Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C226  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 95 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP16007  Lipid droplet-associated hydrolase (LDAH) 27.1
 LDTP00697  Cytochrome b5 type B (CYB5B) 24.9
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 22.9
 LDTP03257  Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) 20.4
 LDTP09395  Signal peptide peptidase-like 2A (SPPL2A) 18.1
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 18.0
 LDTP04004  Lysosomal Pro-X carboxypeptidase (PRCP) 17.9
 LDTP02359  Heme oxygenase 1 (HMOX1) 17.4
 LDTP16032  Retinoid-inducible serine carboxypeptidase (SCPEP1) 16.8
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 16.8
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 16.4
 LDTP03540  Heme oxygenase 2 (HMOX2) 16.2
 LDTP01982  NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) 14.9
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 14.8
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 14.5
 LDTP10329  Ceramide synthase 2 (CERS2) 14.2
 LDTP02837  Beta-galactosidase (GLB1) 13.4
 LDTP15484  Saccharopine dehydrogenase-like oxidoreductase (SCCPDH) 13.4
 LDTP13358  Leucyl-cystinyl aminopeptidase (LNPEP) 12.5
 LDTP06477  Methylsterol monooxygenase 1 (MSMO1) 12.4
 LDTP06333  Serum paraoxonase/arylesterase 2 (PON2) 11.9
 LDTP12363  Lysophosphatidic acid phosphatase type 6 (ACP6) 11.2
 LDTP06200  Delta(14)-sterol reductase LBR (LBR) 11.0
 LDTP11259  Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase (ALG12) 10.9
 LDTP15988  Probable serine carboxypeptidase CPVL (CPVL) 10.9
 LDTP09256  Putative phospholipase B-like 2 (PLBD2) 10.9
 LDTP04581  Holocytochrome c-type synthase (HCCS) 10.8
 LDTP08859  Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4) 10.6
 LDTP04284  Proteasome subunit beta type-3 (PSMB3) 10.6
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 10.5
 LDTP02505  Lysosomal protective protein (CTSA) 10.4
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 10.3
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 10.2
 LDTP10188  FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) 9.9
 LDTP01389  Delta(14)-sterol reductase TM7SF2 (TM7SF2) 9.8
 LDTP08265  N-alpha-acetyltransferase 40 (NAA40) 9.4
 LDTP00486  Cytochrome b-c1 complex subunit 8 (UQCRQ) 9.3
 LDTP13189  Cytochrome b-c1 complex subunit 9 (UQCR10) 9.2
 LDTP02005  Lysosomal acid glucosylceramidase (GBA1) 9.1
 LDTP10550  Dehydrogenase/reductase SDR family member 1 (DHRS1) 9.0
 LDTP00428  Tripeptidyl-peptidase 1 (TPP1) 8.8
 LDTP17410  Cytochrome P450 20A1 (CYP20A1) 8.7
 LDTP03813  Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial (CPOX) 8.5
 LDTP09396  Minor histocompatibility antigen H13 (HM13) 8.3
 LDTP04051  Nicotinamide phosphoribosyltransferase (NAMPT) 8.2
 LDTP12763  Golgi-resident adenosine 3',5'-bisphosphate 3'-phosphatase (BPNT2) 8.1
 LDTP04008  Endothelin-converting enzyme 1 (ECE1) 8.0
 LDTP11867  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) 7.9
 LDTP08476  5'-3' exonuclease PLD3 (PLD3) 7.8
 LDTP03394  Ceramide synthase 1 (CERS1) 7.8
 LDTP02254  ATP-dependent translocase ABCB1 (ABCB1) 7.7
 LDTP03787  Choline kinase alpha (CHKA) 7.7
 LDTP00783  Beta-1,4-glucuronyltransferase 1 (B4GAT1) 7.6
 LDTP01773  Cytochrome c oxidase subunit 2 (MT-CO2) 7.1
