Details of the Target

General Information of Target

Target ID LDTP05226
Target Name RNA-binding protein 3 (RBM3)
Gene Name RBM3
Gene ID 5935
Synonyms
RNPL; RNA-binding protein 3; RNA-binding motif protein 3; RNPL
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSSEEGKLFVGGLNFNTDEQALEDHFSSFGPISEVVVVKDRETQRSRGFGFITFTNPEHA
SVAMRAMNGESLDGRQIRVDHAGKSARGTRGGGFGAHGRGRSYSRGGGDQGYGSGRYYDS
RPGGYGYGYGRSRDYNGRNQGGYDRYSGGNYRDNYDN
Target Bioclass
Other
Subcellular location
Nucleus
Function
Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.
Uniprot ID
P98179
Ensemble ID
ENST00000376755.1
HGNC ID
HGNC:9900
ChEMBL ID
CHEMBL4295793

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ONAyne
 Probe Info  		K84(6.67)  LDD0274  [1]
STPyne
 Probe Info  		K84(10.00)  LDD0277  [1]
AZ-9
 Probe Info  		E70(10.00)  LDD2208  [2]
HHS-482
 Probe Info  		Y125(0.94); Y127(0.91)  LDD0285  [3]
HHS-475
 Probe Info  		Y125(0.81); Y127(0.88); Y118(0.93); Y117(0.94)  LDD0264  [4]
Acrolein
 Probe Info  		N.A.  LDD0221  [5]
5E-2FA
 Probe Info  		N.A.  LDD2235  [6]
m-APA
 Probe Info  		N.A.  LDD2231  [6]
SF
 Probe Info  		Y155(0.00); Y125(0.00); Y117(0.00); Y127(0.00)  LDD0028  [7]
1c-yne
 Probe Info  		N.A.  LDD0228  [8]
HHS-465
 Probe Info  		Y125(0.00); Y127(0.00); Y117(0.00); Y118(0.00)  LDD2240  [9]
PAL-AfBPP Probe
Click To Hide/Show 49 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C131
 Probe Info  		5.17  LDD1813  [10]
C139
 Probe Info  		11.08  LDD1821  [10]
C143
 Probe Info  		12.82  LDD1825  [10]
C145
 Probe Info  		14.03  LDD1827  [10]
C147
 Probe Info  		7.84  LDD1829  [10]
C165
 Probe Info  		14.62  LDD1845  [10]
C173
 Probe Info  		5.94  LDD1853  [10]
C178
 Probe Info  		16.56  LDD1857  [10]
C187
 Probe Info  		17.51  LDD1865  [10]
C191
 Probe Info  		13.00  LDD1868  [10]
C206
 Probe Info  		15.78  LDD1881  [10]
C210
 Probe Info  		54.57  LDD1884  [10]
C211
 Probe Info  		9.65  LDD1885  [10]
C212
 Probe Info  		5.66  LDD1886  [10]
C213
 Probe Info  		19.84  LDD1887  [10]
C214
 Probe Info  		6.63  LDD1888  [10]
C232
 Probe Info  		55.33  LDD1905  [10]
C235
 Probe Info  		17.63  LDD1908  [10]
C249
 Probe Info  		13.36  LDD1922  [10]
C280
 Probe Info  		10.13  LDD1950  [10]
C282
 Probe Info  		14.32  LDD1952  [10]
C284
 Probe Info  		34.78  LDD1954  [10]
C305
 Probe Info  		12.30  LDD1974  [10]
C313
 Probe Info  		24.76  LDD1980  [10]
C314
 Probe Info  		17.03  LDD1981  [10]
C326
 Probe Info  		7.78  LDD1990  [10]
C346
 Probe Info  		16.22  LDD2007  [10]
C347
 Probe Info  		6.50  LDD2008  [10]
C348
 Probe Info  		12.30  LDD2009  [10]
C350
 Probe Info  		26.72  LDD2011  [10]
C353
 Probe Info  		8.69  LDD2014  [10]
C354
 Probe Info  		9.38  LDD2015  [10]
C355
 Probe Info  		36.50  LDD2016  [10]
C356
 Probe Info  		8.94  LDD2017  [10]
C361
 Probe Info  		32.00  LDD2022  [10]
C363
 Probe Info  		20.68  LDD2024  [10]
C364
 Probe Info  		17.51  LDD2025  [10]
C390
