K179(8.33); K47(5.26); K56(2.81)  LDD0277  [7]
BTD
 Probe Info  		C110(1.96)  LDD1699  [8]
ONAyne
 Probe Info  		K153(0.00); K83(0.00)  LDD0273  [7]
DBIA
 Probe Info  		C220(1.16)  LDD3330  [9]
ATP probe
 Probe Info  		K83(0.00); K153(0.00); K56(0.00); K93(0.00)  LDD0199  [10]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [11]
IPM
 Probe Info  		N.A.  LDD0005  [12]
OSF
 Probe Info  		N.A.  LDD0029  [13]
SF
 Probe Info  		K83(0.00); Y81(0.00); Y62(0.00)  LDD0028  [13]
TFBX
 Probe Info  		N.A.  LDD0148  [14]
Ox-W18
 Probe Info  		W11(0.00); W120(0.00); W150(0.00); W192(0.00)  LDD2175  [15]
Acrolein
 Probe Info  		H86(0.00); H87(0.00); H182(0.00); H181(0.00)  LDD0217  [16]
Crotonaldehyde
 Probe Info  		H181(0.00); H201(0.00); H182(0.00)  LDD0219  [16]
Methacrolein
 Probe Info  		H181(0.00); H182(0.00)  LDD0218  [16]
AOyne
 Probe Info  		5.40  LDD0443  [17]
MPP-AC
 Probe Info  		N.A.  LDD0428  [18]
NAIA_5
 Probe Info  		C110(0.00); C27(0.00)  LDD2223  [19]
TER-AC
 Probe Info  		N.A.  LDD0426  [18]
TPP-AC
 Probe Info  		N.A.  LDD0427  [18]
HHS-475
 Probe Info  		Y81(1.11)  LDD2238  [20]
HHS-482
 Probe Info  		Y81(1.05)  LDD2239  [20]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-2
 Probe Info  		N.A.  LDD0138  [21]
DA-2
 Probe Info  		N.A.  LDD0072  [22]
STS-1
 Probe Info  		N.A.  LDD0069  [23]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C110(0.66)  LDD2142  [8]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C110(1.11)  LDD2112  [8]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C110(0.82)  LDD2095  [8]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C110(0.63)  LDD2130  [8]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C110(0.84)  LDD2117  [8]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C110(1.33)  LDD2152  [8]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C110(0.48)  LDD2131  [8]
 LDCM0545  Acetamide MDA-MB-231 C110(0.73)  LDD2138  [8]
 LDCM0520  AKOS000195272 MDA-MB-231 C110(0.91)  LDD2113  [8]
 LDCM0498  BS-3668 MDA-MB-231 C110(0.72)  LDD2091  [8]
 LDCM0108  Chloroacetamide HeLa H87(0.00); H182(0.00); H86(0.00); H181(0.00)  LDD0222  [16]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C110(0.90)  LDD1702  [8]
 LDCM0625  F8 Ramos C27(0.84)  LDD2187  [24]
 LDCM0572  Fragment10 Ramos C27(0.56)  LDD2189  [24]
 LDCM0573  Fragment11 Ramos C27(0.02)  LDD2190  [24]
 LDCM0574  Fragment12 Ramos C27(0.34)  LDD2191  [24]
 LDCM0575  Fragment13 Ramos C27(0.63)  LDD2192  [24]
 LDCM0576  Fragment14 Ramos C27(0.44)  LDD2193  [24]
 LDCM0579  Fragment20 Ramos C27(0.28)  LDD2194  [24]
 LDCM0580  Fragment21 Ramos C27(0.60)  LDD2195  [24]
 LDCM0582  Fragment23 Ramos C27(1.19)  LDD2196  [24]
 LDCM0578  Fragment27 Ramos C27(0.72)  LDD2197  [24]
 LDCM0586  Fragment28 Ramos C27(1.06)  LDD2198  [24]
 LDCM0588  Fragment30 Ramos C27(1.08)  LDD2199  [24]
 LDCM0589  Fragment31 Ramos C27(0.83)  LDD2200  [24]
 LDCM0590  Fragment32 Ramos C27(0.40)  LDD2201  [24]
 LDCM0468  Fragment33 Ramos C27(0.95)  LDD2202  [24]
 LDCM0596  Fragment38 Ramos C27(1.35)  LDD2203  [24]
 LDCM0566  Fragment4 Ramos C27(0.89)  LDD2184  [24]
 LDCM0610  Fragment52 Ramos C27(0.57)  LDD2204  [24]
 LDCM0614  Fragment56 Ramos C27(0.83)  LDD2205  [24]
 LDCM0569  Fragment7 Ramos C27(0.21)  LDD2186  [24]
 LDCM0571  Fragment9 Ramos C27(0.44)  LDD2188  [24]
 LDCM0107  IAA HeLa H87(0.00); H182(0.00); H86(0.00); H201(0.00)  LDD0221  [16]
 LDCM0022  KB02 Ramos C27(0.21)  LDD2182  [24]
 LDCM0023  KB03 MDA-MB-231 C110(1.17)  LDD1701  [8]
 LDCM0024  KB05 MIA PaCa-2 C220(1.16)  LDD3330  [9]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C110(0.96)  LDD2121  [8]
 LDCM0109  NEM HeLa H87(0.00); H86(0.00); H156(0.00); H182(0.00)  LDD0223  [16]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C110(0.84)  LDD2089  [8]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C110(0.95)  LDD2092  [8]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C110(1.07); C27(0.88)  LDD2093  [8]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C110(1.25)  LDD2094  [8]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C110(0.50)  LDD2096  [8]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C110(1.13)  LDD2097  [8]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C110(0.93)  LDD2098  [8]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C110(1.14)  LDD2099  [8]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C110(0.58)  LDD2100  [8]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C110(0.92)  