Details of the Target

General Information of Target

Target ID LDTP13486
Target Name Long-chain-fatty-acid--CoA ligase 6 (ACSL6)
Gene Name ACSL6
Gene ID 23305
Synonyms
ACS2; FACL6; KIAA0837; LACS5; Long-chain-fatty-acid--CoA ligase 6; EC 6.2.1.3; Arachidonate--CoA ligase; EC 6.2.1.15; Long-chain acyl-CoA synthetase 6; LACS 6
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEDIQTNAELKSTQEQSVPAESAAVLNDYSLTKSHEMENVDSGEGPANEDEDIGDDSMKV
KDEYSERDENVLKSEPMGNAEEPEIPYSYSREYNEYENIKLERHVVSFDSSRPTSGKMNC
DVCGLSCISFNVLMVHKRSHTGERPFQCNQCGASFTQKGNLLRHIKLHTGEKPFKCHLCN
YACQRRDALTGHLRTHSVEKPYKCEFCGRSYKQRSSLEEHKERCRTFLQSTDPGDTASAE
ARHIKAEMGSERALVLDRLASNVAKRKSSMPQKFIGEKRHCFDVNYNSSYMYEKESELIQ
TRMMDQAINNAISYLGAEALRPLVQTPPAPTSEMVPVISSMYPIALTRAEMSNGAPQELE
KKSIHLPEKSVPSERGLSPNNSGHDSTDTDSNHEERQNHIYQQNHMVLSRARNGMPLLKE
VPRSYELLKPPPICPRDSVKVINKEGEVMDVYRCDHCRVLFLDYVMFTIHMGCHGFRDPF
ECNMCGYRSHDRYEFSSHIARGEHRALLK
Target Bioclass
Enzyme
Family
ATP-dependent AMP-binding enzyme family
Subcellular location
Mitochondrion outer membrane
Function
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.
Uniprot ID
Q9UKU0
Ensemble ID
ENST00000357096.5
HGNC ID
HGNC:16496
ChEMBL ID
CHEMBL4680042

