Details of the Target

General Information of Target

Target ID LDTP03721
Target Name Radixin (RDX)
Gene Name RDX
Gene ID 5962
Synonyms
Radixin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTVGLREVWFFGLQYVDSKGYSTWLK
LNKKVTQQDVKKENPLQFKFRAKFFPEDVSEELIQEITQRLFFLQVKEAILNDEIYCPPE
TAVLLASYAVQAKYGDYNKEIHKPGYLANDRLLPQRVLEQHKLTKEQWEERIQNWHEEHR
GMLREDSMMEYLKIAQDLEMYGVNYFEIKNKKGTELWLGVDALGLNIYEHDDKLTPKIGF
PWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTI
EVQQMKAQAREEKHQKQLERAQLENEKKKREIAEKEKERIEREKEELMERLKQIEEQTIK
AQKELEEQTRKALELDQERKRAKEEAERLEKERRAAEEAKSAIAKQAADQMKNQEQLAAE
LAEFTAKIALLEEAKKKKEEEATEWQHKAFAAQEDLEKTKEELKTVMSAPPPPPPPPVIP
PTENEHDEHDENNAEASAELSNEGVMNHRSEEERVTETQKNERVKKQLQALSSELAQARD
ETKKTQNDVLHAENVKAGRDKYKTLRQIRQGNTKQRIDEFEAM
Target Bioclass
Other
Subcellular location
Cell membrane
Function Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Uniprot ID
P35241
Ensemble ID
ENST00000528498.5
HGNC ID
HGNC:9944

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.Y116H; p.V478A .
MCC13 SNV: p.R370Ter .
MEWO SNV: p.P86S DBIA    Probe Info 
NUGC3 Deletion: p.P471LfsTer35 DBIA    Probe Info 
OVCAR8 SNV: p.Q529Ter DBIA    Probe Info 
SW948 SNV: p.K563N DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 20 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
ONAyne
 Probe Info  		K400(6.52); K520(5.26)  LDD0274  [2]
STPyne
 Probe Info  		K165(0.76); K400(2.29); K405(10.00); K435(7.73)  LDD0277  [2]
AZ-9
 Probe Info  		E415(1.37); E178(0.82); D455(10.00)  LDD2208  [3]
OPA-S-S-alkyne
 Probe Info  		K371(1.33); K363(1.44); K556(1.52); K520(1.76)  LDD3494  [4]
Probe 1
 Probe Info  		Y55(37.36)  LDD3495  [5]
DBIA
 Probe Info  		C284(2.15)  LDD3310  [6]
HHS-475
 Probe Info  		Y55(0.58); Y270(1.13); Y291(4.80)  LDD0264  [7]
HHS-465
 Probe Info  		Y270(10.00); Y55(3.05)  LDD2237  [8]
Acrolein
 Probe Info  		N.A.  LDD0221  [9]
5E-2FA
 Probe Info  		H142(0.00); H447(0.00); H551(0.00)  LDD2235  [10]
ATP probe
 Probe Info  		N.A.  LDD0199  [11]
m-APA
 Probe Info  		H230(0.00); H551(0.00); H447(0.00); H161(0.00)  LDD2231  [10]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [12]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [13]
NHS
 Probe Info  		K263(0.00); K79(0.00)  LDD0010  [14]
SF
 Probe Info  		K3(0.00); Y270(0.00); K263(0.00); Y55(0.00)  LDD0028  [15]
Ox-W18
 Probe Info  		N.A.  LDD2175  [16]
1c-yne
 Probe Info  		N.A.  LDD0228  [17]
1d-yne
 Probe Info  		N.A.  LDD0357  [17]
PAL-AfBPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C003
 Probe Info  		15.67  LDD1713  [18]
FFF probe13
 Probe Info  		7.70  LDD0475  [19]
FFF probe14
 Probe Info  		7.96  LDD0477  [19]
STS-2
 Probe Info  		N.A.  LDD0138  [20]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [9]
 LDCM0116  HHS-0101 DM93 Y55(0.58); Y270(1.13); Y291(4.80)  LDD0264  [7]
 LDCM0117  HHS-0201 DM93 Y270(0.91); Y55(1.00); Y291(1.63)  LDD0265  [7]
 LDCM0118  HHS-0301 DM93 Y270(0.83); Y55(0.87); Y291(2.50)  LDD0266  [7]
 LDCM0119  HHS-0401 DM93 Y55(1.41); Y270(2.30); Y291(2.38)  LDD0267  [7]
 LDCM0120  HHS-0701 DM93 Y55(0.81); Y270(0.92); Y291(2.97)  LDD0268  [7]
 LDCM0107  IAA HeLa N.A.  LDD0221  [9]
 LDCM0022  KB02 22RV1 C284(1.67)  LDD2243  [6]
 LDCM0023  KB03 22RV1 C284(2.41)  LDD2660  [6]
 LDCM0024  KB05 COLO792 C284(2.15)  LDD3310  [6]
 LDCM0109  NEM HeLa N.A.  LDD0223  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Kin of IRRE-like protein 3 (KIRREL3) Immunoglobulin superfamily Q8IZU9
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Integrin beta-2 (ITGB2) Integrin beta chain family P05107
Ezrin (EZR) . P15311

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
1d-myo-inositol 145-trisphosphate Small molecular drug DB03401

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
4 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
5 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
7 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
8 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
9 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
10 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
11 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
12 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
13 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
14 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
15 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
16 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
17 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
18 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
19 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
20 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.