 Probe Info  		40.22  LDD2049  [10]
C391
 Probe Info  		31.34  LDD2050  [10]
C392
 Probe Info  		6.32  LDD2051  [10]
C393
 Probe Info  		5.17  LDD2052  [10]
C394
 Probe Info  		4.99  LDD2053  [10]
C403
 Probe Info  		16.91  LDD2061  [10]
C420
 Probe Info  		10.48  LDD2075  [10]
FFF probe11
 Probe Info  		8.94  LDD0471  [11]
FFF probe13
 Probe Info  		14.80  LDD0475  [11]
FFF probe2
 Probe Info  		18.52  LDD0463  [11]
FFF probe3
 Probe Info  		12.37  LDD0464  [11]
OEA-DA
 Probe Info  		4.04  LDD0046  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0116  HHS-0101 DM93 Y125(0.81); Y127(0.88); Y118(0.93); Y117(0.94)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y127(0.39); Y117(0.81); Y118(0.86); Y125(0.87)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y143(0.35); Y117(0.54); Y118(0.59); Y125(0.77)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y127(0.62); Y125(0.80); Y118(0.84); Y117(0.86)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y117(0.82); Y127(0.86); Y118(0.88); Y125(0.94)  LDD0268  [4]
 LDCM0107  IAA HeLa N.A.  LDD0221  [5]
 LDCM0123  JWB131 DM93 Y125(0.94); Y127(0.91)  LDD0285  [3]
 LDCM0124  JWB142 DM93 Y125(0.57); Y127(0.54)  LDD0286  [3]
 LDCM0125  JWB146 DM93 Y125(1.11); Y127(1.19)  LDD0287  [3]
 LDCM0126  JWB150 DM93 Y125(2.40); Y127(2.61)  LDD0288  [3]
 LDCM0127  JWB152 DM93 Y125(2.07); Y127(1.89)  LDD0289  [3]
 LDCM0128  JWB198 DM93 Y125(1.00); Y127(1.01)  LDD0290  [3]
 LDCM0129  JWB202 DM93 Y125(0.28); Y127(0.32)  LDD0291  [3]
 LDCM0130  JWB211 DM93 Y125(1.05); Y127(0.97)  LDD0292  [3]
 LDCM0109  NEM HeLa N.A.  LDD0223  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Myb-related transcription factor, partner of profilin (MYPOP) . Q86VE0
Other
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
KH domain-containing, RNA-binding, signal transduction-associated protein 2 (KHDRBS2) KHDRBS family Q5VWX1
KH domain-containing, RNA-binding, signal transduction-associated protein 3 (KHDRBS3) KHDRBS family O75525
Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) RRM IMP/VICKZ family Q9Y6M1
U1 small nuclear ribonucleoprotein A (SNRPA) RRM U1 A/B'' family P09012
Serine/arginine-rich splicing factor 3 (SRSF3) Splicing factor SR family P84103
Heterogeneous nuclear ribonucleoprotein K (HNRNPK) . P61978
Poly(rC)-binding protein 1 (PCBP1) . Q15365
Proline-rich protein 3 (PRR3) . P79522
RNA-binding motif protein, X chromosome (RBMX) . P38159
RNA-binding motif protein, Y chromosome, family 1 member A1 (RBMY1A1) . P0DJD3
RNA-binding motif protein, Y chromosome, family 1 member F/J (RBMY1F; RBMY1J) . Q15415
RNA-binding protein with multiple splicing 2 (RBPMS2) . Q6ZRY4

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
8 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
9 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
11 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
12 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570