Details of the Probe

General Information of Probe

Probe ID
LDPC0273
Probe Name
C003
Synonyms
Z4286591582
Probe Type
PAL-AfBPP Probe
Structure
3D MOL 2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 381.4
Lipid-water partition coefficient (xlogp) 3.1
Rotatable Bond Count (rotbonds) 8
Hydrogen Bond Donor Count (hbonddonor) 1
Hydrogen Bond Acceptor Count (hbondacc) 6
Chemical Identifiers
Formula
C21H23N3O4
IUPAC Name
N-[2-(3-but-3-ynyldiazirin-3-yl)ethyl]-2-(3,4,8-trimethyl-2-oxochromen-7-yl)oxyacetamide
Canonical SMILES
CC1=C(C(=O)OC2=C1C=CC(=C2C)OCC(=O)NCCC3(N=N3)CCC#C)C
InChI
InChI=1S/C21H23N3O4/c1-5-6-9-21(23-24-21)10-11-22-18(25)12-27-17-8-7-16-13(2)14(3)20(26)28-19(16)15(17)4/h1,7-8H,6,9-12H2,2-4H3,(H,22,25)
InChIKey
CYZLXMLODRBOBY-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

28 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Adenosine kinase (ADK) LDTP04689 20.39  LDD1713 
ATP synthase subunit beta, mitochondrial (ATP5F1B) LDTP02150 14.42  LDD1713 
Biliverdin reductase A (BLVRA) LDTP04552 17.51  LDD1713 
Ceramide synthase 2 (CERS2) LDTP10329 14.93  LDD1713 
Creatine kinase U-type, mitochondrial (CKMT1A; CKMT1B) LDTP02622 12.13  LDD1713 
Cullin-5 (CUL5) LDTP09965 13.36  LDD1713 
Cytochrome b-c1 complex subunit 7 (UQCRB) LDTP02757 17.88  LDD1713 
DCN1-like protein 4 (DCUN1D4) LDTP09799 22.47  LDD1713 
DCN1-like protein 5 (DCUN1D5) LDTP11199 13.00  LDD1713 
dCTP pyrophosphatase 1 (DCTPP1) LDTP12013 12.82  LDD1713 
E3 SUMO-protein ligase NSE2 (NSMCE2) LDTP10583 15.24  LDD1713 
EH domain-containing protein 4 (EHD4) LDTP11788 12.82  LDD1713 
Haloacid dehalogenase-like hydrolase domain-containing protein 3 (HDHD3) LDTP15879 14.52  LDD1713 
Heme oxygenase 1 (HMOX1) LDTP02359 12.82  LDD1713 
Heme oxygenase 2 (HMOX2) LDTP03540 14.03  LDD1713 
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (MAN1A2) LDTP00975 13.64  LDD1713 
N-alpha-acetyltransferase 40 (NAA40) LDTP08265 15.89  LDD1713 
NADH dehydrogenase 1 alpha subcomplex subunit 13 (NDUFA13) LDTP12959 14.03  LDD1713 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 23.92  LDD1713 
Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) LDTP12628 16.11  LDD1713 
Prostaglandin reductase 3 (PTGR3) LDTP15422 21.26  LDD1713 
Proteasome subunit beta type-4 (PSMB4) LDTP03417 14.42  LDD1713 
Protein-S-isoprenylcysteine O-methyltransferase (ICMT) LDTP01039 11.96  LDD1713 
Pyridoxine-5'-phosphate oxidase (PNPO) LDTP12691 12.91  LDD1713 
Squalene synthase (FDFT1) LDTP03842 14.93  LDD1713 
Thymidylate synthase (TYMS) LDTP02055 13.18  LDD1713 
Translin (TSN) LDTP06427 12.55  LDD1713 
UDP-glucose 4-epimerase (GALE) LDTP06111 12.82  LDD1713 
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⏷ Show the Full List of 28 Enzyme
12 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
14-3-3 protein epsilon (YWHAE) LDTP04969 12.91  LDD1713 
45 kDa calcium-binding protein (SDF4) LDTP11116 15.78  LDD1713 
ATP synthase subunit g, mitochondrial (ATP5MG) LDTP14511 28.44  LDD1713 
Cytoskeleton-associated protein 4 (CKAP4) LDTP05511 12.47  LDD1713 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 18.13  LDD1713 
Golgin subfamily A member 5 (GOLGA5) LDTP09333 13.00  LDD1713 
Melanoma inhibitory activity protein 2 (MIA2) LDTP10669 15.24  LDD1713 
Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) LDTP13983 15.35  LDD1713 
Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) LDTP01059 13.36  LDD1713 
StAR-related lipid transfer protein 3 (STARD3) LDTP06221 29.24  LDD1713 
Transmembrane protein 126A (TMEM126A) LDTP15948 14.52  LDD1713 
Transmembrane protein 199 (TMEM199) LDTP08881 13.36  LDD1713 
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⏷ Show the Full List of 12 Transporter and channel
