Details of the Target

General Information of Target

Target ID LDTP01419
Target Name Retinal dehydrogenase 2 (ALDH1A2)
Gene Name ALDH1A2
Gene ID 8854
Synonyms
RALDH2; Retinal dehydrogenase 2; RALDH 2; RalDH2; EC 1.2.1.36; Aldehyde dehydrogenase family 1 member A2; ALDH1A2; Retinaldehyde-specific dehydrogenase type 2; RALDH(II)
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTSSKIEMPGEVKADPAALMASLHLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVYNP
ATGEQVCEVQEADKADIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRAVL
ATMESLNGGKPFLQAFYVDLQGVIKTFRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVC
GQIIPWNFPLLMFAWKIAPALCCGNTVVIKPAEQTPLSALYMGALIKEAGFPPGVINILP
GYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNLKRVTLELGGKSPNIIFADAD
LDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVRRSVERAKRRVVGSPFDPTTEQG
PQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFG
PVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTVWINCYNALNAQ
SPFGGFKMSGNGREMGEFGLREYSEVKTVTVKIPQKNS
Target Bioclass
Enzyme
Family
Aldehyde dehydrogenase family
Subcellular location
Cytoplasm
Function
Catalyzes the NAD-dependent oxidation of aldehyde substrates, such as all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively. Retinoate signaling is critical for the transcriptional control of many genes, for instance it is crucial for initiation of meiosis in both male and female (Probable). Recognizes retinal as substrate, both in its free form and when bound to cellular retinol-binding protein. Can metabolize octanal and decanal, but has only very low activity with benzaldehyde, acetaldehyde and propanal. Displays complete lack of activity with citral.
Uniprot ID
O94788
Ensemble ID
ENST00000249750.9
HGNC ID
HGNC:15472
ChEMBL ID
CHEMBL3112384

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DAOY SNV: p.A151T .
DU145 Substitution: p.Q362K .
HT115 SNV: p.R148Ter .
KMCH1 SNV: p.G52W; p.P361T .
KMS11 SNV: p.S51R .
KYSE410 Deletion: p.L426Ter .
LS123 SNV: p.G263V .
MEWO SNV: p.P185S .
WM793 SNV: p.S125F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
A-EBA
 Probe Info  		4.08  LDD0215  [1]
YN-4
 Probe Info  		100.00  LDD0445  [2]
AZ-9
 Probe Info  		E286(1.00)  LDD2208  [3]
P11
 Probe Info  		20.00  LDD0201  [4]
Alkylaryl probe 3
 Probe Info  		3.13  LDD0282  [5]
Johansson_61
 Probe Info  		_(10.81)  LDD1487  [6]
DBIA
 Probe Info  		C387(8.30)  LDD0209  [7]
IA-alkyne
 Probe Info  		C202(0.00); C67(0.00); C180(0.00)  LDD0165  [8]
IPM
 Probe Info  		N.A.  LDD0005  [9]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [10]
1c-yne
 Probe Info  		N.A.  LDD0228  [11]
1d-yne
 Probe Info  		N.A.  LDD0357  [11]
AOyne
 Probe Info  		14.60  LDD0443  [12]
PAL-AfBPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C024
 Probe Info  		5.46  LDD1730  [13]
C138
 Probe Info  		5.58  LDD1820  [13]
C145
 Probe Info  		10.34  LDD1827  [13]
C147
 Probe Info  		11.24  LDD1829  [13]
C234
 Probe Info  		5.58  LDD1907  [13]
C347
