Probe Name	C001					Probe Info
Probe concentration	Probe:50uM; negative probe:50uM
Criteria	Quantification: AfBPP Probe vs Negative Probe
Quantitative Method	TMT
In Vivo Experiment Model	Model Type			Living cell
	Derived Tissue			Peripheral blood
	Disease Model		Normal
	Model Name		Human normal cells (HEK-293T)
Note	Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Target ID	Target Name					Binding Ratio
LDTP06333	Serum paraoxonase/arylesterase 2 (PON2)					27.3
LDTP06618	ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP)					20.7
LDTP04285	Proteasome subunit beta type-2 (PSMB2)					16.2
LDTP00575	Glutathione S-transferase A4 (GSTA4)					16.1
LDTP04388	Palmitoyl-protein thioesterase 1 (PPT1)					14.3
LDTP05493	Sulfotransferase 2A1 (SULT2A1)					14.2
LDTP09799	DCN1-like protein 4 (DCUN1D4)					13.5
LDTP04051	Nicotinamide phosphoribosyltransferase (NAMPT)					12.8
LDTP13358	Leucyl-cystinyl aminopeptidase (LNPEP)					12.3
LDTP03540	Heme oxygenase 2 (HMOX2)					12.0
LDTP01982	NADH-ubiquinone oxidoreductase chain 1 (MT-ND1)					11.7
LDTP16032	Retinoid-inducible serine carboxypeptidase (SCPEP1)					10.8
LDTP02359	Heme oxygenase 1 (HMOX1)					10.3
LDTP12020	Histone-lysine N-methyltransferase SMYD3 (SMYD3)					9.8
LDTP14030	V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2)					9.6
LDTP02505	Lysosomal protective protein (CTSA)					9.4
LDTP13282	Prenylcysteine oxidase 1 (PCYOX1)					9.4
LDTP13751	Exonuclease 1 (EXO1)					8.2
LDTP06375	Delta(24)-sterol reductase (DHCR24)					7.6
LDTP00832	NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3)					7.4
LDTP04004	Lysosomal Pro-X carboxypeptidase (PRCP)					7.2
LDTP04634	Alpha-N-acetylglucosaminidase (NAGLU)					7.0
LDTP06645	Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH)					6.9
LDTP09256	Putative phospholipase B-like 2 (PLBD2)					6.7
LDTP06200	Delta(14)-sterol reductase LBR (LBR)					6.3
LDTP04008	Endothelin-converting enzyme 1 (ECE1)					6.3
LDTP00697	Cytochrome b5 type B (CYB5B)					5.9
LDTP01039	Protein-S-isoprenylcysteine O-methyltransferase (ICMT)					5.8
LDTP05476	3-ketodihydrosphingosine reductase (KDSR)					5.7
LDTP12670	SET domain-containing protein 4 (SETD4)					5.7
LDTP09874	Endoplasmic reticulum protein SC65 (P3H4)					5.7
LDTP15422	Prostaglandin reductase 3 (PTGR3)					5.7
LDTP00338	Lysosomal alpha-mannosidase (MAN2B1)					5.6
LDTP10329	Ceramide synthase 2 (CERS2)					5.6
LDTP01389	Delta(14)-sterol reductase TM7SF2 (TM7SF2)					5.4
LDTP06147	ATP-dependent RNA helicase DHX8 (DHX8)					5.3
LDTP05408	Antigen peptide transporter 1 (TAP1)					5.2
LDTP08265	N-alpha-acetyltransferase 40 (NAA40)					5.2
LDTP02539	Lysosomal acid phosphatase (ACP2)					5.1
LDTP03417	Proteasome subunit beta type-4 (PSMB4)					5.1
LDTP03394	Ceramide synthase 1 (CERS1)					5.1