4 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
High mobility group protein 20A (HMG20A) LDTP12309 12.13  LDD1713 
POU domain, class 2, transcription factor 1 (POU2F1) LDTP02749 13.09  LDD1713 
Splicing factor, proline- and glutamine-rich (SFPQ) LDTP03212 15.24  LDD1713 
Transcription factor A, mitochondrial (TFAM) LDTP05235 13.74  LDD1713 
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1 Immunoglobulin Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Basigin (BSG) LDTP03772 15.35  LDD1713 
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48 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Alpha-internexin (INA) LDTP06527 12.13  LDD1713 
Apolipoprotein L2 (APOL2) LDTP11076 22.16  LDD1713 
c-Myc-binding protein (MYCBP) LDTP10857 16.91  LDD1713 
Calumenin (CALU) LDTP00887 13.83  LDD1713 
Centrin-2 (CETN2) LDTP03926 16.22  LDD1713 
Clustered mitochondria protein homolog (CLUH) LDTP14486 12.91  LDD1713 
Coiled-coil domain-containing protein 124 (CCDC124) LDTP10187 13.27  LDD1713 
Craniofacial development protein 1 (CFDP1) LDTP16193 14.62  LDD1713 
Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) LDTP13902 13.83  LDD1713 
DNA replication complex GINS protein SLD5 (GINS4) LDTP11134 15.78  LDD1713 
DnaJ homolog subfamily C member 8 (DNAJC8) LDTP01361 14.52  LDD1713 
Elongation factor 1-delta (EEF1D) LDTP03499 12.64  LDD1713 
Endothelial differentiation-related factor 1 (EDF1) LDTP01067 33.36  LDD1713 
Ezrin (EZR) LDTP02779 11.96  LDD1713 
Keratin, type I cytoskeletal 18 (KRT18) LDTP02124 22.78  LDD1713 
Lamin-B1 (LMNB1) LDTP03065 12.47  LDD1713 
Lamin-B2 (LMNB2) LDTP05400 14.83  LDD1713 
Liprin-alpha-1 (PPFIA1) LDTP05763 13.64  LDD1713 
Lupus La protein (SSB) LDTP02116 12.30  LDD1713 
Mammalian ependymin-related protein 1 (EPDR1) LDTP13606 15.24  LDD1713 
Microfibrillar-associated protein 1 (MFAP1) LDTP04668 11.96  LDD1713 
Mitochondrial import inner membrane translocase subunit Tim8 B (TIMM8B) LDTP16322 12.13  LDD1713 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 22.16  LDD1713 
NADH dehydrogenase 1 alpha subcomplex assembly factor 2 (NDUFAF2) LDTP08761 12.91  LDD1713 
Neurofilament medium polypeptide (NEFM) LDTP02181 13.45  LDD1713 
Nucleobindin-2 (NUCB2) LDTP05160 13.00  LDD1713 
Nucleolar protein 16 (NOP16) LDTP16278 13.00  LDD1713 
Pre-mRNA-splicing factor SLU7 (SLU7) LDTP01582 12.82  LDD1713 
Prefoldin subunit 6 (PFDN6) LDTP00573 18.25  LDD1713 
Prelamin-A/C (LMNA) LDTP01932 13.93  LDD1713 
Prohibitin-2 (PHB2) LDTP10924 11.96  LDD1713 
Protein bicaudal D homolog 2 (BICD2) LDTP09410 13.93  LDD1713 
Protein S100-A13 (S100A13) LDTP10908 14.42  LDD1713 
Putative RNA-binding protein Luc7-like 1 (LUC7L) LDTP12388 13.64  LDD1713 
Radixin (RDX) LDTP03721 15.67  LDD1713 
Regulator of G-protein signaling 10 (RGS10) LDTP00829 13.45  LDD1713 
Required for meiotic nuclear division protein 1 homolog (RMND1) LDTP12729 16.45  LDD1713 
Sarcolemmal membrane-associated protein (SLMAP) LDTP06254 12.04  LDD1713 
Small ribosomal subunit protein uS15 (RPS13) LDTP04974 12.38  LDD1713 
Splicing factor 3B subunit 4 (SF3B4) LDTP06386 14.03  LDD1713 
Stathmin (STMN1) LDTP02869 21.56  LDD1713 
Torsin-1A-interacting protein 2 (TOR1AIP2) LDTP09215 16.11  LDD1713 
Tropomyosin alpha-1 chain (TPM1) LDTP02351 24.59  LDD1713 
Tropomyosin alpha-4 chain (TPM4) LDTP05068 17.39  LDD1713 
Tumor protein D52 (TPD52) LDTP04703 15.14  LDD1713 
Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) LDTP15067 14.62  LDD1713 
Ubiquitin-like protein 4A (UBL4A) LDTP02570 13.45  LDD1713 
Vimentin (VIM) LDTP02297 19.43  LDD1713 
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⏷ Show the Full List of 48 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD1713 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587