Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C354  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 78 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP06150  DNA replication licensing factor MCM6 (MCM6) 72.0
 LDTP03540  Heme oxygenase 2 (HMOX2) 56.9
 LDTP03842  Squalene synthase (FDFT1) 37.3
 LDTP04004  Lysosomal Pro-X carboxypeptidase (PRCP) 35.8
 LDTP03189  Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) 28.2
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 25.1
 LDTP05758  S-methyl-5'-thioadenosine phosphorylase (MTAP) 25.1
 LDTP01773  Cytochrome c oxidase subunit 2 (MT-CO2) 24.9
 LDTP15484  Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) 23.9
 LDTP09088  Phospholipase A2 group XV (PLA2G15) 22.6
 LDTP01743  NADH dehydrogenase 1 beta subcomplex subunit 10 (NDUFB10) 21.1
 LDTP03257  Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) 20.4
 LDTP16032  Retinoid-inducible serine carboxypeptidase (SCPEP1) 18.3
 LDTP16007  Lipid droplet-associated hydrolase (LDAH) 17.5
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 16.6
 LDTP01803  Adenosine deaminase (ADA) 16.4
 LDTP12363  Lysophosphatidic acid phosphatase type 6 (ACP6) 16.3
 LDTP00697  Cytochrome b5 type B (CYB5B) 16.2
 LDTP03417  Proteasome subunit beta type-4 (PSMB4) 15.3
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 15.1
 LDTP03223  Small ribosomal subunit protein uS3 (RPS3) 14.8
 LDTP01769  Dihydrofolate reductase (DHFR) 13.9
 LDTP08859  Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4) 13.9
 LDTP09256  Putative phospholipase B-like 2 (PLBD2) 13.9
 LDTP10329  Ceramide synthase 2 (CERS2) 13.5
 LDTP00783  Beta-1,4-glucuronyltransferase 1 (B4GAT1) 12.9
 LDTP12720  Peptidyl-prolyl cis-trans isomerase FKBP14 (FKBP14) 12.6
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 12.4
 LDTP01419  Retinal dehydrogenase 2 (ALDH1A2) 12.0
 LDTP10188  FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) 11.7
 LDTP04925  Pterin-4-alpha-carbinolamine dehydratase (PCBD1) 11.6
 LDTP13358  Leucyl-cystinyl aminopeptidase (LNPEP) 11.6
 LDTP03418  Proteasome subunit beta type-6 (PSMB6) 11.6
 LDTP03560  Aldehyde dehydrogenase X, mitochondrial (ALDH1B1) 11.5
 LDTP03001  E3 ubiquitin-protein ligase TRIM21 (TRIM21) 11.5
 LDTP04008  Endothelin-converting enzyme 1 (ECE1) 11.2
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 10.9
 LDTP09799  DCN1-like protein 4 (DCUN1D4) 10.7
 LDTP08979  Prostaglandin reductase 2 (PTGR2) 10.2
 LDTP04634  Alpha-N-acetylglucosaminidase (NAGLU) 10.1
 LDTP11321  Acetyl-CoA acetyltransferase, cytosolic (ACAT2) 10.0
 LDTP01687  Apoptosis-inducing factor 1, mitochondrial (AIFM1) 9.8
 LDTP01982  NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) 9.8
 LDTP02359  Heme oxygenase 1 (HMOX1) 9.4
 LDTP02254  ATP-dependent translocase ABCB1 (ABCB1) 9.4
 LDTP13189  Cytochrome b-c1 complex subunit 9 (UQCR10) 9.4
 LDTP12868  Endoplasmic reticulum aminopeptidase 1 (ERAP1) 9.3
 LDTP05029  Peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A) 9.3
 LDTP00988  Protein O-GlcNAcase (OGA) 8.9
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 8.6
 LDTP08476  5'-3' exonuclease PLD3 (PLD3) 8.6
 LDTP03198  Ferrochelatase, mitochondrial (FECH) 8.6
 LDTP06333  Serum paraoxonase/arylesterase 2 (PON2) 8.5
 LDTP00486  Cytochrome b-c1 complex subunit 8 (UQCRQ) 8.4
 LDTP03608  RAC-beta serine/threonine-protein kinase (AKT2) 8.3
 LDTP10249  Metalloendopeptidase OMA1, mitochondrial (OMA1) 8.3
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 8.3
 LDTP10862  Proteasome subunit beta type-7 (PSMB7) 8.3
 LDTP00907  Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta (PDE6D) 8.3
 LDTP01168  NADH dehydrogenase iron-sulfur protein 2, mitochondrial (NDUFS2) 8.2
 LDTP06315  Platelet-activating factor acetylhydrolase IB subunit alpha1 (PAFAH1B3) 8.2