 Probe Info  		5.03  LDD2008  [13]
C353
 Probe Info  		15.24  LDD2014  [13]
C354
 Probe Info  		12.04  LDD2015  [13]
C355
 Probe Info  		22.63  LDD2016  [13]
C361
 Probe Info  		32.90  LDD2022  [13]
C430
 Probe Info  		9.99  LDD2085  [13]
C432
 Probe Info  		8.94  LDD2087  [13]
C433
 Probe Info  		8.51  LDD2088  [13]
STS-1
 Probe Info  		N.A.  LDD0137  [14]
AEA-DA
 Probe Info  		3.29  LDD0333  [15]
OEA-DA
 Probe Info  		20.00  LDD0046  [15]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C320(1.09)  LDD1507  [16]
 LDCM0215  AC10 HEK-293T C320(1.02)  LDD1508  [16]
 LDCM0226  AC11 HEK-293T C320(1.11)  LDD1509  [16]
 LDCM0237  AC12 HEK-293T C320(1.08)  LDD1510  [16]
 LDCM0259  AC14 HEK-293T C320(0.95)  LDD1512  [16]
 LDCM0270  AC15 HEK-293T C320(0.86)  LDD1513  [16]
 LDCM0276  AC17 HEK-293T C320(1.10)  LDD1515  [16]
 LDCM0277  AC18 HEK-293T C320(1.34)  LDD1516  [16]
 LDCM0278  AC19 HEK-293T C320(1.02)  LDD1517  [16]
 LDCM0279  AC2 HEK-293T C320(0.92)  LDD1518  [16]
 LDCM0280  AC20 HEK-293T C320(0.93)  LDD1519  [16]
 LDCM0281  AC21 HEK-293T C473(1.03); C320(0.90)  LDD1520  [16]
 LDCM0282  AC22 HEK-293T C320(0.91)  LDD1521  [16]
 LDCM0283  AC23 HEK-293T C320(1.04)  LDD1522  [16]
 LDCM0284  AC24 HEK-293T C67(1.25)  LDD1523  [16]
 LDCM0285  AC25 HEK-293T C320(1.07)  LDD1524  [16]
 LDCM0286  AC26 HEK-293T C320(1.07)  LDD1525  [16]
 LDCM0287  AC27 HEK-293T C320(1.17)  LDD1526  [16]
 LDCM0288  AC28 HEK-293T C320(1.41)  LDD1527  [16]
 LDCM0289  AC29 HEK-293T C473(0.98); C320(0.96)  LDD1528  [16]
 LDCM0290  AC3 HEK-293T C320(1.36)  LDD1529  [16]
 LDCM0291  AC30 HEK-293T C320(1.10)  LDD1530  [16]
 LDCM0292  AC31 HEK-293T C320(0.99)  LDD1531  [16]
 LDCM0293  AC32 HEK-293T C67(1.11)  LDD1532  [16]
 LDCM0294  AC33 HEK-293T C320(0.96)  LDD1533  [16]
 LDCM0295  AC34 HEK-293T C320(0.99)  LDD1534  [16]
 LDCM0296  AC35 HEK-293T C320(1.33)  LDD1535  [16]
 LDCM0297  AC36 HEK-293T C320(0.86)  LDD1536  [16]
 LDCM0298  AC37 HEK-293T C473(0.86); C320(1.05)  LDD1537  [16]
 LDCM0299  AC38 HEK-293T C320(1.07)  LDD1538  [16]
 LDCM0300  AC39 HEK-293T C320(1.07)  LDD1539  [16]
 LDCM0301  AC4 HEK-293T C320(0.89)  LDD1540  [16]
 LDCM0302  AC40 HEK-293T C67(1.08)  LDD1541  [16]
 LDCM0303  AC41 HEK-293T C320(1.17)  LDD1542  [16]
 LDCM0304  AC42 HEK-293T C320(1.07)  LDD1543  [16]
 LDCM0305  AC43 HEK-293T C320(1.12)  LDD1544  [16]
 LDCM0306  AC44 HEK-293T C320(1.06)  LDD1545  [16]
 LDCM0307  AC45 HEK-293T C473(0.86); C320(0.97)  LDD1546  [16]
 LDCM0308  AC46 HEK-293T C320(1.32)  LDD1547  [16]
 LDCM0309  AC47 HEK-293T C320(0.84)  LDD1548  [16]
 LDCM0310  AC48 HEK-293T C67(1.05)  LDD1549  [16]
 LDCM0311  AC49 HEK-293T C320(1.07)  LDD1550  [16]
 LDCM0312  AC5 HEK-293T C473(0.75); C320(0.87)  LDD1551  [16]
 LDCM0313  AC50 HEK-293T C320(1.01)  LDD1552  [16]
 LDCM0314  AC51 HEK-293T C320(1.54)  LDD1553  [16]
 LDCM0315  AC52 HEK-293T C320(0.86)  LDD1554  [16]
 LDCM0316  AC53 HEK-293T C473(0.81); C320(0.92)  LDD1555  [16]
 LDCM0317  AC54 HEK-293T C320(0.96)  LDD1556  [16]