Target ID	Target Name					Binding Ratio
LDTP03132	Voltage-dependent anion-selective channel protein 1 (VDAC1)					16.2
LDTP03277	ER lumen protein-retaining receptor 1 (KDELR1)					12.0
LDTP15948	Transmembrane protein 126A (TMEM126A)					11.0
LDTP18153	Major facilitator superfamily domain-containing protein 3 (MFSD3)					10.6
LDTP04071	Voltage-dependent anion-selective channel protein 2 (VDAC2)					9.4
LDTP02215	Prosaposin (PSAP)					7.3
LDTP07236	Protein GPR107 (GPR107)					7.3
LDTP11615	Protein tweety homolog 3 (TTYH3)					7.0
LDTP12383	Protein GPR108 (GPR108)					6.9
LDTP09968	V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1)					6.5
LDTP02071	Amyloid-beta precursor protein (APP)					6.5
LDTP10958	Sigma non-opioid intracellular receptor 1 (SIGMAR1)					6.5
LDTP04033	Translocon-associated protein subunit alpha (SSR1)					6.4
LDTP09028	Mitoguardin 1 (MIGA1)					6.3
LDTP14511	ATP synthase subunit g, mitochondrial (ATP5MG)					6.3
LDTP02724	Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2)					5.9
LDTP14200	Mitochondrial ornithine transporter 1 (SLC25A15)					5.8
LDTP16069	Peroxisomal membrane protein 2 (PXMP2)					5.7
LDTP06221	StAR-related lipid transfer protein 3 (STARD3)					5.7
LDTP06426	Translocating chain-associated membrane protein 1 (TRAM1)					5.7
LDTP08881	Transmembrane protein 199 (TMEM199)					5.6
LDTP09547	Scavenger receptor class B member 1 (SCARB1)					5.4
LDTP10885	Sortilin (SORT1)					5.2
LDTP02010	Annexin A1 (ANXA1)					5.1

Target ID	Target Name					Binding Ratio
LDTP08594	Negative elongation factor C/D (NELFCD)					27.9
LDTP13311	Poly(U)-binding-splicing factor PUF60 (PUF60)					13.9
LDTP05160	Nucleobindin-2 (NUCB2)					13.5
LDTP11076	Apolipoprotein L2 (APOL2)					10.5
LDTP14486	Clustered mitochondria protein homolog (CLUH)					9.9
LDTP12565	Mitochondrial import receptor subunit TOM22 homolog (TOMM22)					9.8
LDTP00105	Protein unc-119 homolog B (UNC119B)					9.0
LDTP01451	UBX domain-containing protein 7 (UBXN7)					8.7
LDTP15067	Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6)					8.5
LDTP07368	Tetratricopeptide repeat protein 19, mitochondrial (TTC19)					8.2
LDTP00281	Golgi integral membrane protein 4 (GOLIM4)					8.1
LDTP19021	EF-hand calcium-binding domain-containing protein 14 (EFCAB14)					6.8
LDTP13606	Mammalian ependymin-related protein 1 (EPDR1)					6.2
LDTP14304	MAU2 chromatid cohesion factor homolog (MAU2)					6.2
LDTP02570	Ubiquitin-like protein 4A (UBL4A)					5.9
LDTP13828	Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3)					5.7
LDTP00417	Programmed cell death protein 5 (PDCD5)					5.4
LDTP14458	Tetraspanin-6 (TSPAN6)					5.3
LDTP13630	Neudesin (NENF)					5.2
LDTP15623	Neuferricin (CYB5D2)					5.2
LDTP13902	Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3)					5.1
LDTP04652	Eukaryotic translation initiation factor 5 (EIF5)					5.0
LDTP06097	Reticulocalbin-2 (RCN2)					5.0
LDTP18561	Small ribosomal subunit protein mS33 (MRPS33)					5.0
LDTP04395	RNA-binding protein FXR1 (FXR1)					5.0
LDTP07574	Superkiller complex protein 3 (SKIC3)					5.0

1	Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26. Mass spectrometry data entry: PXD041587