Details of the Target

General Information of Target

Target ID LDTP03417
Target Name Proteasome subunit beta type-4 (PSMB4)
Gene Name PSMB4
Gene ID 5692
Synonyms
PROS26; Proteasome subunit beta type-4; 26 kDa prosomal protein; HsBPROS26; PROS-26; Macropain beta chain; Multicatalytic endopeptidase complex beta chain; Proteasome beta chain; Proteasome chain 3; HsN3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPITRTQNPMVTGTSVLGVK
FEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEE
LLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAP
SLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQIATVTEK
GVEIEGPLSTETNWDIAHMISGFE
Target Bioclass
Enzyme
Family
Peptidase T1B family
Subcellular location
Cytoplasm
Function
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Uniprot ID
P28070
Ensemble ID
ENST00000290541.7
HGNC ID
HGNC:9541
ChEMBL ID
CHEMBL4879435

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
STPyne
 Probe Info  		K109(8.90)  LDD2217  [2]
AZ-9
 Probe Info  		E239(1.42)  LDD2208  [3]
ONAyne
 Probe Info  		N.A.  LDD0273  [4]
OPA-S-S-alkyne
 Probe Info  		K201(3.87)  LDD3494  [5]
m-APA
 Probe Info  		11.34  LDD0403  [6]
HHS-482
 Probe Info  		Y107(0.17)  LDD0286  [7]
HHS-475
 Probe Info  		Y107(0.74); Y75(0.83)  LDD0264  [8]
HHS-465
 Probe Info  		Y75(3.83)  LDD2237  [9]
1d-yne
 Probe Info  		N.A.  LDD0358  [10]
1c-yne
 Probe Info  		N.A.  LDD0228  [10]
PAL-AfBPP Probe
Click To Hide/Show 61 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C001
 Probe Info  		5.13  LDD1711  [11]
C003
 Probe Info  		14.42  LDD1713  [11]
C008
 Probe Info  		5.21  LDD1717  [11]
C017
 Probe Info  		6.19  LDD1725  [11]
C041
 Probe Info  		9.38  LDD1741  [11]
C059
 Probe Info  		9.58  LDD1756  [11]
C063
 Probe Info  		14.42  LDD1760  [11]
C064
 Probe Info  		6.41  LDD1761  [11]
C070
 Probe Info  		9.99  LDD1766  [11]
C085
 Probe Info  		9.71  LDD1777  [11]
C087
 Probe Info  		32.45  LDD1779  [11]
C089
 Probe Info  		7.46  LDD1781  [11]
C108
 Probe Info  		9.51  LDD1795  [11]
C111
 Probe Info  		6.23  LDD1798  [11]
C147
 Probe Info  		7.36  LDD1829  [11]
C158
 Probe Info  		9.65  LDD1838  [11]
C161
 Probe Info  		17.15  LDD1841  [11]
C166
 Probe Info  		9.13  LDD1846  [11]
C179
 Probe Info  		7.36  LDD1858  [11]
C182
 Probe Info  		6.23  LDD1860  [11]
C187
 Probe Info  		22.94  LDD1865  [11]
C195
 Probe Info  		5.54  LDD1871  [11]
C197
 Probe Info  		7.73  LDD1873  [11]
C220
 Probe Info  		13.83  LDD1894  [11]
C244
 Probe Info  		26.54  LDD1917  [11]
C249
 Probe Info  		13.00  LDD1922  [11]
C256
 Probe Info  		5.13  LDD1929  [11]
C284
 Probe Info  		22.47  LDD1954  [11]
C288
 Probe Info  		7.94  LDD1958  [11]
C293
 Probe Info  		22.47  LDD1963  [11]
C303
 Probe Info  		11.71  LDD1972  [11]
C317
 Probe Info  		10.20  LDD1983  [11]
C322
 Probe Info  		6.36  LDD1988  [11]
C328
 Probe Info  		7.52  LDD1992  [11]
C333
 Probe Info  		7.21  LDD1996  [11]
C338
 Probe Info  		17.27  LDD2001  [11]
C344
 Probe Info  		16.22  LDD2006  [11]
C346
 Probe Info  		44.02  LDD2007  [11]
C351
 Probe Info  		5.62  LDD2012  [11]
C353
 Probe Info  		9.58  LDD2014  [11]
C354
 Probe Info  		15.35  LDD2015  [11]
C364
 Probe Info  		23.75  LDD2025  [11]
C379
 Probe Info  		5.54  LDD2038  [11]
C383
 Probe Info  		15.67  LDD2042  [11]
C387
 Probe Info  		6.23  LDD2046  [11]
C391
 Probe Info  		25.46  LDD2050  [11]
C393
 Probe Info  		4.96  LDD2052  [11]
C399
 Probe Info  		8.94  LDD2058  [11]
C403
 Probe Info  		14.12  LDD2061  [11]
C407
 Probe Info  		11.16  LDD2064  [11]
C429
 Probe Info  		13.93  LDD2084  [11]
C430
 Probe Info  		8.46  LDD2085  [11]
FFF probe12
 Probe Info  		7.73  LDD0473  [12]
FFF probe13
 Probe Info  		20.00  LDD0475  [12]
FFF probe14
