Details of the Target

General Information of Target

Target ID LDTP00575
Target Name Glutathione S-transferase A4 (GSTA4)
Gene Name GSTA4
Gene ID 2941
Synonyms
Glutathione S-transferase A4; EC 2.5.1.18; GST class-alpha member 4; Glutathione S-transferase A4-4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAARPKLHYPNGRGRMESVRWVLAAAGVEFDEEFLETKEQLYKLQDGNHLLFQQVPMVEI
DGMKLVQTRSILHYIADKHNLFGKNLKERTLIDMYVEGTLDLLELLIMHPFLKPDDQQKE
VVNMAQKAIIRYFPVFEKILRGHGQSFLVGNQLSLADVILLQTILALEEKIPNILSAFPF
LQEYTVKLSNIPTIKRFLEPGSKKKPPPDEIYVRTVYNIFRP
Target Bioclass
Enzyme
Family
GST superfamily, Alpha family
Subcellular location
Cytoplasm
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).
Uniprot ID
O15217
Ensemble ID
ENST00000370959.1
HGNC ID
HGNC:4629
ChEMBL ID
CHEMBL4933

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
IGROV1 SNV: p.L7R .
LS123 SNV: p.V122F .
LS180 SNV: p.L175M .
SKMEL2 SNV: p.V66A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y9(12.42)  LDD0257  [1]
TH214
 Probe Info  		Y9(20.00)  LDD0258  [1]
HHS-482
 Probe Info  		Y9(2.09)  LDD0285  [2]
ATP probe
 Probe Info  		N.A.  LDD0199  [3]
OSF
 Probe Info  		N.A.  LDD0029  [4]
SF
 Probe Info  		Y217(0.00); Y9(0.00)  LDD0028  [4]
PAL-AfBPP Probe
Click To Hide/Show 27 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C001
 Probe Info  		16.11  LDD1711  [5]
C004
 Probe Info  		29.86  LDD1714  [5]
C017
 Probe Info  		6.06  LDD1725  [5]
C055
 Probe Info  		30.91  LDD1752  [5]
C059
 Probe Info  		7.94  LDD1756  [5]
C064
 Probe Info  		8.06  LDD1761  [5]
C106
 Probe Info  		81.01  LDD1793  [5]
C108
 Probe Info  		32.22  LDD1795  [5]
C211
 Probe Info  		81.57  LDD1885  [5]
C212
 Probe Info  		5.43  LDD1886  [5]
C213
 Probe Info  		83.87  LDD1887  [5]
C214
 Probe Info  		8.22  LDD1888  [5]
C227
 Probe Info  		16.56  LDD1900  [5]
C228
 Probe Info  		16.11  LDD1901  [5]
C236
 Probe Info  		4.96  LDD1909  [5]
C238
 Probe Info  		20.82  LDD1911  [5]
C239
 Probe Info  		5.82  LDD1912  [5]
C240
 Probe Info  		22.94  LDD1913  [5]
C241
 Probe Info  		6.41  LDD1914  [5]
C274
 Probe Info  		7.94  LDD1944  [5]
C277
 Probe Info  		21.26  LDD1947  [5]
C307
 Probe Info  		5.62  LDD1975  [5]
C310
 Probe Info  		7.94  LDD1977  [5]
C342
 Probe Info  		34.54  LDD2004  [5]
C343
 Probe Info  		68.12  LDD2005  [5]
C344
 Probe Info  		18.64  LDD2006  [5]
C380
 Probe Info  		8.00  LDD2039  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0123  JWB131 DM93 Y9(2.09)  LDD0285  [2]
 LDCM0124  JWB142 DM93 Y9(1.01)  LDD0286  [2]
 LDCM0125  JWB146 DM93 Y9(4.21)  LDD0287  [2]
 LDCM0126  JWB150 DM93 Y9(5.00)  LDD0288  [2]
 LDCM0127  JWB152 DM93 Y9(3.77)  LDD0289  [2]
 LDCM0128  JWB198 DM93 Y9(20.00)  LDD0290  [2]
 LDCM0129  JWB202 DM93 Y9(16.61)  LDD0291  [2]
 LDCM0130  JWB211 DM93 Y9(4.53)  LDD0292  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glutathione S-transferase A2 (GSTA2) Alpha family P09210
Glutathione S-transferase A4 (GSTA4) Alpha family O15217
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Dynein regulatory complex subunit 4 (GAS8) DRC4 family O95995
U6 snRNA-associated Sm-like protein LSm1 (LSM1) SnRNP Sm proteins family O15116
Uncharacterized protein C2orf50 (C2orf50) . Q96LR7

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Glutathione Small molecular drug DB00143

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
4 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
5 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587