Details of the Probe-central Interaction Altas

General Information of Experiment

Probe Name C265  Probe Info 
Probe concentration Probe:50uM; negative probe:50uM
Criteria Quantification: AfBPP Probe vs Negative Probe
Quantitative Method
TMT
In Vivo Experiment Model Model Type Living cell
Derived Tissue Peripheral blood
Disease Model Normal
Model Name Human normal cells (HEK-293T)
Note Negative probe:C#CCCC1(CCC(NC)=O)N=N1

Interaction Altas of This Experiment [1]

Enzyme
Click to Show/Hide to Show 118 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP11321  Acetyl-CoA acetyltransferase, cytosolic (ACAT2) 99.0
 LDTP04051  Nicotinamide phosphoribosyltransferase (NAMPT) 68.1
 LDTP06645  Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (HADH) 42.8
 LDTP16007  Lipid droplet-associated hydrolase (LDAH) 40.5
 LDTP14218  Peptidyl-prolyl cis-trans isomerase FKBP7 (FKBP7) 40.2
 LDTP06227  Prostaglandin reductase 1 (PTGR1) 40.2
 LDTP05128  Glutathione S-transferase omega-1 (GSTO1) 36.8
 LDTP15422  Prostaglandin reductase 3 (PTGR3) 35.8
 LDTP01039  Protein-S-isoprenylcysteine O-methyltransferase (ICMT) 35.5
 LDTP00697  Cytochrome b5 type B (CYB5B) 35.0
 LDTP13282  Prenylcysteine oxidase 1 (PCYOX1) 34.3
 LDTP02005  Lysosomal acid glucosylceramidase (GBA1) 33.4
 LDTP12013  dCTP pyrophosphatase 1 (DCTPP1) 32.9
 LDTP02359  Heme oxygenase 1 (HMOX1) 32.4
 LDTP12628  Palmitoyl-protein thioesterase ABHD10, mitochondrial (ABHD10) 32.2
 LDTP03540  Heme oxygenase 2 (HMOX2) 31.8
 LDTP12363  Lysophosphatidic acid phosphatase type 6 (ACP6) 31.6
 LDTP00486  Cytochrome b-c1 complex subunit 8 (UQCRQ) 30.7
 LDTP10188  FAD-dependent oxidoreductase domain-containing protein 1 (FOXRED1) 30.3
 LDTP04388  Palmitoyl-protein thioesterase 1 (PPT1) 29.7
 LDTP16032  Retinoid-inducible serine carboxypeptidase (SCPEP1) 28.6
 LDTP04285  Proteasome subunit beta type-2 (PSMB2) 28.4
 LDTP12610  Obg-like ATPase 1 (OLA1) 27.7
 LDTP10032  7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) 27.5
 LDTP12873  CDGSH iron-sulfur domain-containing protein 1 (CISD1) 26.5
 LDTP12197  Ethanolamine kinase 1 (ETNK1) 26.5
 LDTP05193  ADP-ribosylation factor 5 (ARF5) 26.2
 LDTP12959  NADH dehydrogenase 1 alpha subcomplex subunit 13 (NDUFA13) 26.2
 LDTP11273  N-terminal Xaa-Pro-Lys N-methyltransferase 1 (NTMT1) 26.0
 LDTP14276  NADH dehydrogenase 1 beta subcomplex subunit 9 (NDUFB9) 25.3
 LDTP01982  NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) 25.3
 LDTP04437  Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) 24.9
 LDTP13189  Cytochrome b-c1 complex subunit 9 (UQCR10) 24.9
 LDTP13620  Lysosomal thioesterase PPT2 (PPT2) 24.6
 LDTP13358  Leucyl-cystinyl aminopeptidase (LNPEP) 24.4
 LDTP12679  Trimethyllysine dioxygenase, mitochondrial (TMLHE) 23.6
 LDTP01328  CAAX prenyl protease 1 homolog (ZMPSTE24) 23.3
 LDTP12163  Calcyclin-binding protein (CACYBP) 23.3
 LDTP10550  Dehydrogenase/reductase SDR family member 1 (DHRS1) 23.1
 LDTP06618  ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) 22.8
 LDTP06787  Inactive hydroxysteroid dehydrogenase-like protein 1 (HSDL1) 22.8
 LDTP10101  Peptidyl-prolyl cis-trans isomerase FKBP10 (FKBP10) 22.6
 LDTP05905  Acid ceramidase (ASAH1) 22.3
