Details of the Probe

General Information of Probe

Probe ID
LDPC0448
Probe Name
C214
Synonyms
Z3337873097
Probe Type
PAL-AfBPP Probe
Structure
2D MOL
Ro5 Violations (Lipinski): 0 Molecular Weight (mw) 364.4
Lipid-water partition coefficient (xlogp) 1.9
Rotatable Bond Count (rotbonds) 9
Hydrogen Bond Donor Count (hbonddonor) 3
Hydrogen Bond Acceptor Count (hbondacc) 5
Chemical Identifiers
Formula
C19H20N6O2
IUPAC Name
3-(3-but-3-ynyldiazirin-3-yl)-N-[2-oxo-1-phenyl-2-(1H-pyrazol-5-ylamino)ethyl]propanamide
Canonical SMILES
C#CCCC1(N=N1)CCC(=O)NC(C2=CC=CC=C2)C(=O)NC3=CC=NN3
InChI
InChI=1S/C19H20N6O2/c1-2-3-11-19(24-25-19)12-9-16(26)22-17(14-7-5-4-6-8-14)18(27)21-15-10-13-20-23-15/h1,4-8,10,13,17H,3,9,11-12H2,(H,22,26)(H2,20,21,23,27)
InChIKey
YBPMLTLNTFAYIQ-UHFFFAOYSA-N

