Details of the Target

General Information of Target

Target ID LDTP08859
Target Name Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4)
Gene Name GALNT4
Gene ID 100528030
Synonyms
Polypeptide N-acetylgalactosaminyltransferase 4; EC 2.4.1.41; Polypeptide GalNAc transferase 4; GalNAc-T4; pp-GaNTase 4; Protein-UDP acetylgalactosaminyltransferase 4; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAVRWTWAGKSCLLLAFLTVAYIFVELLVSTFHASAGAGRARELGSRRLSDLQKNTEDLS
RPLYKKPPADSRALGEWGKASKLQLNEDELKQQEELIERYAINIYLSDRISLHRHIEDKR
MYECKSQKFNYRTLPTTSVIIAFYNEAWSTLLRTIHSVLETSPAVLLKEIILVDDLSDRV
YLKTQLETYISNLDRVRLIRTNKREGLVRARLIGATFATGDVLTFLDCHCECNSGWLEPL
LERIGRDETAVVCPVIDTIDWNTFEFYMQIGEPMIGGFDWRLTFQWHSVPKQERDRRISR
IDPIRSPTMAGGLFAVSKKYFQYLGTYDTGMEVWGGENLELSFRVWQCGGKLEIHPCSHV
GHVFPKRAPYARPNFLQNTARAAEVWMDEYKEHFYNRNPPARKEAYGDISERKLLRERLR
CKSFDWYLKNVFPNLHVPEDRPGWHGAIRSRGISSECLDYNSPDNNPTGANLSLFGCHGQ
GGNQFFEYTSNKEIRFNSVTELCAEVPEQKNYVGMQNCPKDGFPVPANIIWHFKEDGTIF
HPHSGLCLSAYRTPEGRPDVQMRTCDALDKNQIWSFEK
Target Bioclass
Enzyme
Family
Glycosyltransferase 2 family, GalNAc-T subfamily
Subcellular location
Golgi apparatus membrane
Function
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. Glycosylates 'Thr-57' of SELPLG.
Uniprot ID
Q8N4A0
Ensemble ID
ENST00000529983.3
HGNC ID
HGNC:4126

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C518(3.85)  LDD3380  [1]
NAIA_5
 Probe Info  		C518(0.00); C565(0.00)  LDD2223  [2]
PAL-AfBPP Probe
Click To Hide/Show 44 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C004
 Probe Info  		7.01  LDD1714  [3]
C017
 Probe Info  		6.06  LDD1725  [3]
C040
 Probe Info  		11.08  LDD1740  [3]
C041
 Probe Info  		11.16  LDD1741  [3]
C055
 Probe Info  		17.88  LDD1752  [3]
C056
 Probe Info  		19.56  LDD1753  [3]
C059
 Probe Info  		8.28  LDD1756  [3]
C064
 Probe Info  		11.16  LDD1761  [3]
C082
 Probe Info  		5.24  LDD1774  [3]
C091
 Probe Info  		13.45  LDD1782  [3]
C092
 Probe Info  		16.45  LDD1783  [3]
C106
 Probe Info  		26.72  LDD1793  [3]
C107
 Probe Info  		12.21  LDD1794  [3]
C108
 Probe Info  		17.03  LDD1795  [3]
C130
 Probe Info  		12.04  LDD1812  [3]
C159
 Probe Info  		8.22  LDD1839  [3]
C160
 Probe Info  		9.45  LDD1840  [3]
C201
 Probe Info  		23.59  LDD1877  [3]
C206
 Probe Info  		20.39  LDD1881  [3]
C218
 Probe Info  		16.56  LDD1892  [3]
C219
 Probe Info  		10.56  LDD1893  [3]
C220
 Probe Info  		22.47  LDD1894  [3]
C225
 Probe Info  		8.69  LDD1898  [3]
C226
 Probe Info  		10.63  LDD1899  [3]
C228
 Probe Info  		20.82  LDD1901  [3]
C285
 Probe Info  		48.84  LDD1955  [3]
C286
 Probe Info  		13.64  LDD1956  [3]
C287
 Probe Info  		38.59  LDD1957  [3]
C288
 Probe Info  		19.84  LDD1958  [3]
C289
 Probe Info  		41.93  LDD1959  [3]
C290
 Probe Info  		15.67  LDD1960  [3]
C348
 Probe Info  		28.25  LDD2009  [3]
C349
 Probe Info  		31.34  LDD2010  [3]
C350
 Probe Info  		50.91  LDD2011  [3]
C353
 Probe Info  		12.55  LDD2014  [3]
C354
 Probe Info  		13.93  LDD2015  [3]
C407
 Probe Info  		12.21  LDD2064  [3]
C409
 Probe Info  		6.32  LDD2066  [3]
FFF probe11
 Probe Info  		20.00  LDD0471  [4]
FFF probe13
 Probe Info  		20.00  LDD0475  [4]
FFF probe14
 Probe Info  		20.00  LDD0477  [4]
FFF probe15
 Probe Info  		20.00  LDD0478  [4]
A-DA
 Probe Info  		2.13  LDD0142  [5]
OEA-DA
 Probe Info  		20.00  LDD0046  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0083  Avasimibe A-549 2.39  LDD0143  [5]
 LDCM0082  FK866 A-549 2.13  LDD0142  [5]
 LDCM0022  KB02 A2780 C518(2.65)  LDD2254  [1]
 LDCM0023  KB03 A2780 C518(3.99)  LDD2671  [1]
 LDCM0024  KB05 OV-90 C518(3.85)  LDD3380  [1]
 LDCM0084  Ro 48-8071 A-549 4.98  LDD0145  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
4 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
5 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.
6 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570