 LDTP09096  Carbohydrate sulfotransferase 14 (CHST14) 22.3
 LDTP00988  Protein O-GlcNAcase (OGA) 22.2
 LDTP04581  Holocytochrome c-type synthase (HCCS) 21.9
 LDTP01562  Peptidyl-prolyl cis-trans isomerase FKBP9 (FKBP9) 21.9
 LDTP03522  Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A) 21.9
 LDTP06375  Delta(24)-sterol reductase (DHCR24) 21.6
 LDTP00832  NADH dehydrogenase 1 beta subcomplex subunit 3 (NDUFB3) 21.6
 LDTP06617  Ceramide glucosyltransferase (UGCG) 21.0
 LDTP01241  Bis(monoacylglycero)phosphate synthase CLN5 (CLN5) 20.8
 LDTP09396  Minor histocompatibility antigen H13 (HM13) 20.7
 LDTP09256  Putative phospholipase B-like 2 (PLBD2) 20.7
 LDTP03523  Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform (PPP2R1B) 20.4
 LDTP12105  L-2-hydroxyglutarate dehydrogenase, mitochondrial (L2HGDH) 20.3
 LDTP12415  Adenosine 5'-monophosphoramidase HINT3 (HINT3) 20.1
 LDTP02942  ADP-ribosylation factor 4 (ARF4) 20.1
 LDTP14814  CTD small phosphatase-like protein 2 (CTDSPL2) 20.1
 LDTP15879  Haloacid dehalogenase-like hydrolase domain-containing protein 3 (HDHD3) 20.1
 LDTP12279  Endoplasmic reticulum transmembrane helix translocase (ATP13A1) 20.0
 LDTP00950  Dynamin-like 120 kDa protein, mitochondrial (OPA1) 19.3
 LDTP05066  Signal peptidase complex catalytic subunit SEC11A (SEC11A) 19.2
 LDTP09095  Diacylglycerol lipase-beta (DAGLB) 19.0
 LDTP17305  Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) 19.0
 LDTP15328  Dolichyldiphosphatase 1 (DOLPP1) 18.9
 LDTP06477  Methylsterol monooxygenase 1 (MSMO1) 18.6
 LDTP12868  Endoplasmic reticulum aminopeptidase 1 (ERAP1) 18.3
 LDTP05029  Peptidyl-prolyl cis-trans isomerase FKBP1A (FKBP1A) 18.1
 LDTP10887  Synaptic vesicle membrane protein VAT-1 homolog (VAT1) 17.5
 LDTP03145  Catechol O-methyltransferase (COMT) 17.4
 LDTP09666  Reticulon-4-interacting protein 1, mitochondrial (RTN4IP1) 17.1
 LDTP13486  Long-chain-fatty-acid--CoA ligase 6 (ACSL6) 17.0
 LDTP02505  Lysosomal protective protein (CTSA) 16.8
 LDTP05408  Antigen peptide transporter 1 (TAP1) 16.7
 LDTP11127  ADP-dependent glucokinase (ADPGK) 16.6
 LDTP00428  Tripeptidyl-peptidase 1 (TPP1) 16.6
 LDTP08728  Vitamin K epoxide reductase complex subunit 1-like protein 1 (VKORC1L1) 16.4
 LDTP08476  5'-3' exonuclease PLD3 (PLD3) 16.2
 LDTP10182  TLC domain-containing protein 1 (TLCD1) 15.9
 LDTP01773  Cytochrome c oxidase subunit 2 (MT-CO2) 15.8
 LDTP02260  Beta-glucuronidase (GUSB) 15.7
 LDTP07525  Protein arginine N-methyltransferase 9 (PRMT9) 15.7
 LDTP06501  Ras-related protein Rab-11B (RAB11B) 15.6
 LDTP10981  Mitochondrial intermediate peptidase (MIPEP) 15.5
 LDTP07868  Protein O-mannosyl-transferase TMTC3 (TMTC3) 15.5
 LDTP01613  Sphingosine-1-phosphate lyase 1 (SGPL1) 15.5
 LDTP08790  Mitochondrial cardiolipin hydrolase (PLD6) 15.3
 LDTP04008  Endothelin-converting enzyme 1 (ECE1) 15.2
 LDTP00654  Synaptobrevin homolog YKT6 (YKT6) 15.2
 LDTP07503  Endoplasmic reticulum aminopeptidase 2 (ERAP2) 15.1
 LDTP04243  Fatty acid synthase (FASN) 15.1
 LDTP06200  Delta(14)-sterol reductase LBR (LBR) 15.0
 LDTP05624  Polypeptide N-acetylgalactosaminyltransferase 1 (GALNT1) 14.6
 LDTP04634  Alpha-N-acetylglucosaminidase (NAGLU) 14.4