The Probe Interaction Atlas

Target(s) List of this Probe

28 Enzyme Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
ADP-dependent glucokinase (ADPGK) LDTP11127 5.50  LDD1888 
Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) LDTP03257 39.95  LDD1888 
CCR4-NOT transcription complex subunit 4 (CNOT4) LDTP01640 5.86  LDD1888 
Cytochrome b-c1 complex subunit 9 (UQCR10) LDTP13189 5.43  LDD1888 
Diacylglycerol lipase-beta (DAGLB) LDTP09095 7.57  LDD1888 
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (ALG11) LDTP06721 5.70  LDD1888 
Glutathione S-transferase A4 (GSTA4) LDTP00575 8.22  LDD1888 
Heme oxygenase 1 (HMOX1) LDTP02359 6.77  LDD1888 
Heme oxygenase 2 (HMOX2) LDTP03540 6.32  LDD1888 
L-2-hydroxyglutarate dehydrogenase, mitochondrial (L2HGDH) LDTP12105 9.06  LDD1888 
Lanosterol 14-alpha demethylase (CYP51A1) LDTP06648 11.31  LDD1888 
Lysophosphatidic acid phosphatase type 6 (ACP6) LDTP12363 10.63  LDD1888 
Myotubularin-related protein 12 (MTMR12) LDTP11617 5.66  LDD1888 
NADH dehydrogenase 1 alpha subcomplex subunit 7 (NDUFA7) LDTP01518 6.77  LDD1888 
NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) LDTP00832 10.13  LDD1888 
Peptidyl-prolyl cis-trans isomerase FKBP14 (FKBP14) LDTP12720 6.28  LDD1888 
Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) LDTP14218 48.17  LDD1888 
Prenylcysteine oxidase 1 (PCYOX1) LDTP13282 21.86  LDD1888 
Prenylcysteine oxidase-like (PCYOX1L) LDTP15480 6.45  LDD1888 
Prolyl 3-hydroxylase 1 (P3H1) LDTP06750 5.70  LDD1888 
Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) LDTP02679 5.74  LDD1888 
Prolyl 4-hydroxylase subunit alpha-2 (P4HA2) LDTP00645 6.87  LDD1888 
Prostaglandin reductase 3 (PTGR3) LDTP15422 76.11  LDD1888 
Proteasome subunit beta type-2 (PSMB2) LDTP04285 5.66  LDD1888 
Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) LDTP15484 19.97  LDD1888 
Serum paraoxonase/arylesterase 2 (PON2) LDTP06333 11.00  LDD1888 
tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase (FTSJ1) LDTP13200 5.50  LDD1888 
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1) LDTP06320 6.59  LDD1888 
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⏷ Show the Full List of 28 Enzyme
21 Transporter and channel Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Cell division cycle protein 20 homolog (CDC20) LDTP05662 21.26  LDD1888 
Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) LDTP02724 28.84  LDD1888 
ER lumen protein-retaining receptor 1 (KDELR1) LDTP03277 7.73  LDD1888 
Melanoma inhibitory activity protein 2 (MIA2) LDTP10669 5.98  LDD1888 
Metaxin-1 (MTX1) LDTP05900 5.62  LDD1888 
Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) LDTP07885 20.39  LDD1888 
Phosphatidylinositol transfer protein alpha isoform (PITPNA) LDTP05236 6.36  LDD1888 
Protein FAM8A1 (FAM8A1) LDTP16190 10.93  LDD1888 
Protein sel-1 homolog 1 (SEL1L) LDTP13164 8.82  LDD1888 
Putative divalent cation/proton antiporter TMEM165 (TMEM165) LDTP12207 6.73  LDD1888 
Signal recognition particle 9 kDa protein (SRP9) LDTP04259 6.68  LDD1888 
Solute carrier family 25 member 32 (SLC25A32) LDTP11813 11.55  LDD1888 
Sorting nexin-2 (SNX2) LDTP01043 7.84  LDD1888 
SUN domain-containing protein 2 (SUN2) LDTP13256 12.91  LDD1888 
Surfeit locus protein 4 (SURF4) LDTP00592 7.67  LDD1888 
Synaptojanin-2-binding protein (SYNJ2BP) LDTP04789 8.46  LDD1888 
Translocating chain-associated membrane protein 1 (TRAM1) LDTP06426 6.59  LDD1888 
Translocon-associated protein subunit alpha (SSR1) LDTP04033 6.45  LDD1888 
Transmembrane protein 126A (TMEM126A) LDTP15948 7.57  LDD1888 
Transmembrane protein 160 (TMEM160) LDTP20002 8.06  LDD1888 
Transmembrane protein 199 (TMEM199) LDTP08881 6.63  LDD1888 
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⏷ Show the Full List of 21 Transporter and channel
1 Transcription factor Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Y-box-binding protein 1 (YBX1) LDTP05065 6.54  LDD1888 
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31 Other Labeled by This Probe
Target Name Target ID Binding Site(Ratio) Interaction ID
Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) LDTP08364 6.15  LDD1888 
Apolipoprotein L2 (APOL2) LDTP11076 11.24  LDD1888 
Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) LDTP13755 9.85  LDD1888 
Calmodulin-3 (CALM3) LDTP02443 5.90  LDD1888 
Calumenin (CALU) LDTP00887 8.00  LDD1888 
Centrosomal protein 43 (CEP43) LDTP01652 6.45  LDD1888 
Clustered mitochondria protein homolog (CLUH) LDTP14486 8.17  LDD1888 
Cytoplasmic dynein 2 intermediate chain 2 (DYNC2I2) LDTP10281 6.54  LDD1888 
DnaJ homolog subfamily B member 11 (DNAJB11) LDTP13152 6.82  LDD1888 
Dynactin subunit 3 (DCTN3) LDTP01359 5.58  LDD1888 
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) LDTP13828 11.96  LDD1888 
ER lumen protein-retaining receptor 3 (KDELR3) LDTP00850 8.69  LDD1888 
Eukaryotic translation initiation factor 3 subunit H (EIF3H) LDTP00620 8.51  LDD1888 
Golgi reassembly-stacking protein 2 (GORASP2) LDTP12073 5.39  LDD1888 
Mitochondrial import receptor subunit TOM22 homolog (TOMM22) LDTP12565 8.40  LDD1888 
Neudesin (NENF) LDTP13630 6.15  LDD1888 
Neuferricin (CYB5D2) LDTP15623 5.94  LDD1888 
Neurofilament medium polypeptide (NEFM) LDTP02181 5.62  LDD1888 
Nucleobindin-1 (NUCB1) LDTP05375 7.01  LDD1888 
Nucleobindin-2 (NUCB2) LDTP05160 11.88  LDD1888 
Protein CEBPZOS (CEBPZOS) LDTP18883 5.90  LDD1888 
Protein FAM162A (FAM162A) LDTP10056 5.54  LDD1888 
Protein SET (SET) LDTP05277 5.94  LDD1888 
Protein YIF1A (YIF1A) LDTP01494 6.15  LDD1888 
Rap guanine nucleotide exchange factor 2 (RAPGEF2) LDTP14058 5.39  LDD1888 
RNA polymerase II-associated protein 1 (RPAP1) LDTP11327 6.82  LDD1888 
RNA-binding protein 3 (RBM3) LDTP05226 6.63  LDD1888 
TATA-binding protein-associated factor 2N (TAF15) LDTP09881 5.66  LDD1888 
Thioredoxin-related transmembrane protein 4 (TMX4) LDTP18373 6.11  LDD1888 
Ubiquitin-associated protein 2 (UBAP2) LDTP07132 6.32  LDD1888 
WD repeat and coiled-coil-containing protein (WDCP) LDTP11995 8.46  LDD1888 
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⏷ Show the Full List of 31 Other

Full Information of The Labelling Profiles of This Probe

Quantification: AfBPP Probe vs Negative Probe
  
Experiment 1 Reporting the Labelling Profiles of This Probe
REF [1]
Probe concentration
Probe:50uM; negative probe:50uM
Quantitative Method
TMT
In Vitro Experiment Model
Model Type
Living cell
Derived Tissue
Peripheral blood
Disease Model
Normal
Model Name
Human normal cells (HEK-293T)
Note
Negative probe:C#CCCC1(CCC(NC)=O)N=N1
Interaction Atlas ID
 LDD1888 
Download The Altas

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587