 LDTP08728  Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) 7.1
 LDTP12253  Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM) 6.9
 LDTP06720  Peroxisomal leader peptide-processing protease (TYSND1) 6.9
 LDTP03139  Succinate dehydrogenase iron-sulfur subunit, mitochondrial (SDHB) 6.8
 LDTP01769  Dihydrofolate reductase (DHFR) 6.8
 LDTP12105  L-2-hydroxyglutarate dehydrogenase, mitochondrial (L2HGDH) 6.7
 LDTP01613  Sphingosine-1-phosphate lyase 1 (SGPL1) 6.7
 LDTP15480  Prenylcysteine oxidase-like (PCYOX1L) 6.6
 LDTP01241  Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) 6.5
 LDTP09088  Phospholipase A2 group XV (PLA2G15) 6.5
 LDTP03912  Trifunctional enzyme subunit alpha, mitochondrial (HADHA) 6.5
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 6.5
 LDTP07620  Peroxisomal N(1)-acetyl-spermine/spermidine oxidase (PAOX) 6.4
 LDTP13486  Long-chain-fatty-acid--CoA ligase 6 (ACSL6) 6.3
 LDTP13620  Lysosomal thioesterase PPT2 (PPT2) 6.3
 LDTP15328  Dolichyldiphosphatase 1 (DOLPP1) 6.3
 LDTP10612  Lysophospholipid acyltransferase 7 (MBOAT7) 6.2
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 6.2
 LDTP04712  Puromycin-sensitive aminopeptidase (NPEPPS) 6.2
 LDTP06384  Ribosomal protein S6 kinase alpha-1 (RPS6KA1) 6.2
 LDTP06787  Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) 6.1
 LDTP08385  NADH dehydrogenase 1 alpha subcomplex subunit 11 (NDUFA11) 6.1
 LDTP08617  Patatin-like phospholipase domain-containing protein 6 (PNPLA6) 6.1
 LDTP02260  Beta-glucuronidase (GUSB) 6.0
 LDTP05228  Calcium-transporting ATPase type 2C member 1 (ATP2C1) 6.0
 LDTP04247  Protein farnesyltransferase subunit beta (FNTB) 6.0
 LDTP06067  Tubulin--tyrosine ligase-like protein 12 (TTLL12) 6.0
 LDTP00032  2-hydroxyacyl-CoA lyase 2 (ILVBL) 5.9
 LDTP12679  Trimethyllysine dioxygenase, mitochondrial (TMLHE) 5.9
 LDTP11127  ADP-dependent glucokinase (ADPGK) 5.9
 LDTP06648  Lanosterol 14-alpha demethylase (CYP51A1) 5.8
 LDTP01462  Endonuclease domain-containing 1 protein (ENDOD1) 5.7
 LDTP15097  All-trans-retinol 13,14-reductase (RETSAT) 5.7
 LDTP10182  TLC domain-containing protein 1 (TLCD1) 5.7
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 5.6
 LDTP13751  Exonuclease 1 (EXO1) 5.6
 LDTP12639  1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (AGPAT5) 5.6
 LDTP05476  3-ketodihydrosphingosine reductase (KDSR) 5.6
 LDTP12873  CDGSH iron-sulfur domain-containing protein 1 (CISD1) 5.5
 LDTP07990  Heparan sulfate 2-O-sulfotransferase 1 (HS2ST1) 5.5
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 5.5
Transporter and channel
Click to Show/Hide to Show 76 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 99.7
 LDTP01804  ATP synthase subunit a (MT-ATP6) 28.8
 LDTP15948  Transmembrane protein 126A (TMEM126A) 26.0
 LDTP06945  Sigma intracellular receptor 2 (TMEM97) 23.6
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 22.5
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 22.3
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 21.6
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 21.0
 LDTP11615  Protein tweety homolog 3 (TTYH3) 18.8
 LDTP16813  Tetraspanin-3 (TSPAN3) 18.0
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 17.4
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 17.0
 LDTP11927  Essential MCU regulator, mitochondrial (SMDT1) 15.6