 LDTP06901  Very-long-chain 3-oxoacyl-CoA reductase (HSD17B12) 14.4
 LDTP02385  Leukotriene A-4 hydrolase (LTA4H) 14.3
 LDTP05632  Mitochondrial-processing peptidase subunit alpha (PMPCA) 14.3
 LDTP09874  Endoplasmic reticulum protein SC65 (P3H4) 14.1
 LDTP02175  Epoxide hydrolase 1 (EPHX1) 14.1
 LDTP12215  rRNA methyltransferase 3, mitochondrial (MRM3) 14.0
 LDTP06849  Prolyl endopeptidase-like (PREPL) 13.9
 LDTP02223  Cathepsin B (CTSB) 13.8
 LDTP05446  Ubiquitin-protein ligase E3A (UBE3A) 13.8
 LDTP08385  NADH dehydrogenase 1 alpha subcomplex subunit 11 (NDUFA11) 13.7
 LDTP03257  Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2) 13.6
 LDTP06307  Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) 13.6
 LDTP04032  Glycerol-3-phosphate dehydrogenase, mitochondrial (GPD2) 13.5
 LDTP01707  Thioredoxin domain-containing protein 12 (TXNDC12) 13.5
 LDTP11733  Ras-related protein Rab-1B (RAB1B) 13.4
 LDTP14030  V-type proton ATPase 116 kDa subunit a 2 (ATP6V0A2) 13.4
 LDTP13220  Phosphatidylserine decarboxylase proenzyme, mitochondrial (PISD) 13.3
 LDTP01300  Cartilage-associated protein (CRTAP) 13.2
 LDTP04531  Hexokinase-2 (HK2) 13.1
 LDTP05476  3-ketodihydrosphingosine reductase (KDSR) 13.0
 LDTP01335  Protein SCO1 homolog, mitochondrial (SCO1) 13.0
 LDTP05140  Arginase-2, mitochondrial (ARG2) 12.9
 LDTP07990  Heparan sulfate 2-O-sulfotransferase 1 (HS2ST1) 12.8
Transporter and channel
Click to Show/Hide to Show 68 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP08881  Transmembrane protein 199 (TMEM199) 88.0
 LDTP20002  Transmembrane protein 160 (TMEM160) 67.6
 LDTP01059  Mitochondrial import inner membrane translocase subunit Tim17-B (TIMM17B) 61.8
 LDTP10913  Mitochondrial import inner membrane translocase subunit Tim17-A (TIMM17A) 54.6
 LDTP04033  Translocon-associated protein subunit alpha (SSR1) 52.7
 LDTP02724  Cytochrome c oxidase subunit 7A2, mitochondrial (COX7A2) 50.6
 LDTP14200  Mitochondrial ornithine transporter 1 (SLC25A15) 49.5
 LDTP15948  Transmembrane protein 126A (TMEM126A) 48.5
 LDTP14952  Mpv17-like protein 2 (MPV17L2) 47.2
 LDTP07885  Mitochondrial S-adenosylmethionine carrier protein (SLC25A26) 44.6
 LDTP03132  Voltage-dependent anion-selective channel protein 1 (VDAC1) 43.1
 LDTP06633  Reticulon-1 (RTN1) 38.3
 LDTP16813  Tetraspanin-3 (TSPAN3) 37.5
 LDTP11813  Solute carrier family 25 member 32 (SLC25A32) 36.8
 LDTP04956  Mitochondrial import inner membrane translocase subunit Tim10 (TIMM10) 33.6
 LDTP11162  Solute carrier family 25 member 33 (SLC25A33) 31.3
 LDTP04071  Voltage-dependent anion-selective channel protein 2 (VDAC2) 30.7
 LDTP01804  ATP synthase subunit a (MT-ATP6) 27.3
 LDTP18153  Major facilitator superfamily domain-containing protein 3 (MFSD3) 25.3
 LDTP14511  ATP synthase subunit g, mitochondrial (ATP5MG) 24.6
 LDTP03277  ER lumen protein-retaining receptor 1 (KDELR1) 23.9
 LDTP13174  Mitochondrial dicarboxylate carrier (SLC25A10) 22.8
 LDTP16069  Peroxisomal membrane protein 2 (PXMP2) 22.8
 LDTP13983  Mitochondrial import inner membrane translocase subunit TIM16 (PAM16) 22.6
 LDTP15481  Transmembrane protein 87A (TMEM87A) 22.6