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 8.2
 LDTP06849  Prolyl endopeptidase-like (PREPL) 8.1
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 8.0
 LDTP19838  Immunity-related GTPase family Q protein (IRGQ) 8.0
 LDTP12959  NADH dehydrogenase 1 alpha subcomplex subunit 13 (NDUFA13) 7.9
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 7.7
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 7.5
 LDTP02169  Beta-hexosaminidase subunit alpha (HEXA) 7.4
 LDTP06477  Methylsterol monooxygenase 1 (MSMO1) 7.4
 LDTP03697  Mannose-6-phosphate isomerase (MPI) 7.2
 LDTP06023  Calcium/calmodulin-dependent protein kinase type 1 (CAMK1) 7.2
 LDTP19851  Isochorismatase domain-containing protein 1 (ISOC1) 7.2
 LDTP09874  Endoplasmic reticulum protein SC65 (P3H4) 7.1
 LDTP05918  Histone deacetylase 1 (HDAC1) 7.1
 LDTP01300  Cartilage-associated protein (CRTAP) 7.0
 LDTP02799  N-acetylglucosamine-6-sulfatase (GNS) 7.0
 LDTP11437  Peroxisomal trans-2-enoyl-CoA reductase (PECR) 7.0
Transporter and channel
Click to Show/Hide to Show 40 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01804  ATP synthase subunit a (MT-ATP6) 58.1
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 52.7
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 44.6
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 22.8
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 21.7
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 18.6
 LDTP00865  Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) 18.5
 LDTP07236  Protein GPR107 (GPR107) 18.3
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 17.5
 LDTP08881  Transmembrane protein 199 (TMEM199) 15.8
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 15.7
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 14.8
 LDTP12207  Putative divalent cation/proton antiporter TMEM165 (TMEM165) 14.0
 LDTP17641  Solute carrier family 35 member F2 (SLC35F2) 13.8
 LDTP10958  Sigma non-opioid intracellular receptor 1 (SIGMAR1) 13.3
 LDTP15948  Transmembrane protein 126A (TMEM126A) 13.1
 LDTP01217  Metaxin-2 (MTX2) 12.0
 LDTP07462  MFS-type transporter SLC18B1 (SLC18B1) 12.0
 LDTP11245  Derlin-1 (DERL1) 11.8
 LDTP03546  Translocator protein (TSPO) 11.8
 LDTP05900  Metaxin-1 (MTX1) 11.1
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 10.4
 LDTP04592  Monocarboxylate transporter 1 (SLC16A1) 10.3
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 10.1
 LDTP14099  Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) 10.1
 LDTP15481  Transmembrane protein 87A (TMEM87A) 10.1
 LDTP11615  Protein tweety homolog 3 (TTYH3) 9.8
 LDTP10731  Sodium-coupled neutral amino acid symporter 2 (SLC38A2) 9.3
 LDTP03559  High affinity cationic amino acid transporter 1 (SLC7A1) 8.8
 LDTP16813  Tetraspanin-3 (TSPAN3) 8.6
 LDTP12383  Protein GPR108 (GPR108) 8.5
 LDTP09968  V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) 8.3
 LDTP00592  Surfeit locus protein 4 (SURF4) 7.9
 LDTP05511  Cytoskeleton-associated protein 4 (CKAP4) 7.6
 LDTP01366  Flotillin-1 (FLOT1) 7.6
 LDTP18459  StAR-related lipid transfer protein 7, mitochondrial (STARD7) 7.4
 LDTP14200  Mitochondrial ornithine transporter 1 (SLC25A15) 7.2
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 7.1
 LDTP09987  Secretory carrier-associated membrane protein 4 (SCAMP4) 7.0
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 7.0
Transcription factor
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP05065  Y-box-binding protein 1 (YBX1) 7.7
Immunoglobulin
Click to Show/Hide to Show 3 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP04720  IgG receptor FcRn large subunit p51 (FCGRT) 12.6
 LDTP03772  Basigin (BSG) 8.4
 LDTP02040  HLA class I histocompatibility antigen, A alpha chain (HLA-A) 7.9
Other