 LDTP15481  Transmembrane protein 87A (TMEM87A) 15.5
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 15.3
 LDTP20002  Transmembrane protein 160 (TMEM160) 15.0
 LDTP15967  Tetraspanin-10 (TSPAN10) 14.0
 LDTP07236  Protein GPR107 (GPR107) 13.7
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 13.6
 LDTP08881  Transmembrane protein 199 (TMEM199) 13.0
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 12.8
 LDTP05900  Metaxin-1 (MTX1) 12.3
 LDTP11162  Solute carrier family 25 member 33 (SLC25A33) 12.1
 LDTP15648  BRI3-binding protein (BRI3BP) 12.0
 LDTP16069  Peroxisomal membrane protein 2 (PXMP2) 12.0
 LDTP02215  Prosaposin (PSAP) 11.7
 LDTP16190  Protein FAM8A1 (FAM8A1) 11.6
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 11.3
 LDTP18153  Major facilitator superfamily domain-containing protein 3 (MFSD3) 11.2
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 11.2
 LDTP00865  Mitochondrial carnitine/acylcarnitine carrier protein (SLC25A20) 10.6
 LDTP00262  Acetyl-coenzyme A transporter 1 (SLC33A1) 10.5
 LDTP00235  Membrane-associated progesterone receptor component 1 (PGRMC1) 10.0
 LDTP00541  NPC intracellular cholesterol transporter 1 (NPC1) 9.5
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 9.4
 LDTP08279  Mitochondrial coenzyme A transporter SLC25A42 (SLC25A42) 9.2
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 9.2
 LDTP14200  Mitochondrial ornithine transporter 1 (SLC25A15) 9.2
 LDTP12451  Integral membrane protein 2C (ITM2C) 9.1
 LDTP12383  Protein GPR108 (GPR108) 9.0
 LDTP09294  Proton-coupled zinc antiporter SLC30A5 (SLC30A5) 8.5
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 8.3
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 8.2
 LDTP11213  Nucleoporin NDC1 (NDC1) 8.1
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 7.9
 LDTP00592  Surfeit locus protein 4 (SURF4) 7.9
 LDTP11095  Mitochondrial 2-oxodicarboxylate carrier (SLC25A21) 7.8
 LDTP09180  Zinc transporter 7 (SLC30A7) 7.7
 LDTP13734  Cystine/glutamate transporter (SLC7A11) 7.6
 LDTP14235  Mitochondrial carrier homolog 2 (MTCH2) 7.6
 LDTP02655  Lysosome-associated membrane glycoprotein 2 (LAMP2) 7.5
 LDTP01745  Putative lipid scramblase CLPTM1 (CLPTM1) 7.5
 LDTP09028  Mitoguardin 1 (MIGA1) 7.4
 LDTP10065  Transmembrane protein 230 (TMEM230) 7.2
 LDTP11245  Derlin-1 (DERL1) 6.9
 LDTP09328  Adenosine 3'-phospho 5'-phosphosulfate transporter 1 (SLC35B2) 6.7
 LDTP06308  Mitochondrial inner membrane protein OXA1L (OXA1L) 6.7
 LDTP02068  Sodium/potassium-transporting ATPase subunit beta-1 (ATP1B1) 6.5
 LDTP11844  Protein spinster homolog 1 (SPNS1) 6.4
 LDTP03559  High affinity cationic amino acid transporter 1 (SLC7A1) 6.3
 LDTP11406  Transmembrane protein 59 (TMEM59) 6.2
 LDTP07439  Mitochondrial adenyl nucleotide antiporter SLC25A25 (SLC25A25) 6.1
 LDTP00477  Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) 6.1
 LDTP06221  StAR-related lipid transfer protein 3 (STARD3) 6.1
 LDTP11116  45 kDa calcium-binding protein (SDF4) 6.1
 LDTP03380  Stomatin (STOM) 6.1
 LDTP17641  Solute carrier family 35 member F2 (SLC35F2) 5.9
 LDTP02562  Lysosome-associated membrane glycoprotein 1 (LAMP1) 5.8
 LDTP10059  PAT complex subunit CCDC47 (CCDC47) 5.8
 LDTP08469  Complex I assembly factor TMEM126B, mitochondrial (TMEM126B) 5.8
 LDTP04789  Synaptojanin-2-binding protein (SYNJ2BP) 5.8