 LDTP00562  Membrane-associated progesterone receptor component 2 (PGRMC2) 22.2
 LDTP14099  Mitochondrial import inner membrane translocase subunit Tim22 (TIMM22) 21.6
 LDTP02215  Prosaposin (PSAP) 21.4
 LDTP14181  Peroxisomal membrane protein PEX16 (PEX16) 21.0
 LDTP07236  Protein GPR107 (GPR107) 20.8
 LDTP18448  Transmembrane 9 superfamily member 3 (TM9SF3) 20.8
 LDTP03810  ATP synthase subunit gamma, mitochondrial (ATP5F1C) 20.1
 LDTP11927  Essential MCU regulator, mitochondrial (SMDT1) 20.0
 LDTP00970  Gasdermin-E (GSDME) 19.7
 LDTP09547  Scavenger receptor class B member 1 (SCARB1) 18.9
 LDTP15648  BRI3-binding protein (BRI3BP) 18.6
 LDTP05511  Cytoskeleton-associated protein 4 (CKAP4) 18.6
 LDTP06308  Mitochondrial inner membrane protein OXA1L (OXA1L) 18.6
 LDTP00958  TBC1 domain family member 4 (TBC1D4) 18.5
 LDTP09968  V-type proton ATPase 116 kDa subunit a 1 (ATP6V0A1) 17.9
 LDTP15967  Tetraspanin-10 (TSPAN10) 17.8
 LDTP05900  Metaxin-1 (MTX1) 17.6
 LDTP01745  Putative lipid scramblase CLPTM1 (CLPTM1) 17.6
 LDTP06426  Translocating chain-associated membrane protein 1 (TRAM1) 17.5
 LDTP00857  Syntaxin-6 (STX6) 17.3
 LDTP11844  Protein spinster homolog 1 (SPNS1) 16.9
 LDTP00592  Surfeit locus protein 4 (SURF4) 16.8
 LDTP06034  Lysosome membrane protein 2 (SCARB2) 16.7
 LDTP00477  Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23) 16.1
 LDTP12331  Endoplasmic reticulum membrane protein complex subunit 7 (EMC7) 16.0
 LDTP11095  Mitochondrial 2-oxodicarboxylate carrier (SLC25A21) 16.0
 LDTP01601  Activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1) 15.3
 LDTP11615  Protein tweety homolog 3 (TTYH3) 15.3
 LDTP10059  PAT complex subunit CCDC47 (CCDC47) 15.0
 LDTP08981  Cytochrome c oxidase assembly protein COX18, mitochondrial (COX18) 14.8
 LDTP00821  Mitochondrial import inner membrane translocase subunit TIM44 (TIMM44) 14.3
 LDTP14174  Sorting nexin-5 (SNX5) 14.1
 LDTP13824  Voltage-dependent anion-selective channel protein 3 (VDAC3) 14.0
 LDTP11213  Nucleoporin NDC1 (NDC1) 13.9
 LDTP16190  Protein FAM8A1 (FAM8A1) 13.8
 LDTP09788  Sorting nexin-19 (SNX19) 13.6
 LDTP12909  Mitochondrial carrier homolog 1 (MTCH1) 13.5
 LDTP03546  Translocator protein (TSPO) 13.4
 LDTP03284  ATP synthase F(0) complex subunit B1, mitochondrial (ATP5PB) 13.3
 LDTP11245  Derlin-1 (DERL1) 13.1
 LDTP10669  Melanoma inhibitory activity protein 2 (MIA2) 12.9
 LDTP01969  Transferrin receptor protein 1 (TFRC) 12.9
 LDTP09261  Major facilitator superfamily domain-containing protein 8 (MFSD8) 12.8
Other
Click to Show/Hide to Show 65 Protein Related to This Bioclass
Target ID Target Name Binding Ratio
 LDTP12565  Mitochondrial import receptor subunit TOM22 homolog (TOMM22) 99.7
 LDTP11076  Apolipoprotein L2 (APOL2) 79.3
 LDTP13828  Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) 47.8
 LDTP05160  Nucleobindin-2 (NUCB2) 43.7
 LDTP00850  ER lumen protein-retaining receptor 3 (KDELR3) 37.8
 LDTP14939  Membralin (TMEM259) 36.0
 LDTP13311  Poly(U)-binding-splicing factor PUF60 (PUF60) 35.8
 LDTP15067  Ubiquinol-cytochrome c reductase complex assembly factor 6 (UQCC6) 30.3
 LDTP16192  Mitochondrial fission process protein 1 (MTFP1) 29.4