Click to Show/Hide to Show 70 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP15269  Large ribosomal subunit protein mL55 (MRPL55) 99.7
 LDTP02345  Retinol-binding protein 1 (RBP1) 99.7
 LDTP13630  Neudesin (NENF) 57.3
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 29.9
 LDTP15623  Neuferricin (CYB5D2) 28.4
 LDTP19372  Tetratricopeptide repeat protein 38 (TTC38) 26.2
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 25.6
 LDTP00850  ER lumen protein-retaining receptor 3 (KDELR3) 24.1
 LDTP05160  Nucleobindin-2 (NUCB2) 23.1
 LDTP14458  Tetraspanin-6 (TSPAN6) 21.3
 LDTP00871  Microtubule nucleation factor SSNA1 (SSNA1) 19.7
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 18.5
 LDTP06390  Protein transport protein Sec23A (SEC23A) 16.3
 LDTP11076  Apolipoprotein L2 (APOL2) 15.6
 LDTP06097  Reticulocalbin-2 (RCN2) 15.2
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 14.3
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 13.5
 LDTP16193  Craniofacial development protein 1 (CFDP1) 12.2
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 12.0
 LDTP13125  Protein UXT (UXT) 10.7
 LDTP00986  Syntaxin-10 (STX10) 10.5
 LDTP01652  Centrosomal protein 43 (CEP43) 10.3
 LDTP02124  Keratin, type I cytoskeletal 18 (KRT18) 10.3
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 10.2
 LDTP04094  Large proline-rich protein BAG6 (BAG6) 10.2
 LDTP05277  Protein SET (SET) 10.2
 LDTP02351  Tropomyosin alpha-1 chain (TPM1) 10.2
 LDTP12395  Large ribosomal subunit protein mL40 (MRPL40) 9.7
 LDTP15714  Mdm2-binding protein (MTBP) 9.7
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 9.4
 LDTP05226  RNA-binding protein 3 (RBM3) 9.4
 LDTP10965  Nucleosome assembly protein 1-like 4 (NAP1L4) 9.3
 LDTP09410  Protein bicaudal D homolog 2 (BICD2) 9.3
 LDTP07031  Collectin-12 (COLEC12) 9.2
 LDTP16080  Protein FAM114A2 (FAM114A2) 9.1
 LDTP00573  Prefoldin subunit 6 (PFDN6) 9.1
 LDTP00632  Syntaxin-7 (STX7) 9.1
 LDTP04684  Nucleosome assembly protein 1-like 1 (NAP1L1) 9.0
 LDTP01359  Dynactin subunit 3 (DCTN3) 8.9
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 8.9
 LDTP15420  Mitochondrial import receptor subunit TOM5 homolog (TOMM5) 8.9
 LDTP04652  Eukaryotic translation initiation factor 5 (EIF5) 8.8
 LDTP11978  HAUS augmin-like complex subunit 4 (HAUS4) 8.8
 LDTP11764  Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1) 8.6
 LDTP04111  Small ribosomal subunit protein eS10 (RPS10) 8.6
 LDTP00887  Calumenin (CALU) 8.5
 LDTP05375  Nucleobindin-1 (NUCB1) 8.4
 LDTP09069  Kinetochore protein Spc24 (SPC24) 8.3
 LDTP10056  Protein FAM162A (FAM162A) 8.3
 LDTP03775  RNA-binding protein FUS (FUS) 8.1
 LDTP12468  Diablo IAP-binding mitochondrial protein (DIABLO) 8.1
 LDTP02297  Vimentin (VIM) 8.1
 LDTP07368  Tetratricopeptide repeat protein 19, mitochondrial (TTC19) 7.9
 LDTP09920  Golgi apparatus protein 1 (GLG1) 7.9
 LDTP06022  Cold-inducible RNA-binding protein (CIRBP) 7.7
 LDTP11044  Polyadenylate-binding protein-interacting protein 2 (PAIP2) 7.7
 LDTP02869  Stathmin (STMN1) 7.6
 LDTP04595  Activated RNA polymerase II transcriptional coactivator p15 (SUB1) 7.6
 LDTP13311  Poly(U)-binding-splicing factor PUF60 (PUF60) 7.6
 LDTP13519  Proteasome activator complex subunit 2 (PSME2) 7.5
 LDTP01167  Reticulon-2 (RTN2) 7.5
 LDTP06527  Alpha-internexin (INA) 7.5
 LDTP09850  Proline-rich protein PRCC (PRCC) 7.4
 LDTP12642  CYFIP-related Rac1 interactor B (CYRIB) 7.3
 LDTP00913  Mitochondrial import inner membrane translocase subunit Tim8 A (TIMM8A) 7.3
 LDTP01075  Protein CutA (CUTA) 7.2
 LDTP04241  Nuclear autoantigenic sperm protein (NASP) 7.2
 LDTP10775  Endoplasmic reticulum-Golgi intermediate compartment protein 2 (ERGIC2) 7.1
 LDTP13309  Prefoldin subunit 2 (PFDN2) 7.0
 LDTP02165  Tropomyosin alpha-3 chain (TPM3) 7.0

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587