Details of the Target

General Information of Target

Target ID LDTP04974
Target Name Small ribosomal subunit protein uS15 (RPS13)
Gene Name RPS13
Gene ID 6207
Synonyms
Small ribosomal subunit protein uS15; 40S ribosomal protein S13
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGV
AQVRFVTGNKILRILKSKGLAPDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIES
RIHRLARYYKTKRVLPPNWKYESSTASALVA
Target Bioclass
Other
Family
Universal ribosomal protein uS15 family
Subcellular location
Cytoplasm
Function
Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Uniprot ID
P62277
Ensemble ID
ENST00000525634.6
HGNC ID
HGNC:10386

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 28 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
P2
 Probe Info  		1.54  LDD0449  [1]
P3
 Probe Info  		1.51  LDD0454  [1]
C-Sul
 Probe Info  		5.64  LDD0066  [2]
TH211
 Probe Info  		Y18(12.51); Y89(12.40); Y38(11.48)  LDD0257  [3]
TH214
 Probe Info  		Y89(6.30)  LDD0258  [3]
TH216
 Probe Info  		Y89(20.00); Y18(13.91)  LDD0259  [3]
YN-4
 Probe Info  		100.00  LDD0445  [4]
ONAyne
 Probe Info  		N.A.  LDD0273  [5]
OPA-S-S-alkyne
 Probe Info  		K9(2.45); K70(6.69); K43(7.76)  LDD3494  [6]
Probe 1
 Probe Info  		Y18(22.19)  LDD3495  [7]
HHS-482
 Probe Info  		Y18(0.93); Y38(0.98); Y89(0.88)  LDD0285  [8]
HHS-475
 Probe Info  		Y89(0.57); Y38(7.41)  LDD0264  [9]
HHS-465
 Probe Info  		Y18(5.34); Y38(6.70); Y89(3.61)  LDD2237  [10]
5E-2FA
 Probe Info  		N.A.  LDD2235  [11]
AMP probe
 Probe Info  		K78(0.00); K43(0.00); K39(0.00); K34(0.00)  LDD0200  [12]
ATP probe
 Probe Info  		K78(0.00); K43(0.00); K39(0.00); K34(0.00)  LDD0199  [12]
m-APA
 Probe Info  		N.A.  LDD2232  [11]
1d-yne
 Probe Info  		N.A.  LDD0356  [13]
NHS
 Probe Info  		K27(0.00); K78(0.00); K34(0.00); K43(0.00)  LDD0010  [14]
OSF
 Probe Info  		Y38(0.00); H58(0.00); Y18(0.00)  LDD0029  [15]
SF
 Probe Info  		Y18(0.00); K34(0.00); K93(0.00); Y38(0.00)  LDD0028  [15]
STPyne
 Probe Info  		K34(0.00); K43(0.00); K27(0.00)  LDD0009  [14]
Ox-W18
 Probe Info  		N.A.  LDD2175  [16]
1c-yne
 Probe Info  		K9(0.00); K27(0.00); K76(0.00); K78(0.00)  LDD0228  [13]
Acrolein
 Probe Info  		H90(0.00); H58(0.00)  LDD0217  [17]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [17]
Methacrolein
 Probe Info  		N.A.  LDD0218  [17]
W1
 Probe Info  		K78(0.00); D56(0.00); S77(0.00)  LDD0236  [18]
PAL-AfBPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C003
 Probe Info  		12.38  LDD1713  [19]
C228
 Probe Info  		36.50  LDD1901  [19]
FFF probe13
 Probe Info  		5.04  LDD0475  [20]
STS-2
 Probe Info  		N.A.  LDD0138  [21]
Photocelecoxib
 Probe Info  		N.A.  LDD0153  [22]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H90(0.00); H58(0.00)  LDD0222  [17]
 LDCM0116  HHS-0101 DM93 Y89(0.57); Y38(7.41)  LDD0264  [9]
 LDCM0117  HHS-0201 DM93 Y89(1.26)  LDD0265  [9]
 LDCM0118  HHS-0301 DM93 Y38(0.29); Y89(0.44)  LDD0266  [9]
 LDCM0119  HHS-0401 DM93 Y38(0.27); Y89(0.96)  LDD0267  [9]
 LDCM0120  HHS-0701 DM93 Y38(0.23); Y89(0.57)  LDD0268  [9]
 LDCM0107  IAA HeLa H90(0.00); H58(0.00)  LDD0221  [17]
 LDCM0123  JWB131 DM93 Y18(0.93); Y38(0.98); Y89(0.88)  LDD0285  [8]
 LDCM0124  JWB142 DM93 Y18(0.69); Y38(0.92); Y89(0.77)  LDD0286  [8]
 LDCM0125  JWB146 DM93 Y18(1.06); Y38(1.39); Y89(0.97)  LDD0287  [8]
 LDCM0126  JWB150 DM93 Y18(2.37); Y38(3.35); Y89(3.30)  LDD0288  [8]
 LDCM0127  JWB152 DM93 Y18(1.76); Y38(2.25); Y89(2.15)  LDD0289  [8]
 LDCM0128  JWB198 DM93 Y18(1.06); Y38(1.31); Y89(1.36)  LDD0290  [8]
 LDCM0129  JWB202 DM93 Y18(0.44); Y38(0.59); Y89(0.58)  LDD0291  [8]
 LDCM0130  JWB211 DM93 Y18(0.90); Y38(1.23); Y89(1.08)  LDD0292  [8]
 LDCM0109  NEM HeLa H90(0.00); H58(0.00)  LDD0223  [17]
 LDCM0112  W16 Hep-G2 N69(0.96)  LDD0239  [18]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitogen-activated protein kinase kinase kinase 14 (MAP3K14) STE Ser/Thr protein kinase family Q99558
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) TKL Ser/Thr protein kinase family Q5S007
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Programmed cell death protein 4 (PDCD4) PDCD4 family Q53EL6

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Artenimol . DB11638

References

1 Comparison of Different Competitive Proteome Profiling Approaches in Target Identification of Covalent Inhibitors. Chembiochem. 2022 Dec 16;23(24):e202200389. doi: 10.1002/cbic.202200389. Epub 2022 Nov 22.
2 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
5 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
6 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
7 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
8 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
9 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
10 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
11 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
12 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
13 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
14 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
15 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
16 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
17 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
18 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
19 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
20 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
21 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
22 Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J Am Chem Soc. 2018 Mar 28;140(12):4259-4268. doi: 10.1021/jacs.7b11639. Epub 2018 Mar 15.
Mass spectrometry data entry: PXD007094