LDD2101  [8]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C110(0.69)  LDD2104  [8]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C110(0.97); C27(1.46)  LDD2107  [8]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C110(0.82)  LDD2108  [8]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C110(0.54); C27(0.46)  LDD2109  [8]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C110(0.94)  LDD2111  [8]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C110(0.81)  LDD2114  [8]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C110(0.52)  LDD2115  [8]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C110(0.96)  LDD2116  [8]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C110(0.82)  LDD2118  [8]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C110(1.91); C27(1.64)  LDD2119  [8]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C110(0.70)  LDD2120  [8]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C110(0.64); C27(0.30)  LDD2122  [8]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C110(0.91); C27(0.75)  LDD2123  [8]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C110(0.57); C27(0.33)  LDD2124  [8]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C110(0.81); C27(0.73)  LDD2125  [8]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C110(0.81)  LDD2126  [8]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C110(1.06); C27(1.34)  LDD2127  [8]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C110(0.74)  LDD2128  [8]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C110(1.31); C27(1.59)  LDD2129  [8]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C110(0.58)  LDD2133  [8]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C110(0.55)  LDD2134  [8]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C110(1.65); C27(2.06)  LDD2135  [8]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C110(1.16); C27(0.99)  LDD2136  [8]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C110(0.96); C27(0.90)  LDD2137  [8]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C110(1.90)  LDD1700  [8]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C27(0.80)  LDD2140  [8]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C110(0.71)  LDD2141  [8]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C110(0.73)  LDD2143  [8]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C110(2.15); C27(2.31)  LDD2144  [8]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C27(0.28)  LDD2145  [8]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C110(0.83)  LDD2146  [8]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C110(1.84)  LDD2147  [8]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C110(0.61)  LDD2148  [8]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C110(0.88)  LDD2149  [8]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C110(0.54)  LDD2150  [8]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C110(0.66)  LDD2151  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein MOX-2 (MEOX2) . P50222
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 3 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
(S)-3-phenyllactic Acid . DB02494
Anisomycin . DB07374
Puromycin . DB08437

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
3 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
4 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
5 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
6 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
7 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
8 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
9 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
12 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
13 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
14 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
15 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
16 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
17 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
18 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.
19 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
20 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
21 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
22 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
23 Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe. Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.
24 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578