 LDCM0318  AC55 HEK-293T C320(1.10)  LDD1557  [16]
 LDCM0319  AC56 HEK-293T C67(1.04)  LDD1558  [16]
 LDCM0320  AC57 HEK-293T C320(1.10)  LDD1559  [16]
 LDCM0321  AC58 HEK-293T C320(0.94)  LDD1560  [16]
 LDCM0322  AC59 HEK-293T C320(1.58)  LDD1561  [16]
 LDCM0323  AC6 HEK-293T C320(1.31)  LDD1562  [16]
 LDCM0324  AC60 HEK-293T C320(1.31)  LDD1563  [16]
 LDCM0325  AC61 HEK-293T C473(0.99); C320(0.88)  LDD1564  [16]
 LDCM0326  AC62 HEK-293T C320(1.05)  LDD1565  [16]
 LDCM0327  AC63 HEK-293T C320(1.22)  LDD1566  [16]
 LDCM0328  AC64 HEK-293T C67(1.01)  LDD1567  [16]
 LDCM0334  AC7 HEK-293T C320(0.86)  LDD1568  [16]
 LDCM0345  AC8 HEK-293T C67(1.18)  LDD1569  [16]
 LDCM0248  AKOS034007472 HEK-293T C473(0.93); C320(0.96)  LDD1511  [16]
 LDCM0356  AKOS034007680 HEK-293T C320(1.19)  LDD1570  [16]
 LDCM0275  AKOS034007705 HEK-293T C67(1.03)  LDD1514  [16]
 LDCM0088  C45 HEK-293T 20.00  LDD0201  [4]
 LDCM0368  CL10 HEK-293T C320(2.44)  LDD1572  [16]
 LDCM0379  CL11 HEK-293T C320(1.56)  LDD1583  [16]
 LDCM0390  CL12 HEK-293T C67(1.15)  LDD1594  [16]
 LDCM0404  CL17 HEK-293T C320(1.59)  LDD1608  [16]
 LDCM0405  CL18 HEK-293T C320(1.56)  LDD1609  [16]
 LDCM0406  CL19 HEK-293T C320(1.98)  LDD1610  [16]
 LDCM0408  CL20 HEK-293T C320(1.18)  LDD1612  [16]
 LDCM0409  CL21 HEK-293T C473(0.76); C320(1.12)  LDD1613  [16]
 LDCM0410  CL22 HEK-293T C320(2.32)  LDD1614  [16]
 LDCM0411  CL23 HEK-293T C320(1.31)  LDD1615  [16]
 LDCM0412  CL24 HEK-293T C67(1.00)  LDD1616  [16]
 LDCM0417  CL29 HEK-293T C320(0.94)  LDD1621  [16]
 LDCM0419  CL30 HEK-293T C320(1.03)  LDD1623  [16]
 LDCM0420  CL31 HEK-293T C320(2.07)  LDD1624  [16]
 LDCM0421  CL32 HEK-293T C320(1.53)  LDD1625  [16]
 LDCM0422  CL33 HEK-293T C473(0.87); C320(1.00)  LDD1626  [16]
 LDCM0423  CL34 HEK-293T C320(1.75)  LDD1627  [16]
 LDCM0424  CL35 HEK-293T C320(1.46)  LDD1628  [16]
 LDCM0425  CL36 HEK-293T C67(1.12)  LDD1629  [16]
 LDCM0431  CL41 HEK-293T C320(1.91)  LDD1635  [16]
 LDCM0432  CL42 HEK-293T C320(1.58)  LDD1636  [16]
 LDCM0433  CL43 HEK-293T C320(1.54)  LDD1637  [16]
 LDCM0434  CL44 HEK-293T C320(1.46)  LDD1638  [16]
 LDCM0435  CL45 HEK-293T C473(0.66); C320(0.86)  LDD1639  [16]
 LDCM0436  CL46 HEK-293T C320(1.79)  LDD1640  [16]
 LDCM0437  CL47 HEK-293T C320(1.90)  LDD1641  [16]
 LDCM0438  CL48 HEK-293T C67(1.21)  LDD1642  [16]
 LDCM0440  CL5 HEK-293T C320(1.83)  LDD1644  [16]
 LDCM0444  CL53 HEK-293T C320(1.60)  LDD1647  [16]
 LDCM0445  CL54 HEK-293T C320(1.20)  LDD1648  [16]
 LDCM0446  CL55 HEK-293T C320(3.03)  LDD1649  [16]
 LDCM0447  CL56 HEK-293T C320(1.39)  LDD1650  [16]
 LDCM0448  CL57 HEK-293T C473(0.72); C320(0.87)  LDD1651  [16]
 LDCM0449  CL58 HEK-293T C320(1.81)  LDD1652  [16]
 LDCM0450  CL59 HEK-293T C320(1.45)  LDD1653  [16]
 LDCM0451  CL6 HEK-293T C320(1.53)  LDD1654  [16]
 LDCM0452  CL60 HEK-293T C67(1.17)  LDD1655  [16]
 LDCM0457  CL65 HEK-293T C320(1.18)  LDD1660  [16]
 LDCM0458  CL66 HEK-293T C320(1.63)  LDD1661  [16]