 LDTP13902  Cytochrome c oxidase assembly factor 3 homolog, mitochondrial (COA3) 28.6
 LDTP01522  Reticulon-3 (RTN3) 28.4
 LDTP08000  Dymeclin (DYM) 27.9
 LDTP03520  Phosphatidylethanolamine-binding protein 1 (PEBP1) 27.5
 LDTP15420  Mitochondrial import receptor subunit TOM5 homolog (TOMM5) 26.5
 LDTP10056  Protein FAM162A (FAM162A) 26.4
 LDTP19780  Keratinocyte-associated transmembrane protein 2 (KCT2) 25.8
 LDTP18373  Thioredoxin-related transmembrane protein 4 (TMX4) 25.1
 LDTP14486  Clustered mitochondria protein homolog (CLUH) 24.6
 LDTP00281  Golgi integral membrane protein 4 (GOLIM4) 22.0
 LDTP04652  Eukaryotic translation initiation factor 5 (EIF5) 21.9
 LDTP13797  HIG1 domain family member 1A, mitochondrial (HIGD1A) 20.7
 LDTP05375  Nucleobindin-1 (NUCB1) 20.5
 LDTP19963  TraB domain-containing protein (TRABD) 20.5
 LDTP08224  Protein LYRIC (MTDH) 19.2
 LDTP15269  Large ribosomal subunit protein mL55 (MRPL55) 18.1
 LDTP16006  Optic atrophy 3 protein (OPA3) 18.0
 LDTP08874  Mitochondrial intermembrane space import and assembly protein 40 (CHCHD4) 17.9
 LDTP08606  Nurim (NRM) 17.9
 LDTP16080  Protein FAM114A2 (FAM114A2) 17.9
 LDTP14458  Tetraspanin-6 (TSPAN6) 17.8
 LDTP13086  Syntaxin-18 (STX18) 17.1
 LDTP07368  Tetratricopeptide repeat protein 19, mitochondrial (TTC19) 17.1
 LDTP19859  Small integral membrane protein 12 (SMIM12) 16.9
 LDTP14041  TRPM8 channel-associated factor 1 (TCAF1) 16.9
 LDTP11249  Telomerase Cajal body protein 1 (WRAP53) 16.1
 LDTP12412  Reticulon-4 (RTN4) 16.0
 LDTP09069  Kinetochore protein Spc24 (SPC24) 15.9
 LDTP16323  Signal recognition particle receptor subunit beta (SRPRB) 15.9
 LDTP09297  Large ribosomal subunit protein mL64 (GADD45GIP1) 15.7
 LDTP10965  Nucleosome assembly protein 1-like 4 (NAP1L4) 15.7
 LDTP06097  Reticulocalbin-2 (RCN2) 15.3
 LDTP01466  HAUS augmin-like complex subunit 5 (HAUS5) 15.0
 LDTP06068  MAGUK p55 subfamily member 2 (MPP2) 15.0
 LDTP09773  Protein FAM3C (FAM3C) 15.0
 LDTP03775  RNA-binding protein FUS (FUS) 15.0
 LDTP00887  Calumenin (CALU) 14.5
 LDTP04684  Nucleosome assembly protein 1-like 1 (NAP1L1) 14.3
 LDTP02927  Ganglioside GM2 activator (GM2A) 14.2
 LDTP09410  Protein bicaudal D homolog 2 (BICD2) 14.1
 LDTP02180  Neurofilament light polypeptide (NEFL) 14.0
 LDTP08364  Ankyrin repeat and LEM domain-containing protein 2 (ANKLE2) 13.9
 LDTP12468  Diablo IAP-binding mitochondrial protein (DIABLO) 13.7
 LDTP03352  Splicing factor U2AF 65 kDa subunit (U2AF2) 13.6
 LDTP05641  WASH complex subunit 5 (WASHC5) 13.5
 LDTP11911  Golgi phosphoprotein 3 (GOLPH3) 13.5
 LDTP01358  Pre-mRNA-splicing factor SPF27 (BCAS2) 13.4
 LDTP01075  Protein CutA (CUTA) 13.4
 LDTP06853  TBC1 domain family member 10B (TBC1D10B) 13.1
 LDTP02887  Transmembrane protein 11, mitochondrial (TMEM11) 13.1
 LDTP11134  DNA replication complex GINS protein SLD5 (GINS4) 13.0
 LDTP00500  Heterogeneous nuclear ribonucleoprotein D-like (HNRNPDL) 13.0
 LDTP11695  Kinesin light chain 2 (KLC2) 12.9
 LDTP12755  OCIA domain-containing protein 1 (OCIAD1) 12.9
 LDTP09881  TATA-binding protein-associated factor 2N (TAF15) 12.9
 LDTP13273  Ubiquilin-2 (UBQLN2) 12.9

References

1 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587