 Probe Info  		20.00  LDD0477  [12]
FFF probe2
 Probe Info  		5.92  LDD0463  [12]
FFF probe3
 Probe Info  		17.32  LDD0465  [12]
FFF probe6
 Probe Info  		6.04  LDD0468  [12]
JN0003
 Probe Info  		20.00  LDD0469  [12]
DA-2
 Probe Info  		N.A.  LDD0070  [13]
OEA-DA
 Probe Info  		13.61  LDD0046  [14]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0156  Aniline NCI-H1299 11.34  LDD0403  [6]
 LDCM0116  HHS-0101 DM93 Y107(0.74); Y75(0.83)  LDD0264  [8]
 LDCM0117  HHS-0201 DM93 Y75(0.87); Y107(1.03)  LDD0265  [8]
 LDCM0118  HHS-0301 DM93 Y75(0.91); Y107(1.21)  LDD0266  [8]
 LDCM0119  HHS-0401 DM93 Y75(0.90); Y107(1.11)  LDD0267  [8]
 LDCM0120  HHS-0701 DM93 Y75(1.03); Y107(1.19)  LDD0268  [8]
 LDCM0124  JWB142 DM93 Y107(0.17)  LDD0286  [7]
 LDCM0126  JWB150 DM93 Y107(2.34)  LDD0288  [7]
 LDCM0127  JWB152 DM93 Y107(1.58)  LDD0289  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Maspardin (SPG21) AB hydrolase superfamily Q9NZD8
Probable E3 ubiquitin-protein ligase DTX2 (DTX2) Deltex family Q86UW9
Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) P4HA family Q7Z4N8
Proteasome subunit alpha type-3 (PSMA3) Peptidase T1A family P25788
Proteasome subunit beta type-1 (PSMB1) Peptidase T1B family P20618
Serine/threonine-protein kinase N1 (PKN1) AGC Ser/Thr protein kinase family Q16512
MTRF1L release factor glutamine methyltransferase (HEMK1) Protein N5-glutamine methyltransferase family Q9Y5R4
Fibronectin type III and SPRY domain-containing protein 2 (FSD2) . A1L4K1
Transcription factor
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transcription factor AP-2-delta (TFAP2D) AP-2 family Q7Z6R9
Mothers against decapentaplegic homolog 1 (SMAD1) Dwarfin/SMAD family Q15797
Homeobox protein prophet of Pit-1 (PROP1) Paired homeobox family O75360
B-cell lymphoma 6 protein (BCL6) . P41182
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 (SOHLH1) . Q5JUK2
Other
Click To Hide/Show 13 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) ATG8 family Q9H0R8
Ataxin-1 (ATXN1) ATXN1 family P54253
CCR4-NOT transcription complex subunit 2 (CNOT2) CNOT2/3/5 family Q9NZN8
Keratin-associated protein 19-5 (KRTAP19-5) KRTAP type 19 family Q3LI72
Myozenin-3 (MYOZ3) Myozenin family Q8TDC0
Prefoldin subunit 5 (PFDN5) Prefoldin subunit alpha family Q99471
Tektin-5 (TEKT5) Tektin family Q96M29
AFG2-interacting ribosome maturation factor (AIRIM) . Q9NX04
Grancalcin (GCA) . P28676
Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) . O14964
KN motif and ankyrin repeat domain-containing protein 2 (KANK2) . Q63ZY3
RING finger protein 11 (RNF11) . Q9Y3C5
TAR DNA-binding protein 43 (TARDBP) . Q13148

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
(3ar6r6as)-6-((S)-((S)-cyclohex-2-enyl)(Hydroxy)Methyl)-6a-methyl-4-oxo-hexahydro-2h-furo[32-c]Pyrrole-6-carbaldehyde . DB08515

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 Global profiling of lysine reactivity and ligandability in the human proteome. Nat Chem. 2017 Dec;9(12):1181-1190. doi: 10.1038/nchem.2826. Epub 2017 Jul 31.
3 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
4 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
5 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
6 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
7 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
8 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
9 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
10 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
11 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
12 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
13 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
14 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570