 LDCM0459  CL67 HEK-293T C320(1.41)  LDD1662  [16]
 LDCM0460  CL68 HEK-293T C320(1.22)  LDD1663  [16]
 LDCM0461  CL69 HEK-293T C473(0.93); C320(1.04)  LDD1664  [16]
 LDCM0462  CL7 HEK-293T C320(1.54)  LDD1665  [16]
 LDCM0463  CL70 HEK-293T C320(1.91)  LDD1666  [16]
 LDCM0464  CL71 HEK-293T C320(1.14)  LDD1667  [16]
 LDCM0465  CL72 HEK-293T C67(1.10)  LDD1668  [16]
 LDCM0470  CL77 HEK-293T C320(1.62)  LDD1673  [16]
 LDCM0471  CL78 HEK-293T C320(1.53)  LDD1674  [16]
 LDCM0472  CL79 HEK-293T C320(1.70)  LDD1675  [16]
 LDCM0473  CL8 HEK-293T C320(1.39)  LDD1676  [16]
 LDCM0474  CL80 HEK-293T C320(0.94)  LDD1677  [16]
 LDCM0475  CL81 HEK-293T C473(0.90); C320(1.34)  LDD1678  [16]
 LDCM0476  CL82 HEK-293T C320(1.80)  LDD1679  [16]
 LDCM0477  CL83 HEK-293T C320(1.97)  LDD1680  [16]
 LDCM0478  CL84 HEK-293T C67(0.87)  LDD1681  [16]
 LDCM0483  CL89 HEK-293T C320(1.83)  LDD1686  [16]
 LDCM0484  CL9 HEK-293T C473(0.84); C320(1.17)  LDD1687  [16]
 LDCM0485  CL90 HEK-293T C320(1.65)  LDD1688  [16]
 LDCM0486  CL91 HEK-293T C320(1.22)  LDD1689  [16]
 LDCM0487  CL92 HEK-293T C320(1.38)  LDD1690  [16]
 LDCM0488  CL93 HEK-293T C473(0.95); C320(1.03)  LDD1691  [16]
 LDCM0489  CL94 HEK-293T C320(1.13)  LDD1692  [16]
 LDCM0490  CL95 HEK-293T C320(1.13)  LDD1693  [16]
 LDCM0491  CL96 HEK-293T C67(1.15)  LDD1694  [16]
 LDCM0615  Fragment63-R Jurkat _(10.81)  LDD1487  [6]
 LDCM0617  Fragment63-S Jurkat _(20.00)  LDD1490  [6]
 LDCM0023  KB03 Jurkat C387(8.30)  LDD0209  [7]
 LDCM0628  OTUB2-COV-1 HEK-293T C319(1.12)  LDD2207  [17]
 LDCM0099  Phenelzine HEK-293T 3.13  LDD0282  [5]
 LDCM0136  SR-4559 HEK-293T 3.29  LDD0333  [15]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 3 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Nadh Small molecular drug DB00157
Tretinoin Small molecular drug DB00755
Vitamin A Small molecular drug DB00162

References

1 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
4 Discovery of Potent and Selective Inhibitors against Protein-Derived Electrophilic Cofactors. J Am Chem Soc. 2022 Mar 30;144(12):5377-5388. doi: 10.1021/jacs.1c12748. Epub 2022 Mar 2.
5 Activity-Based Hydrazine Probes for Protein Profiling of Electrophilic Functionality in Therapeutic Targets. ACS Cent Sci. 2021 Sep 22;7(9):1524-1534. doi: 10.1021/acscentsci.1c00616. Epub 2021 Aug 19.
6 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
7 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
8 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
9 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
10 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
11 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
12 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
13 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
14 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
15 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
16 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
17 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.