 LDTP18053  Probable mitochondrial glutathione transporter SLC25A40 (SLC25A40) 5.7
 LDTP07156  Protein wntless homolog (WLS) 5.6
 LDTP10499  Lipid scramblase CLPTM1L (CLPTM1L) 5.6
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 5.5
 LDTP12210  Mitochondrial thiamine pyrophosphate carrier (SLC25A19) 5.5
GPCR
Click to Show/Hide to Show 1 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP15303  Membrane progestin receptor alpha (PAQR7) 12.4
Other
Click to Show/Hide to Show 51 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP11076  Apolipoprotein L2 (APOL2) 30.3
 LDTP03678  ER lumen protein-retaining receptor 2 (KDELR2) 28.4
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 22.0
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 21.6
 LDTP13606  Mammalian ependymin-related protein 1 (EPDR1) 20.7
 LDTP00850  ER lumen protein-retaining receptor 3 (KDELR3) 18.6
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 18.4
 LDTP05160  Nucleobindin-2 (NUCB2) 17.6
 LDTP05375  Nucleobindin-1 (NUCB1) 16.6
 LDTP01522  Reticulon-3 (RTN3) 15.3
 LDTP08796  Protein SYS1 homolog (SYS1) 13.8
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 13.2
 LDTP16099  p53 and DNA damage-regulated protein 1 (PDRG1) 12.8
 LDTP14458  Tetraspanin-6 (TSPAN6) 11.0
 LDTP10056  Protein FAM162A (FAM162A) 10.4
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 10.0
 LDTP12025  UPF0488 protein C8orf33 (C8orf33) 9.9
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 9.8
 LDTP04652  Eukaryotic translation initiation factor 5 (EIF5) 9.6
 LDTP05868  Protein unc-119 homolog A (UNC119) 9.6
 LDTP12412  Reticulon-4 (RTN4) 9.6
 LDTP08130  Rab9 effector protein with kelch motifs (RABEPK) 8.8
 LDTP08224  Protein LYRIC (MTDH) 8.7
 LDTP13802  DNA replication complex GINS protein PSF2 (GINS2) 8.4
 LDTP14939  Membralin (TMEM259) 8.2
 LDTP16006  Optic atrophy 3 protein (OPA3) 8.2
 LDTP16080  Protein FAM114A2 (FAM114A2) 8.1
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 8.0
 LDTP18883  Protein CEBPZOS (CEBPZOS) 7.9
 LDTP06404  Surfeit locus protein 1 (SURF1) 7.8
 LDTP13755  Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2) 7.5
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 7.3
 LDTP19021  EF-hand calcium-binding domain-containing protein 14 (EFCAB14) 7.0
 LDTP03926  Centrin-2 (CETN2) 6.9
 LDTP15634  Ubiquinol-cytochrome c reductase complex assembly factor 5 (UQCC5) 6.8
 LDTP13086  Syntaxin-18 (STX18) 6.7
 LDTP02443  Calmodulin-3 (CALM3) 6.6
 LDTP10116  Lipid droplet assembly factor 1 (LDAF1) 6.3
 LDTP18373  Thioredoxin-related transmembrane protein 4 (TMX4) 6.2
 LDTP15434  ADP-ribosylation factor-like protein 6-interacting protein 6 (ARL6IP6) 6.1
 LDTP19963  TraB domain-containing protein (TRABD) 6.1
 LDTP18272  Transmembrane protein 209 (TMEM209) 6.1
 LDTP06097  Reticulocalbin-2 (RCN2) 6.1
 LDTP09881  TATA-binding protein-associated factor 2N (TAF15) 5.8
 LDTP13630  Neudesin (NENF) 5.7
 LDTP10381  Peroxisomal membrane protein 11C (PEX11G) 5.6
 LDTP08904  Protein jagunal homolog 1 (JAGN1) 5.6
 LDTP16323  Signal recognition particle receptor subunit beta (SRPRB) 5.6
 LDTP05922  Dynactin subunit 2 (DCTN2) 5.5
 LDTP02927  Ganglioside GM2 activator (GM2A) 5.5
 LDTP19780  Keratinocyte-associated transmembrane protein 2 (KCT2) 5.5

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587