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
C4II SNV: p.T222N .
DU145 SNV: p.L694F .
HEC1 SNV: p.Q446R; p.I683T .
LNCaP clone FGC SNV: p.A419T .
MCC13 SNV: p.G590R
Substitution: p.S272F		 .
MFE319 Deletion: p.K582SfsTer37 .
NCIH146 SNV: p.G364V .
NCIH2286 SNV: p.W185C .
NCIH661 SNV: p.G367S .
SW1271 SNV: p.K381N .
SW480 SNV: p.I186L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C167(1.38); C650(1.40); C452(2.12)  LDD3430  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
PAL-AfBPP Probe
Click To Hide/Show 50 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C017
 Probe Info  		7.73  LDD1725  [3]
C027
 Probe Info  		7.26  LDD1733  [3]
C040
 Probe Info  		14.12  LDD1740  [3]
C041
 Probe Info  		6.59  LDD1741  [3]
C055
 Probe Info  		22.78  LDD1752  [3]
C056
 Probe Info  		36.25  LDD1753  [3]
C063
 Probe Info  		17.88  LDD1760  [3]
C064
 Probe Info  		6.36  LDD1761  [3]
C070
 Probe Info  		15.67  LDD1766  [3]
C072
 Probe Info  		7.78  LDD1768  [3]
C106
 Probe Info  		26.35  LDD1793  [3]
C107
 Probe Info  		7.67  LDD1794  [3]
C108
 Probe Info  		11.39  LDD1795  [3]
C112
 Probe Info  		28.64  LDD1799  [3]
C134
 Probe Info  		22.16  LDD1816  [3]
C135
 Probe Info  		10.41  LDD1817  [3]
C201
 Probe Info  		42.22  LDD1877  [3]
C206
 Probe Info  		27.28  LDD1881  [3]
C210
 Probe Info  		52.35  LDD1884  [3]
C211
 Probe Info  		9.58  LDD1885  [3]
C213
 Probe Info  		16.91  LDD1887  [3]
C225
 Probe Info  		6.45  LDD1898  [3]
C226
 Probe Info  		6.32  LDD1899  [3]
C228
 Probe Info  		27.10  LDD1901  [3]
C243
 Probe Info  		11.63  LDD1916  [3]
C246
 Probe Info  		13.27  LDD1919  [3]
C252
 Probe Info  		9.65  LDD1925  [3]
C264
 Probe Info  		25.81  LDD1935  [3]
C265
 Probe Info  		17.03  LDD1936  [3]
C270
 Probe Info  		11.47  LDD1940  [3]
C285
 Probe Info  		62.25  LDD1955  [3]
C286
 Probe Info  		7.89  LDD1956  [3]
C287
 Probe Info  		33.13  LDD1957  [3]
C288
 Probe Info  		16.91  LDD1958  [3]
C289
 Probe Info  		80.45  LDD1959  [3]
C290
 Probe Info  		9.38  LDD1960  [3]
C293
 Probe Info  		17.63  LDD1963  [3]
C296
 Probe Info  		14.03  LDD1966  [3]
C314
 Probe Info  		14.83  LDD1981  [3]
C343
 Probe Info  		12.55  LDD2005  [3]
C349
 Probe Info  		17.15  LDD2010  [3]
C350
 Probe Info  		26.91  LDD2011  [3]
C362
 Probe Info  		31.56  LDD2023  [3]
C366
 Probe Info  		7.31  LDD2027  [3]
C382
 Probe Info  		9.65  LDD2041  [3]
C383
 Probe Info  		15.56  LDD2042  [3]
C386
 Probe Info  		7.36  LDD2045  [3]
C388
 Probe Info  		56.49  LDD2047  [3]
C431
 Probe Info  		14.83  LDD2086  [3]
OEA-DA
 Probe Info  		20.00  LDD0046  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0371  CL102 HEK-293T C502(0.87)  LDD1575  [5]
 LDCM0375  CL106 HEK-293T C502(1.00)  LDD1579  [5]
 LDCM0380  CL110 HEK-293T C502(0.84)  LDD1584  [5]
 LDCM0384  CL114 HEK-293T C502(0.83)  LDD1588  [5]
 LDCM0388  CL118 HEK-293T C502(0.94)  LDD1592  [5]
 LDCM0393  CL122 HEK-293T C502(0.92)  LDD1597  [5]
 LDCM0397  CL126 HEK-293T C502(0.97)  LDD1601  [5]
 LDCM0401  CL14 HEK-293T C502(1.00)  LDD1605  [5]
 LDCM0407  CL2 HEK-293T C502(1.05)  LDD1611  [5]
 LDCM0414  CL26 HEK-293T C502(0.94)  LDD1618  [5]
 LDCM0441  CL50 HEK-293T C502(0.89)  LDD1645  [5]
 LDCM0454  CL62 HEK-293T C502(0.92)  LDD1657  [5]
 LDCM0467  CL74 HEK-293T C502(1.00)  LDD1670  [5]
 LDCM0480  CL86 HEK-293T C502(0.84)  LDD1683  [5]
 LDCM0493  CL98 HEK-293T C502(0.87)  LDD1696  [5]
 LDCM0427  Fragment51 HEK-293T C502(0.92)  LDD1631  [5]
 LDCM0022  KB02 A101D C167(1.14); C384(1.23); C80(1.20); C534(1.30)  LDD2250  [1]
 LDCM0023  KB03 A101D C167(1.38); C384(3.20); C80(1.67); C534(2.05)  LDD2667  [1]
 LDCM0024  KB05 SK-MEL-5 C167(1.38); C650(1.40); C452(2.12)  LDD3430  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein-L-isoaspartate O-methyltransferase domain-containing protein 2 (PCMTD2) L-isoaspartyl/D-aspartyl protein methyltransferase family Q9NV79
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein 655 (ZNF655) Krueppel C2H2-type zinc-finger protein family Q8N720

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
4 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402